LOCUS       NZ_LR135450             6355 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Enterococcus faecium isolate E7114 plasmid 8.
ACCESSION   NZ_LR135450
VERSION     NZ_LR135450.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884989
            Assembly: GCF_900639665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900639665.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,307
            CDSs (total)                      :: 3,214
            Genes (coding)                    :: 3,062
            CDSs (with protein)               :: 3,062
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 61 of 152
            Pseudo Genes (incomplete)         :: 92 of 152
            Pseudo Genes (internal stop)      :: 32 of 152
            Pseudo Genes (multiple problems)  :: 27 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135450.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7114"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(<1..158)
                     /locus_tag="FSA55_RS16335"
     CDS             complement(<1..158)
                     /locus_tag="FSA55_RS16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_179293132.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLV"
                     /name="transposase"
     gene            889..1812
                     /locus_tag="FSA55_RS16340"
     CDS             889..1812
                     /locus_tag="FSA55_RS16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            1828..2367
                     /locus_tag="FSA55_RS16345"
     CDS             1828..2367
                     /locus_tag="FSA55_RS16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            3052..3225
                     /locus_tag="FSA55_RS16350"
     CDS             3052..3225
                     /locus_tag="FSA55_RS16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            3629..4063
                     /gene="bacA"
                     /locus_tag="FSA55_RS16355"
     CDS             3629..4063
                     /gene="bacA"
                     /locus_tag="FSA55_RS16355"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(4944..6092)
                     /gene="tnpB"
                     /locus_tag="FSA55_RS16360"
     CDS             complement(4944..6092)
                     /gene="tnpB"
                     /locus_tag="FSA55_RS16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(6109..>6355)
                     /gene="tnpA"
                     /locus_tag="FSA55_RS16365"
                     /pseudo=""
     CDS             complement(6109..>6355)
                     /gene="tnpA"
                     /locus_tag="FSA55_RS16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /name="tnpA"
     Typing          890..1812
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccactaaag taatgtgata cgattctgaa attctttcaa aagtagtttt ggcataatca
       61 gatatttcat catcaatcac ttgtctacga tatttcgtta ctaaaacaag gtggtaataa
      121 agaagaaata ctgaatgatt atttgtatct aattccatgt tatatcaact catttcttgt
      181 atagactgat tgtaacatgg aacaaaataa aaaggcaata cgccttagtt gtcggtcttc
      241 gagttaccaa cggtaaccct cataccgaac ggcattcatc acccacctat agaggatggg
      301 cgacttctgc ctaattctgt taaatcgaat tttagagggc atttagacaa ttttagggtt
      361 gttagggctt gccctgaccg cctgtaaggc gttttaagaa caagataagg gatttatact
      421 aggactgtct taaaatgtct taaacgaacg ctcagagctt ctcaaagggt tccttatgaa
      481 cggcttttag caaaggagcc ggaggcgact gcgcagtata atgtgagagc agagcgatca
      541 caccttttta gcgaaccgta gggagcaagg tttggaattg gcagttttga taaattcgcg
      601 aacctgttta gagaaaaaag gagtgatttt ttaggcggga aaattagcct cccctctttt
