LOCUS       NZ_LR135432             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8927 plasmid 5.
ACCESSION   NZ_LR135432
VERSION     NZ_LR135432.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885924
            Assembly: GCF_900639635.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:11:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,819
            CDSs (total)                      :: 2,728
            Genes (coding)                    :: 2,644
            CDSs (with protein)               :: 2,644
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 24 of 84
            Pseudo Genes (incomplete)         :: 55 of 84
            Pseudo Genes (internal stop)      :: 23 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135432.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8927"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(42..662)
                     /locus_tag="EQB53_RS14600"
     CDS             complement(42..662)
                     /locus_tag="EQB53_RS14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(659..2785)
                     /locus_tag="EQB53_RS14605"
     CDS             complement(659..2785)
                     /locus_tag="EQB53_RS14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(2839..3213)
                     /locus_tag="EQB53_RS14610"
     CDS             complement(2839..3213)
                     /locus_tag="EQB53_RS14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3346..3807
                     /locus_tag="EQB53_RS14615"
     CDS             3346..3807
                     /locus_tag="EQB53_RS14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4119..4289)
                     /locus_tag="EQB53_RS14855"
     CDS             complement(4119..4289)
                     /locus_tag="EQB53_RS14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(4305..4844)
                     /locus_tag="EQB53_RS14620"
     CDS             complement(4305..4844)
                     /locus_tag="EQB53_RS14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4872..5609)
                     /locus_tag="EQB53_RS14625"
     CDS             complement(4872..5609)
                     /locus_tag="EQB53_RS14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            6254..6340
                     /locus_tag="EQB53_RS14630"
     CDS             6254..6340
                     /locus_tag="EQB53_RS14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6461..7150)
                     /locus_tag="EQB53_RS14635"
     CDS             complement(6461..7150)
                     /locus_tag="EQB53_RS14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(7152..7430)
                     /locus_tag="EQB53_RS15165"
     CDS             complement(7152..7430)
                     /locus_tag="EQB53_RS15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(7390..8304)
                     /locus_tag="EQB53_RS14645"
     CDS             complement(7390..8304)
                     /locus_tag="EQB53_RS14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8286..8666)
                     /gene="mobC"
