LOCUS       NZ_LR135407             2947 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8377 plasmid 7.
ACCESSION   NZ_LR135407
VERSION     NZ_LR135407.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885755
            Assembly: GCF_900639595.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,048
            CDSs (with protein)               :: 3,048
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 35 of 111
            Pseudo Genes (incomplete)         :: 76 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 17 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135407.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8377"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            815..1789
                     /locus_tag="EQB54_RS16975"
     CDS             815..1789
                     /locus_tag="EQB54_RS16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1786..1926
                     /locus_tag="EQB54_RS16980"
     CDS             1786..1926
                     /locus_tag="EQB54_RS16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(2263..2679)
                     /locus_tag="EQB54_RS16985"
     CDS             complement(2263..2679)
                     /locus_tag="EQB54_RS16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2681..>2947)
                     /locus_tag="EQB54_RS16990"
     CDS             complement(2681..>2947)
                     /locus_tag="EQB54_RS16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127822619.1"
                     /translation="RKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDFDHALM
                     HYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     Typing          840..1786
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aactaaaatg tccatagaaa aactcctttc gcatttgtgt ataatttatg tatatcatac
       61 gaatatagta tttgtatagc tgttttttgc taaaatacta tacataagct agccacgatg
      121 gcgaaaaagt gatatcaaat ttttttctga gaaacgtgat aggcacagcc tatctaccca
      181 ctttttttag aaagtgggta cacgtttcct tatgctcttt ataaaatggt gatgtgcgac
      241 ttttttctgt tcgtaaaaat aaaaaaagtt tacgaaaatg aaactaaact gaacgattat
      301 gacgggcatg taatcaagat gaaatgattt tataagcgcg tattttgcgt tctaagacgt
      361 tttagcgttc atttcgttta gttatcggca caatcgttaa aacacgtaag agaagcgaga
      421 atggagcctg taaggcgact ggtagcgaaa cggcgtgtta gattgtgcaa gccgagaact
      481 gaatgaaatg aagagcggaa gcgtgaaata atttcggcag gaggaggctc aaggggttcg
      541 gggaatgaaa ttccctgagg tctttttatt tttttaagag gagtgctata atcaaagaaa
      601 acgagaaaaa agagcaaaat aaaagccgaa cggtgttgga agcacccttc ggctaattac
      661 ggaaactact cacctagttc cgtaactcaa attcattata cgaatatttt taggtttctt
      721 caagctagag atttttggat atggattttt agagttacgt gaactctgga aattctttcc
      781 aaaaatctct tttttctttg aggaggaaca aaatttgagt aaacgtggca cgatcataaa
      841 tgagtgggaa aaacttaacc gcatatctaa caggcggtta aatgcacaaa ctatgtgcac
      901 ccccttaaaa atgcaatggt caattgaccg aattaccatt gttggtacat taagggaaaa
      961 tatttattat cacactcccc atgatgtgtt gattttagat tttgaacagc ttatgcgtct
     1021 aaacgaaggt aacggatatt taaaagcggt tggaaataat ggctggcaat tactagacca
     1081 acatgaggaa aatatagctt atattgagat tcttaagttt caagagggaa aagggcggat
     1141 tgactttaac cccaataaaa ttagccaatt tctggcgggc tcgatgaaga actttatcca
     1201 tgatttattt ttagaaccac atttctcacg tgcagatatt gcttgtgaca ttattgacgt
     1261 gccagatgat ttcattaccc aatatcgggt ggttgatcct gtttctttca aaccaattta
     1321 cggtcggtct gggaaactgg aaacagcata ttggggaagt cgtgctagtg aacgacagat
     1381 tcggatgtat aacaaaaaat tggaacaaga aagaaaacgg aaagttgttc cgaaagaaat
     1441 cgatacttgg tggcgactag aattgcaatt gcgaaggggg aaagcaactg attggcatgc
     1501 aatggttcat gaaagtttag atagctttgc aagtcctcac tatttgccag aagatgtgaa
     1561 agtaactgac agaatgatga ttactgctct tataacggaa catagttact gggggcaaat
     1621 tcatagaaat tcaaaatata aatatcgtga attgttaaag caagaaagtc aaaatgatga
     1681 gttgacgaat catttgcgag aaacattttc ggagtcggca gatgatttga aacgagaatt
     1741 agatacatgg cttcaaggac ttgatgtgac ggaagtaggt gctgaatgag tgattcgata
     1801 gaaaaaatga ccctaaaaaa acttgctgat gaattgggtg tatcgaaaaa tacggtgcgc
     1861 aatcaactgg tcaattcggg atttgacctt gctcagtata aagaaaaaaa tgcagtggtc
     1921 aattgaccga aatttatgtc tttgggtttt ggggttttaa aacgaaaaaa agggggcgat
     1981 tttcgccccc tttgctctat gtgaaaaatt tgttatactt attttatcct taaaacatac
     2041 caatcttttt cttatttagc tgctcgtacg agcagctttt tttacgatta tgaattcaaa
     2101 tcatgtattt ataatttttt tgtttttatc tttttttctg ttatttcttc tgaaattcgt
     2161 ttttttcatt caattggtta ctatcttatt gtaaatgttg ctattaagat ggtgatccta
     2221 cccaaaaaat aaaccaggaa tttaaatctc ctggtttatt ttttatcttt ttagaaaatt
     2281 tgttgggtcg aaatttttag aagtaaatgt ttgattggtt tctttttgac gattttttgg
     2341 ttgatttcgt ttggagggat taagttgttt ttcaattttt tctttcgttt cttgatacac
     2401 tttagaattt gtgccaataa gaatacctgt atcttttaga tataaatcag caaattctgc
     2461 aatgatttcg gacatgatgg aaatttcttt tcccatagca tttagtttgg cttgattgct
     2521 ggtagtagtc gtgttggtat caggttcata actcatacat aaaaaacgaa tggcttccga
     2581 gtaattgttg atatctggat ttctttcgat aatggaatct aaaacgtcca cgtgactggg
     2641 ataaagggta acgtgttttt gaacagaagt ttttctcatg ttatcttcct ccgataagtg
     2701 tttcaatgag ttggttgttt gtacgaacat attcattcag cgtttcagta tttttttctt
     2761 gaatagcgat aaatttttcg agtaataaat cgaattgatc tttcttgtaa tgcataagtg
     2821 cgtggtcaaa atcgttttcg attaggactt ttaagtagcg atttcgactc cattttttgc
     2881 cagttttatt tgtgagttct tggcaacgtt tgtctatttt tgcaataacc tttgaatcga
     2941 tcttccg
//
