LOCUS       NZ_LR135431            11969 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8927 plasmid 4.
ACCESSION   NZ_LR135431
VERSION     NZ_LR135431.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885924
            Assembly: GCF_900639635.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:11:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,819
            CDSs (total)                      :: 2,728
            Genes (coding)                    :: 2,644
            CDSs (with protein)               :: 2,644
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 24 of 84
            Pseudo Genes (incomplete)         :: 55 of 84
            Pseudo Genes (internal stop)      :: 23 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8927"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            complement(58..396)
                     /locus_tag="EQB53_RS14525"
     CDS             complement(58..396)
                     /locus_tag="EQB53_RS14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176747811.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLKLPAEDNYSE
                     MLERMMDVLNSTINAGLAKSSVLTNEFVHRYLAQTTDEAEKEFWLKLMSHVMQLNQISV
                     INSKEKGM"
                     /name="hypothetical protein"
     gene            complement(467..700)
                     /locus_tag="EQB53_RS14530"
     CDS             complement(467..700)
                     /locus_tag="EQB53_RS14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            complement(697..1677)
                     /locus_tag="EQB53_RS14535"
     CDS             complement(697..1677)
                     /locus_tag="EQB53_RS14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127823940.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2124..3539)
                     /locus_tag="EQB53_RS14540"
     CDS             complement(2124..3539)
                     /locus_tag="EQB53_RS14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_127823943.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYISKQREKNERDK
                     ALMASERAKIEEEKKSIIDLEKELAVVFQKDPVPLTSFEKLPDGSYRLSSADFVDLYQK
                     ASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDLSAVVAENKK
                     LKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVS
                     QRKLERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(3761..4123)
                     /locus_tag="EQB53_RS14545"
     CDS             complement(3761..4123)
                     /locus_tag="EQB53_RS14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323570.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNKSQ
                     MSESKFTSMFSDVDLR"
                     /name="hypothetical protein"
     gene            complement(4114..4464)
                     /locus_tag="EQB53_RS14550"
     CDS             complement(4114..4464)
                     /locus_tag="EQB53_RS14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_197724077.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLKLPAEDNYSE
                     MLERMMDVLNSTINAGLAKSSVLTNEFVHRYLAQTTDEAEKEFWLKLMSHVMQLNQISV
                     INSKEKGCSLWG"
                     /name="hypothetical protein"
     gene            complement(4535..4768)
                     /locus_tag="EQB53_RS14555"
     CDS             complement(4535..4768)
                     /locus_tag="EQB53_RS14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            complement(4765..5739)
                     /locus_tag="EQB53_RS14560"
     CDS             complement(4765..5739)
                     /locus_tag="EQB53_RS14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_010727957.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(<6029..7374)