                     /locus_tag="EQB53_RS14650"
     CDS             complement(8286..8666)
                     /gene="mobC"
                     /locus_tag="EQB53_RS14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(9108..9245)
                     /locus_tag="EQB53_RS14860"
     CDS             complement(9108..9245)
                     /locus_tag="EQB53_RS14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     BGC             1..8213
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4873..5609)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7394..8304)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtggtgcta gttttaaatc agctagcatc acgggtaaaa attataattc tataatatta
       61 gttccatagt ttagaacatt taagtcatgt gttactagta caacggcttt tttttctttt
      121 tctgttaaat ttttcagaaa gttcattata aaagttgagt tttctttatc taaatttcct
      181 gtaggttcat ctgcaaataa tatatcaaat ggttttaaca tatttctaat taatgctatt
      241 ctttgttgtt ctccaccgct taagagaaag attttttctt ttaagatatc ttttggcaaa
      301 gaaaaatctg ttaataaatt ataaatatga tcattggaaa tattactgtt aaactttttt
      361 gctaattcga aattgtattg tactgtttga ttttcaagaa gagcatagtt ttgaaataaa
      421 tagctaatat gatttcttcg tagacgttgt atatcatgtt tagaaacttt cttttcaaaa
      481 tagaataagt taccggtata ttgtttatct attaggctta aattatttaa cagagtagtt
      541 tttccactac cactttttcc tgttactata ataaaatctt tttctgatat tgaaaagttc
      601 caattttgtt tgatcttttt tttattaatt gacagggtga aattctcaac tgataatatc
      661 ataaaatatt ctccttaaaa tatttaaaac tatttttttt ataaataata atagaagtaa
      721 ttagatttat taaaaagatc ataaaaatta taaaatacaa gtaagtgttt tttgaaaata
      781 tcaattgaaa acacatagta gcaaataact ctattgtaaa agggagtaaa cataaatcta
      841 aaacttgact tattggtgaa tatccaaata ttttttttaa agctattttt tttgaattta
      901 tagtaaaact aacaaaaatc atttgaaaat taattattat aaataatcct aatattaaaa
      961 gatattgtag atatgcattt aatttattta cttttttttg agataataat tcaccagttt
     1021 cttgaaaaac agaataagaa aaatttatga tattttctag gttaaattct tttatatacg
     1081 gattaatttg attaaaaaag ttttggtttg aagtatgttt ctgatcaaaa aataatccac
     1141 catcactagt caatatattt ccccaaaata atgattctgt tatttctgga ttaataactc
     1201 taactatggg atcagttatt gtatagttat cttttgcctt tccatataag gtattaaaac
     1261 taaaatactt ttggttatta ttaacaaata ttagttttat ttttaaattt ttcaaattag
     1321 gatttttata aggagcttga ggaggagagt cctcaagtct aaactgtatg tcttctaaat
     1381 aatttttttc aatcattttt gcatattctt tatatttttt aggtactaga atgtaagtag
     1441 ttagtggatc agagtttatt aaatttaagt cagtagattc ttcaaaagaa ataggattgt
     1501 aatattttag atagtttaaa tcaacacata ttcctgcacc agcaggttga ctaacatagt
     1561 cttcaaaatt tggataaact ttttgtccaa taaaaaattc tttattattc tcttggatag
     1621 ttgcgtaatt gtatggcgcg ataataaaag ttttataatt cttctgtatt ttataaacaa
     1681 atttactagc ttttttcaag tagttatttt cttctacggt gttgtttcta tttaattgat
     1741 tagtaatatt tgttttgtat aaattagtag ttctttccca atatttaaga gaattatttt
     1801 cggcttcatt atgggtattt attacttttg tagaagctat taacagaagg atcatgctta
     1861 agaatagaca gatatttaat atacaaagat ttaacttaag tggtcgtgaa ttttttagat
     1921 aattttttat actagataaa tgatagatac ttagagtaac tatgataaat aaaaaactaa
