LOCUS       NZ_LR135433             5275 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8927 plasmid 6.
ACCESSION   NZ_LR135433
VERSION     NZ_LR135433.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885924
            Assembly: GCF_900639635.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:11:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,819
            CDSs (total)                      :: 2,728
            Genes (coding)                    :: 2,644
            CDSs (with protein)               :: 2,644
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 24 of 84
            Pseudo Genes (incomplete)         :: 55 of 84
            Pseudo Genes (internal stop)      :: 23 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135433.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8927"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            complement(<1..690)
                     /locus_tag="EQB53_RS14655"
     CDS             complement(<1..690)
                     /locus_tag="EQB53_RS14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_127823945.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLAQ
                     TQLEATRRIFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPNHE
                     IYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQPKE
                     KFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNKGKRI
                     GFKWVDDE"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(675..1031)
                     /locus_tag="EQB53_RS14660"
     CDS             complement(675..1031)
                     /locus_tag="EQB53_RS14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002323247.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLCK
                     SVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQQL
                     SQIEKGLIDLWEQL"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(1587..1952)
                     /locus_tag="EQB53_RS14665"
     CDS             complement(1587..1952)
                     /locus_tag="EQB53_RS14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322917.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFMR
                     NASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLDYK
                     TFYPELLEFFQKLNSLE"
                     /name="hypothetical protein"
     gene            complement(1961..2122)
                     /locus_tag="EQB53_RS14865"
     CDS             complement(1961..2122)
                     /locus_tag="EQB53_RS14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322916.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLTE
                     IIELDSKE"
                     /name="hypothetical protein"
     gene            complement(2112..2261)
                     /locus_tag="EQB53_RS14870"
     CDS             complement(2112..2261)
                     /locus_tag="EQB53_RS14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322915.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGEQ
                     KNDG"
                     /name="hypothetical protein"
     gene            complement(2275..3024)
                     /locus_tag="EQB53_RS14670"
     CDS             complement(2275..3024)
                     /locus_tag="EQB53_RS14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002322914.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVLD
                     YCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVERP
                     DGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGKYAL
                     IMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELSDKMS
                     VSFFVTTLKRGRKVVGYEITINDTSKE"
                     /name="replication initiation protein"
     gene            3739..3837
                     /locus_tag="EQB53_RS14675"
     CDS             3739..3837
                     /locus_tag="EQB53_RS14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110958386.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3991..4488)
                     /locus_tag="EQB53_RS14680"
     CDS             complement(3991..4488)
                     /locus_tag="EQB53_RS14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322921.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRVV
                     FALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQAL
                     KIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYFELN
                     SQ"
                     /name="hypothetical protein"
     gene            complement(4516..4971)
                     /locus_tag="EQB53_RS14685"
     CDS             complement(4516..4971)
                     /locus_tag="EQB53_RS14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322920.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGYI
                     KVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQVQ
                     KFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
                     /name="hypothetical protein"
     gene            complement(4979..>5275)
                     /locus_tag="EQB53_RS14690"
                     /pseudo=""
     CDS             complement(4979..>5275)
                     /locus_tag="EQB53_RS14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rlx"
                     /name="protein rlx"
ORIGIN
        1 ctcatcatcg acccatttga agccaatcct tttgccttta ttgtactcat aaacctcgac
       61 agcataggct tcttgaagat tttttttgaa agaatcaaaa tcggtactca tatcttgtgc
      121 gcccaggatc atatgcttca ttttttctcg attactcatt ttgccctcgg cgtgcatttc
      181 aaaatcaact tgtttgaatt tttctttcgg ctgttcaatg ccaaaactca ataaaacctt
      241 atcgtttagg tcttgtaggt ggtagccgac ctcggtatca gagtgaaatt ttttaccggt
      301 ttcagtgtcg atactggcga aaacaatgtg attatgaata tagccggatt ctgaatcggt
