LOCUS       NZ_LR135411             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8284 plasmid 4.
ACCESSION   NZ_LR135411
VERSION     NZ_LR135411.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885688
            Assembly: GCF_900639625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,245
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 31 of 110
            Pseudo Genes (incomplete)         :: 76 of 110
            Pseudo Genes (internal stop)      :: 21 of 110
            Pseudo Genes (multiple problems)  :: 14 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135411.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8284"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            complement(<1..1082)
                     /locus_tag="EQB56_RS16820"
                     /pseudo=""
     CDS             complement(<1..1082)
                     /locus_tag="EQB56_RS16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1136..1510)
                     /locus_tag="EQB56_RS16825"
     CDS             complement(1136..1510)
                     /locus_tag="EQB56_RS16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            1643..2104
                     /locus_tag="EQB56_RS16830"
     CDS             1643..2104
                     /locus_tag="EQB56_RS16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2416..2586)
                     /locus_tag="EQB56_RS17120"
     CDS             complement(2416..2586)
                     /locus_tag="EQB56_RS17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2602..3141)
                     /locus_tag="EQB56_RS16835"
     CDS             complement(2602..3141)
                     /locus_tag="EQB56_RS16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(3169..3906)
                     /locus_tag="EQB56_RS16840"
     CDS             complement(3169..3906)
                     /locus_tag="EQB56_RS16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            4551..4637
                     /locus_tag="EQB56_RS16845"
     CDS             4551..4637
                     /locus_tag="EQB56_RS16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4758..5447)
                     /locus_tag="EQB56_RS16850"
     CDS             complement(4758..5447)
                     /locus_tag="EQB56_RS16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5449..5727)
                     /locus_tag="EQB56_RS17485"
     CDS             complement(5449..5727)
                     /locus_tag="EQB56_RS17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(5687..6601)
                     /locus_tag="EQB56_RS16860"
     CDS             complement(5687..6601)
                     /locus_tag="EQB56_RS16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6583..6963)
                     /gene="mobC"
                     /locus_tag="EQB56_RS16865"
     CDS             complement(6583..6963)
                     /gene="mobC"
                     /locus_tag="EQB56_RS16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(7405..7542)
                     /locus_tag="EQB56_RS17125"
     CDS             complement(7405..7542)