                     /locus_tag="EQB53_RS14565"
                     /pseudo=""
     CDS             complement(<6029..7374)
                     /locus_tag="EQB53_RS14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            complement(7596..7958)
                     /locus_tag="EQB53_RS14570"
     CDS             complement(7596..7958)
                     /locus_tag="EQB53_RS14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323570.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNKSQ
                     MSESKFTSMFSDVDLR"
                     /name="hypothetical protein"
     gene            complement(7962..8300)
                     /locus_tag="EQB53_RS14575"
     CDS             complement(7962..8300)
                     /locus_tag="EQB53_RS14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176747811.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLKLPAEDNYSE
                     MLERMMDVLNSTINAGLAKSSVLTNEFVHRYLAQTTDEAEKEFWLKLMSHVMQLNQISV
                     INSKEKGM"
                     /name="hypothetical protein"
     gene            complement(8371..8604)
                     /locus_tag="EQB53_RS14580"
     CDS             complement(8371..8604)
                     /locus_tag="EQB53_RS14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            complement(8601..9575)
                     /locus_tag="EQB53_RS14585"
     CDS             complement(8601..9575)
                     /locus_tag="EQB53_RS14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127823941.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(10024..11439)
                     /locus_tag="EQB53_RS14590"
     CDS             complement(10024..11439)
                     /locus_tag="EQB53_RS14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_025481041.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYISKQREKNEREK
                     ALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLTSFEKLPDGSYRLSSADFVDLYQK
                     ASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDLSAVVAENKK
                     LKEENRSLRQRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKTLASQQNAP
                     ERNIGRSENEMEW"
                     /name="plasmid recombination protein"
     gene            complement(11661..>11969)
                     /locus_tag="EQB53_RS14595"
     CDS             complement(11661..>11969)
                     /locus_tag="EQB53_RS14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127823942.1"
                     /translation="QAKLRQQKKRLIEKENAEMGKALRKHFGFESFDDFERWLSSISDD
                     QQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNKSQMSESKFTSMFSDVDLR"
                     /name="hypothetical protein"
     Typing          complement(698..1599)
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.231"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4766..5661)
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8602..9497)
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.32"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2128..3632)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.821"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6173..7467)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10028..11532)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.004"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcttttttc aatttttctt cttgtgcttt aatatctgct aaagtcatcc ccataaacta
       61 catccccttt tctttggaat taataacgga aatttggttt aactgcataa catgactcat
      121 taatttcaac caaaattctt tttcagcttc atcagttgtt tgcgctaaat acctatgaac
      181 aaattcatta gtcaaaactg aagattttgc taaaccggca ttgattgtgg aatttaaaac