     1981 ttataaaatg aaaaatacta catagaagaa atgataggaa aaaattttcg ttactgtaat
     2041 atatgctcaa aaaaatagat aaaatgaaag ttataatcaa agaacaaaat aagaggttta
     2101 actctttaat agaatctttt atagtatcaa aagagttata gcctaacatt ttttttagag
     2161 tgatttcttt catttttgaa aaagcaaata taattgtagt aattacaata aatatactaa
     2221 gatagcagat aacaagaaca atattagtat taatttgaaa agtatctgat tgattgatat
     2281 tggaaatagt aggtgttcca tattttttca gtacaggaat taatttgttt gagtttcctt
     2341 gtacatataa tatttcactt aatccaatat tttccacatg agaaaaagga taaatattta
     2401 tatttaaaaa attatttgga agttttaatt ggtacttaga atttttgtta ttagtataat
     2461 ggactttaga atcgtttctt gctaatatat tttttttaga aaatttccag tgtttgttag
     2521 cgttagggtt tgtagcaaga atagttagtt ggttaacagc agtaaaacta tattgagaaa
     2581 tattaatatt ttcttgtgta gatatttcat acaaagcgtt tatataactg ttaaagtcca
     2641 tatcatcttt atattgcata tgaatttcag cggtattttt atatagcgct tcagaagtct
     2701 ttataccatt tataacatta gtagaaaaaa atagtattgt aatgcagaca aaaagtgata
     2761 aaacagcgtt tatttttttt aacatatttt gaatttcctt tcaaatgtaa ataaaaaaga
     2821 gagtttaaac tctctttttt aataaacatt ccaccaacca gtatttccag taagtgtggc
     2881 attgcatgtt gcataagctc ttactccagc tttctgaact gatcgtactt gaccgcctcg
     2941 tccattttta gctgttgcac tatgtttagc tctagcatgg tagtaatttg cataaacttt
     3001 aaatccatta accccacagt tgaaatcccc gccgccagca gcatatctca atttactaga
     3061 agaaaatcct tcttctgtag cttccattcc tcctccgtaa atggcggtat ggtcttcggg
     3121 tacatctaga tttaaagctt ctgtggcact agctattcct ccactagtta ataagacacc
     3181 cattaaagca gaaagacata aaaatttttt cataacttat ttctcctatc ttttttacta
     3241 gaattatttt attttagtgt gttttataaa ataaagaact tttttggcgc gttagtattt
     3301 ttatgataaa attttatgta aataatcgtt tacataggag aaaaaatgga ggatttagga
     3361 tctaagctaa gagaattaag agtgaaaaaa gggtatactc aagaatatgt ggcaagaaaa
     3421 tttaacgtta cacgtcaaac tatttcaaag tgggaaaata acaaaacaac accagatata
     3481 gagacactaa aaaaaatttg tgaattttat agtactaaca taaattctat tttaaatgaa
     3541 gataattcaa aaaataaaaa aaataattta ctggtattga cagctattat ttttaatata
     3601 tttgttacta tattaggttt atttgaattg attcaaatga atttttttag ctttacacat
     3661 ggaatattta cttggtttgc tctatctgga ttaatagtaa tagatatctt aaattatttt
     3721 tatatttact attcttattt aaaaaatgaa aaattttttt atacaatgtt tttcccccta
     3781 gttattttga ttattttatt aagctaatat gaagtagtta gttatttaat tgcatagttg
     3841 acataatgta cactatgcca agtagcccct ctaccaatgg atagagacgc tcactttgtg
     3901 atttttaatt ggtctagcta aaagcaactt tctaaagctt gatttttcgc ggttatctat
     3961 atctaattct ttgtgcattt ttatctgtca tttttttaag acttgttatt ttttgtaaaa
     4021 tatggtaaat ttttggtagc gcttcataag cgcgtgcttt ttcaagaata aacactcatt
     4081 attttttaac tggctgctcg gaagagcagt cttttttatt atttatccgt ctcttttaca
     4141 aacaattctt cgttaggact gttgtaataa tttggattca atttcaattc accatgttta
     4201 ttccctttat tgattactga caataacatc ggactgaagc ctagaattcc tagtaaaata
     4261 gaaccgaata gaagattttt tcttgtcatg aatgttccct ccagttattt ccaaagttgc
     4321 caccattttt gcttcttctt ttttgtgtgt ttttctatcg attctgtttc ttcagaaaga
     4381 atagtagttg attcatcttc tggagtttct tccgtataag ctaattgtaa ctgttcttgc