                     /locus_tag="EQB56_RS17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(7656..8276)
                     /locus_tag="EQB56_RS16870"
     CDS             complement(7656..8276)
                     /locus_tag="EQB56_RS16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(8273..>9317)
                     /locus_tag="EQB56_RS16875"
                     /pseudo=""
     CDS             complement(8273..>9317)
                     /locus_tag="EQB56_RS16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     BGC             1..6510
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3170..3906)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5691..6601)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttattttctt ctacggtgtt gtttctattt aattgattag taatatttgt tttgtataaa
       61 ttagtagttc tttcccaata tttaagagaa ttattttcgg cttcattatg ggtatttatt
      121 acttttgtag aagctattaa cagaaggatc atgcttaaga atagacagat atttaatata
      181 caaagattta acttaagtgg tcgtgaattt tttagataat tttttatact agataaatga
      241 tagatactta gagtaactat gataaataaa aaactaatta taaaatgaaa aatactacat
      301 agaagaaatg ataggaaaaa attttcgtta ctgtaatata tgctcaaaaa aatagataaa
      361 atgaaagtta taatcaaaga acaaaataag aggtttaact ctttaataga atcttttata
      421 gtatcaaaag agttatagcc taacattttt tttagagtga tttctttcat ttttgaaaaa
      481 gcaaatataa ttgtagtaat tacaataaat atactaagat agcagataac aagaacaata
      541 ttagtattaa tttgaaaagt atctgattga ttgatattgg aaatagtagg tgttccatat
      601 tttttcagta caggaattaa tttgtttgag tttccttgta catataatat ttcacttaat
      661 ccaatatttt ccacatgaga aaaaggataa atatttatat ttaaaaaatt atttggaagt
      721 tttaattggt acttagaatt tttgttatta gtataatgga ctttagaatc gtttcttgct
      781 aatatatttt ttttagaaaa tttccagtgt ttgttagcgt tagggtttgt agcaagaata
      841 gttagttggt taacagcagt aaaactatat tgagaaatat taatattttc ttgtgtagat
      901 atttcataca aagcgtttat ataactgtta aagtccatat catctttata ttgcatatga
      961 atttcagcgg tatttttata tagcgcttca gaagtcttta taccatttat aacattagta
     1021 gaaaaaaata gtattgtaat gcagacaaaa agtgataaaa cagcgtttat tttttttaac
     1081 atattttgaa tttcctttca aatgtaaata aaaaagagag tttaaactct cttttttaat
     1141 aaacattcca ccaaccagta tttccagtaa gtgtggcatt gcatgttgca taagctctta
     1201 ctccagcttt ctgaactgat cgtacttgac cgcctcgtcc atttttagct gttgcactat
     1261 gtttagctct agcatggtag taatttgcat aaactttaaa tccattaacc ccacagttga
     1321 aatccccgcc gccagcagca tatctcaatt tactagaaga aaatccttct tctgtagctt
     1381 ccattcctcc tccgtaaatg gcggtatggt cttcgggtac atctagattt aaagcttctg
     1441 tggcactagc tattcctcca ctagttaata agacacccat taaagcagaa agacataaaa
     1501 attttttcat aacttatttc tcctatcttt tttactagaa ttattttatt ttagtgtgtt
     1561 ttataaaata aagaactttt ttggcgcgtt agtattttta tgataaaatt ttatgtaaat
     1621 aatcgtttac ataggagaaa aaatggagga tttaggatct aagctaagag aattaagagt
     1681 gaaaaaaggg tatactcaag aatatgtggc aagaaaattt aacgttacac gtcaaactat
     1741 ttcaaagtgg gaaaataaca aaacaacacc agatatagag acactaaaaa aaatttgtga
     1801 attttatagt actaacataa attctatttt aaatgaagat aattcaaaaa ataaaaaaaa
     1861 taatttactg gtattgacag ctattatttt taatatattt gttactatat taggtttatt
