LOCUS       NZ_LR135412             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8284 plasmid 5.
ACCESSION   NZ_LR135412
VERSION     NZ_LR135412.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885688
            Assembly: GCF_900639625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,245
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 31 of 110
            Pseudo Genes (incomplete)         :: 76 of 110
            Pseudo Genes (internal stop)      :: 21 of 110
            Pseudo Genes (multiple problems)  :: 14 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135412.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8284"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(197..613)
                     /locus_tag="EQB56_RS16880"
     CDS             complement(197..613)
                     /locus_tag="EQB56_RS16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(616..900)
                     /locus_tag="EQB56_RS16885"
     CDS             complement(616..900)
                     /locus_tag="EQB56_RS16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1266..1490
                     /locus_tag="EQB56_RS16890"
     CDS             1266..1490
                     /locus_tag="EQB56_RS16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            2562..>3221
                     /locus_tag="EQB56_RS16900"
     CDS             2562..>3221
                     /locus_tag="EQB56_RS16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_164853019.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFT"
                     /name="replication initiation protein"
     Typing          2563..3221
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcaagcc taacgtcaga tttgccaaag atttctctgc gtaactggtt agaagaataa
       61 ataaaaaaga actcgccccc cttacattaa gcaagtagac aagtccctta caaccgacta
      121 agattgctag tacattctag catattgtat ggggcttcta aataaaaaac agataggaat
      181 aactcctatc tgttttttat cgttttagaa aatttgttgg gtcgaaattt ctagaagtga
      241 ctgtttgact ggtttctttt tgatgattct ttggtcgatt tcgatttgat ggatttagtt
      301 tagcttcgat tttttctttc acttcttgat atactttaga atttgtgcca ataagaacgc
      361 ctgtatcttt tagatataaa tcagcaaatt ctgcgatgat ttcagtcatg atggaaattt
      421 cttttcccat agcatttagt tttgcctgat tgctggtagt ggtcgtgttg gtatcgggtt
      481 cataactcat acataaaaag cgaatggctt ccgagtagtt attgatatct ggattttttt
      541 cgatgatgga atctaaaacg tcaacatgac tagggtacaa agtaacatgt ttttggacag
      601 aagtttttct catggttata tcattcctcc gataagcatt tcaatgattt gattatttgt
      661 gtagacatac tcgcttaata tttgagtatt ttctgtttgg atagcaataa atttatcgag
      721 caataaatcg aattggtctt ttttgtaagt cgtaagttga cggtcaaaat cactttcgat
      781 taaaactttt agatatttat ttcgactcca tttttctcct gttttattcg tgagttcttg
      841 gcaacgtttg tctattgttg caacaaactt tggatcaata ttccgaacta aaatgtccat
      901 aaaaaatctc cttttgcaat tatgtataat ttatgtatac catacaaata ctatatttgt
      961 atagctgttt tttgccaaaa tgctatacat aagctaccca cgatggcgaa aaagtgatat
     1021 caaatttttt tctgagaaac gtgataggca cagcctatct acccactttt tttagaaagt
     1081 gggtacacgt ttccttatgc tctttaaaat tcggggggga gtgctgcacg tgtaatcgat
     1141 aaccaaattt aaaaatgcac gatcaaaaat ttatttagtt tttcaaacga catttttttt
     1201 gagagtatag acggcaaaga agtgatttta tttctagaaa aattgtgaga ctggagaaag
     1261 aaaaaatgga aaaatttcct tacgaatatt ttcaaaactt agaaaaatta gagcaagcaa
     1321 ttcaagcaat caagttcgat ttaacagacg aaaaattgtt ggaatataaa aatatgacac
     1381 ccgaaagaaa acgatcgttg gaaaaattaa tgcaactagt gaacgaagac ggcggaattt
     1441 cagacgcagc ttttgacgca tggagcgaat ttcatggaga atataattaa gtgggtttaa
     1501 atggctctaa aatcgcttct aagagttttt tagcttggac caatgaaata caccaaaaac
     1561 taaataaaac gtcttaaatc gaaaaatacg agctctcaga gcgactttat ttgtaaatgt
     1621 tgcatataag aaataattac ggattttaga cttttgtagg atatttttga aataaatatt
     1681 tatttctgtt ataatttgat tgcaaataag tgaacacgaa acacacgaaa gccccagaga
     1741 ggtcggattc tctggggctt tttttgtggg ctgagtcgcc ttaattaagt tttacttctt
     1801 gtttcggtta ttcaaccagt aactgaacaa agcgcttaaa agcccaacaa gaagggagca
     1861 aataaagtta gtgaacacga aacacacctc cttccagtcg caagactgtc ggataggcga
     1921 caatcaatta tacaataaaa aagcaaaaat aagcctaagt tttttattgc cttggggttg
     1981 ttagggcttg ccctgaccgc ctgtaaggcg ctttagaacg aaggacatag tttttatcga
     2041 atcttgtctt agaacggctt agacgagctc tcaggacgcc acaaaggagt ccctaatgaa
     2101 ttatttttgg cgaaggagcc taaaggcgac tgctgcaagt aaaatgtgag agcgtagcga
     2161 tcactccttc ttacagcgac catagggagc tagattggca gtttaatatc atgcgcgaac
     2221 ctgtttattt taaaaagtgg gtgatttttt ggcgggaaat tttgctcgcg cgcgcgcgtt
     2281 tattttattt attttttaaa gattttttag aacccttttt agggggcagt tgagccttac
     2341 tctcccaacg gatacagaag ggtactaaca actttttatc tgagtactaa caacttttta
     2401 tctgagtact aacaactttt tatctgagta ctaacaactt tttatctgag tactaacaac
     2461 tttttatctg agtactaata tccttttgta taataacaac caacctattt acgagttggt
     2521 tgttaaaggt tattatttat ttgtaagggg ggaagaaagg aatggatgaa aaaacggaat
     2581 taacagtcaa atatcaaaat gaattgaatt tagtaccgct gaaaaatttc aatgctaagc
     2641 agatggattt attttttgca ttatgcgctc gtatgaaaga tcgtggtttg aaaaaagtta
     2701 gattttcttt cgaggaatta aaagagttaa gtgattataa gatgactgct acaaaagcct
     2761 ttgtcggtga tttagaaaag ctctataaag atatgcttaa tcttacttac cgtactgaag
     2821 atgaggattc tattcgctac tttgttttgt ttactggttt tgaaatcaat aaaacaaaaa
     2881 agtttgttga agtcagtgtt aatccggacc tagaatatat tattaatggt ttaacagctg
     2941 aatttagtag atttgaatta tctgctttca cgtcgttgcg ttctacgtat gcaaaaactc
     3001 tattccgact gcttatgcag taccgttcta ctggttatta tgtagttcgt attgaagagt
     3061 ttaggagctt aatggatatc ccagaatact atcaaatggg taacattgat caaaaagtat
     3121 taaatcctgc attacgagaa ctaaaaaaat acttcaatga tcttaaagtc acaaaaataa
     3181 aagcaaaaaa gggcaataaa atcgccaaat tagaatttac t
//
