LOCUS       NZ_LR135440             4065 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8691 plasmid 6.
ACCESSION   NZ_LR135440
VERSION     NZ_LR135440.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885878
            Assembly: GCF_900639655.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:12:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,795
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,634
            CDSs (with protein)               :: 2,634
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 43 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135440.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8691"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            <1..455
                     /locus_tag="EQB50_RS14540"
                     /pseudo=""
     CDS             <1..455
                     /locus_tag="EQB50_RS14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /name="recombinase"
     gene            904..1878
                     /locus_tag="EQB50_RS14545"
     CDS             904..1878
                     /locus_tag="EQB50_RS14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_010727957.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1875..2108
                     /locus_tag="EQB50_RS14550"
     CDS             1875..2108
                     /locus_tag="EQB50_RS14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127824028.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVADSSGEAND"
                     /name="hypothetical protein"
     gene            2179..2517
                     /locus_tag="EQB50_RS14555"
     CDS             2179..2517
                     /locus_tag="EQB50_RS14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176747811.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLKLPAEDNYSE
                     MLERMMDVLNSTINAGLAKSSVLTNEFVHRYLAQTTDEAEKEFWLKLMSHVMQLNQISV
                     INSKEKGM"
                     /name="hypothetical protein"
     gene            2521..2883
                     /locus_tag="EQB50_RS14560"
     CDS             2521..2883
                     /locus_tag="EQB50_RS14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323570.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNKSQ
                     MSESKFTSMFSDVDLR"
                     /name="hypothetical protein"
     gene            3105..>4065
                     /locus_tag="EQB50_RS14565"
     CDS             3105..>4065
                     /locus_tag="EQB50_RS14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_197724730.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYISKQREKNEREK
                     ALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLTSFE"
                     /name="plasmid recombination protein"
     Typing          983..1878
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..452
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.667"
                     /coverage="99.0"
                     /evalue="1.23e-87"
     Typing          3013..4064
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.145"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aattaccgga tggctcgtat cgcttatcta gtgctgattt tgtagatttg tatcaaaaag
       61 cgtctaatgc tacgaacagc aaaaaatatg ctgatatggc gaatgaatcg cttggaaata
      121 cattagataa aaatatcgct ttatttgata aaaacatggc gttagaacaa gaaaattctt
      181 ttttgaaaaa tcaacaaaag gttgatttgt cagcggttgt tgctgaaaac aaaaagctga
      241 aggaagaaaa tcgttctttg cgtcagcggt taactcaact gaaaaacgca tttcaaaaat
      301 caatcactcg tttatcaatt cgttttgggc ttgtagaaaa agatatggga atatctgaag
      361 agctaaaaat attagatcaa gagaaaacgt tggctagtca gcagaatgcc ccagaacgta
      421 atatagggcg ttctgagaat gagatggagt ggtagttata cggtgcagtc aacggtaccg
      481 gaggcggtct gtgccgagga taaggagttg acggctaaac cactaatccc tgaagctgac
      541 atttgctaaa aaatggcaag tccatttttt ggccaactta gcgttagcga agtttggggg
      601 cgcccttttc aaagggtgcg gttttatgtt tttggatatt tgcatagcta aagagaagtg
      661 ctattattga gttatcaaat aaataaactg aaaaaagagc aaaaaaataa agtcgagcaa
      721 cgctggacac gttctcgacc tttacggaaa ctaatcacac tagctccgta actcaaattc