      661 tatatctctt tttatatctc tttttatatc tcttttatgt cggctgtgtc cttactctcc
      721 caagcaattc agagtttttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc
      781 ttttcaagta ggttttctac gcttttcaag taggttttcc gacattattg agtattttta
      841 taaagtatgt tacttttatt tcatacttta taaaagtagg agaagcttat ggcaaatgaa
      901 attgtaaaat atgaaaatag attaaatcat attcctctga gaaaatttaa tagtcgtgaa
      961 atgaacttat ttttttcaat tgcttcacgt gtacgtgaaa aaggtgtaga tgagattatt
     1021 ttttcatttc acgatttgaa aaaactaagc ggttatacag aacgtgggga acaatttata
     1081 aaagatctta gtagcacgta tgacaaatta ttaagtatta atgcttggaa tgatgatgga
     1141 aatactttaa ctaaatttgt tgctttcaca aaatatagca ttatacgaga taaacaactt
     1201 gttataattt cagttaatcc agaatttaaa ggacttttta atcaattaaa gaactggaca
     1261 agatttaatc tagagcaatt tactaatttg gatagcacat attcaaaaac tatgtttcga
     1321 ttattaaaac aatatcgaac tcaaggctgg actgaatttt ccaaagaaaa attttttgat
     1381 ttactagata ttcctaaaag ttattggaat agtccgtcaa atattgacaa atttatctta
     1441 aggccaatta aagaagaact cactccctta tttaaaggat taactattcg taaaaaatat
     1501 ggtaaaggtc gaggtaaacc tgttatcggt tatcgtttta cttggaaagc tgaaattaac
     1561 catgctgacg atttttccaa agggaaacaa gaagatttac gtataaaatt atttaatatt
     1621 gatcacaatg gagaattaac acgagaagaa aaatggcgcg ctaaagatcg aatactaaat
     1681 cttcccttag gcactcatga agctgatttt aaacaacaac aacaaactga agaaaaacag
     1741 gcagaaaaac gagaactttc aaacaaactc aaacaagatt tactaggaaa tttacaaaac
     1801 ttattcgatt aagcttaagg agtatttatg gcagataaaa caatcaaaga acttgcggaa
     1861 gaattaggag ttagtaaaac agcaattaac aaaaaagttt ctgacaaaaa tcgaaaactt
     1921 tggtttgcaa aaaacggaaa caagtttgtg attaacgaaa ccggtcaaaa agctataaaa
     1981 tcaatgtttt tagtcgataa cgcaaactta aatcgaaaac tagttggcga aaaatcaaaa
     2041 accaaaaacc ataaaaacac caaccaagtt tccgaattaa aactcctttc tttcctagaa
     2101 aaacagttaa atcgaaaaga tatacagcta caagaaaaag ataatcaaat aaaacaaatg
     2161 caaaaattac tcgaccagca acaacaactc actctacaag cgaaccaaca aattgagaac
     2221 ctacaagaac agctccattt aacttataca gaagaaactc caacaaccga atcaactgcc
     2281 ttttctgaaa aaaaagcaga caatacacaa ccacttattg aaaaaaagtg gtggcaaatt
     2341 tggaaatcta atagaaccaa tctctaaaat tttctagaag ttagttcgga tagtagagga
     2401 aaaagttgta ctacctgatg gggagatcac tcaaattttt atgagaaaga aaaaatagtt
     2461 tacaactgta aacaatcaat gtttatagtt gtaaactatc aaactatatg gtgacttatt
     2521 gcttaaaata ggttgaaatc tcactcaatt tttggtggaa aaacaaaata aattctaatc
     2581 gattttgttg aaaaagtcat aaaaaaacaa atgtgatttt aacaaaattt caaaaaaatc
     2641 gtttgaaatt tcgtttattt tttttgagaa ttttgatcat cgtagggtca ttgtagaatc
     2701 attatgagat catcgtggga tcatcgaata aagtatggga aaggcttgcg gtgcttggta
     2761 ttaagggcat tctccgcctc ccaggttctt gaccattttc tctcaacgtg gtaaaaatcc
     2821 cacttataag agaaaatggt caagaaccta aacggtcggc gaagaaaaca taccaagcac
     2881 cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa attttttaga acaacttttg
     2941 aaaatttaat tttgatttat atccgttcaa tttaaacatt tgataaactg aaggctagta
     3001 tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg aggtataacc tatgttaaaa
     3061 aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag ccccatgcta
     3121 ttatccgtaa ttggaaaaaa caattccaac ggagaactta aattaaattc aaattattac
     3181 aacagtccta acgaagaatt gtttgtaaaa gagacggata aataataaaa aagactgctc
     3241 tttcgagcag ccaataaaaa ataatgagcg tttattcttg aaaaagcagc gcgcttatga
     3301 agcgctgcca aaaatttacc atattttatg aaaaataaca agtttctaaa aaggaatcgg
     3361 ttaagattca caaagaatcg taaacagaca atagcggaaa atcaagcttt tgtacgtgtg
     3421 ttttaactag accaatgaga aatcacaaag tccgtccctg cacccgttgg tagaggggca
     3481 acttgggcta gtacacatta tgtctactaa cctttaaaat aacacgtttt ataaaataaa
