LOCUS       NZ_LR135438             9323 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8691 plasmid 4.
ACCESSION   NZ_LR135438
VERSION     NZ_LR135438.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885878
            Assembly: GCF_900639655.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:12:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,795
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,634
            CDSs (with protein)               :: 2,634
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 28 of 72
            Pseudo Genes (incomplete)         :: 43 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135438.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8691"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            <1..563
                     /locus_tag="EQB50_RS14435"
     CDS             <1..563
                     /locus_tag="EQB50_RS14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_164851640.1"
                     /translation="ARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            591..1130
                     /locus_tag="EQB50_RS14440"
     CDS             591..1130
                     /locus_tag="EQB50_RS14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1146..1316
                     /locus_tag="EQB50_RS14745"
     CDS             1146..1316
                     /locus_tag="EQB50_RS14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1634..2095)
                     /locus_tag="EQB50_RS14445"
     CDS             complement(1634..2095)
                     /locus_tag="EQB50_RS14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2228..2602
                     /locus_tag="EQB50_RS14450"
     CDS             2228..2602
                     /locus_tag="EQB50_RS14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            2656..4782
                     /locus_tag="EQB50_RS14455"
     CDS             2656..4782
                     /locus_tag="EQB50_RS14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            4779..5399
                     /locus_tag="EQB50_RS14460"
     CDS             4779..5399
                     /locus_tag="EQB50_RS14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            5513..5650
                     /locus_tag="EQB50_RS14750"
     CDS             5513..5650
                     /locus_tag="EQB50_RS14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            6092..6472
                     /gene="mobC"
                     /locus_tag="EQB50_RS14465"
     CDS             6092..6472
                     /gene="mobC"
                     /locus_tag="EQB50_RS14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            6454..7368
                     /locus_tag="EQB50_RS14470"
     CDS             6454..7368
                     /locus_tag="EQB50_RS14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7328..7606
                     /locus_tag="EQB50_RS15155"
     CDS             7328..7606
                     /locus_tag="EQB50_RS15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            7608..8297
                     /locus_tag="EQB50_RS14480"
     CDS             7608..8297
                     /locus_tag="EQB50_RS14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(8418..8504)
                     /locus_tag="EQB50_RS14485"
     CDS             complement(8418..8504)
                     /locus_tag="EQB50_RS14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9149..>9323
                     /locus_tag="EQB50_RS14490"
     CDS             9149..>9323
                     /locus_tag="EQB50_RS14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_164851642.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNI"
                     /name="RepB family plasmid replication initiator protein"
     BGC             1..7602
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..563
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6455..7365
