LOCUS       NZ_LR135427             2056 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Enterococcus faecium isolate E8440 plasmid 9.
ACCESSION   NZ_LR135427
VERSION     NZ_LR135427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885812
            Assembly: GCF_900639645.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900639645.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,282
            CDSs (total)                      :: 3,190
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 34 of 109
            Pseudo Genes (incomplete)         :: 66 of 109
            Pseudo Genes (internal stop)      :: 28 of 109
            Pseudo Genes (multiple problems)  :: 15 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135427.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8440"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            complement(209..466)
                     /locus_tag="FQ475_RS16320"
     CDS             complement(209..466)
                     /locus_tag="FQ475_RS16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002377879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(467..688)
                     /locus_tag="FQ475_RS16325"
     CDS             complement(467..688)
                     /locus_tag="FQ475_RS16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            1305..1460
                     /locus_tag="FQ475_RS16330"
     CDS             1305..1460
                     /locus_tag="FQ475_RS16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1527..>2056
                     /locus_tag="FQ475_RS16335"
     CDS             1527..>2056
                     /locus_tag="FQ475_RS16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_284166694.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSA"
                     /name="replication protein"
     Typing          complement(132..307)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          1528..2056
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttatattatc gggcaatata taaaatcgaa tcgtcatggt ggaagtgtaa attcaaaaat
       61 aaataaagga taagagctgt taatttcagc tctttttttg tgcgtgaaag tcttagtcac
      121 tatgaccaaa actacggacg aaactatgac caaaagtagg tactatatta tgaccgaaaa
      181 taggtccaaa atggattttt ttgtgatatc aaaaaatata aggttgtttt gtaagaaaac
      241 cagttggcac agccgactta gccgccttcc tgtaggaagg tgctatcagt tttcccttat
      301 gctctttttg ttcttcagag tttaattcca gaatatttgt gagaagtagg tcaaagaggt
      361 cggtggtttc ttcgactttt ccgccggtca attcgataaa atcattggaa tatgattcta
      421 gcaatttttc tagttgttca acgttgatag ttgtttcttt aggcatttac tcaccttctt
      481 tggtggttat gactttttct agttttttaa gggcttctgg gctgaaaaga tagtttcttc
      541 taccggcaga gcgatattct tttccttcag tcagatgttc tttcgctaag cgcaaaacgt
      601 aagcttggtt gtaatcaagt tcttgtgcaa gttcgctggt cgtcataatt tcggccaaag
      661 tgaatttttc tgattttatc actctcattt atgctccttc catttaacgt ccgtctatat
      721 tacgtgagta cgtcggactt acgacataag cttataatat aagttttctg tgttgtggtc
      781 aattttatat tatcgaaatg actagtagaa aaaatcaatg ctctaaaaag ggtctatttt
      841 gcgttttaag cgatttagcg tctatttcgt ttagttatcg gcgcaatcgt taaaacacgt
      901 aagagaagcg agaatggagc ctgtaaggcg actggtagcg aaacggtcgt gttagattgc
      961 aaaagccgat agctgaacga aatagtaagc gtggcgcctg catatatttc ggtagaagga
     1021 ggtcggggtt cggggcggag ccctgaggtt tgttttttgg ttttggtgtt tatgaattgg
     1081 gcggaggagg gggggactac gacacccccc ttattggtca ttggtcattg gtcgatagct
     1141 gtttagcctt actctcccaa gggtttcagc gttttttttt gacaaaatca gtaatgccaa
     1201 gggtttaagg cctattattc gctaattagt atctaaactg ttgcacctta gttacttttt
     1261 tgttactatg gtttttaaga aaaagtcgtt ggaggatacg aattatgatc agtaatgaaa
     1321 aaaagagagt cgttgtaacg cttactaagg aagtggcaga taatcttgat aaaattgcga
     1381 aacaaatggg tcttagtaag tctggtctta ttacaatttg ggttaatgct aacggaaagg
     1441 aaaatgagca aaaaaaatag caaaagcatt agtgttggcg cactgctttt gctgttagac
     1501 tgttgttcaa ttaaggtgat tatagcatga cgagtagaaa aactaaagat gaacgcacaa
     1561 ggaattggac gtttgtagtc tatcctgaat cggctcctga aaattggagg gagtttttag
     1621 atgagttaca tgttccttgg gttgagagtc cacttcatga taaagatgtg aatcctgacg
     1681 gtgaattgaa aaagcctcat tggcatgttt tgataatgtt tagcggtaaa aagacgtatg
     1741 atcaaatacg tgagataact caaaagattc ggtctccaaa cccgcaaaaa tgtgctaatg
     1801 caaaaggtat ggtgcgatat tttgctcata tggataatcc agaaaaattt caatatgcta
     1861 aaagtgatat tatcgctcat ggtggtgcag aaattgctag ttatttgtca gtaacgagtg
     1921 cagaacgata tgagttgatt agagagatga tgtcgtttgt tgattctaag aatatcactg
     1981 aaatcaaaga tttaattgac tatgcaatgt cagaaagatt tgatgattgg tttccgcttc
     2041 tttgtgataa ttcagc
//
