LOCUS       NZ_LR135418             2947 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8328 plasmid 5.
ACCESSION   NZ_LR135418
VERSION     NZ_LR135418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885722
            Assembly: GCF_900639615.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,157
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 69 of 103
            Pseudo Genes (internal stop)      :: 21 of 103
            Pseudo Genes (multiple problems)  :: 14 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135418.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8328"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            <1..244
                     /locus_tag="EQB48_RS16950"
     CDS             <1..244
                     /locus_tag="EQB48_RS16950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127822575.1"
                     /translation="ILLFFLVFFDYSTPLKKIKRPQGISFPEPLEPPPAEIISRFRSSF
                     HSVLGLHNLTRRFATSRLTGSILASLTCFNDCADN"
                     /name="hypothetical protein"
     gene            607..888
                     /locus_tag="EQB48_RS16555"
     CDS             607..888
                     /locus_tag="EQB48_RS16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     gene            890..1306
                     /locus_tag="EQB48_RS16560"
     CDS             890..1306
                     /locus_tag="EQB48_RS16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1643..1783)
                     /locus_tag="EQB48_RS16565"
     CDS             complement(1643..1783)
                     /locus_tag="EQB48_RS16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(1780..2754)
                     /locus_tag="EQB48_RS16570"
     CDS             complement(1780..2754)
                     /locus_tag="EQB48_RS16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(1784..2730)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tattttgctc ttttttctcg ttttctttga ttatagcact cctcttaaaa aaataaaaag
       61 acctcaggga atttcattcc ccgaacccct tgagcctcct cctgccgaaa ttatttcacg
      121 cttccgctct tcatttcatt cagttctcgg cttgcacaat ctaacacgcc gtttcgctac
      181 cagtcgcctt acaggctcca ttctcgcttc tcttacgtgt tttaacgatt gtgccgataa
      241 ctaaacgaaa tgaacgctaa aacgtcttag aacgcaaaat acgcgcttat aaaatcattt
      301 catcttgatt acatgcccgt cataatcgtt cagtttagtt tcattttcgt aaactttttt
      361 tatttttacg aacagaaaaa agtcgcacat caccatttta taaagagcat aaggaaacgt
      421 gtacccactt tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa
      481 aatttgatat cactttttcg ccatcgtggc tagcttatgt atagtatttt agcaaaaaac
      541 agctatacaa atactatatt cgtatgatat acataaatta tacacaaatg cgaaaggagt
      601 ttttctatgg acattttagt tcggaagatc gattcaaagg ttattgcaaa aatagacaaa
      661 cgttgccaag aactcacaaa taaaactggc aaaaaatgga gtcgaaatcg ctacttaaaa
      721 gtcctaatcg aaaacgattt tgaccacgca cttatgcatt acaagaaaga tcaattcgat
      781 ttattactcg aaaaatttat cgctattcaa gaaaaaaata ctgaaacgct gaatgaatat
      841 gttcgtacaa acaaccaact cattgaaaca cttatcggag gaagataaca tgagaaaaac
      901 ttctgttcaa aaacacgtta ccctttatcc cagtcacgtg gacgttttag attccattat
      961 cgaaagaaat ccagatatca acaattactc ggaagccatt cgttttttat gtatgagtta
     1021 tgaacctgat accaacacga ctactaccag caatcaagcc aaactaaatg ctatgggaaa
     1081 agaaatttcc atcatgtccg aaatcattgc agaatttgct gatttatatc taaaagatac
     1141 aggtattctt attggcacaa attctaaagt gtatcaagaa acgaaagaaa aaattgaaaa
     1201 acaacttaat ccctccaaac gaaatcaacc aaaaaatcgt caaaaagaaa ccaatcaaac
     1261 atttacttct aaaaatttcg acccaacaaa ttttctaaaa agataaaaaa taaaccagga
     1321 gatttaaatt cctggtttat tttttgggta ggatcaccat cttaatagca acatttacaa
     1381 taagatagta accaattgaa tgaaaaaaac gaatttcaga agaaataaca gaaaaaaaga
     1441 taaaaacaaa aaaattataa atacatgatt tgaattcata atcgtaaaaa aagctgctcg
     1501 tacgagcagc taaataagaa aaagattggt atgttttaag gataaaataa gtataacaaa
     1561 tttttcacat agagcaaagg gggcgaaaat cgcccccttt ttttcgtttt aaaaccccaa
     1621 aacccaaaga cataaatttc ggtcaattga ccactgcatt tttttcttta tactgagcaa
     1681 ggtcaaatcc cgaattgacc agttgattgc gcaccgtatt tttcgataca cccaattcat
     1741 cagcaagttt ttttagggtc attttttcta tcgaatcact cattcagcac ctacttccgt
     1801 cacatcaagt ccttgaagcc atgtatctaa ttctcgtttc aaatcatctg ccgactccga
     1861 aaatgtttct cgcaaatgat tcgtcaactc atcattttga ctttcttgct ttaacaattc
     1921 acgatattta tattttgaat ttctatgaat ttgcccccag taactatgtt ccgttataag
     1981 agcagtaatc atcattctgt cagttacttt cacatcttct ggcaaatagt gaggacttgc
     2041 aaagctatct aaactttcat gaaccattgc atgccaatca gttgctttcc cccttcgcaa
     2101 ttgcaattct agtcgccacc aagtatcgat ttctttcgga acaactttcc gttttctttc
     2161 ttgttccaat tttttgttat acatccgaat ctgtcgttca ctagcacgac ttccccaata
     2221 tgctgtttcc agtttcccag accgaccgta aattggtttg aaagaaacag gatcaaccac
     2281 ccgatattgg gtaatgaaat catctggcac gtcaataatg tcacaagcaa tatctgcacg
     2341 tgagaaatgt ggttctaaaa ataaatcatg gataaagttc ttcatcgagc ccgccagaaa
     2401 ttggctaatt ttattggggt taaagtcaat ccgccctttt ccctcttgaa acttaagaat
     2461 ctcaatataa gctatatttt cctcatgttg gtctagtaat tgccagccat tatttccaac
     2521 cgcttttaaa tatccgttac cttcgtttag acgcataagc tgttcaaaat ctaaaatcaa
     2581 cacatcatgg ggagtgtgat aataaatatt ttcccttaat gtaccaacaa tggtaattcg
     2641 gtcaattgac cattgcattt ttaagggggt gcacatagtt tgtgcattta accgcctgtt
     2701 agatatgcgg ttaagttttt cccactcatt tatgatcgtg ccacgtttac tcaaattttg
     2761 ttcctcctca aagaaaaaag agatttttgg aaagaatttc cagagttcac gtaactctaa
     2821 aaatccatat ccaaaaatct ctagcttgaa gaaacctaaa aatattcgta taatgaattt
     2881 gagttacgga actaggtgag tagtttccgt aattagccga agggtgcttc caacaccgtt
     2941 cggcttt
//
