LOCUS       NZ_LR135406             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8377 plasmid 6.
ACCESSION   NZ_LR135406
VERSION     NZ_LR135406.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885755
            Assembly: GCF_900639595.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,048
            CDSs (with protein)               :: 3,048
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 35 of 111
            Pseudo Genes (incomplete)         :: 76 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 17 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135406.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8377"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            393..617
                     /locus_tag="EQB54_RS16950"
     CDS             393..617
                     /locus_tag="EQB54_RS16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1689..2408
                     /locus_tag="EQB54_RS16960"
     CDS             1689..2408
                     /locus_tag="EQB54_RS16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(2545..2961)
                     /locus_tag="EQB54_RS16965"
     CDS             complement(2545..2961)
                     /locus_tag="EQB54_RS16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2964..>3221)
                     /locus_tag="EQB54_RS16970"
     CDS             complement(2964..>3221)
                     /locus_tag="EQB54_RS16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127822618.1"
                     /translation="PKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDFDRQLTTYKK
                     DQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     Typing          1690..2408
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcaatattc cgaactaaaa tgtccataaa aaatctcctt ttgcaattat gtataattta
       61 tgtataccat acaaatacta tatttgtata gctgtttttt gccaaaatgc tatacataag
      121 ctacccacga tggcgaaaaa gtgatatcaa atttttttct gagaaacgtg ataggcacag
      181 cctatctacc cacttttttt agaaagtggg tacacgtttc cttatgctct ttaaaattcg
      241 ggggggagtg ctgcacgtgt aatcgataac caaatttaaa aatgcacgat caaaaattta
      301 tttagttttt caaacgacat tttttttgag agtatagacg gcaaagaagt gattttattt
      361 ctagaaaaat tgtgagactg gagaaagaaa aaatggaaaa atttccttac gaatattttc
      421 aaaacttaga aaaattagag caagcaattc aagcaatcaa gttcgattta acagacgaaa
      481 aattgttgga atataaaaat atgacacccg aaagaaaacg atcgttggaa aaattaatgc
      541 aactagtgaa cgaagacggc ggaatttcag acgcagcttt tgacgcatgg agcgaatttc
      601 atggagaata taattaagtg ggtttaaatg gctctaaaat cgcttctaag agttttttag
      661 cttggaccaa tgaaatacac caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc
      721 tctcagagcg actttatttg taaatgttgc atataagaaa taattacgga ttttagactt
      781 ttgtaggata tttttgaaat aaatatttat ttctgttata atttgattgc aaataagtga
      841 acacgaaaca cacgaaagcc ccagagaggt cggattctct ggggcttttt ttgtgggctg
      901 agtcgcctta attaagtttt acttcttgtt tcggttattc aaccagtaac tgaacaaagc
      961 gcttaaaagc ccaacaagaa gggagcaaat aaagttagtg aacacgaaac acacctcctt
     1021 ccagtcgcaa gactgtcgga taggcgacaa tcaattatac aataaaaaag caaaaataag
     1081 cctaagtttt ttattgcctt ggggttgtta gggcttgccc tgaccgcctg taaggcgctt
     1141 tagaacgaag gacatagttt ttatcgaatc ttgtcttaga acggcttaga cgagctctca
     1201 ggacgccaca aaggagtccc taatgaatta tttttggcga aggagcctaa aggcgactgc
     1261 tgcaagtaaa atgtgagagc gtagcgatca ctccttctta cagcgaccat agggagctag
     1321 attggcagtt taatatcatg cgcgaacctg tttattttaa aaagtgggtg attttttggc
     1381 gggaaatttt gctcgcgcgc gcgcgtttat tttatttatt ttttaaagat tttttagaac
     1441 cctttttagg gggcagttga gccttactct cccaacggat acagaagggt actaacaact
     1501 ttttatctga gtactaacaa ctttttatct gagtactaac aactttttat ctgagtacta
     1561 acaacttttt atctgagtac taacaacttt ttatctgagt actaatatcc ttttgtataa
     1621 taacaaccaa cctatttacg agttggttgt taaaggttat tatttatttg taagggggga
     1681 agaaaggaat ggatgaaaaa acggaattaa cagtcaaata tcaaaatgaa ttgaatttag
     1741 taccgctgaa aaatttcaat gctaagcaga tggatttatt ttttgcatta tgcgctcgta
     1801 tgaaagatcg tggtttgaaa aaagttagat tttctttcga ggaattaaaa gagttaagtg
     1861 attataagat gactgctaca aaagcctttg tcggtgattt agaaaagctc tataaagata
     1921 tgcttaatct tacttaccgt actgaagatg aggattctat tcgctacttt gttttgttta
     1981 ctggttttga aatcaataaa acaaaaaagt ttgttgaagt cagtgttaat ccggacctag
     2041 aatatattat taatggttta acagctgaat ttagtagatt tgaattatct gctttcacgt
     2101 cgttgcgttc tacgtatgca aaaactctat tccgactgct tatgcagtac cgttctactg
     2161 gttattatgt agttcgtatt gaagagttta ggagcttaat ggatatccca gaatactatc
     2221 aaatgggtaa cattgatcaa aaagtattaa atcctgcatt acgagaacta aaaaaatact
     2281 tcaatgatct taaagtcaca aaaataaaag caaaaaaggg caataaaatc gccaaattag
     2341 aatttacttt ttcaagccta acgtcagatt tgccaaagat ttctctgcgt aactggttag
     2401 aagaataaat aaaaaagaac tcgcccccct tacattaagc aagtagacaa gtcccttaca
     2461 accgactaag attgctagta cattctagca tattgtatgg ggcttctaaa taaaaaacag
     2521 ataggaataa ctcctatctg ttttttatcg ttttagaaaa tttgttgggt cgaaatttct
     2581 agaagtgact gtttgactgg tttctttttg atgattcttt ggtcgatttc gatttgatgg
     2641 atttagttta gcttcgattt tttctttcac ttcttgatat actttagaat ttgtgccaat
     2701 aagaacgcct gtatctttta gatataaatc agcaaattct gcgatgattt cagtcatgat
     2761 ggaaatttct tttcccatag catttagttt tgcctgattg ctggtagtgg tcgtgttggt
     2821 atcgggttca taactcatac ataaaaagcg aatggcttcc gagtagttat tgatatctgg
     2881 atttttttcg atgatggaat ctaaaacgtc aacatgacta gggtacaaag taacatgttt
     2941 ttggacagaa gtttttctca tggttatatc attcctccga taagcatttc aatgatttga
     3001 ttatttgtgt agacatactc gcttaatatt tgagtatttt ctgtttggat agcaataaat
     3061 ttatcgagca ataaatcgaa ttggtctttt ttgtaagtcg taagttgacg gtcaaaatca
     3121 ctttcgatta aaacttttag atatttattt cgactccatt tttctcctgt tttattcgtg
     3181 agttcttggc aacgtttgtc tattgttgca acaaactttg g
//