      241 atccatcatt cgctcaagca tttcactgta attatcttca gccggcaatt tcaaataatc
      301 agctaaccgc tcatacatca attcaattct atcaactgtc tcttctctta aatcaatttt
      361 atccatgtta cacctcattt ccaaaactgc caccatttcc gattctttaa ttttcgatac
      421 acctaattca tcagcaagat tttttatggt catcttttct gacaaatcag tcattagctt
      481 ctccactact ttcagctacc gcataaatat atttaacggc gtgcttaggg ataataatcg
      541 gagagtcaat tttattattt ttcaaaatca attcatattg cgttacatca agtaacttac
      601 cggacatttt tttgctatcc aaaaaaacaa ccacaacgta atcacctgtt aaatttacca
      661 accaattttt cttaccaaaa tacttttgtt tttctgtcat ttttcttcct cctctgttac
      721 gtctagccct agccctagaa gccatgtgtc taattctttt tttaaatcgt ctgccgattc
      781 tgcaaaggtt tctcgtaaat gattcgtcaa ttcatcattt tgactttctt gcttcagcaa
      841 atttcgatat ttatattttg tctttcgatt aatttgcccc caataatcat gttctgttgt
      901 taaagcagta atcatcattt tgtcagctac acttgtatca gcaggcaagt aatgaggact
      961 agcaaaacta tccaagcttt cttgaaccat tgcatgccaa tcagtcgctt tccctcgtct
     1021 taattgtaat tctaaccgcc accaactcac gatttctttt ggcacaattt ttcttttctt
     1081 ctcttgttcc agttttttat tatacatccg aatttgacgt tcagaagagc gagatcccca
     1141 atatgccgtc tctaattttc cattccgtcc ataaataggc ttaaaagaaa ctggatcaac
     1201 tacccgatat tgtgtaataa actcatcagg tacatcgata atgtcgcaag caatatctgc
     1261 acgtgaaaag tgaggttcga taaacaaatc atgaataaag tttttcatgg aactagctaa
     1321 gaattgacta atcttattcg gattgaagtc tatacgccct ttcccctctt gccattttaa
     1381 aatttcaata taagcaatat tctcttcatg ttggtctaac aactgccaac cattattact
     1441 aacagatctt aaataaccgt taccttcatt taggcgcata agctgttcaa aatctaaaat
     1501 caacacatca ttttgagtgt gataataaat attttccttc agttttccaa cgatagtaat
     1561 tcggtcaatt gaccactgca tttttaaggg ggtgtacata ttttgtacat ttaaccgcct
     1621 gttagatagg gctgttaaat tttcccactc atttacgatt atgccatttt tactcaaatt
     1681 ttgttcctcc tcaaagaaaa agagacttct ggaaagaatt ttcaaaagtt cacgtaactc
     1741 ttaaaaattc attaccaaaa aatctctagt cttgaagaaa tctaaaaata ttcgtataat
     1801 gattaagaag ttacggagct agtgcgatta gtttccgtaa aggtcgagaa cgttggtagc
     1861 gttgctcgac tattttttta ctcttttttc agttcatttg tttgctaact caataatagc
     1921 acttctcttt agctatgcaa atatccaaaa aataaaaccg caccctttga aaagggcgcc
     1981 cccaaacttc gctaacgcta ggttggccaa aaaatggact ggccattttt tagcaaatgt
     2041 cagcttaata ggctagtggt ttagccgtca actccttatc ctcggcacaa accgcctccg
     2101 gtaccgttga ctgcacatcg taactaccac tcgatctcat cttcagaacg ctctaattta
     2161 cgctgtgaga cgttttgacg ataaaataag gttttctcct gctctaacac ttctaattcc
     2221 tcagaaagcc ccatatcctt ttctgcaaga ccaaaacgga tagacaaacg agtgatcgat
     2281 ttttgaaatg cgttttttaa tcgctctaaa cgctggcgta aagagcgatt ttcttctttc
     2341 agctttttat tttcagcaac aaccgctgac aaatcaacct tttgttgatt tttcaaaaaa
     2401 gaattttctt gttctaacgc catgttttta tcaaataaag cgatattttt atctaatgta
     2461 tttccaagcg attcattcgc catatcagca tattttttgc tgttcgtagc attagacgct
     2521 ttttgataca aatctacaaa atcagcacta gataagcgat acgagccatc cggtaatttt
     2581 tcaaaactcg ttaaaggaac agggtctttt tgaaaaacaa cagccagctc tttttccaaa
     2641 tcgataatcg atttcttctc ttcttcaatc ttcgctctct cagaagccat cagagcctta
     2701 tcacgctcgt ttttttctct ttgcttagaa atatacctca tttccgcaga aacgctctca
     2761 cgctctttta cagccgtttg gcgcaattca gaaatttcac tcatgatttc tttgtattcg
     2821 tgaatatctt taattttgtt ggtaccgaca atatcccttt tccaaccgta atcattcatt
     2881 aatcgttcta tagagtcaac ttcttgattt ctaaaattat tgaaaagcac tcgaccatta
     2941 tctttatcag cgtgtatccc ttgttgaacc agtgctttat taaagctcgg ctgcttttga
     3001 acaccccgtt tataaccttc agcaaccggc acaatattca agtgcaggtg cggacttatt
     3061 tcgtcattat gaataacagc attataaaca gccaaattag ggtttctctc ttgaaactcc
     3121 ttgtaatact gaaccaaaat tttattggca atcactcgat tttgttcagc ttgttgtttc
