LOCUS       NZ_LR135413             2947 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8284 plasmid 6.
ACCESSION   NZ_LR135413
VERSION     NZ_LR135413.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885688
            Assembly: GCF_900639625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,245
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 31 of 110
            Pseudo Genes (incomplete)         :: 76 of 110
            Pseudo Genes (internal stop)      :: 21 of 110
            Pseudo Genes (multiple problems)  :: 14 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135413.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8284"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            <1..925
                     /locus_tag="EQB56_RS16905"
     CDS             <1..925
                     /locus_tag="EQB56_RS16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_164853020.1"
                     /translation="SNRRLNAQTMCTPLKMQWSIDRITIVGTLRENIYYHTPHDVLILD
                     FEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQEGKGRIDFNPNKISQFLA
                     GSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPVSFKPIYGRSGKLETAYW
                     GSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRGKATDWHAMVHESLDSFA
                     SPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELLKQESQNDELTNHLRETF
                     SESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            922..1062
                     /locus_tag="EQB56_RS16910"
     CDS             922..1062
                     /locus_tag="EQB56_RS16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(1399..1815)
                     /locus_tag="EQB56_RS16915"
     CDS             complement(1399..1815)
                     /locus_tag="EQB56_RS16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1817..2098)
                     /locus_tag="EQB56_RS16920"
     CDS             complement(1817..2098)
                     /locus_tag="EQB56_RS16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     Typing          3..922
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.511"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atctaacagg cggttaaatg cacaaactat gtgcaccccc ttaaaaatgc aatggtcaat
       61 tgaccgaatt accattgttg gtacattaag ggaaaatatt tattatcaca ctccccatga
      121 tgtgttgatt ttagattttg aacagcttat gcgtctaaac gaaggtaacg gatatttaaa
      181 agcggttgga aataatggct ggcaattact agaccaacat gaggaaaata tagcttatat
      241 tgagattctt aagtttcaag agggaaaagg gcggattgac tttaacccca ataaaattag
      301 ccaatttctg gcgggctcga tgaagaactt tatccatgat ttatttttag aaccacattt
      361 ctcacgtgca gatattgctt gtgacattat tgacgtgcca gatgatttca ttacccaata
      421 tcgggtggtt gatcctgttt ctttcaaacc aatttacggt cggtctggga aactggaaac
      481 agcatattgg ggaagtcgtg ctagtgaacg acagattcgg atgtataaca aaaaattgga
      541 acaagaaaga aaacggaaag ttgttccgaa agaaatcgat acttggtggc gactagaatt
      601 gcaattgcga agggggaaag caactgattg gcatgcaatg gttcatgaaa gtttagatag
      661 ctttgcaagt cctcactatt tgccagaaga tgtgaaagta actgacagaa tgatgattac
      721 tgctcttata acggaacata gttactgggg gcaaattcat agaaattcaa aatataaata
      781 tcgtgaattg ttaaagcaag aaagtcaaaa tgatgagttg acgaatcatt tgcgagaaac
      841 attttcggag tcggcagatg atttgaaacg agaattagat acatggcttc aaggacttga
      901 tgtgacggaa gtaggtgctg aatgagtgat tcgatagaaa aaatgaccct aaaaaaactt
      961 gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa ttcgggattt
     1021 gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt tatgtctttg
     1081 ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg ctctatgtga
     1141 aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta tttagctgct
     1201 cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa tttttttgtt
     1261 tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat tggttactat
     1321 cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac caggaattta
     1381 aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat ttttagaagt
     1441 aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg agggattaag
     1501 ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc caataagaat
     1561 acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca tgatggaaat
     1621 ttcttttccc atagcattta gtttggcttg attgctggta gtagtcgtgt tggtatcagg
     1681 ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat ctggatttct
     1741 ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt gtttttgaac
     1801 agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg ttgtttgtac
     1861 gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat ttttcgagta
     1921 ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg ttttcgatta
     1981 ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg agttcttggc
     2041 aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa atgtccatag
     2101 aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata gtatttgtat
     2161 agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa agtgatatca
     2221 aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt tagaaagtgg
     2281 gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc tgttcgtaaa
     2341 aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc atgtaatcaa
     2401 gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg ttcatttcgt
     2461 ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc ctgtaaggcg
     2521 actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa atgaagagcg
     2581 gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg aaattccctg
     2641 aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa aaaagagcaa
     2701 aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact actcacctag
     2761 ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta gagatttttg
     2821 gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc tcttttttct
     2881 ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg gaaaaactta
     2941 accgcat
//
