LOCUS       NZ_LR135404             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8377 plasmid 4.
ACCESSION   NZ_LR135404
VERSION     NZ_LR135404.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885755
            Assembly: GCF_900639595.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,250
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,048
            CDSs (with protein)               :: 3,048
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 35 of 111
            Pseudo Genes (incomplete)         :: 76 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 17 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135404.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8377"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            250..630
                     /gene="mobC"
                     /locus_tag="EQB54_RS16845"
     CDS             250..630
                     /gene="mobC"
                     /locus_tag="EQB54_RS16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            612..1526
                     /locus_tag="EQB54_RS16850"
     CDS             612..1526
                     /locus_tag="EQB54_RS16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1486..1764
                     /locus_tag="EQB54_RS17550"
     CDS             1486..1764
                     /locus_tag="EQB54_RS17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            1766..2455
                     /locus_tag="EQB54_RS16860"
     CDS             1766..2455
                     /locus_tag="EQB54_RS16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(2576..2662)
                     /locus_tag="EQB54_RS16865"
     CDS             complement(2576..2662)
                     /locus_tag="EQB54_RS16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3307..4044
                     /locus_tag="EQB54_RS16870"
     CDS             3307..4044
                     /locus_tag="EQB54_RS16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            4072..4611
                     /locus_tag="EQB54_RS16875"
     CDS             4072..4611
                     /locus_tag="EQB54_RS16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            4627..4797
                     /locus_tag="EQB54_RS17180"
     CDS             4627..4797
                     /locus_tag="EQB54_RS17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5109..5570)
                     /locus_tag="EQB54_RS16880"
     CDS             complement(5109..5570)
                     /locus_tag="EQB54_RS16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            5703..6077
                     /locus_tag="EQB54_RS16885"
     CDS             5703..6077
                     /locus_tag="EQB54_RS16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            6131..8257
                     /locus_tag="EQB54_RS16890"
     CDS             6131..8257
                     /locus_tag="EQB54_RS16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            8254..8874
                     /locus_tag="EQB54_RS16895"
     CDS             8254..8874
                     /locus_tag="EQB54_RS16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            8988..9125
                     /locus_tag="EQB54_RS17185"
     CDS             8988..9125
                     /locus_tag="EQB54_RS17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     BGC             703..9317
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3308..4044
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          613..1523
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaaacttta caaaaagtgg ctagcatttg ctagcttttt ttgtattgtg gagcgtggct
       61 tttgaggggg attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcactaaat
      121 acgtaggtat ttggctagca aatatagagc ttgccaaacc cttttagtga gctttactca