     4441 agtttttcga tctgttgatt tgcctgtagg gtgagctgtt gttgctggtc gagtaatttt
     4501 tgcatattag aattattttg ctctttgagg gaaagttgtt ctcgtaaaaa ttcattttct
     4561 tcacgcaact tatctagcaa ctcgtttaca atcacgtttt tgcttgatat ataaacgttt
     4621 ttatcgtttt cttgttcata ctcatgcaac ctatatagta acctttggtt acctaagttg
     4681 ctagcaagtt gcaatactcc agaacgtaat acaaccttat aaccacgctc atttttagct
     4741 aataaggacg ggtctaattt atcaacatgt ttagagattg cttgcctact tatttcaaat
     4801 aattcagata attcctttat tgtaaaggtt tctttgggtt gcatgataat tgcctcctaa
     4861 attgttaggt attatttctc taaccaatta tctaaggtaa ttgaaggtat attttcttca
     4921 aaataaattt gcagagatcc tatcttattt cctttgtagg ctttaatttt tttgatttca
     4981 aaagttttaa aaatagcagg gtgagttgaa ttaacgttta aaaattcttt tttcgcttgg
     5041 gcaaaaattt gtttatcaat atctctcatg cgataacttt ctggtattgc taatagagct
     5101 ctaaaatctt ctatttttac aaaccagtga ccttttttag atcttctgtc tttatgagcc
     5161 ataagttggc gatataactc tttaacgtag ctactcttta agtttgtcat attttctaat
     5221 tcgaaacgag taaaattagt tccgattgaa ttaagtatat attcaaattc ttcagaaacc
     5281 tttattaata atgtttcatt cactttatcg attttaaatt tttgaaacag atttaattgt
     5341 tcatatgttt tttcgtcttc ataaataaaa gacaaagtaa tgattttttt tgacattttt
     5401 attaaatcat tagcaaaacg gtcgccacgt gcatttatat tgtaatcaat taattctttt
     5461 atttgtttaa atgtaaaaat ttcttctttt gtaccttttc ttttcatttt agaacatatt
     5521 ccccaaaata aatccatatc tataggggta aattttctta aagggacaga atttaatcca
     5581 ttattatatc gaactatatc gttactcaat aaaaatcacc tcaataaatt atgttatcat
     5641 agacataaga ggatgtaaaa cacaatttat cccccatagt gtgacaattt atcccccata
     5701 gtgtgacaat ttatccccca tagtgtgaca atttatcccc catagtgtga caatttatcc
     5761 cccactacct atgtcaatac ttactctccc ataaggttgc taatccctaa cagttattat
     5821 taacagtata tctaacagta tatctaacag tatatcttaa gagcacattg aatttcccgc
     5881 ctaaaaaact cctacttttt cctccaaaaa ggttcgcgaa tttttctaaa ctggaaaact
     5941 tgctccctac ggtcgctaaa aaggtgtgat cgctatgctc tcatattata ctgcgcagtc
     6001 gcctccggct cctttgctaa aaccgttgtt caaagaggag gtgtgagact ctgaaagctt
     6061 gtctaagccg ttctaagaca attcttgatt aatttccctt aatctagtcg agaagcgcct
     6121 tacaggcggt cagggcaagc cctaacaacc ctaaaacaag tgatacttaa aaacaccaca
     6181 caaatttaat tttttaagat ataataactt gtcgcctatc cgacagtctt gcgactggaa
     6241 ggaggtgtat ctaatgtttg aactgttcat ttgccccctt cttgtaggag tttgtgtctc
     6301 tttattcagt tattggttga ataaccgaaa caagaagtaa aatcaataaa ggcgacatca
     6361 acctgaaaaa aagccccaga gaatccgacc tctctggggc tttcgtgtat ctaatgtttg
     6421 atcatttgca cattcagttt aacatgattt taaatcaaat tcaattttgt tgttgctgat
     6481 ctaaccattc cttgacactt tttagatagt ttaaggcggt ttcttttttt gtcggatttt
     6541 gcatgttagt tgcttgttga agtaagattt catagcgttc atatgtgaca gattgttgtt
     6601 tttctttttt ttcttcttca ctggcaaacc aagggtttgt gtctccaaat agatcactac
     6661 aggcttcttc tatctttgta tcttttacat attgcacgtc ttttttggtt cgctcatagt
     6721 tgtcttgaac ctctttttgc tgtttttctg gcaagttttg ataaagacga gtgagttttt
     6781 ccatgttttt atccgtcaaa cgtttttctt gattcacaaa aatatcgttc atttcctcat
     6841 aacaagtggt aaactcttcc attttttgtg ctaatgcttg ctcttttttc tttttatcac
