LOCUS       NZ_LR135425             3129 bp    DNA     linear   CON 01-JUN-2024
DEFINITION  Enterococcus faecium isolate E8440 plasmid 7.
ACCESSION   NZ_LR135425
VERSION     NZ_LR135425.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885812
            Assembly: GCF_900639645.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900639645.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 05:45:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,282
            CDSs (total)                      :: 3,190
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 34 of 109
            Pseudo Genes (incomplete)         :: 66 of 109
            Pseudo Genes (internal stop)      :: 28 of 109
            Pseudo Genes (multiple problems)  :: 15 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135425.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8440"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            complement(133..549)
                     /locus_tag="FQ475_RS16275"
     CDS             complement(133..549)
                     /locus_tag="FQ475_RS16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806812.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNQPKNHQKGTNKTVTSSNFDPTKFLKR"
                     /name="hypothetical protein"
     gene            complement(552..836)
                     /locus_tag="FQ475_RS16280"
     CDS             complement(552..836)
                     /locus_tag="FQ475_RS16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1203..1427
                     /locus_tag="FQ475_RS16285"
     CDS             1203..1427
                     /locus_tag="FQ475_RS16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225471215.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLKYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1551..1730
                     /locus_tag="FQ475_RS16290"
     CDS             1551..1730
                     /locus_tag="FQ475_RS16290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322661.1"
                     /translation="MEKDIYKQLTRQIQKEKEKGLLDHLDELELKADEINKNRMSAKEK
                     GIKLKDSSLQSDKS"
                     /name="hypothetical protein"
     gene            2452..>3129
                     /locus_tag="FQ475_RS16295"
     CDS             2452..>3129
                     /locus_tag="FQ475_RS16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_284166687.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMDE
                     KTNKKDWTSSDVIRNGKHYVKPHYHVLYIARNPVTIESVRKKIKRKLGNNSVAHLEIID
                     HIKGSYEYLTHESKDAKEKNKHVYDKKDILHINDFDIDRYVTLDESQKKELRNQLFDLV
                     NHCGIVNMKELAAFLDWRGQEYGITNQRDVVDVIASNSNLFRMMFDANYQNGFRPQYAK
                     RIDS"
                     /name="replication protein"
ORIGIN
        1 gaaactggag aaatgattga aggcgtgaat aaacacgtcg aataaatatc gaaaacttcc
       61 gttaactgct tggaatgaaa aaatagaata ttgggaaata aaaaacagat aggaataact
      121 cctatctgtt ttttatcgtt ttagaaattt agttgggtcg aaattgctag aagtgactgt
      181 tttgtttgtt cctttttgat gattctttgg ttgatttcga tttgatggat ttagtttagc
      241 ttcgattttt tctttcactt cttgatatac tttagaattt gtgccgataa gaacgcctgt
      301 atcttttaga tataaatcag cgaattctgc gatgatttca gtcatgatgg aaatttcttt
      361 tcccatagca tttagttttg cctgattgct ggtagtggtc gtgttggtat cgggttcata
      421 actcatacat aaaaagcgaa tggcttccga gtagttattg atatctggat ttttttcgat