      181 ttgtaccttt ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg tactgaaagg
      241 gtgggactta tgagcgaaca aaatcaaaat ttggctagca attcttctaa aaaatatacc
      301 tatcgatccg agccaaaaca aatcagtttt cgagtgagcg aatccgaatt tgcaaagctg
      361 aagcaatcgg ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc caaggcacaa
      421 aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg cccaggcaat cgcgcgcgat
      481 ttagccaaag ctggagggaa tatcaatcaa attgctaagt ggtgcaatac acaccaacat
      541 gacgttgctt ctggtgacgc tcaacgttta tccgaaaacc taaaaatcat gcaaaaggag
      601 ctacaaaaga tatggcaaca attaaggtaa gccgaaccac ttcttgtagt cgtgcaatta
      661 attacgcaga accacgagca accattaaaa caggaataaa ctgtgacgtc gattatgcca
      721 aaagtgaaat gaaacaaatt cgcatgctgt atggaaaaga cgaccgtgta caggcacact
      781 tattgattca atcttttcga ccaggagaaa ttaccgctga aaaagctaat caactagggg
      841 cagagtatgc aaaaaaaata gcccccgatc accaaacagc tatttacaca catacagaca
      901 aagaccatat tcataatcac attgtcatta actccgttaa tctggagact ggaaaaaaat
      961 ttcaagctca tggtcaagca tttctaaaca aatgttatga cctaaatgac gagatttgtc
     1021 ttgcacataa cttaccaatc actgagcgag gaaagaaacc agaaaaacga accatgtctg
     1081 aaataaaatt aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg attgcgatcg
     1141 atcaaacaat gaaaaacaaa aaaaccagaa cctatgacca attttgtaat tccctcaaaa
     1201 cattggggat tcattgcttc aatcgcggga aaaatttcac ttacgaattg atccacaaaa
     1261 agaaaaaagt acgatccaac agactaggaa aggactacga aaaggagacg attttacatg
     1321 agctggacag acgagaacga acctataacc aacagcgaac caacgagcta cgaagagctc
     1381 gagctagcat tcgcgaaact gcagaagaag tacgaagaga acaacgaact cttacgcctg
     1441 aacaaccgga aattagaccg gctcgaagaa accatgaaca acacgttgaa agaggaacag
     1501 accaccaaca agaattatcg ctctaaaacg ttaaaagaca tagaaaacga aataaagaaa
     1561 caacgtgctt ttctcgttcc ttatacgcaa gaaaggatac aaactacgat tgatttacaa
     1621 gttaaaaaac tccatcaaga gctattagac cagcgaaata aggaacagaa aaaggtgctt
     1681 attcaacgat cattggattt tcttgccccc ttatgttgct tagtcctgac actgttcgca
     1741 ttttggttag tatggggagg ctaaaatggc attttattta ccctatctac tcatttttgt
     1801 tagtatttca ggaagtattt ggctcatcta taaaattttt caaacaagat attcattaaa
     1861 aggctctaaa attcgattta agcgattttt ctttcttggc tgtatatttt ccttgataat
     1921 cgtttcaagc ggcttattcg gcgttttaga gggaaataag cgcgtttcta gaagtatttt
     1981 attaggaaat gtcactcaaa agtatgaatc agcgcgtgat aaaaagaaaa aagagcaagc
     2041 attagcacaa aaaatggaag agtttaccac ttgttatgag gaaatgaacg atatttttgt
     2101 gaatcaagaa aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa
     2161 cttgccagaa aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt
     2221 gcaatatgta aaagatacaa agatagaaga agcctgtagt gatctatttg gagacacaaa
     2281 cccttggttt gccagtgaag aagaaaaaaa agaaaaacaa caatctgtca catatgaacg
     2341 ctatgaaatc ttacttcaac aagcaactaa catgcaaaat ccgacaaaaa aagaaaccgc
     2401 cttaaactat ctaaaaagtg tcaaggaatg gttagatcag caacaacaaa attgaatttg
     2461 atttaaaatc atgttaaact gaatgtgcaa atgatcaaac attagataca cgaaagcccc
     2521 agagaggtcg gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact
     2581 tcttgtttcg gttattcaac caataactga ataaagagac acaaactcct acaagaaggg
     2641 ggcaaatgaa cagttcaaac attagataca cctccttcca gtcgcaagac tgtcggatag
     2701 gcgacaagtt attatatctt aaaaaattaa atttgtgtgg tgtttttaag tatcacttgt
     2761 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg cttctcgact agattaaggg
     2821 aaattaatca agaattgtct tagaacggct tagacaagct ttcagagtct cacacctcct
     2881 ctttgaacaa cggttttagc aaaggagccg gaggcgactg cgcagtataa tatgagagca
     2941 tagcgatcac acctttttag cgaccgtagg gagcaagttt tccagtttag aaaaattcgc
     3001 gaaccttttt ggaggaaaaa gtaggagttt tttaggcggg aaattcaatg tgctcttaag
     3061 atatactgtt agatatactg ttagatatac tgttaataat aactgttagg gattagcaac
     3121 cttatgggag agtaagtatt gacataggta gtgggggata aattgtcaca ctatggggga
     3181 taaattgtca cactatgggg gataaattgt cacactatgg gggataaatt gtcacactat