     1921 tgaattgatt caaatgaatt tttttagctt tacacatgga atatttactt ggtttgctct
     1981 atctggatta atagtaatag atatcttaaa ttatttttat atttactatt cttatttaaa
     2041 aaatgaaaaa tttttttata caatgttttt ccccctagtt attttgatta ttttattaag
     2101 ctaatatgaa gtagttagtt atttaattgc atagttgaca taatgtcgac tatgccaagt
     2161 agcccctcta tcaagggcta tagctgctca ctttgtgatt tttaattggt ctagctaaaa
     2221 gcaactttct aaagcttgat ttttcgcggt tatctatatc taattctttg tgcattttta
     2281 tctgtcattt ttttaagact tgttattttt tgtaaaatat ggtaaatttt tggcagcgct
     2341 tcataagcgc gtgctttttc aagaataaac actcattatt ttttaactgg ctgctcggaa
     2401 gagcagtctt ttttattatt tatccgtctc ttttacaaac aattcttcgt taggactgtt
     2461 gtaataattt ggattcaatt tcaattcacc atgtttattc cctttattga ttactgacaa
     2521 taacatcgga ctgaagccta gaattcctag taaaatagaa ccgaatagaa gattttttct
     2581 tgtcatgaat gttccctcca gttatttcca aagttgccac catttttgct tcttcttttt
     2641 tgtgtgtttt tctatcgatt ctgtttcttc agaaagaata gtagttgatt catcttctgg
     2701 agtttcttcc gtataagcta attgtaactg ttcttgcagt ttttcgatct gttgatttgc
     2761 ctgtagggtg agctgttgtt gctggtcgag taatttttgc atattagaat tattttgctc
     2821 tttgagggaa agttgttctc gtaaaaattc attttcttca cgcaacttat ctagcaactc
     2881 gtttacaatc acgtttttgc ttgatatata aacgttttta tcgttttctt gttcatactc
     2941 atgcaaccta tatagtaacc tttggttacc taagttgcta gcaagttgca atactccaga
     3001 acgtaataca accttataac cacgctcatt tttagctaat aaggacgggt ctaatttatc
     3061 aacatgttta gagattgctt gcctacttat ttcaaataat tcagataatt cctttattgt
     3121 aaaggtttct ttgggttgca tgataattgc ctcctaaatt gttaggtatt atttctctaa
     3181 ccaattatct aaggtaattg aaggtatatt ttcttcaaaa taaatttgca gagatcctat
     3241 cttatttcct ttgtaggctt taattttttt gatttcaaaa gttttaaaaa tagcagggtg
     3301 agttgaatta acgtttaaaa attctttttt cgcttgggca aaaatttgtt tatcaatatc
     3361 tctcatgcga taactttctg gtattgctaa tagagctcta aaatcttcta tttttacaaa
     3421 ccagtgacct tttttagatc ttctgtcttt atgagccata agttggcgat ataactcttt
     3481 aacgtagcta ctctttaagt ttgtcatatt ttctaattcg aaacgagtaa aattagttcc
     3541 gattgaatta agtatatatt caaattcttc agaaaccttt attaataatg tttcattcac
     3601 tttatcgatt ttaaattttt gaaacagatt taattgttca tatgtttttt cgtcttcata
     3661 aataaaagac aaagtaatga ttttttttga catttttatt aaatcattag caaaacggtc
     3721 gccacgtgca tttatattgt aatcaattaa ttcttttatt tgtttaaatg taaaaatttc
     3781 ttcttttgta ccttttcttt tcattttaga acatattccc caaaataaat ccatatctat
     3841 aggggtaaat tttcttaaag ggacagaatt taatccatta ttatatcgaa ctatatcgtt
     3901 actcaataaa aatcacctca ataaattatg ttatcataga cataagagga tgtaaaacac
     3961 aatttatccc ccatagtgtg acaatttatc ccccatagtg tgacaattta tcccccatag
     4021 tgtgacaatt tatcccccat agtgtgacaa tttatccccc actacctatg tcaatactta
     4081 ctctcccata aggttgctaa tccctaacag ttattattaa cagtatatct aacagtatat
     4141 ctaacagtat atcttaagag cacattgaat ttcccgccta aaaaactcct actttttcct
     4201 ccaaaaaggt tcgcgaattt ttctaaactg gaaaacttgc tccctacggt cgctaaaaag
     4261 gtgtgatcgc tatgctctca tattatactg cgcagtcgcc tccggctcct ttgctaaaac
     4321 cgttgttcaa agaggaggtg tgagactctg aaagcttgtc taagccgttc taagacaatt