     3181 gatatgccag tagaagaacc atctgctcga tttgtctgga aatcatctaa aatgcctatt
     3241 tgcacaataa attctcgttg aggttccaac tttttatcct tgcgcacttt cgctaaatag
     3301 tcctcgattt ttctatcggc tcttttttgt ttcgcattat atttttctac cacttcgcca
     3361 aacaattcct catatttatc tgctagattc gtctgctcta aataaatatt ttcatccgat
     3421 ttcgtgaagt caatgtgctt atgccaatca ttggttttct ggcgatcagt taactcacga
     3481 ttattatgag aaatacttgt attcttcgtt ccttttttaa atgaaatact aaaccccaag
     3541 ttaaaaccct ccttcgcgct gtcgctaata aaaagagtgt aaacctttct gttactcttt
     3601 gtcaaagagt aacattttcc taattgtgac atggctgtca caattccaaa tgctctccga
     3661 gggtattcgc tgtcacaagt gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa
     3721 ctccttttac gctttatttt gcttcacgca aaataatcgc ttatcgtaaa tccacgtcac
     3781 taaacattga cgtaaattta ctctcactca tctgactttt gttattcggt atcacttcga
     3841 ataagccgtc agattcgttt agataatctc gttcgcttgg cgctgatttt tcatcagcaa
     3901 gccactcatt atccactgat ttttcgtgtt gctgatcgtc agaaattgac gataaccacc
     3961 gctcaaaatc atcaaaagat tcaaatccaa aatgcttccg aagtgcctta cccatttcag
     4021 cattttcctt ctcaatcaat cgttttttct gctgacgaag ttttgcctgt tcttttttca
     4081 atttttcttc ttgtgcttta atatctgcta aagtcatccc cataaactac atcccttttc
     4141 tttggaatta ataacggaaa tttggtttaa ctgcataaca tgactcatta atttcaacca
     4201 aaattctttt tcagcttcat cagttgtttg cgctaaatac ctatgaacaa attcattagt
     4261 caaaactgaa gattttgcta aaccggcatt gattgtggaa tttaaaacat ccatcattcg
     4321 ctcaagcatt tcactgtaat tatcttcagc cggcaatttc aaataatcag ctaaccgctc
     4381 atacatcaat tcaattctat caactgtctc ttctcttaaa tcaattttat ccatgttaca
     4441 cctcatttcc aaaactgcca ccatttccga ttctttaatt ttcgatacac ctaattcatc
     4501 agcaagattt tttatggtca tcttttctga caaatcagtc attagcttct ccactacttt
     4561 cagctaccgc ataaatatat ttaacggcgt gcttagggat aataatcgga gagtcaattt
     4621 tattattttt caaaatcaat tcatattgcg ttacatcaag taacttaccg gacatttttt
     4681 tgctatccaa aaaaacaacc acaacgtaat cacctgttaa atttaccaac caatttttct
     4741 taccaaaata cttttgtttt tctgtcattt ttcttcctcc tctgttacgt ctagccctag
     4801 aagccatgtg tctaattctt tttttaaatc gtctgccgat tctgcaaagg tttctcgtaa
     4861 atgattcgtc aattcatcat tttgactttc ttgcttcagc aaatttcgat atttatattt
     4921 tgtctttcga ttaatttgcc cccaataatc atgttctgtt gttaaagcag taatcatcat
     4981 tttgtcagct acacttgtat cagcaggcaa gtaatgagga ctagcaaaac tatccaagct
     5041 ttcttgaacc attgcatgcc aatcagtcgc tttccctcgt cttaattgta attctaaccg
     5101 ccaccaactc acgatttctt ttggcacaat ttttcttttc ttctcttgtt ccagtttttt
     5161 attatacatc cgaatttgac gttcagaaga gcgagatccc caatatgccg tctctaattt
     5221 tccattccgt ccataaatag gcttaaaaga aactggatca actacccgat attgtgtaat
     5281 aaactcatca ggtacatcga taatgtcgca agcaatatct gcacgtgaaa agtgaggttc
     5341 gataaacaaa tcatgaataa agtttttcat ggaactagct aagaattgac taatcttatt
     5401 cggattgaag tctatacgcc ctttcccctc ttgccatttt aaaatttcaa tataagcaat
     5461 attctcttca tgttggtcta acaactgcca accattatta ctaacagatc ttaaataacc
     5521 gttaccttca tttaggcgca taagctgttc aaaatctaaa atcaacacat catgtgtagt
     5581 gtgataatag atattctctt taagatttcc aacaatggta attcggtcga ttgaccactg
     5641 aatttttagg ggggtgtcca taaaagggac acttaaccgc ctgttagagg gggctgttaa
     5701 tttttcccac tcatttatga tcgtgccttt tttactcaaa tttagttcct cctcaaagaa
     5761 aaaagagatt ggtggaaaga attttccaga gttcacgtaa ctctaaaaaa ttcataacca
     5821 gcaatctcta gtcttgaaga aacctgaaat tattcgtata atgaatttga gttacggagc
     5881 tagtgtgatt agtttccgta aaggtcgaga acgtgtccag cgttgctcga ctttattttt
     5941 ttgctctttt ttcagtttat ttatttgata actcaataat agcacttctc tttagctatg
     6001 caaatatcca aaaacataaa accgcatgat ctaatatttt tagctcttca gatattccca