     3241 gggggataaa ttgtgtttta catcctctta tgtctatgat aacataattt attgaggtga
     3301 tttttattga gtaacgatat agttcgatat aataatggat taaattctgt ccctttaaga
     3361 aaatttaccc ctatagatat ggatttattt tggggaatat gttctaaaat gaaaagaaaa
     3421 ggtacaaaag aagaaatttt tacatttaaa caaataaaag aattaattga ttacaatata
     3481 aatgcacgtg gcgaccgttt tgctaatgat ttaataaaaa tgtcaaaaaa aatcattact
     3541 ttgtctttta tttatgaaga cgaaaaaaca tatgaacaat taaatctgtt tcaaaaattt
     3601 aaaatcgata aagtgaatga aacattatta ataaaggttt ctgaagaatt tgaatatata
     3661 cttaattcaa tcggaactaa ttttactcgt ttcgaattag aaaatatgac aaacttaaag
     3721 agtagctacg ttaaagagtt atatcgccaa cttatggctc ataaagacag aagatctaaa
     3781 aaaggtcact ggtttgtaaa aatagaagat tttagagctc tattagcaat accagaaagt
     3841 tatcgcatga gagatattga taaacaaatt tttgcccaag cgaaaaaaga atttttaaac
     3901 gttaattcaa ctcaccctgc tatttttaaa acttttgaaa tcaaaaaaat taaagcctac
     3961 aaaggaaata agataggatc tctgcaaatt tattttgaag aaaatatacc ttcaattacc
     4021 ttagataatt ggttagagaa ataataccta acaatttagg aggcaattat catgcaaccc
     4081 aaagaaacct ttacaataaa ggaattatct gaattatttg aaataagtag gcaagcaatc
     4141 tctaaacatg ttgataaatt agacccgtcc ttattagcta aaaatgagcg tggttataag
     4201 gttgtattac gttctggagt attgcaactt gctagcaact taggtaacca aaggttacta
     4261 tataggttgc atgagtatga acaagaaaac gataaaaacg tttatatatc aagcaaaaac
     4321 gtgattgtaa acgagttgct agataagttg cgtgaagaaa atgaattttt acgagaacaa
     4381 ctttccctca aagagcaaaa taattctaat atgcaaaaat tactcgacca gcaacaacag
     4441 ctcaccctac aggcaaatca acagatcgaa aaactgcaag aacagttaca attagcttat
     4501 acggaagaaa ctccagaaga tgaatcaact actattcttt ctgaagaaac agaatcgata
     4561 gaaaaacaca caaaaaagaa gaagcaaaaa tggtggcaac tttggaaata actggaggga
     4621 acattcatga caagaaaaaa tcttctattc ggttctattt tactaggaat tctaggcttc
     4681 agtccgatgt tattgtcagt aatcaataaa gggaataaac atggtgaatt gaaattgaat
     4741 ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa aagagacgga taaataataa
     4801 aaaagactgc tcttccgagc agccagttaa aaaataatga gtgtttattc ttgaaaaagc
     4861 acgcgcttat gaagcgctgc caaaaattta ccatatttta caaaaaataa caagtcttaa
     4921 aaaaatgaca gataaaaatg cacaaagaat tagatataga taaccgcgaa aaatcaagct
     4981 ttagaaagtt gcttttagct agaccaatta aaaatcacaa agtgagcagc tatagccctt
     5041 gatagagggg ctacttggca tagtcgacat tatgtcaact atgcaattaa ataactaact
     5101 acttcatatt agcttaataa aataatcaaa ataactaggg ggaaaaacat tgtataaaaa
     5161 aatttttcat tttttaaata agaatagtaa atataaaaat aatttaagat atctattact
     5221 attaatccag atagagcaaa ccaagtaaat attccatgtg taaagctaaa aaaattcatt
     5281 tgaatcaatt caaataaacc taatatagta acaaatatat taaaaataat agctgtcaat
     5341 accagtaaat tatttttttt attttttgaa ttatcttcat ttaaaataga atttatgtta
     5401 gtactataaa attcacaaat tttttttagt gtctctatat ctggtgttgt tttgttattt
     5461 tcccactttg aaatagtttg acgtgtaacg ttaaattttc ttgccacata ttcttgagta
     5521 tacccttttt tcactcttaa ttctcttagc ttagatccta aatcctccat tttttctcct
     5581 atgtaaacga ttatttacat aaaattttat cataaaaata ctaacgcgcc aaaaaagttc
     5641 tttattttat aaaacacact aaaataaaat aattctagta aaaaagatag gagaaataag
     5701 ttatgaaaaa atttttatgt ctttctgctt taatgggtgt cttattaact agtggaggaa
     5761 tagctagtgc cacagaagct ttaaatctag atgtacccga agaccatacc gccatttacg
     5821 gaggaggaat ggaagctaca gaagaaggat tttcttctag taaattgaga tatgctgctg
     5881 gcggcgggga tttcaactgt ggggttaatg gatttaaagt ttatgcaaat tactaccatg
     5941 ctagagctaa acatagtgca acagctaaaa atggacgagg cggtcaagta cgatcagttc
     6001 agaaagctgg agtaagagct tatgcaacat gcaatgccac acttactgga aatactggtt
     6061 ggtggaatgt ttattaaaaa agagagttta aactctcttt tttatttaca tttgaaagga
     6121 aattcaaaat atgttaaaaa aaataaacgc tgttttatca ctttttgtct gcattacaat
     6181 actatttttt tctactaatg ttataaatgg tataaagact tctgaagcgc tatataaaaa