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcacgtgg cgaccgtttt gctaatgatt taataaaaat gtcaaaaaaa atcattactt
       61 tgtcttttat ttatgaagac gaaaaaacat atgaacaatt aaatctgttt caaaaattta
      121 aaatcgataa agtgaatgaa acattattaa taaaggtttc tgaagaattt gaatatatac
      181 ttaattcaat cggaactaat tttactcgtt tcgaattaga aaatatgaca aacttaaaga
      241 gtagctacgt taaagagtta tatcgccaac ttatggctca taaagacaga agatctaaaa
      301 aaggtcactg gtttgtaaaa atagaagatt ttagagctct attagcaata ccagaaagtt
      361 atcgcatgag agatattgat aaacaaattt ttgcccaagc gaaaaaagaa tttttaaacg
      421 ttaattcaac tcaccctgct atttttaaaa cttttgaaat caaaaaaatt aaagcctaca
      481 aaggaaataa gataggatct ctgcaaattt attttgaaga aaatatacct tcaattacct
      541 tagataattg gttagagaaa taatacctaa caatttagga ggcaattatc atgcaaccca
      601 aagaaacctt tacaataaag gaattatctg aattatttga aataagtagg caagcaatct
      661 ctaaacatgt tgataaatta gacccgtcct tattagctaa aaatgagcgt ggttataagg
      721 ttgtattacg ttctggagta ttgcaacttg ctagcaactt aggtaaccaa aggttactat
      781 ataggttgca tgagtatgaa caagaaaacg ataaaaacgt ttatatatca agcaaaaacg
      841 tgattgtaaa cgagttgcta gataagttgc gtgaagaaaa tgaattttta cgagaacaac
      901 tttccctcaa agagcaaaat aattctaata tgcaaaaatt actcgaccag caacaacagc
      961 tcaccctaca ggcaaatcaa cagatcgaaa aactgcaaga acagttacaa ttagcttata
     1021 cggaagaaac tccagaagat gaatcaacta ctattctttc tgaagaaaca gaatcgatag
     1081 aaaaacacac aaaaaagaag aagcaaaaat ggtggcaact ttggaaataa ctggagggaa
     1141 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggcttca
     1201 gtccgatgtt attgtcagtg atcaataaag gaaataaaca tggtgaattg aaattgaatc
     1261 caaattatta caacagtcct aacgaagagt tatttgtaaa agaaacggat aaataataaa
     1321 aaaagactgc tctaacgagc agcccaaaat gagattatcc tagtttagca acataaacca
     1381 tttattagat gaaaatgaga tacaaacaaa gcgtatcaaa tcctctcaaa aataacaagt
     1441 cttaaaaaaa tgacagataa aaatgcacaa agaattagat atagataacc gcgaaaaatc
     1501 aagctttaga aagttgcttt tagctagacc aattaaaaat cacaaagtga gcgtctctat
     1561 ccattggtag aggggctact tggcatagtg tacattatgt caactatgca attaaataac
     1621 taactacttc atattagctt aataaaataa tcaaaataac tagggggaaa aacattgtat
     1681 aaaaaaattt ttcatttttt aaataagaat agtaaatata aaaataattt aagatatcta
     1741 ttactattaa tccagataga gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat
     1801 tcatttgaat caattcaaat aaacctaata tagtaacaaa tatattaaaa ataatagctg
     1861 tcaataccag taaattattt tttttatttt ttgaattatc ttcatttaaa atagaattta
     1921 tgttagtact ataaaattca caaatttttt ttagtgtctc tatatctggt gttgttttgt
     1981 tattttccca ctttgaaata gtttgacgtg taacgttaaa ttttcttgcc acatattctt
     2041 gagtataccc ttttttcact cttaattctc ttagcttaga tcctaaatcc tccatttttt
     2101 ctcctatgta aacgattatt tacataaaat tttatcataa aaatactaac gcgccaaaaa
     2161 agttctttat tttataaaac acactaaaat aaaataattc tagtaaaaaa gataggagaa
     2221 ataagttatg aaaaaatttt tatgtctttc tgctttaatg ggtgtcttat taactagtgg
     2281 aggaatagct agtgccacag aagctttaaa tctagatgta cccgaagacc ataccgccat
     2341 ttacggagga ggaatggaag ctacagaaga aggattttct tctagtaaat tgagatatgc
     2401 tgctggcggc ggggatttca actgtggggt taatggattt aaagtttatg caaattacta
     2461 ccatgctaga gctaaacata gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc
     2521 agttcagaaa gctggagtaa gagcttatgc aacatgcaat gccacactta ctggaaatac
     2581 tggttggtgg aatgtttatt aaaaaagaga gtttaaactc tcttttttat ttacatttga
     2641 aaggaaattc aaaatatgtt aaaaaaaata aacgctgttt tatcactttt tgtctgcatt
     2701 acaatactat ttttttctac taatgttata aatggtataa agacttctga agcgctatat
     2761 aaaaataccg ctgaaattca tatgcaatat aaagatgata tggactttaa cagttatata
     2821 aacgctttgt atgaaatatc tacacaagaa aatattaata tttctcaata tagttttact
     2881 gctgttaacc aactaactat tcttgctaca aaccctaacg ctaacaaaca ctggaaattt
     2941 tctaaaaaaa atatattagc aagaaacgat tctaaagtcc attatactaa taacaaaaat
     3001 tctaagtacc aattaaaact tccaaataat tttttaaata taaatattta tcctttttct
     3061 catgtggaaa atattggatt aagtgaaata ttatatgtac aaggaaactc aaacaaatta
     3121 attcctgtac tgaaaaaata tggaacacct actatttcca atatcaatca atcagatact