     4381 cttgattaat ttcccttaat ctagtcgaga agcgccttac aggcggtcag ggcaagccct
     4441 aacaacccta aaacaagtga tacttaaaaa caccacacaa atttaatttt ttaagatata
     4501 ataacttgtc gcctatccga cagtcttgcg actggaagga ggtgtatcta atgtttgaac
     4561 tgttcatttg cccccttctt gtaggagttt gtgtctcttt attcagttat tggttgaata
     4621 accgaaacaa gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag ccccagagaa
     4681 tccgacctct ctggggcttt cgtgtatcta atgtttgatc atttgcacat tcagtttaac
     4741 atgattttaa atcaaattca attttgttgt tgctgatcta accattcctt gacacttttt
     4801 agatagttta aggcggtttc tttttttgtc ggattttgca tgttagttgc ttgttgaagt
     4861 aagatttcat agcgttcata tgtgacagat tgttgttttt cttttttttc ttcttcactg
     4921 gcaaaccaag ggtttgtgtc tccaaataga tcactacagg cttcttctat ctttgtatct
     4981 tttacatatt gcacgtcttt tttggttcgc tcatagttgt cttgaacctc tttttgctgt
     5041 ttttctggca agttttgata aagacgagtg agtttttcca tgtttttatc cgtcaaacgt
     5101 ttttcttgat tcacaaaaat atcgttcatt tcctcataac aagtggtaaa ctcttccatt
     5161 ttttgtgcta atgcttgctc ttttttcttt ttatcacgcg ctgattcata cttttgagtg
     5221 acatttccta ataaaatact tctagaaacg cgcttatttc cctctaaaac gccgaataag
     5281 ccgcttgaaa cgattatcaa ggaaaatata cagccaagaa agaaaaatcg cttaaatcga
     5341 attttagagc cttttaatga atatcttgtt tgaaaaattt tatagatgag ccaaatactt
     5401 cctgaaatac taacaaaaat gagtagatag ggtaaataaa atgccatttt agcctcccca
     5461 tactaaccaa aatgcgaaca gtgtcaggac taagcaacat aagggggcaa gaaaatccaa
     5521 tgatcgttga ataagcacct ttttctgttc cttatttcgc tggtctaata gctcttgatg
     5581 gagtttttta acttgtaaat caatcgtagt ttgtatcctt tcttgcgtat aaggaacgag
     5641 aaaagcacgt tgtttcttta tttcgttttc tatgtctttt aacgttttag agcgataatt
     5701 cttgttggtg gtctgttcct ctttcaacgt gttgttcatg gtttcttcga gccggtctaa
     5761 tttccggttg ttcaggcgta agagttcgtt gttctcttcg tacttcttct gcagtttcgc
     5821 gaatgctagc tcgagctctt cgtagctcgt tggttcgctg ttggttatag gttcgttctc
     5881 gtctgtccag ctcatgtaaa atcgtctcct tttcgtagtc ctttcctagt ctgttggatc
     5941 gtactttttt ctttttgtgg atcaattcgt aagtgaaatt tttcccgcga ttgaagcaat
     6001 gaatccccaa tgttttgagg gaattacaaa attggtcata ggttctggtt tttttgtttt
     6061 tcattgtttg atcgatcgca atccgaattt cgtctttcca aacaggttca tttttttctt
     6121 ttaattttat ttcagacatg gttcgttttt ctggtttctt tcctcgctca gtgattggta
     6181 agttatgtgc aagacaaatc tcgtcattta ggtcataaca tttgtttaga aatgcttgac
     6241 catgagcttg aaattttttt ccagtctcca gattaacgga gttaatgaca atgtgattat
     6301 gaatatggtc tttgtctgta tgtgtgtaaa tagctgtttg gtgatcgggg gctatttttt
     6361 ttgcatactc tgcccctagt tgattagctt tttcagcggt aatttctcct ggtcgaaaag
     6421 attgaatcaa taagtgtgcc tgtacacggt cgtcttttcc atacagcatg cgaatttgtt
     6481 tcatttcact tttggcataa tcgacgtcac agtttattcc tgttttaatg gttgctcgtg
     6541 gttctgcgta attaattgca cgactacaag aagtggttcg gcttacctta attgttgcca
     6601 tatcttttgt agctcctttt gcatgatttt taggttttcg gataaacgtt gagcgtcacc
     6661 agaagcaacg tcatgttggt gtgtattgca ccacttagca atttgattga tattccctcc
     6721 agctttggct aaatcgcgcg cgattgcctg ggcaatgtct ggcgccactt tcggtgtcac
     6781 cagacgcgcg ttttgtgcct tggctttcac gaaagccggc acactcattt gcaaagcttc
