LOCUS       NZ_LR135399             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8290 plasmid 6.
ACCESSION   NZ_LR135399
VERSION     NZ_LR135399.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885694
            Assembly: GCF_900639605.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:03:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,222
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 29 of 107
            Pseudo Genes (incomplete)         :: 75 of 107
            Pseudo Genes (internal stop)      :: 21 of 107
            Pseudo Genes (multiple problems)  :: 14 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135399.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8290"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            complement(209..928)
                     /locus_tag="EQB51_RS16780"
     CDS             complement(209..928)
                     /locus_tag="EQB51_RS16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            1612..1698
                     /locus_tag="EQB51_RS16785"
     CDS             1612..1698
                     /locus_tag="EQB51_RS16785"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_125669474.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2000..2224)
                     /locus_tag="EQB51_RS16790"
     CDS             complement(2000..2224)
                     /locus_tag="EQB51_RS16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            2590..2874
                     /locus_tag="EQB51_RS16795"
     CDS             2590..2874
                     /locus_tag="EQB51_RS16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            2877..>3221
                     /locus_tag="EQB51_RS16800"
     CDS             2877..>3221
                     /locus_tag="EQB51_RS16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127822614.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPK"
                     /name="hypothetical protein"
     Typing          complement(210..928)
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatcatcaaa aagaaaccag tcaaacagtc acttctagaa atttcgaccc aacaaatttt
       61 ctaaaacgat aaaaaacaga taggagttat tcctatctgt tttttattta gaagccccat
      121 acaatatgct agaatgtact agcaatctta gtcggttgta agggacttgt ctacttgctt
      181 aatgtaaggg gggcgagttc ttttttattt attcttctaa ccagttacgc agagaaatct
      241 ttggcaaatc tgacgttagg cttgaaaaag taaattctaa tttggcgatt ttattgccct
      301 tttttgcttt tatttttgtg actttaagat cattgaagta tttttttagt tctcgtaatg
      361 caggatttaa tactttttga tcaatgttac ccatttgata gtattctggg atatccatta
      421 agctcctaaa ctcttcaata cgaactacat aataaccagt agaacggtac tgcataagca
      481 gtcggaatag agtttttgca tacgtagaac gcaacgacgt gaaagcagat aattcaaatc
      541 tactaaattc agctgttaaa ccattaataa tatattctag gtccggatta acactgactt
      601 caacaaactt ttttgtttta ttgatttcaa aaccagtaaa caaaacaaag tagcgaatag
      661 aatcctcatc ttcagtacgg taagtaagat taagcatatc tttatagagc ttttctaaat
      721 caccgacaaa ggcttttgta gcagtcatct tataatcact taactctttt aattcctcga
      781 aagaaaatct aacttttttc aaaccacgat ctttcatacg agcgcataat gcaaaaaata
      841 aatccatctg cttagcattg aaatttttca gcggtactaa attcaattca ttttgatatt
      901 tgactgttaa ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa
      961 cctttaacaa ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc
     1021 agataaaaag ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta
     1081 gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc ttctgtatcc
     1141 gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct ttaaaaaata
     1201 aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc cactttttaa
     1261 aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg tcgctgtaag
     1321 aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag gctccttcgc
     1381 caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta agccgttcta
     1441 agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg cggtcagggc
     1501 aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt tttattgtat
     1561 aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt cgtgttcact
     1621 aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta ctggttgaat
     1681 aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa agccccagag
     1741 aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa tcaaattata
     1801 acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg taattatttc
     1861 ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg atttaagacg
     1921 ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta gaagcgattt
     1981 tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat gcgtcaaaag
     2041 ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc aacgatcgtt
     2101 ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa tcgaacttga
     2161 ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa ggaaattttt
     2221 ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt ctttgccgtc
     2281 tatactctca aaaaaaatgt cgtttgaaaa actaaataaa tttttgatcg tgcattttta
     2341 aatttggtta tcgattacac gtgcagcact cccccccgaa ttttaaagag cataaggaaa
     2401 cgtgtaccca ctttctaaaa aaagtgggta gataggctgt gcctatcacg tttctcagaa
     2461 aaaaatttga tatcactttt tcgccatcgt gggtagctta tgtatagcat tttggcaaaa
     2521 aacagctata caaatatagt atttgtatgg tatacataaa ttatacataa ttgcaaaagg
     2581 agatttttta tggacatttt agttcggaat attgatccaa agtttgttgc aacaatagac
     2641 aaacgttgcc aagaactcac gaataaaaca ggagaaaaat ggagtcgaaa taaatatcta
     2701 aaagttttaa tcgaaagtga ttttgaccgt caacttacga cttacaaaaa agaccaattc
     2761 gatttattgc tcgataaatt tattgctatc caaacagaaa atactcaaat attaagcgag
     2821 tatgtctaca caaataatca aatcattgaa atgcttatcg gaggaatgat ataaccatga
     2881 gaaaaacttc tgtccaaaaa catgttactt tgtaccctag tcatgttgac gttttagatt
     2941 ccatcatcga aaaaaatcca gatatcaata actactcgga agccattcgc tttttatgta
     3001 tgagttatga acccgatacc aacacgacca ctaccagcaa tcaggcaaaa ctaaatgcta
     3061 tgggaaaaga aatttccatc atgactgaaa tcatcgcaga atttgctgat ttatatctaa
     3121 aagatacagg cgttcttatt ggcacaaatt ctaaagtata tcaagaagtg aaagaaaaaa
     3181 tcgaagctaa actaaatcca tcaaatcgaa atcgaccaaa g
//
