LOCUS       NZ_LR135442             2056 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8691 plasmid 8.
ACCESSION   NZ_LR135442
VERSION     NZ_LR135442.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885878
            Assembly: GCF_900639655.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:12:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,795
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,634
            CDSs (with protein)               :: 2,634
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 43 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135442.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8691"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(<1..210)
                     /locus_tag="EQB50_RS14595"
     CDS             complement(<1..210)
                     /locus_tag="EQB50_RS14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127824029.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTK"
                     /name="hypothetical protein"
     gene            827..982
                     /locus_tag="EQB50_RS14600"
     CDS             827..982
                     /locus_tag="EQB50_RS14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1049..1651
                     /locus_tag="EQB50_RS14605"
     CDS             1049..1651
                     /locus_tag="EQB50_RS14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1787..2044)
                     /locus_tag="EQB50_RS14610"
     CDS             complement(1787..2044)
                     /locus_tag="EQB50_RS14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     Typing          complement(1710..1885)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          1050..1579
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tttggtggtt atgacttttt ctagtttttt aagggcttct gggctgaaaa gatagtttct
       61 tctaccggca gagcgatatt cttttccttc agtcagatgt tctttcgcta agcgcaaaac
      121 gtaagcttgg ttgtaatcaa gttcttgtgc aagttcgctg gtcgtcataa tttcggccaa
      181 agtgaatttt tctgatttta tcactctcat ttatgctcct tccatttaac gtccgtctat
      241 attacgtgag tacgtcggac ttacgacata atcttataat ataagttttc tgtgttgtgg
      301 tcaattttat attatcgaaa tgactagtag aaaaaatcaa tgctctaaaa agggtctatt
      361 ttgcgtttta agcgatttag cgtctatttc gtttagttat cggcgcaatc gttaaaacac
      421 gtaagagaag cgagaatgga gcctgtaagg cgactggtag cgaaacggtc gtgttagatt
      481 gcaaaagccg atagctgaac gaaatagtaa gcgtggcgcc tgcatatatt tcggtagaag
      541 gaggtcgggg ttcggggcgg agccctgagg tttgtttttt ggttttggtg tttatgaatt
      601 gggcggagga gggggggact acgacacccc ccttattggt cattggtcat tggtcgatag
      661 ctgtttagcc ttactctccc aagggtttca gcgttttttt ttgacaaaat cagtaatgcc
      721 aagggtttaa ggcctattat tcgctaatta gtatctaaac tgttgcacct tagttacttt
      781 tttgttacta tggtttttaa gaaaaagtcg ttggaggata cgaattatga tcagtaatga
      841 aaaaaagaga gtcgttgtaa cgcttactaa ggaagtggca gataatcttg ataaaattgc
      901 gaaacaaatg ggtcttagta agtctggtct tattacaatt tgggttaatg ctaacggaaa
      961 ggaaaatgag caaaaaaaat agcaaaagca ttagtgttgg cgcactgctt ttgctgttag
     1021 actgttgttc aattaaggta attatagcat gacgagtaga aaaactaaag atgaacgcac
     1081 aaggaattgg acgtttgtag tctatcctga atcggctcct gaaaattgga gggagttttt
     1141 agatgagtta catgttcctt gggttgagag tccacttcat gataaagatg tgaatcctga
     1201 cggtgaattg aaaaagcctc attggcatgt tttgataatg tttagcggta aaaagacgta
     1261 tgatcaaata cgtgagataa ctcaaaagct tcggtctcca aacccgcaaa aatgtgctaa
     1321 tgcaaaaggt atggtgcgat attttgctca tatggataat ccagaaaaat ttcaatatgc
     1381 taaaagtgat attatcgctc atggtggtgc agaaattgct agttatttgt cagtaacgag
     1441 tgcagaacga tatgagttga ttagagagat gatgtcgttt gttgattcta agaatatcac
     1501 tgaaatcaaa gatttaattg actatgcaat gtcagaaaga tttgatgatt ggtttccgct
     1561 tctttgtgat aattcagctt atattatcgg gcaatatata aaatcgaatc gtcatggtgg
     1621 aagtgtaaat tcaaaaataa ataaaggata agagctgtta atttcagctc tttttttgtg
     1681 cgtgaaagtc ttagtcacta tgaccaaaac tacggacgaa actatgacca aaagtaggta
     1741 ctatattatg accgaaaata ggtccaaaat ggattttttt gtgatatcaa aaaatataag
     1801 gttgttttgt aagaaaacca gttggcacag ccgacttagc caccttcctg taggaaggtg
     1861 ctatcagttt tcccttatgc tctttttgtt cttcagagtt taattccaga atatttgtga
     1921 gaagtaggtc aaagaggtcg gtggtttctt cgacttttcc gccggtcaat tcgataaaat
     1981 cattggaata tgattctagc aatttttcta gttgttcaac gttgatagtt gtttctttag
     2041 gcatttactc accttc
//
