LOCUS       NZ_LR135441             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8691 plasmid 7.
ACCESSION   NZ_LR135441
VERSION     NZ_LR135441.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885878
            Assembly: GCF_900639655.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:12:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,795
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,634
            CDSs (with protein)               :: 2,634
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 43 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135441.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8691"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            <1..249
                     /locus_tag="EQB50_RS14570"
     CDS             <1..249
                     /locus_tag="EQB50_RS14570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226817365.1"
                     /translation="IVRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1321..2040
                     /locus_tag="EQB50_RS14580"
     CDS             1321..2040
                     /locus_tag="EQB50_RS14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(2177..2593)
                     /locus_tag="EQB50_RS14585"
     CDS             complement(2177..2593)
                     /locus_tag="EQB50_RS14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2596..2880)
                     /locus_tag="EQB50_RS14590"
     CDS             complement(2596..2880)
                     /locus_tag="EQB50_RS14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     Typing          1322..2040
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attgtgagac tggagaaaga aaaaatggaa aaatttcctt acgaatattt tcaaaactta
       61 gaaaaattag agcaagcaat tcaagcaatc aagttcgatt taacagacga aaaattgttg
      121 gaatataaaa atatgacacc cgaaagaaaa cgatcgttgg aaaaattaat gcaactagtg
      181 aacgaagacg gcggaatttc agacgcagct tttgacgcat ggagcgaatt tcatggagaa
      241 tataattaag tgggtttaaa tggctctaaa atcgcttcta agagtttttt agcttggacc
      301 aatgaaatac accaaaaact aaataaaacg tcttaaatcg aaaaatacga gctctcagag
      361 cgactttatt tgtaaatgtt gcatataaga aataattacg gattttagac ttttgtagga
      421 tatttttgaa ataaatattt atttctgtta taatttgatt gcaaataagt gaacacgaaa
      481 cacacgaaag ccccagagag gtcggattct ctggggcttt ttttgtgggc tgagtcgcct
      541 taattaagtt ttacttcttg tttcggttat tcaaccagta actgaacaaa gcgcttaaaa
      601 gcccaacaag aagggagcaa ataaagttag tgaacacgaa acacacctcc ttccagtcgc
      661 aagactgtcg gataggcgac aatcaattat acaataaaaa agcaaaaata agcctaagtt
      721 ttttattgcc ttggggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttagaacga
      781 aggacatagt ttttatcgaa tcttgtctta gaacggctta gacgagctct caggacgcca
      841 caaaggagtc cctaatgaat tatttttggc gaaggagcct aaaggcgact gctgcaagta
      901 aaatgtgaga gcgtagcgat cactccttct tacagcgacc atagggagct agattggcag
      961 tttaatatca tgcgcgaacc tgtttatttt aaaaagtggg tgattttttg gcgggaaatt
     1021 ttgctcgcgc gcgcgcgttt attttattta ttttttaaag attttttaga acccttttta
     1081 gggggcagtt gagccttact ctcccaacgg atacagaagg gtactaacaa ctttttatct
     1141 gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac taacaacttt
     1201 ttatctgagt actaacaact ttttatctga gtactaatat ccttttgtat aataacaacc
     1261 aacctattta cgagttggtt gttaaaggtt attatttatt tgtaaggggg gaagaaagga
     1321 atggatgaaa aaacggaatt aacagtcaaa tatcaaaatg aattgaattt agtaccgctg
     1381 aaaaatttca atgctaagca gatggattta ttttttgcat tatgcgctcg tatgaaagat
     1441 cgtggtttga aaaaagttag attttctttc gaggaattaa aagagttaag tgattataag
     1501 atgactgcta caaaagcctt tgtcggtgat ttagaaaagc tctataaaga tatgcttaat
     1561 cttacttacc gtactgaaga tgaggattct attcgctact ttgttttgtt tactggtttt
     1621 gaaatcaata aaacaaaaaa gtttgttgaa gtcagtgtta atccggacct agaatatatt
     1681 attaatggtt taacagctga atttagtaga tttgaattat ctgctttcac gtcgttgcgt
     1741 tctacgtatg caaaaactct attccgactg cttatgcagt accgttctac tggttattat
     1801 gtagttcgta ttgaagagtt taggagctta atggatatcc cagaatacta tcaaatgggt
     1861 aacattgatc aaaaagtatt aaatcctgca ttacgagaac taaaaaaata cttcaatgat
     1921 cttaaagtca caaaaataaa agcaaaaaag ggcaataaaa tcgccaaatt agaatttact
     1981 ttttcaagcc taacgtcaga tttgccaaag atttctctgc gtaactggtt agaagaataa
     2041 ataaaaaaga actcgccccc cttacattaa gcaagtagac aagtccctta caaccgacta
     2101 agattgctag tacattctag catattgtat ggggcttcta aataaaaaac agataggaat
     2161 aactcctatc tgttttttat cgttttagaa aatttgttgg gtcgaaattt ctagaagtga
     2221 ctgtttgact ggtttctttt tgatgattct ttggtcgatt tcgatttgat ggatttagtt
     2281 tagcttcgat tttttctttc acttcttgat atactttaga atttgtgcca ataagaacgc
     2341 ctgtatcttt tagatataaa tcagcaaatt ctgcgatgat ttcagtcatg atggaaattt
     2401 cttttcccat agcatttagt tttgcctgat tgctggtagt ggtcgtgttg gtatcgggtt
     2461 cataactcat acataaaaag cgaatggctt ccgagtagtt attgatatct ggattttttt
     2521 cgatgatgga atctaaaacg tcaacatgac tagggtacaa agtaacatgt ttttggacag
     2581 aagtttttct catggttata tcattcctcc gataagcatt tcaatgattt gattatttgt
     2641 gtagacatac tcgcttaata tttgagtatt ttctgtttgg atagcaataa atttatcgag
     2701 caataaatcg aattggtctt ttttgtaagt cgtaagttga cggtcaaaat cactttcgat
     2761 taaaactttt agatatttat ttcgactcca tttttctcct gttttattcg tgagttcttg
     2821 gcaacgtttg tctattgttg caacaaactt tggatcaata ttccgaacta aaatgtccat
     2881 aaaaaatctc cttttgcaat tatgtataat ttatgtatac catacaaata ctatatttgt
     2941 atagctgttt tttgccaaaa tgctatacat aagctaccca cgatggcgaa aaagtgatat
     3001 caaatttttt tctgagaaac gtgataggca cagcctatct acccactttt tttagaaagt
     3061 gggtacacgt ttccttatgc tctttaaaat tcggggggga gtgctgcacg tgtaatcgat
     3121 aaccaaattt aaaaatgcac gatcaaaaat ttatttagtt tttcaaacga catttttttt
     3181 gagagtatag acggcaaaga agtgatttta tttctagaaa a
//
