LOCUS       NZ_LR135398             7837 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8290 plasmid 5.
ACCESSION   NZ_LR135398
VERSION     NZ_LR135398.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885694
            Assembly: GCF_900639605.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:03:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,222
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 29 of 107
            Pseudo Genes (incomplete)         :: 75 of 107
            Pseudo Genes (internal stop)      :: 21 of 107
            Pseudo Genes (multiple problems)  :: 14 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135398.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8290"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            1293..2261
                     /locus_tag="EQB51_RS16740"
     CDS             1293..2261
                     /locus_tag="EQB51_RS16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            2278..2802
                     /locus_tag="EQB51_RS16745"
     CDS             2278..2802
                     /locus_tag="EQB51_RS16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_044383356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            3178..3459
                     /locus_tag="EQB51_RS16750"
     CDS             3178..3459
                     /locus_tag="EQB51_RS16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            3461..3877
                     /locus_tag="EQB51_RS16755"
     CDS             3461..3877
                     /locus_tag="EQB51_RS16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            4210..4614
                     /gene="tnpA"
                     /locus_tag="EQB51_RS16760"
     CDS             4210..4614
                     /gene="tnpA"
                     /locus_tag="EQB51_RS16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            4631..5779
                     /gene="tnpB"
                     /locus_tag="EQB51_RS16765"
     CDS             4631..5779
                     /gene="tnpB"
                     /locus_tag="EQB51_RS16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_069722873.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADCYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            6444..7544
                     /locus_tag="EQB51_RS16770"
     CDS             6444..7544
                     /locus_tag="EQB51_RS16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="WP_224198038.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="cysteine protease staphopain"
     gene            7517..>7837
                     /locus_tag="EQB51_RS16775"
     CDS             7517..>7837
                     /locus_tag="EQB51_RS16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232043857.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRS"
                     /name="hypothetical protein"
     Typing          1294..2257
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aataaaaaac aattagatta cacttttagt ttggattctc caaaaaagct cgtatttatt
       61 ttgacaacga atgatgggac agaagggaca gctaaaccaa ttgaattttt taaagctgat
      121 tgatgaatag ttgtttgata ttacaaaaca ataatttcac cgaaggtgaa acaagcgaaa
      181 ctttacaaaa agtggctagc atttgctagc cttttttgta ttgtggagcg tggtttttga
      241 gggggattca agggggagct tgcgcccctt tgacaagcgg ttatttgcac caaatacgta
      301 ggtatttggc tagcaaatat agagcttgcc aaacacttta gtgagttttg tagaaagtac
      361 ctttaaaggt actttcacat tacgaacgat acgtccgtag gacgtactga aagggtggga
      421 tttatgagcg aactaatgca aaatttggct agcaaaaaaa gttaaaaaat atatgctaat