     3181 tttcaaatta atactaatat tgttcttgtt atctgctatc ttagtatatt tattgtaatt
     3241 actacaatta tatttgcttt ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc
     3301 tataactctt ttgatactat aaaagattct attaaagagt taaacctctt attttgttct
     3361 ttgattataa ctttcatttt atctattttt ttgagcatat attacagtaa cgaaaatttt
     3421 ttcctatcat ttcttctatg tagtattttt cattttataa ttagtttttt atttatcata
     3481 gttactctaa gtatctatca tttatctagt ataaaaaatt atctaaaaaa ttcacgacca
     3541 cttaagttaa atctttgtat attaaatatc tgtctattct taagcatgat ccttctgtta
     3601 atagcttcta caaaagtaat aaatacccat aatgaagccg aaaataattc tcttaaatat
     3661 tgggaaagaa ctactaattt atacaaaaca aatattacta atcaattaaa tagaaacaac
     3721 accgtagaag aaaataacta cttgaaaaaa gctagtaaat ttgtttataa aatacagaag
     3781 aattataaaa cttttattat cgcgccatac aattacgcaa ctatccaaga gaataataaa
     3841 gaatttttta ttggacaaaa agtttatcca aattttgaag actatgttag tcaacctgct
     3901 ggtgcaggaa tatgtgttga tttaaactat ctaaaatatt acaatcctat ttcttttgaa
     3961 gaatctactg acttaaattt aataaactct gatccactaa ctacttacat tctagtacct
     4021 aaaaaatata aagaatatgc aaaaatgatt gaaaaaaatt atttagaaga catacagttt
     4081 agacttgagg actctcctcc tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa
     4141 ataaaactaa tatttgttaa taataaccaa aagtatttta gttttaatac cttatatgga
     4201 aaggcaaaag ataactatac aataactgat cccatagtta gagttattaa tccagaaata
     4261 acagaatcat tattttgggg aaatatattg actagtgatg gtggattatt ttttgatcag
     4321 aaacatactt caaaccaaaa cttttttaat caaattaatc cgtatataaa agaatttaac
     4381 ctagaaaata tcataaattt ttcttattct gtttttcaag aaactggtga attattatct
     4441 caaaaaaaag taaataaatt aaatgcatat ctacaatatc ttttaatatt aggattattt
     4501 ataataatta attttcaaat gatttttgtt agttttacta taaattcaaa aaaaatagct
     4561 ttaaaaaaaa tatttggata ttcaccaata agtcaagttt tagatttatg tttactccct
     4621 tttacaatag agttatttgc tactatgtgt tttcaattga tattttcaaa aaacacttac
     4681 ttgtatttta taatttttat gatcttttta ataaatctaa ttacttctat tattatttat
     4741 aaaaaaaata gttttaaata ttttaaggag aatattttat gatattatca gttgagaatt
     4801 tcaccctgtc aattaataaa aaaaagatca aacaaaattg gaacttttca atatcagaaa
     4861 aagattttat tatagtaaca ggaaaaagtg gtagtggaaa aactactctg ttaaataatt
     4921 taagcctaat agataaacaa tataccggta acttattcta ttttgaaaag aaagtttcta
     4981 aacatgatat acaacgtcta cgaagaaatc atattagcta tttatttcaa aactatgctc
     5041 ttcttgaaaa tcaaacagta caatacaatt tcgaattagc aaaaaagttt aacagtaata
     5101 tttccaatga tcatatttat aatttattaa cagatttttc tttgccaaaa gatatcttaa
     5161 aagaaaaaat ctttctctta agcggtggag aacaacaaag aatagcatta attagaaata
     5221 tgttaaaacc atttgatata ttatttgcag atgaacctac aggaaattta gataaagaaa
     5281 actcaacttt tataatgaac tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg
     5341 tactagtaac acatgactta aatgttctaa actatggaac taatattata gaattataat
     5401 ttttacccgt gatgctagct gatttaaaac tagcaccacg ggtatttatt ttatattcca
     5461 tctctcctat cccaaaattt aaataatcac ttaataaaaa ggaggcaccc aagtgatttt
     5521 attgaaaaaa gagtatgaaa aattttatga tagacttatt tattctttgc aattgccaaa
     5581 aaaattaaac cgtcaattac aaaagactta tgaaactaaa tcaactgaag aatgggagag
     5641 ttattgctag atttttggta gaagtaacaa aaagtggaca tattatcata atgactcgcc
     5701 gaaagtctga taaaaaattg gctaaaaata ttgctgaatg tttaataaaa ctatcagttt
     5761 tagctaaata gttagtagtt tacacaaaaa agcatttatg ttttttgttg caaagcaaca
     5821 atttcatctt cggtgaaata agcgaaactt tacaaaaagt ggctagcatt tgctagcttt
     5881 ttttgtattg tggagcgtgg cttttgaggg ggattcaagg gggagcttgc gcccctttga
     5941 caagcggtta tttgcactaa atacgtaggt atttggctag caaatataga gcttgccaaa
     6001 cccttttagt gagctttact cattgtacct ttttaggtac aatggtattg cgaacgatac
     6061 gtccgtagga cgtactgaaa gggtgggact tatgagcgaa caaaatcaaa atttggctag
     6121 caattcttct aaaaaatata cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag
     6181 cgaatccgaa tttgcaaagc tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc
     6241 tttcgtgaaa gccaaggcac aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat
     6301 tgcccaggca atcgcgcgcg atttagccaa agctggaggg aatatcaatc aaattgctaa
     6361 gtggtgcaat acacaccaac atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa
     6421 cctaaaaatc atgcaaaagg agctacaaaa gatatggcaa caattaaggt aagccgaacc
     6481 acttcttgta gtcgtgcaat taattacgca gaaccacgag caaccattaa aacaggaata
     6541 aactgtgacg tcgattatgc caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa
     6601 gacgaccgtg tacaggcaca cttattgatt caatcttttc gaccaggaga aattaccgct
     6661 gaaaaagcta atcaactagg ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata
     6721 gctatttaca cacatacaga caaagaccat attcataatc acattgtcat taactccgtt
     6781 aatctggaga ctggaaaaaa atttcaagct catggtcaag catttctaaa caaatgttat
     6841 gacctaaatg acgagatttg tcttgcacat aacttaccaa tcactgagcg aggaaagaaa
     6901 ccagaaaaac gaaccatgtc tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa
     6961 gacgaaattc ggattgcgat cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac
     7021 caattttgta attccctcaa aacattgggg attcattgct tcaatcgcgg gaaaaatttc
     7081 acttacgaat tgatccacaa aaagaaaaaa gtacgatcca acagactagg aaaggactac
     7141 gaaaaggaga cgattttaca tgagctggac agacgagaac gaacctataa ccaacagcga
     7201 accaacgagc tacgaagagc tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga
     7261 gaacaacgaa ctcttacgcc tgaacaaccg gaaattagac cggctcgaag aaaccatgaa
     7321 caacacgttg aaagaggaac agaccaccaa caagaattat cgctctaaaa cgttaaaaga
     7381 catagaaaac gaaataaaga aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat
     7441 acaaactacg attgatttac aagttaaaaa actccatcaa gagctattag accagcgaaa
     7501 taaggaacag aaaaaggtgc ttattcaacg atcattggat tttcttgccc ccttatgttg
     7561 cttagtcctg acactgttcg cattttggtt agtatgggga ggctaaaatg gcattttatt
     7621 taccctatct actcattttt gttagtattt caggaagtat ttggctcatc tataaaattt
     7681 ttcaaacaag atattcatta aaaggctcta aaattcgatt taagcgattt ttctttcttg
     7741 gctgtatatt ttccttgata atcgtttcaa gcggcttatt cggcgtttta gagggaaata
     7801 agcgcgtttc tagaagtatt ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg
     7861 ataaaaagaa aaaagagcaa gcattagcac aaaaaatgga agagtttacc acttgttatg
     7921 aggaaatgaa cgatattttt gtgaatcaag aaaaacgttt gacggataaa aacatggaaa
     7981 aactcactcg tctttatcaa aacttgccag aaaaacagca aaaagaggtt caagacaact
     8041 atgagcgaac caaaaaagac gtgcaatatg taaaagatac aaagatagaa gaagcctgta
     8101 gtgatctatt tggagacaca aacccttggt ttgccagtga agaagaaaaa aaagaaaaac
     8161 aacaatctgt cacatatgaa cgctatgaaa tcttacttca acaagcaact aacatgcaaa
     8221 atccgacaaa aaaagaaacc gccttaaact atctaaaaag tgtcaaggaa tggttagatc
     8281 agcaacaaca aaattgaatt tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa
     8341 acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt ttcaggttga
     8401 tgtcgccttt attgatttta cttcttgttt cggttattca accaataact gaataaagag
     8461 acacaaactc ctacaagaag ggggcaaatg aacagttcaa acattagata cacctccttc
     8521 cagtcgcaag actgtcggat aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt
     8581 ggtgttttta agtatcactt gttttagggt tgttagggct tgccctgacc gcctgtaagg
     8641 cgcttctcga ctagattaag ggaaattaat caagaattgt cttagaacgg cttagacaag
     8701 ctttcagagt ctcacacctc ctctttgaac aacggtttta gcaaaggagc cggaggcgac
     8761 tgcgcagtat aatatgagag catagcgatc acaccttttt agcgaccgta gggagcaagt
     8821 tttccagttt agaaaaattc gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg
     8881 ggaaattcaa tgtgctctta agatatactg ttagatatac tgttagatat actgttaata
     8941 ataactgtta gggattagca accttatggg agagtaagta ttgacatagg tagtggggga
     9001 taaattgtca cactatgggg gataaattgt cacactatgg gggataaatt gtcacactat
     9061 gggggataaa ttgtcacact atgggggata aattgtgttt tacatcctct tatgtctatg
     9121 ataacataat ttattgaggt gatttttatt gagtaacgat atagttcgat ataataatgg
     9181 attaaattct gtccctttaa gaaaatttac ccctatagat atggatttat tttggggaat
     9241 atgttctaaa atgaaaagaa aaggtacaaa agaagaaatt tttacattta aacaaataaa
     9301 agaattaatt gattacaata taa
//