     6061 tatccttttc tgcaagacca aaacggatag acaaacgagt gatcgatttt tgaaatgcgt
     6121 tttttaatcg ctctaaacgc tggcgtaaag agcgattttc ttctttcagc tttttttgtt
     6181 ttcagtaaca accgctgaca aatcaacctt tttgttgatt tttttcaaaa aaaagaattt
     6241 tcttgttcta acgccatgtt ttttatcaaa taaagcgata tttttatcta atgtatttcc
     6301 aagcgattca ttcgccatat cagcatattt tttgctgttc gtagcattag acgctttttg
     6361 atacaaatct acaaaatcag cactagataa gcgatacgag ccatccggta atttttcaaa
     6421 actcgttaaa ggaacagggt ctttttgaaa aacaacagcc agctcttttt ccaaatcgat
     6481 aatcgatttc ttctcttctt caatcttcgc tctctcagaa gccatcagag ccttatcacg
     6541 ctcgttttct tctctttgcc tataaatata cctcaactca tcagaaacgc tctcacgctc
     6601 ttttacagcc gtttggcgca attcagaaat ttcactcatg atttctttgt attcgtgaat
     6661 atctttaatt ttgttggtac cgacaatatc ccttttccaa ccgtaatcat tcattaatcg
     6721 ttctatagag tcaacttctt gatttctaaa attattgaaa agcactcgac cattatcttt
     6781 atcagcgtgt atcccttgtt gaaccagtgc tttattaaag ctcggctgct tttgaacacc
     6841 ccgtttataa ccttcagcaa ccggcacaat attcaagtgc aggtgcggac ttatttcgtc
     6901 attatgaata acagcattat aaacagccaa attagggttt ctctcttgaa actccttgta
     6961 atactgaacc aaaattttat tggcaatcac tcgattttgt tcagcttgtt gtttcgatat
     7021 gccagtagaa gaaccatctg ctcgatttgt ctggaaatca tctaaaatgc ctatttgcac
     7081 aataaattct cgttgaggtt ccaacttttt atccttgcgc actttcgcta aatagtcctc
     7141 gatttttcta tcggctcttt tttgtttcgc attatatttt tctaccactt cgccaaacaa
     7201 ttcctcatat ttatctgcta gattcgtctg ctctaaataa atattttcat ccgatttcgt
     7261 gaagtcaatg tgcttatgcc aatcattggt tttctggcga tcagttaact cacgattatt
     7321 atgagaaata cttgtattct tcgttccttt tttaaatgaa atactaaacc ccaagttaaa
     7381 accctccttc gcgctgtcgc taataaaaag agtgtaaacc tttctgttac tctttgtcaa
     7441 agagtaacat tttcctaatt gtgacatggc tgtcacaatt ccaaatgctc tccgagggta
     7501 ttcgctgtca caagtgacaa cgaaaaaagg agccgaaagc gtgtcacgct ctcaactcct
     7561 tttacgcttt attttgcttc acgcaaaata atcgcttatc gtaaatccac gtcactaaac
     7621 attgacgtaa atttactctc actcatctga cttttgttat tcggtatcac ttcgaataag
     7681 ccgtcagatt cgtttagata atctcgttcg cttggcgctg atttttcatc agcaagccac
     7741 tcattatcca ctgatttttc gtgttgctga tcgtcagaaa ttgacgataa ccaccgctca
     7801 aaatcatcaa aagattcaaa tccaaaatgc ttccgaagtg ccttacccat ttcagcattt
     7861 tccttctcaa tcaatcgttt tttctgctga cgaagttttg cctgttcttt tttcaatttt
     7921 tcttcttgtg ctttaatatc tgctaaagtc atccccataa actacatccc cttttctttg
     7981 gaattaataa cggaaatttg gtttaactgc ataacatgac tcattaattt caaccaaaat
     8041 tctttttcag cttcatcagt tgtttgcgct aaatacctat gaacaaattc attagtcaaa
     8101 actgaagatt ttgctaaacc ggcattgatt gtggaattta aaacatccat cattcgctca
     8161 agcatttcac tgtaattatc ttcagccggc aatttcaaat aatcagctaa ccgctcatac
     8221 atcaattcaa ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc
     8281 atttccaaaa ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca
     8341 agatttttta tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc
     8401 taccgcataa atatatttaa cggcgtgctt agggataata atcggagagt caattttatt
     8461 atttttcaaa atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct
     8521 atccaaaaaa acaaccacaa cgtaatcacc tgttaaattt accaaccaat ttttcttacc
     8581 aaaatacttt tgtttttctg tcatttttct tcctcctctg ttacgtctag ccctagaagc
     8641 catgtgtcta attctttttt taaatcgtct gccgattctg caaaggtttc tcgtaaatga
     8701 ttcgtcaatt catcattttg actttcttgc ttcagcaaat ttcgatattt atattttgtc
     8761 tttcgattaa tttgccccca ataatcatgt tctgttgtta aagcagtaat catcattttg
     8821 tcagctacac ttgtatcagc aggcaagtaa tgagggctag caaaactatt taaactttca
     8881 tgaaccattg catgccaatc tgttgctttc ccacgcctta actgcaattc cagtcgccac
     8941 caagtttcta tttctggtgg cacaatttta cgcttggttt cctgttccaa ttttttgttg