      781 attatacgaa taatttcagg tttcttcaag actagagatt gctggttatg aattttttag
      841 agttacgtga actctggaaa attctttcca ccaatctctt ttttctttga ggaggaacta
      901 aatttgagta aaaaaggcac gatcataaat gagtgggaaa aattaacagc cccctctaac
      961 aggcggttaa gtgtcccttt tatggacacc cccctaaaaa ttcagtggtc aatcgaccga
     1021 attaccattg ttggaaatct taaagagaat atctattatc acactacaca tgatgtgttg
     1081 attttagatt ttgaacagct tatgcgccta aatgaaggta acggttattt aagatctgtt
     1141 agtaataatg gttggcagtt gttagaccaa catgaagaga atattgctta tattgaaatt
     1201 ttaaaatggc aagaggggaa agggcgtata gacttcaatc cgaataagat tagtcaattc
     1261 ttagctagtt ccatgaaaaa ctttattcat gatttgttta tcgaacctca cttttcacgt
     1321 gcagatattg cttgcgacat tatcgatgta cctgatgagt ttattacaca atatcgggta
     1381 gttgatccag tttcttttaa gcctatttat ggacggaatg gaaaattaga gacggcatat
     1441 tggggatctc gctcttctga acgtcaaatt cggatgtata ataaaaaact ggaacaagag
     1501 aagaaaagaa aaattgtgcc aaaagaaatc gtgagttggt ggcggttaga attacaatta
     1561 agacgaggga aagcgactga ttggcatgca atggttcaag aaagcttgga tagttttgct
     1621 agtcctcatt acttgcctgc tgatacaagt gtagctgaca aaatgatgat tactgcttta
     1681 acaacagaac atgattattg ggggcaaatt aatcgaaaga caaaatataa atatcgaaat
     1741 ttgctgaagc aagaaagtca aaatgatgaa ttgacgaatc atttacgaga aacctttgca
     1801 gaatcggcag acgatttaaa aaaagaatta gacacatggc ttctagggct agacgtaaca
     1861 gaggaggaag aaaaatgaca gaaaaacaaa agtattttgg taagaaaaat tggttggtaa
     1921 atttaacagg tgattacgtt gtggttgttt ttttggatag caaaaaaatg tccggtaagt
     1981 tacttgatgt aacgcaatat gaattgattt tgaaaaataa taaaattgac tctccgatta
     2041 ttatccctaa gcacgccgtt aaatatattt atgcggtagc tgacagtagt ggagaagcta
     2101 atgactgatt tgtcagaaaa gatgaccata aaaaatcttg ctgatgaatt aggtgtatcg
     2161 aaaattaaag aatcggaaat ggtggcagtt ttggaaatga ggtgtaacat ggataaaatt
     2221 gatttaagag aagagacagt tgatagaatt gaattgatgt atgagcggtt agctgattat
     2281 ttgaaattgc cggctgaaga taattacagt gaaatgcttg agcgaatgat ggatgtttta
     2341 aattccacaa tcaatgccgg tttagcaaaa tcttcagttt tgactaatga atttgttcat
     2401 aggtatttag cgcaaacaac tgatgaagct gaaaaagaat tttggttgaa attaatgagt
     2461 catgttatgc agttaaacca aatttccgtt attaattcca aagaaaaggg gatgtagttt
     2521 atggggatga ctttagcaga tattaaagca caagaagaaa aattgaaaaa agaacaggca
     2581 aaacttcgtc agcagaaaaa acgattgatt gagaaggaaa atgctgaaat gggtaaggca
     2641 cttcggaagc attttggatt tgaatctttt gatgattttg agcggtggtt atcgtcaatt
     2701 tctgacgatc agcaacacga aaaatcagtg gataatgagt ggcttgctga tgaaaaatca
     2761 gcgccaagcg aacgagatta tctaaacgaa tctgacggct tattcgaagt gataccgaat
     2821 aacaaaagtc agatgagtga gagtaaattt acgtcaatgt ttagtgacgt ggatttacga
     2881 taagcgatta ttttgcgtga agcaaaataa agcgtaaaag gagttgagag cgtgacacgc
     2941 tttcggctcc ttttttcgtt gtcacttgtg acagcgaata ccctcggaga gcatttggaa
     3001 ttgtgacagc catgtcacaa ttaggaaaat gttactcttt gacaaagagt aacagaaagg
     3061 tttacactct ttttattagc gacagcgcga aggagggttt taacttgggg tttagtattt
     3121 catttaaaaa aggaacgaag aatacaagta tttctcataa taatcgtgag ttaactgatc
     3181 gccagaaaac caatgattgg cataagcaca ttgacttcac gaaatcggat gaaaatattt
     3241 atttagagca gacgaatcta gcagataaat atgaggaatt gtttggcgaa gtggtagaaa
     3301 aatataatgc gaaacaaaaa agagccgata gaaaaatcga ggactattta gcgaaagtgc
     3361 gcaaggataa aaagttggaa cctcaacgag aatttattgt gcaaataggc attttagatg
     3421 atttccagac aaatcgagca gatggttctt ctactggcat atcgaaacaa caagctgaac
     3481 aaaatcgagt gattgccaat aaaattttgg ttcagtatta caaggagttt caagagagaa
     3541 accctaattt ggctgtttat aatgctgtta ttcataatga cgaaataagt ccgcacctgc
     3601 acttgaatat tgtgccggtt gctgaaggtt ataaacgggg tgttcaaaag cagccgagct
     3661 ttaataaagc actggttcaa caagggatac acgctgataa agataatggt cgagtgcttt
     3721 tcaataattt tagaaatcaa gaagttgact ctatagaacg attaatgaat gattacggtt
     3781 ggaaaaggga tattgtcggt accaacaaaa ttaaagatat tcacgaatac aaagaaatca
     3841 tgagtgaaat ttctgaattg cgccaaacgg ctgtaaaaga gcgtgagagc gtttctgcgg
     3901 aaatgaggta tatttctaag caaagagaaa aaaacgagcg tgagaaggct ctggtggctt
     3961 ctgagaaggc gaaaattgaa gaagagaaaa aatcgattat cgatttggaa aaagagctgg
     4021 ctgttgtttt tcaaaaagac cctgttcctt taacgagttt tgaaa
//