      361 atgagtgccc atgtagattt catgattggg atagcttttt gcgatttcat caacgagttt
      421 agcaccgatc tcatgcgctt tttgcacgtc cgccggattg tttttgtcaa acgaattatc
      481 aaacgattga acccaatgaa aggcttgaat accgcctgtt ttggcgaaaa tccgtctagt
      541 cgcttctagc tgagtttgag ccaatttatt ctcagctgac agtttgtgcg tataaatgcg
      601 gtcacgatca agctttccat gccgatttac atagccaatc agcgcagaag ccccacgttt
      661 accgcctttt aaagctaaag ttgctcccat aagtcaatca accccttttc gatttgcgaa
      721 agttgctggt tcatcaaggc taaatctgaa cttgaaggca tttgcccctt attcatgtta
      781 ataaagcgag ccatctgatt gagatttccg ccatatttgc gcaactcttt ggcaatttgt
      841 atcgccccct cccgatcaac gttcggcgct cgatacttca tgccaagtgc cacgctcttg
      901 cagagctgtg gcacactcat accagtttga tccgcaatgg cggtcaaata ctggtactcg
      961 tcttcgctca ctcgaaattt aatttgccga ctatcttttc gattttcggc attcaatttc
     1021 tgctcgctca tctcatcact cctcaaataa aaatgctccc tattcggtcg cttttttctt
     1081 ctcgtcattc ttcgtggtcg atattcaaag ctacaaaaac gctccctatt cagtcgcctt
     1141 ttttccgcaa tgaaatctcc ctcggttatg ccactacaaa atgaaagttc cctactcggt
     1201 cacttccctt tttccgctct ataaccgaat gatttattaa cctcatcata ctccctattc
     1261 ggtcgcataa caaggttaat aaatcatata atacgcaagc accgcatttg gtacccaaat
     1321 acatcgcttg tcgaggtggc gttgccccct acgaactccc ccaatcacgc aacaaaaagt
     1381 agcctgttcg gctattttta ttacgtgatt tcgcctcaga aaaagtagtc tgttcgacta
     1441 tttttctact ggctcatgcg gtcaaacttg cacgcaagtt gaccttattt ttttgtctgc
     1501 ccgctgttcg caggcaaaca aaaaagcaag ccaaaaatcg gcttgctcgt tcttcaaaaa
     1561 ttgatcgcat ggactagctc gttccgctat tccaagctgt tcaatttttg gaagaactct
     1621 agcaactcag gataaaatgt tttgtaatcc aacttgtttc cccagttgtc ttccatccat
     1681 ttttcgagtt gcttttgaaa ttgcaccgct tcggctttca gctcgctagg caatttttcg
     1741 gcagaagtgg ccaccggctt tgcagctgct ggcgcaggtg cagatccggc ggacgacttc
     1801 aatgcctttg acgcattccg catgaaagta ttgtaggcgg aatagctctt gaacccttgt
     1861 ttttcaacca tatttgtacc gattttgact gaaagatcat gccgagcttc agctaatttt
     1921 ttttccaaat cttcgatatc catttttgcc atgcgtaaac tcactcctta gaatctagtt
     1981 cgataatttc cgttagaaga agatcaaatt ggttgatcgt ttcttctgaa agtccaccgg
     2041 tgtattcaat aatagcgtca ctatgccttt ctaacagctt ttcaagctgt tcaacgtcaa
     2101 tcgtaactgt cttagccgtc atttttttgt tctccttttt cgtcataact atgaatcaaa
     2161 aagccgtcac gttcgccttt tgcatagttc attagttctt tcatagcttg atctagcgaa
     2221 gaatttttct ttgccatctt tttacgcagt ctttttttca actaaatcac tcctctattc
     2281 tttgctggtg tcattgatcg tgatttcata tccaacgact tttcgcccac gctttaaggt
     2341 tgtgacgaaa aatgaaactg acatcttatc agataattct tcaatagcac gagtaattac
     2401 tttttgaaaa aattgacctg caggaaggcg tttgtcttta ccaagaaacc aagattccca
     2461 ttcttccaaa tcacctgtaa tcgttgtgtg tttttctttt cctctacggt aggattccca
     2521 gagttttaac attatgagag catatttccc attgatccga gataattcac ctaaacgaaa
     2581 gctataaaac ttttctttga gatcaagaac gtatggagct gaatcagcag aaaatttgaa
     2641 tcttactaac ccgtcagcat aaaaatcaat ccaagaaaat aattgcgtca ttctgagacc
     2701 ttgagtacca tcggggcgct cgacacgaag ataaagcgca gtattttcat taagcttttt
     2761 gaaagcttca gctattcgac ggtaagaatc tccagaggtg ttcaacccaa aatgtttgat
     2821 aatttctaat gcggttacct cgtattcgtc atctagtttt gaatctttag ttacaaagct
     2881 aaaacagtag tctaaaactc gttgctcaaa cgatttaagc cctccaaatg ccttagctaa
     2941 gtcgtttcct tgcactacta aataatcttg acggcttagt aagtctttta atgcaatatc
     3001 agctttttta tttggttgtc ccaaactgaa aatcctcctt ttgtactttt tttaaaataa
     3061 taccatttat tcagtttttt tgcaatattt gaattttcaa cgtggaaaat agtatttttg
     3121 acgtggaaaa tagtattttt gacgtggaaa atagtatttt caacgtggaa aatagtattt
     3181 ataggcgcta caaacgctgg tataaagcca ttccttttcc cctaaagtat tttaaagtat
     3241 ttaaaagtaa tctataaagt attctcttta agcgcgcggg tgagaaaaaa atttaaattc
     3301 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
     3361 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
     3421 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
     3481 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
     3541 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
     3601 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
     3661 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
     3721 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
     3781 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
     3841 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
     3901 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
     3961 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
     4021 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
     4081 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
     4141 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
     4201 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
     4261 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     4321 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     4381 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     4441 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     4501 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     4561 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     4621 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     4681 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     4741 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     4801 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     4861 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     4921 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     4981 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     5041 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     5101 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     5161 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     5221 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgcc
//