     9001 tatagccgaa tctggcgttc gctggcacga ctgccccaat aagctgtttc cagcttccca
     9061 gaccgcccat aaattggctt gaaagaaaca ggatcaacca ctcgatattg tgtaataaac
     9121 tcatcaggca catcgataat atcgcaagca atatccgcac gtgagaaatg tggttctaga
     9181 aataaatcat gaatgaaatt tttcattgaa ctagctaaaa aatgaccaat cttattcggg
     9241 ttaaaatcaa tccgcccttt gccatcttgc catttcaaaa tctcaatata agcaatattt
     9301 tcttcgtatt ggtctaataa ttgccagccg ttattcccaa ccgcttttaa atagccattg
     9361 ccttcattca cccgcattaa ttgctcaaaa tctaaaatca acacatcatt ttgagtgtga
     9421 taatagatat tctctttaag atttccaaca atggtaattc ggtcgattga ccactgaatt
     9481 tttagggggg tgtccataaa agggacactt aaccgcctgt tagagggggc tgttaatttt
     9541 tcccactcat ttatgatcgt gcctttttta ctcaaattta gttcctcctc aaagaaaaaa
     9601 gagattggtg gaaagaattt tccagagttc acgtaactct aaaaaattca taaccagcaa
     9661 tctctagtct tgaagaaacc tgaaattatt cgtataatga atttgagtta cggagctagt
     9721 gtgattagtt tccgtaaagg tcgagaacgt gtccagcgtt gctcgacttt atttttttgc
     9781 tcttttttca gtttatttat ttgataactc aataatagca cttctcttta gctatgcaaa
     9841 tatccaaaaa cataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     9901 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg gattagtggt
     9961 ttagccgtca actccttatc ctcggcacag accgcctccg gtaccgttga ctgcaccgta
    10021 taactaccac tccatctcat tctcagaacg ccctatatta cgttctgggg cattctgctg
    10081 actagccaac gttttctctt gatctaatat ttttagctct tcagatattc ccatatcttt
    10141 ttctacaagc ccaaaacgaa ttgataaacg agtgattgat ttttgaaatg cgtttttcag
    10201 ttgagttaac cgctgacgca aagaacgatt ttcttccttc agctttttgt tttcagcaac
    10261 aaccgctgac aaatcaacct tttgttgatt tttcaaaaaa gaattttctt gttctaacgc
    10321 catgttttta tcaaataaag cgatattttt atctaatgta tttccaagcg attcattcgc
    10381 catatcagca tattttttgc tgttcgtagc attagacgct ttttgataca aatctacaaa
    10441 atcagcacta gataagcgat acgagccatc cggtaatttt tcaaaactcg ttaaaggaac
    10501 agggtctttt tgaaaaacaa cagccagctc tttttccaaa tcgataatcg attttttctc
    10561 ttcttcaatt ttcgccttct cagaagccac cagagccttc tcacgctcgt ttttttctct
    10621 ttgcttagaa atatacctca tttccgcaga aacgctctca cgctctttta cagccgtttg
    10681 gcgcaattca gaaatttcac tcatgatttc tttgtattcg tgaatatctt taattttgtt
    10741 ggtaccgaca atatcccttt tccaaccgta atcattcatt aatcgttcta tagagtcaac
    10801 ttcttgattt ctaaaattat tgaaaagcac tcgaccatta tctttatcag cgtgtatccc
    10861 ttgttgaacc agtgctttat taaagctcgg ctgcttttga acaccccgtt tataaccttc
    10921 agcaaccggc acaatattca agtgcaggtg cggacttatt tcgtcattat gaataacagc
    10981 attataaaca gccaaattag ggtttctctc ttgaaactcc ttgtaatact gaaccaaaat
    11041 tttattggca atcactcgat tttgttcagc ttgttgtttc gatatgccag tagaagaacc
    11101 atctgctcga tttgtctgga aatcatctaa aatgcctatt tgcacaataa attctcgttg
    11161 aggttccaac tttttatcct tgcgcacttt cgctaaatag tcctcgattt ttctatcggc
    11221 tcttttttgt ttcgcattat atttttctac cacttcgcca aacaattcct catatttatc
    11281 tgctagattc gtctgctcta aataaatatt ttcatccgat ttcgtgaagt caatgtgctt
    11341 atgccaatca ttggttttct ggcgatcagt taactcacga ttattatgag aaatacttgt
    11401 attcttcgtt ccttttttaa atgaaatact aaaccccaag ttaaaaccct ccttcgcgct
    11461 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
    11521 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
    11581 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
    11641 gcttcacgca aaataatcgc ttatcgtaaa tccacgtcac taaacattga cgtaaattta
    11701 ctctcactca tctgactttt gttattcggt atcacttcga ataagccgtc agattcgttt
    11761 agataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
    11821 ttttcgtgtt gctgatcgtc agaaattgac gataaccacc gctcaaaatc atcaaaagat
    11881 tcaaatccaa aatgcttccg aagtgcctta cccatttcag cattttcctt ctcaatcaat
    11941 cgttttttct gctgacgaag ttttgcctg
//