     3541 ccctcttgac aatatttaat tttctgatta ttctaaatat atacagaaag cgctttccta
     3601 ggattttata tctaaggagg aagtaacaat gaaaaataaa attattaaaa ttattacttg
     3661 tctatttttg ttcggtatga ttgttggagc aagtactccg ttaaatctgt ttccttttag
     3721 tgctagaggt ataaaagtgg aagctgcaag ttcacgatat aatcataatc atcgtggatt
     3781 tacttgctat aaaatgaact attatgttac taataaaatg tgtagagatt tgaaaaaaaa
     3841 ttataataag ctaaagactc ctgctcaaat tgcatctttt attcctattg gtggagttgg
     3901 aacatgggtt attacaaatg cttttagtag tgctgttgat gcaatgaatg tctttgtaag
     3961 agctgcaaat caaggaaaag gtgtacaatt aacatataat gctcacttta gcaatacaac
     4021 atcatatcaa tacaatgatt acgctcgtta tgtaataaaa taatcagaaa gtgaaactat
     4081 ctcatgaata aaaagatttt agctgtaatt atttcaataa tagttttaat cactatgttc
     4141 ttcatattta ggctaatttt caatataagc ttacaaaaaa gtattcttta tctaattcct
     4201 atagcattta tcttagcaat ttttagaagt atttacggaa ataaaaactc taaatagcta
     4261 aaaataaatc atactaaatt ttacaaaaag tggctagcat tttctagcca ctttttgtat
     4321 tgtggagcgt gggttttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt
     4381 tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca aaccctttta
     4441 gtgagttttg tagaaagtac ctttaaaggt actttcacat tacgaacgat acgtccgtag
     4501 gacgtactga aagggtggga cttatgagca aattaatgca aaatttggct agcaaaaaag
     4561 ctaaaaaata tatgctaata atcgagcgaa ataaacactt tttcaagtga gcgaatccgc
     4621 catgacaaag ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccga cttttttaaa
     4681 agtctaggca cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga
     4741 aacgcctaag tatataaata tataagaata ggtttaagag tgcgtgaact actcaccact
     4801 tagctaaacc tgacggttct taacgcttga agtgggagct tcttgggaat agagcatact
     4861 tgatagcgta tttctttact aacagcggtg tctcttattt accaagctat ccccgtagtt
     4921 cctacggttc ttgatgattg gatctaagcc aatcccatag aatgtagtct tagaccttcg
     4981 gtcagaatat tgatactagc attgatatca cgatcatgat gagtatgaca aacaggacaa
     5041 gtccattctc gaatttcaag agatttcttg ccatctttat gtccgcactc tgaacaaatc
     5101 tgactagacg gaaaccattt atctactttg atgatttttc gtccatacca gtcagccttg
     5161 tattgtaatt tagtcacaaa ctttgaccaa gaaacatcag aaatactttt agctaattta
     5221 tgatttcgca acataccttt tgtgtttaag tcttcaatac agataatatc gtgatttttg
     5281 atcatttctg tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc
     5341 aatctagcta ctttgcgttt ttgtttttga taatttttag cttcaaatag attggtacct
     5401 ttatttttag ccaataaagc acgcctagac aatttgcgtt gttcacgctt tagtttcttt
     5461 tccattttgg acgtgaattt attattatca attttttgtc catcagaaag aatcgcaaag
     5521 tccataatac ctaagtcaat accaattgtg gaattagttt taggtaattc gataatttct
     5581 tctttacaca acaaagaaat atagtattta ccgctagaat gacgtgatat tgtagcagat
     5641 ttgataatcc cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga
     5701 actttgatga atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa
     5761 ctctgaacag gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa
     5821 aaattcttgt atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt
     5881 tctttcaaaa acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta
     5941 gaagaatcat ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag
     6001 accttacgga cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag
     6061 attctgaatt tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt
     6121 gtttccttga ttttggatat acttcttaat cacatcaatt ggtgcgccac cagttgtcag
     6181 aagacaaaaa cttttagacc agaacatttc tttccaaaga aattgtttga ctcttggaaa
     6241 gtcacgtttt atcaatcttg agctggcact tttataggca ttgatgaatt ttgtcaattc
     6301 tgttttaggt tgagctttga acataatatg aacgtggtct ttatcatgat tccac
//