      481 aatcgagcga aatgaacact ttttcaagtg agcgaatccg ccatgacaaa gctgaagcag
      541 tttatttcag ctttgtcatg gcgtgtgcct agacttttaa aaaagtcggc acacgagcaa
      601 atatctttat caagctctaa tttgcgtttt aagacgtttg aaacgtctaa gtatataaat
      661 atataagagt aggtttaaga gtgcttaaat cgaattttag agggcattca gacaacttta
      721 gggttgttag ggcttgccct gaccgcctgt aaggcgcttt aagaacaaga taagggattt
      781 atactaagac tgtcttaaaa cgtctcaaac gaaggctcat agcttctcaa agagttcctt
      841 atgaacattt ttagcaaagg agccggaagc gactgcgcag tataatgtga gagcagagcg
      901 atcacacctt tttaagcgac tgtagggagt aagattccca gttgagaaaa atgcgcgaat
      961 ctgtttggag aaaaaagaag tggtttttta ggcgggaaat tttgtccccc tcttttataa
     1021 tctcttttta aacctctttt taaacctctt taccgtcggc tatatcctta ctctcccaag
     1081 ggaatgacgc ttttatctac atcgttttgt ctgtttatct acatcgtttt gtctgtttat
     1141 ctacatcgtt ttgtctgttt atctacatcg ttttgtctgt ttgtacattg aattagtaca
     1201 tcgacgatgt taatataaga tgtagataaa caggcatttt gtatttcata acgtgtatct
     1261 cttgtgcgag taagaaagga gctattttaa atatgtctaa tgaaatcgta aagtatcatc
     1321 atgaattaaa tacaattccg ttgcgaaaat ttacaccagt cgaaatgaat ttgttctttt
     1381 ctatcgtatc aagaatgcgt gatgtaggtg ataaaaaagt acaatttaca ttcgagcaat
     1441 taaaagattt aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa
     1501 caacgtatga caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac
     1561 gtgaacgttt tgttttattt aatcaattta aaatcgacgg aaaatctgat attccttttg
     1621 ctgaaataca agttcatgaa aaagccttac ccttacttaa taatcttgaa gaatgggtgc
     1681 gctattctct ccaacaattc aatgaattag aaagcagtta ttcaaaaaca atgtttcgat
     1741 tactaaaaca atacagaacc aaaggtttcg cttatttttc aaaagaagac tttcatgaat
     1801 tacttgatat tcctaaaagt tacaaccaag gtacgattga tcaacgtgtg ttcaatccaa
     1861 tacgacaaga actaaccgca atctttaaag gtttaacaat caaaaagaaa tacggaaaag
     1921 gacggggaaa gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag
     1981 acgactttaa caaacatgcc ccaacaaaag aagaacaaaa caaagcatta gaactagcta
     2041 accaacaagt tcaatctgaa aaagcaccta cttcattaca agaaaaaatc aaagaacaaa
     2101 agcgaaaatt ggaactttta caacagttag aaaagttaga aagagaacaa aatacccaaa
     2161 ataaagaaaa taatgcacaa aaatcagaga taaattccga tattccttca gaactaaaaa
     2221 aagacttatt agacaatctt caaaacttat tcaaagattg aggggttttt agattttatg
     2281 acagaaaaaa caatcaaaga acttgctgaa gaattaggag ttagtaaaac agcaattaac
     2341 aaaaaagttt ctgacgaaaa tcgaaaactt tggttttcga aaatcggaaa caagtttgtg
     2401 attaatgaaa ccggacaaaa ggctataaaa tcaatgtttt ttaacgaaaa cgaaaactca
     2461 aatcaacaac tagttggcga aaaaacgaaa accgaaaacc acaaaaacgc caaccaagtt
     2521 cccgaattag aagtcctttc tctcctagaa caacagttaa atcaaaaaga tatgcaacta
     2581 caagaaaaag acgagcaaat aaaacaaatg caaaaattac tcgaccacca acaacaactc
     2641 accctgcaag ccaaccaaca aatcgagaaa ctgcaggaac aactacagtt gacttatacg
     2701 gaagaatctt cggaaaataa atcaacgact ctttctgaag aaacagaatc gatagaaaaa
     2761 caatcaaagg cggagaaaaa atggtggcaa ttctggcaat aaaaccagca atttaaatta
     2821 ataaaaattg ctggttttat tcttcttttt gcttttgcga gcagagcaac atggacccat
     2881 gttgcgatat tttggtggcg ccccaatccc cgttttttcg ttgaatctta ctcggactgc
     2941 actcgtttta cagctgattt ttctgaattt tgaaagagca taaggaaacg tggtatcact
     3001 ttcttacgaa aggtgctaga taggctgtgc ctatcacgtt tctcagaaaa aaatttgcta
     3061 tcactttttt gccattgtgg ctaggctatg tatagtgttt tggcgaaaaa tagctataca
     3121 aatactatat ttgtatggta tacataaatt atacgctgat acgaaaggaa aatttccatg
     3181 gacattttag ttcgaaatat caatcccaaa attattgcaa gaatagataa acgttgtcag
     3241 gaactcacaa ccaaaacggg caaaaaatgg agtcgaaata aatatctaaa agttctaatc
