LOCUS       NZ_LR135426             2947 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Enterococcus faecium isolate E8440 plasmid 8.
ACCESSION   NZ_LR135426
VERSION     NZ_LR135426.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885812
            Assembly: GCF_900639645.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900639645.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,282
            CDSs (total)                      :: 3,190
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 34 of 109
            Pseudo Genes (incomplete)         :: 66 of 109
            Pseudo Genes (internal stop)      :: 28 of 109
            Pseudo Genes (multiple problems)  :: 15 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135426.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8440"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(134..550)
                     /locus_tag="FQ475_RS16300"
     CDS             complement(134..550)
                     /locus_tag="FQ475_RS16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(552..833)
                     /locus_tag="FQ475_RS16305"
     CDS             complement(552..833)
                     /locus_tag="FQ475_RS16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     gene            1633..2607
                     /locus_tag="FQ475_RS16310"
     CDS             1633..2607
                     /locus_tag="FQ475_RS16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2604..2744
                     /locus_tag="FQ475_RS16315"
     CDS             2604..2744
                     /locus_tag="FQ475_RS16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     Typing          1658..2604
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctttttttct gttatttctt ctgaaattcg tttttttcat tcaattggtt actatcttat
       61 tgtaaatgtt gctattaaga tggtgatcct acccaaaaaa taaaccagga atttaaatct
      121 cctggtttat tttttatctt tttagaaaat ttgttgggtc gaaattttta gaagtaaatg
      181 tttgattggt ttctttttga cgattttttg gttgatttcg tttggaggga ttaagttgtt
      241 tttcaatttt ttctttcgtt tcttgataca ctttagaatt tgtgccaata agaatacctg
      301 tatcttttag atataaatca gcaaattctg caatgatttc ggacatgatg gaaatttctt
      361 ttcccatagc atttagtttg gcttgattgc tggtagtagt cgtgttggta tcaggttcat
      421 aactcataca taaaaaacga atggcttccg agtaattgtt gatatctgga tttctttcga
      481 taatggaatc taaaacgtcc acgtgactgg gataaagggt aacgtgtttt tgaacagaag
      541 tttttctcat gttatcttcc tccgataagt gtttcaatga gttggttgtt tgtacgaaca
      601 tattcattca gcgtttcagt atttttttct tgaatagcga taaatttttc gagtaataaa
      661 tcgaattgat ctttcttgta atgcataagt gcgtggtcaa aatcgttttc gattaggact
      721 tttaagtagc gatttcgact ccattttttg ccagttttat ttgtgagttc ttggcaacgt
      781 ttgtctattt ttgcaataac ctttgaatcg atcttccgaa ctaaaatgtc catagaaaaa
      841 ctcctttcgc atttgtgtat aatttatgta tatcatacga atatagtatt tgtatagctg
      901 ttttttgcta aaatactata cataagctag ccacgatggc gaaaaagtga tatcaaattt
      961 ttttctgaga aacgtgatag gcacagccta tctacccact ttttttagaa agtgggtaca
     1021 cgtttcctta tgctctttat aaaatggtga tgtgcgactt ttttctgttc gtaaaaataa
     1081 aaaaagttta cgaaaatgaa actaaactga acgattatga cgggcatgta atcaagatga
     1141 aatgatttta taagcgcgta ttttgcgttc taagacgttt tagcgttcat ttcgtttagt
     1201 tatcggcaca atcgttaaaa cacgtaagag aagcgagaat ggagcctgta aggcgactgg
     1261 tagcgaaacg gcgtgttaga ttgtgcaagc cgagaactga atgaaatgaa gagcggaagc
     1321 gtgaaataat ttcggcagga ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc
     1381 tttttatttt tttaagagga gtgctataat caaagaaaac gagaaaaaag agcaaaataa
     1441 aagccgaacg gtgttggaag cacccttcgg ctaattacgg aaactactca cctagttccg
     1501 taactcaaat tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1561 ggatttttag agttacgtga actctggaaa ttctttccaa aaatctcttt tttctttgag
     1621 gaggaacaaa atttgagtaa acgtggcacg atcataaatg agtgggaaaa acttaaccgc
     1681 atatctaaca ggcggttaaa tgcacaaact atgtgcaccc ccttaaaaat gcaatggtca
     1741 attgaccgaa ttaccattgt tggtacatta agggaaaata tttattatca cactccccat
     1801 gatgtgttga ttttagattt tgaacagctt atgcgtctaa acgaaggtaa cggatattta
     1861 aaagcggttg gaaataatgg ctggcaatta ctagaccaac atgaggaaaa tatagcttat
     1921 attgagattc ttaagtttca agagggaaaa gggcggattg actttaaccc caataaaatt
     1981 agccaatttc tggcgggctc gatgaagaac tttatccatg atttattttt agaaccacat
     2041 ttctcacgtg cagatattgc ttgtgacatt attgacgtgc cagatgattt cattacccaa
     2101 tatcgggtgg ttgatcctgt ttctttcaaa ccaatttacg gtcggtctgg gaaactggaa
     2161 acagcatatt ggggaagtcg tgctagtgaa cgacagattc ggatgtataa caaaaaattg
     2221 gaacaagaaa gaaaacggaa agttgttccg aaagaaatcg atacttggtg gcgactagaa
     2281 ttgcaattgc gaagggggaa agcaactgat tggcatgcaa tggttcatga aagtttagat
     2341 agctttgcaa gtcctcacta tttgccagaa gatgtgaaag taactgacag aatgatgatt
     2401 actgctctta taacggaaca tagttactgg gggcaaattc atagaaattc aaaatataaa
     2461 tatcgtgaat tgttaaagca agaaagtcaa aatgatgagt tgacgaatca tttgcgagaa
     2521 acattttcgg agtcggcaga tgatttgaaa cgagaattag atacatggct tcaaggactt
     2581 gatgtgacgg aagtaggtgc tgaatgagtg attcgataga aaaaatgacc ctaaaaaaac
     2641 ttgctgatga attgggtgta tcgaaaaata cggtgcgcaa tcaactggtc aattcgggat
     2701 ttgaccttgc tcagtataaa gaaaaaaatg cagtggtcaa ttgaccgaaa tttatgtctt
     2761 tgggttttgg ggttttaaaa cgaaaaaaag ggggcgattt tcgccccctt tgctctatgt
     2821 gaaaaatttg ttatacttat tttatcctta aaacatacca atctttttct tatttagctg
     2881 ctcgtacgag cagctttttt tacgattatg aattcaaatc atgtatttat aatttttttg
     2941 tttttat
//
