LOCUS       NZ_LR135417             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8328 plasmid 4.
ACCESSION   NZ_LR135417
VERSION     NZ_LR135417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885722
            Assembly: GCF_900639615.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,157
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 69 of 103
            Pseudo Genes (internal stop)      :: 21 of 103
            Pseudo Genes (multiple problems)  :: 14 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8328"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            907..1626
                     /locus_tag="EQB48_RS16530"
     CDS             907..1626
                     /locus_tag="EQB48_RS16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(1763..2179)
                     /locus_tag="EQB48_RS16535"
     CDS             complement(1763..2179)
                     /locus_tag="EQB48_RS16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2182..2466)
                     /locus_tag="EQB48_RS16540"
     CDS             complement(2182..2466)
                     /locus_tag="EQB48_RS16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            2832..3056
                     /locus_tag="EQB48_RS16545"
     CDS             2832..3056
                     /locus_tag="EQB48_RS16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     Typing          908..1626
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaggatatt tttgaaataa atatttattt ctgttataat ttgattgcaa ataagtgaac
       61 acgaaacaca cgaaagcccc agagaggtcg gattctctgg ggcttttttt gtgggctgag
      121 tcgccttaat taagttttac ttcttgtttc ggttattcaa ccagtaactg aacaaagcgc
      181 ttaaaagccc aacaagaagg gagcaaataa agttagtgaa cacgaaacac acctccttcc
      241 agtcgcaaga ctgtcggata ggcgacaatc aattatacaa taaaaaagca aaaataagcc
      301 taagtttttt attgccttgg ggttgttagg gcttgccctg accgcctgta aggcgcttta
      361 gaacgaagga catagttttt atcgaatctt gtcttagaac ggcttagacg agctctcagg
      421 acgccacaaa ggagtcccta atgaattatt tttggcgaag gagcctaaag gcgactgctg
      481 caagtaaaat gtgagagcgt agcgatcact ccttcttaca gcgaccatag ggagctagat
      541 tggcagttta atatcatgcg cgaacctgtt tattttaaaa agtgggtgat tttttggcgg
      601 gaaattttgc tcgcgcgcgc gcgtttattt tatttatttt ttaaagattt tttagaaccc
      661 tttttagggg gcagttgagc cttactctcc caacggatac agaagggtac taacaacttt
      721 ttatctgagt actaacaact ttttatctga gtactaacaa ctttttatct gagtactaac
      781 aactttttat ctgagtacta acaacttttt atctgagtac taatatcctt ttgtataata
      841 acaaccaacc tatttacgag ttggttgtta aaggttatta tttatttgta aggggggaag
      901 aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc aaaatgaatt gaatttagta
      961 ccgctgaaaa atttcaatgc taagcagatg gatttatttt ttgcattatg cgctcgtatg
     1021 aaagatcgtg gtttgaaaaa agttagattt tctttcgagg aattaaaaga gttaagtgat
     1081 tataagatga ctgctacaaa agcctttgtc ggtgatttag aaaagctcta taaagatatg
     1141 cttaatctta cttaccgtac tgaagatgag gattctattc gctactttgt tttgtttact
     1201 ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca gtgttaatcc ggacctagaa
     1261 tatattatta atggtttaac agctgaattt agtagatttg aattatctgc tttcacgtcg
     1321 ttgcgttcta cgtatgcaaa aactctattc cgactgctta tgcagtaccg ttctactggt
     1381 tattatgtag ttcgtattga agagtttagg agcttaatgg atatcccaga atactatcaa
     1441 atgggtaaca ttgatcaaaa agtattaaat cctgcattac gagaactaaa aaaatacttc
     1501 aatgatctta aagtcacaaa aataaaagca aaaaagggca ataaaatcgc caaattagaa
     1561 tttacttttt caagcctaac gtcagatttg ccaaagattt ctctgcgtaa ctggttagaa
     1621 gaataaataa aaaagaactc gcccccctta cattaagcaa gtagacaagt cccttacaac
     1681 cgactaagat tgctagtaca ttctagcata ttgtatgggg cttctaaata aaaaacagat
     1741 aggaataact cctatctgtt ttttatcgtt ttagaaaatt tgttgggtcg aaatttctag
     1801 aagtgactgt ttgactggtt tctttttgat gattctttgg tcgatttcga tttgatggat
     1861 ttagtttagc ttcgattttt tctttcactt cttgatatac tttagaattt gtgccaataa
     1921 gaacgcctgt atcttttaga tataaatcag caaattctgc gatgatttca gtcatgatgg
     1981 aaatttcttt tcccatagca tttagttttg cctgattgct ggtagtggtc gtgttggtat
     2041 cgggttcata actcatacat aaaaagcgaa tggcttccga gtagttattg atatctggat
     2101 ttttttcgat gatggaatct aaaacgtcaa catgactagg gtacaaagta acatgttttt
     2161 ggacagaagt ttttctcatg gttatatcat tcctccgata agcatttcaa tgatttgatt
     2221 atttgtgtag acatactcgc ttaatatttg agtattttct gtttggatag caataaattt
     2281 atcgagcaat aaatcgaatt ggtctttttt gtaagtcgta agttgacggt caaaatcact
     2341 ttcgattaaa acttttagat atttatttcg actccatttt tctcctgttt tattcgtgag
     2401 ttcttggcaa cgtttgtcta ttgttgcaac aaactttgga tcaatattcc gaactaaaat
     2461 gtccataaaa aatctccttt tgcaattatg tataatttat gtataccata caaatactat
     2521 atttgtatag ctgttttttg ccaaaatgct atacataagc tacccacgat ggcgaaaaag
     2581 tgatatcaaa tttttttctg agaaacgtga taggcacagc ctatctaccc acttttttta
     2641 gaaagtgggt acacgtttcc ttatgctctt taaaattcgg gggggagtgc tgcacgtgta
     2701 atcgataacc aaatttaaaa atgcacgatc aaaaatttat ttagtttttc aaacgacatt
     2761 ttttttgaga gtatagacgg caaagaagtg attttatttc tagaaaaatt gtgagactgg
     2821 agaaagaaaa aatggaaaaa tttccttacg aatattttca aaacttagaa aaattagagc
     2881 aagcaattca agcaatcaag ttcgatttaa cagacgaaaa attgttggaa tataaaaata
     2941 tgacacccga aagaaaacga tcgttggaaa aattaatgca actagtgaac gaagacggcg
     3001 gaatttcaga cgcagctttt gacgcatgga gcgaatttca tggagaatat aattaagtgg
     3061 gtttaaatgg ctctaaaatc gcttctaaga gttttttagc ttggaccaat gaaatacacc
     3121 aaaaactaaa taaaacgtct taaatcgaaa aatacgagct ctcagagcga ctttatttgt
     3181 aaatgttgca tataagaaat aattacggat tttagacttt t
//