     6241 taccgctgaa attcatatgc aatataaaga tgatatggac tttaacagtt atataaacgc
     6301 tttgtatgaa atatctacac aagaaaatat taatatttct caatatagtt ttactgctgt
     6361 taaccaacta actattcttg ctacaaaccc taacgctaac aaacactgga aattttctaa
     6421 aaaaaatata ttagcaagaa acgattctaa agtccattat actaataaca aaaattctaa
     6481 gtaccaatta aaacttccaa ataatttttt aaatataaat atttatcctt tttctcatgt
     6541 ggaaaatatt ggattaagtg aaatattata tgtacaagga aactcaaaca aattaattcc
     6601 tgtactgaaa aaatatggaa cacctactat ttccaatatc aatcaatcag atacttttca
     6661 aattaatact aatattgttc ttgttatctg ctatcttagt atatttattg taattactac
     6721 aattatattt gctttttcaa aaatgaaaga aatcactcta aaaaaaatgt taggctataa
     6781 ctcttttgat actataaaag attctattaa agagttaaac ctcttatttt gttctttgat
     6841 tataactttc attttatcta tttttttgag catatattac agtaacgaaa attttttcct
     6901 atcatttctt ctatgtagta tttttcattt tataattagt tttttattta tcatagttac
     6961 tctaagtatc tatcatttat ctagtataaa aaattatcta aaaaattcac gaccacttaa
     7021 gttaaatctt tgtatattaa atatctgtct attcttaagc atgatccttc tgttaatagc
     7081 ttctacaaaa gtaataaata cccataatga agccgaaaat aattctctta aatattggga
     7141 aagaactact aatttataca aaacaaatat tactaatcaa ttaaatagaa acaacaccgt
     7201 agaagaaaat aactacttga aaaaagctag taaatttgtt tataaaatac agaagaatta
     7261 taaaactttt attatcgcgc catacaatta cgcaactatc caagagaata ataaagaatt
     7321 ttttattgga caaaaagttt atccaaattt tgaagactat gttagtcaac ctgctggtgc
     7381 aggaatatgt gttgatttaa actatctaaa atattacaat cctatttctt ttgaagaatc
     7441 tactgactta aatttaataa actctgatcc actaactact tacattctag tacctaaaaa
     7501 atataaagaa tatgcaaaaa tgattgaaaa aaattattta gaagacatac agtttagact
     7561 tgaggactct cctcctcaag ctccttataa aaatcctaat ttgaaaaatt taaaaataaa
     7621 actaatattt gttaataata accaaaagta ttttagtttt aataccttat atggaaaggc
     7681 aaaagataac tatacaataa ctgatcccat agttagagtt attaatccag aaataacaga
     7741 atcattattt tggggaaata tattgactag tgatggtgga ttattttttg atcagaaaca
     7801 tacttcaaac caaaactttt ttaatcaaat taatccgtat ataaaagaat ttaacctaga
     7861 aaatatcata aatttttctt attctgtttt tcaagaaact ggtgaattat tatctcaaaa
     7921 aaaagtaaat aaattaaatg catatctaca atatctttta atattaggat tatttataat
     7981 aattaatttt caaatgattt ttgttagttt tactataaat tcaaaaaaaa tagctttaaa
     8041 aaaaatattt ggatattcac caataagtca agttttagat ttatgtttac tcccttttac
     8101 aatagagtta tttgctacta tgtgttttca attgatattt tcaaaaaaca cttacttgta
     8161 ttttataatt tttatgatct ttttaataaa tctaattact tctattatta tttataaaaa
     8221 aaatagtttt aaatatttta aggagaatat tttatgatat tatcagttga gaatttcacc
     8281 ctgtcaatta ataaaaaaaa gatcaaacaa aattggaact tttcaatatc agaaaaagat
     8341 tttattatag taacaggaaa aagtggtagt ggaaaaacta ctctgttaaa taatttaagc
     8401 ctaatagata aacaatatac cggtaactta ttctattttg aaaagaaagt ttctaaacat
     8461 gatatacaac gtctacgaag aaatcatatt agctatttat ttcaaaacta tgctcttctt
     8521 gaaaatcaaa cagtacaata caatttcgaa ttagcaaaaa agtttaacag taatatttcc
     8581 aatgatcata tttataattt attaacagat ttttctttgc caaaagatat cttaaaagaa
     8641 aaaatctttc tcttaagcgg tggagaacaa caaagaatag cattaattag aaatatgtta
     8701 aaaccatttg atatattatt tgcagatgaa cctacaggaa atttagataa agaaaactca
     8761 acttttataa tgaactttct gaaaaattta acagaaaaag aaaaaaaagc cgttgtacta
     8821 gtaacacatg acttaaatgt tctaaactat ggaactaata ttatagaatt ataattttta
     8881 cccgtgatgc tagctgattt aaaactagca ccacgggtat ttattttata ttccatctct
     8941 cctatcccaa aatttaaata atcacttaat aaaaaggagg cacccaagtg attttattga
     9001 aaaaagagta tgaaaaattt tatgatagac ttatttattc tttgcaattg ccaaaaaaat
     9061 taaaccgtca attacaaaag acttatgaaa ctaaatcaac tgaagaatgg gagagttatt
     9121 gctagatttt tggtagaagt aacaaaaagt ggacatatta tcataatgac tcgccgaaag
     9181 tctgataaaa aattggctaa aaatattgct gaatgtttaa taaaactatc agttttagct
     9241 aaatagttag tagtttacac aaaaaagcat ttatgttttt tgttgcaaag taacaatttc
     9301 atcttcggtg aaataag
//