     6901 gcgctgattc atacttttga gtgacatttc ctaataaaat acttctagaa acgcgcttat
     6961 ttccctctaa aacgccgaat aagccgcttg aaacgattat caaggaaaat atacagccaa
     7021 gaaagaaaaa tcgcttaaat cgaattttag agccttttaa tgaatatctt gtttgaaaaa
     7081 ttttatagat gagccaaata cttcctgaaa tactaacaaa aatgagtaga tagggtaaat
     7141 aaaatgccat tttagcctcc ccatactaac caaaatgcga acagtgtcag gactaagcaa
     7201 cataaggggg caagaaaatc caatgatcgt tgaataagca cctttttctg ttccttattt
     7261 cgctggtcta atagctcttg atggagtttt ttaacttgta aatcaatcgt agtttgtatc
     7321 ctttcttgcg tataaggaac gagaaaagca cgttgtttct ttatttcgtt ttctatgtct
     7381 tttaacgttt tagagcgata attcttgttg gtggtctgtt cctctttcaa cgtgttgttc
     7441 atggtttctt cgagccggtc taatttccgg ttgttcaggc gtaagagttc gttgttctct
     7501 tcgtacttct tctgcagttt cgcgaatgct agctcgagct cttcgtagct cgttggttcg
     7561 ctgttggtta taggttcgtt ctcgtctgtc cagctcatgt aaaatcgtct ccttttcgta
     7621 gtcctttcct agtctgttgg atcgtacttt tttctttttg tggatcaatt cgtaagtgaa
     7681 atttttcccg cgattgaagc aatgaatccc caatgttttg agggaattac aaaattggtc
     7741 ataggttctg gtttttttgt ttttcattgt ttgatcgatc gcaatccgaa tttcgtcttt
     7801 ccaaacaggt tcattttttt cttttaattt tatttcagac atggttcgtt tttctggttt
     7861 ctttcctcgc tcagtgattg gtaagttatg tgcaagacaa atctcgtcat ttaggtcata
     7921 acatttgttt agaaatgctt gaccatgagc ttgaaatttt tttccagtct ccagattaac
     7981 ggagttaatg acaatgtgat tatgaatatg gtctttgtct gtatgtgtgt aaatagctat
     8041 ttggtgatcg ggggctattt tttttgcata ctctgcccct agttgattag ctttttcagc
     8101 ggtaatttct cctggtcgaa aagattgaat caataagtgt gcctgtacac ggtcgtcttt
     8161 tccatacagc atgcgaattt gtttcatttc acttttggca taatcgacgt cacagtttat
     8221 tcctgtttta atggttgctc gtggttctgc gtaattaatt gcacgactac aagaagtggt
     8281 tcggcttacc ttaattgttg ccatatcttt tgtagctcct tttgcatgat ttttaggttt
     8341 tcggataaac gttgagcgtc accagaagca acgtcatgtt ggtgtgtatt gcaccactta
     8401 gcaatttgat tgatattccc tccagctttg gctaaatcgc gcgcgattgc ctgggcaatg
     8461 tctggcgcca ctttcggtgt caccagacgc gcgttttgtg ccttggcttt cacgaaagcc
     8521 ggcacactca tttgcaaagc ttcagccgat tgcttcagct ttgcaaattc ggattcgctc
     8581 actcgaaaac tgatttgttt tggctcggat cgataggtat attttttaga agaattgcta
     8641 gccaaatttt gattttgttc gctcataagt cccacccttt cagtacgtcc tacggacgta
     8701 tcgttcgcaa taccattgta cctaaaaagg tacaatgagt aaagctcact aaaagggttt
     8761 ggcaagctct atatttgcta gccaaatacc tacgtattta gtgcaaataa ccgcttgtca
     8821 aaggggcgca agctccccct tgaatccccc tcaaaagcca cgctccacaa tacaaaaaaa
     8881 gctagcaaat gctagccact ttttgtaaag tttcgcttat ttcaccgaag atgaaattgt
     8941 tgctttgcaa caaaaaacat aaatgctttt ttgtgtaaac tactaactat ttagctaaaa
     9001 ctgatagttt tattaaacat tcagcaatat ttttagccaa ttttttatca gactttcggc
     9061 gagtcattat gataatatgt ccactttttg ttacttctac caaaaatcta gcaataactc
     9121 tcccattctt cagttgattt agtttcataa gtcttttgta attgacggtt taattttttt
     9181 ggcaattgca aagaataaat aagtctatca taaaattttt catactcttt tttcaataaa
     9241 atcacttggg tgcctccttt ttattaagtg attatttaaa ttttgggata ggagagatgg
     9301 aatataaaat aaatacc
//