      481 gatggaatct aaaacgtcaa catgactagg gtacaaagta acatgttttt ggacagaagt
      541 ttttctcatg gttatatcat tcctccgata agcatttcaa tgatttgatt atttgtgtag
      601 acatactcgc ttaatatttg agtattttct gtttggatag caataaattt atcgagcaat
      661 aaatcgaatt ggtctttttt gtaagtcgta agttgacggt caaaatcact ttcgattaaa
      721 acttttagat atttatttcg actccatttt tctcctgttt tattcgtgag ttcttggcaa
      781 cgtttgtcta ttgttgcaac aaactttgga tcaatattcc gaactaaaat gtccataaaa
      841 aatctccttt tgcaattatg tataatttat gtataccata caaatactat atttgtatag
      901 ctgttttttg ccaaaatact atacataagc tatccacgat ggcgaaaaag tgatatcaaa
      961 tttttttcaa agaaacgtga taggcacagc ctatctagca cctttcgtaa gaaagtgata
     1021 ccacgtttcc ttatgctctt taaaattcgg gggggagtgc tgcacgtgtg atcgataacc
     1081 aaatttaaaa atgcacgatc aaaaatttat ttagtttttc aaacgacatt tttttttgag
     1141 agtatagacg gcaaagaagt gattttattt ctagaaaaat tgtgagactg gagaaagaaa
     1201 aaatggaaaa atttccttac gaatattttc aaaacttaga aaaattagag caagcaattc
     1261 aagcaatcaa gttcgattta acagacgaaa aattgttgaa atataaaaat atgacgcccg
     1321 aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc ggaatttcag
     1381 acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg ggtttaaatg
     1441 gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac caaaaactaa
     1501 ataaaacgtc ttaaatcgaa taataatagc ttttaaggag gcgatggggg atggaaaaag
     1561 atatttataa acaattgacc cgacaaattc aaaaggaaaa agaaaaaggc ttactagatc
     1621 atttagatga attagaacta aaagctgatg aaatcaataa aaacagaatg tcagcaaaag
     1681 aaaaaggtat aaaacttaaa gattcgtcgt tgcaaagcga taaaagctag ataacgtgcg
     1741 gtcaacggta aatcgaatta gagacgctta gagaggctaa taagccctct tagcgtctct
     1801 ttgaggcttt aaggagttga cggctataaa caaaacccaa aagctggaaa cacaagaagg
     1861 tgaccatagg gaacgttctt gacgccttaa cggcgagtgg aaagtttttg gggtgccttt
     1921 tttcaaaaag gtacggtctt gatttgccga gcgatagcgc tggaaaattt ttggaaaaaa
     1981 tttggaattt ggaaaatctg gggggggggc tactacgacc cccccccata gtgtgccgag
     2041 tgccaatttt tgtgccaaaa agtgctttat ccttactgta ccaatggata gagccttttt
     2101 ttttcgaccg ccatttgacc gccgttttga ccgccgtttt gaaaaataga aaaaaatatt
     2161 gacaatacag ttgtcaaaag tttaccatga tattaacaat taactaaagg ggctgaaagg
     2221 catggcggaa tcagaaagac tggtagtaat tttaccgagt gaaattaagc gaaaacttga
     2281 ggaaaaagca aaagacaaaa gtttgagtat gtcgacatat gttaaattgc tagtaacaga
     2341 agacttgaaa aaatcagaga aaggaaattg agcgcaaaaa aagagcagaa acatatgtgt
     2401 tggcgcaccg tttccgctta actgatagtg ttcaattgag gtaagtatat catgaataag
     2461 aatataacga aagctagaca ttttggtttt ttactatatc ccgaatctat tccagagaat
     2521 tggagagaac agcttgagag cttaggagta tcaatggcgg ttagtccgtt acacgatatg
     2581 gacgaaaaaa caaataaaaa ggattggact agtagtgatg ttatcagaaa cggtaagcat
     2641 tatgtaaagc cacattatca tgtcctttat attgctcgca atcctgtaac aatcgagagc
     2701 gtaagaaaga aaatcaagag aaaattggga aataattcgg ttgctcactt agaaataatt
     2761 gaccatatca aaggctcgta tgagtatctt acgcatgaat caaaagatgc aaaagaaaag
     2821 aataaacatg tatacgataa aaaagatatc ttgcatatta atgattttga tattgatcgt
     2881 tatgtaactt tggacgagag tcaaaagaaa gagttgagaa accaattgtt tgatcttgtt
     2941 aatcattgtg gaatagtcaa tatgaaagag ttagctgcat ttcttgactg gagaggtcaa
     3001 gagtatggaa tcactaatca aagagatgtt gttgatgtca tagcttcaaa cagcaatctt
     3061 ttccgtatga tgtttgatgc taattaccag aacggatttc ggccgcaata tgcaaaaagg
     3121 atagattct
//