     6841 agccgattgc ttcagctttg caaattcgga ttcgctcact cgaaaactga tttgttttgg
     6901 ctcggatcga taggtatatt ttttagaaga attgctagcc aaattttgat tttgttcgct
     6961 cataagtccc accctttcag tacgtcctac ggacgtatcg ttcgcaatac cattgtacct
     7021 aaaaaggtac aatgagtaaa gctcactaaa agggtttggc aagctctata tttgctagcc
     7081 aaatacctac gtatttagtg caaataaccg cttgtcaaag gggcgcaagc tcccccttga
     7141 atccccctca aaagccacgc tccacaatac aaaaaaagct agcaaatgct agccactttt
     7201 tgtaaagttt cgcttatttc accgaagatg aaattgttac tttgcaacaa aaaacataaa
     7261 tgcttttttg tgtaaactac taactattta gctaaaactg atagttttat taaacattca
     7321 gcaatatttt tagccaattt tttatcagac tttcggcgag tcattatgat aatatgtcca
     7381 ctttttgtta cttctaccaa aaatctagca ataactctcc cattcttcag ttgatttagt
     7441 ttcataagtc ttttgtaatt gacggtttaa tttttttggc aattgcaaag aataaataag
     7501 tctatcataa aatttttcat actctttttt caataaaatc acttgggtgc ctccttttta
     7561 ttaagtgatt atttaaattt tgggatagga gagatggaat ataaaataaa tacccgtggt
     7621 gctagtttta aatcagctag catcacgggt aaaaattata attctataat attagttcca
     7681 tagtttagaa catttaagtc atgtgttact agtacaacgg cttttttttc tttttctgtt
     7741 aaatttttca gaaagttcat tataaaagtt gagttttctt tatctaaatt tcctgtaggt
     7801 tcatctgcaa ataatatatc aaatggtttt aacatatttc taattaatgc tattctttgt
     7861 tgttctccac cgcttaagag aaagattttt tcttttaaga tatcttttgg caaagaaaaa
     7921 tctgttaata aattataaat atgatcattg gaaatattac tgttaaactt ttttgctaat
     7981 tcgaaattgt attgtactgt ttgattttca agaagagcat agttttgaaa taaatagcta
     8041 atatgatttc ttcgtagacg ttgtatatca tgtttagaaa ctttcttttc aaaatagaat
     8101 aagttaccgg tatattgttt atctattagg cttaaattat ttaacagagt agtttttcca
     8161 ctaccacttt ttcctgttac tataataaaa tctttttctg atattgaaaa gttccaattt
     8221 tgtttgatct tttttttatt aattgacagg gtgaaattct caactgataa tatcataaaa
     8281 tattctcctt aaaatattta aaactatttt ttttataaat aataatagaa gtaattagat
     8341 ttattaaaaa gatcataaaa attataaaat acaagtaagt gttttttgaa aatatcaatt
     8401 gaaaacacat agtagcaaat aactctattg taaaagggag taaacataaa tctaaaactt
     8461 gacttattgg tgaatatcca aatatttttt ttaaagctat tttttttgaa tttatagtaa
     8521 aactaacaaa aatcatttga aaattaatta ttataaataa tcctaatatt aaaagatatt
     8581 gtagatatgc atttaattta tttacttttt tttgagataa taattcacca gtttcttgaa
     8641 aaacagaata agaaaaattt atgatatttt ctaggttaaa ttcttttata tacggattaa
     8701 tttgattaaa aaagttttgg tttgaagtat gtttctgatc aaaaaataat ccaccatcac
     8761 tagtcaatat atttccccaa aataatgatt ctgttatttc tggattaata actctaacta
     8821 tgggatcagt tattgtatag ttatcttttg cctttccata taaggtatta aaactaaaat
     8881 acttttggtt attattaaca aatattagtt ttatttttaa atttttcaaa ttaggatttt
     8941 tataaggagc ttgaggagga gagtcctcaa gtctaaactg tatgtcttct aaataatttt
     9001 tttcaatcat ttttgcatat tctttatatt ttttaggtac tagaatgtaa gtagttagtg
     9061 gatcagagtt tattaaattt aagtcagtag attcttcaaa agaaatagga ttgtaatatt
     9121 ttagatagtt taaatcaaca catattcctg caccagcagg ttgactaaca tagtcttcaa
     9181 aatttggata aactttttgt ccaataaaaa attctttatt attctcttgg atagttgcgt
     9241 aattgtatgg cgcgataata aaagttttat aattcttctg tattttataa acaaatttac
     9301 tagctttttt caagtag
//