     3301 gaaagtgatt ttgaccgtca acttatgaat tacaaaaaag atcaattcga tttattactc
     3361 gaaaaattta ttgccattca aacagaaaat acgaacatac tgaatgacta tgttcacaca
     3421 aacaaccaac tcattgaaac acttatcgga ggaatataac atgagaaaaa cttctgttca
     3481 aaaacacgta accctttatc ccagtcacgt tgacgtttta gattccatca tcgaaaaaaa
     3541 tccagacgtc aacaattatt cagaagccat tcgtttttta tgtatgagtt atgaacctga
     3601 taccaataca accacaacca gtaaccaagc aaaactcaat gccatgggaa aagaaatttc
     3661 tatcatgaca gaaatcatgg cagaatttgc tgatttatat ctaaaagata ctggtgttct
     3721 tatcggtaca aattctaaag tgtatcaaga agtgaaagaa aaaattgaaa atcaactcaa
     3781 tccttcccga cgaaatcaac ccaaaaatcg tcaaaaagaa accaatcaaa cctttacttc
     3841 tggaaatttt gacccaacaa actttctaaa aagataaaaa aagaaaccga tccctaaaaa
     3901 aattcctcga tttcttttta gggatcagtc ttccagcaag atttgcaagt tggtaccaac
     3961 ttgcgaaaaa aaagaaaaat ccgcttatgc aacttcttct ttttgtattt tggggcgcta
     4021 ccccaatccc caagtatcgg ttatttaaca gaattaggca gaagtcgccc atcctctata
     4081 ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg gtaactcgaa gaccgacaac
     4141 taaggcgtat tgccttttta ttttgttcca tgttacaatc agtctataca agaaatgagt
     4201 tgatataaca tggaattaga tacaaataat cattcagtat ttcttcttta ttaccacctt
     4261 gttttagtaa cgaaatatcg tagacaagtg attgatgatg aaatatctga ttatgccaaa
     4321 actacttttg aaagaatttc agaatcgtat cacattactt tagtggagtg gaatcatgat
     4381 aaagaccacg ttcatattat gttcaaagct caacctaaaa cagaattgac aaaattcatc
     4441 aatgcctata aaagtgccag ctcaagattg ataaaacgtg actttccaag agtcaaacaa
     4501 tttctttgga aagaaatgtt ctggtctaaa agtttttgtc ttctgacaac tggtggcgca
     4561 ccaattgatg tgattaagaa gtatatccaa aatcaaggaa acaatcataa atagaaagta
     4621 ggtgaacttt atggaacaac taaaagcata caaattcaga atctatccaa cagaggaaca
     4681 agaaatcttc tttgccaaat cttttggttg tgtccgtaag gtctacaatc taatgcttga
     4741 tgaccgaaaa aaggcgtatg aagaagttaa aaatgattct tctaaaaaaa tgacttttcc
     4801 gacaccagct aaatacaaga aagaatttcc gtttttgaaa gaaattgata gccttgcttt
     4861 agccaatgcc caacttaatt tagataaagc atacaagaat ttttttcggg ataaatccgt
     4921 tggatttcca cgtttcaaaa gtaagaaaaa tcctgttcag agttatacaa cgaataatca
     4981 aaatggtaca gttgctttga ttgatagtaa attcatcaaa gttcctaaat tgaagtcatt
     5041 agtaagaatt aagcttcata gacaaccaaa agggattatc aaatccgcta caatatcacg
     5101 tcattctagc ggtaaatact atatttcttt gttgtgtaaa gaagaaatta tcgaattacc
     5161 taaaactaat tccacaattg gtattgactt aggtattatg gactttgcga ttctttctga
     5221 tggacaaaaa attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga
     5281 acaacgcaaa ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga
     5341 agctaaaaat tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa
     5401 tcaacgtact gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat
     5461 ctgtattgaa gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat
     5521 ttctgatgtt tcttggtcaa agtttgtgac taaattacaa tacaaggctg actgttatgg
     5581 acgaaaaatc atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg
     5641 acataaagat ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac
     5701 tcatcatgat cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca
     5761 ttctatggga ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg
     5821 gtaaataaga gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa
     5881 gaagctccca cttcaagcgt taagaaccgt caggtttagc taagtggtga gtagttcact
     5941 agcgcggaaa tccaaagaat aggacgtacc ctgaaacaaa aaaagactgc tctcacgagc
     6001 agcccaaaat gagattatcc tattcatcaa cataaaaata ttacaaagtg aagaactaga
     6061 gaaagacaaa aaaagcatat caaatcctct caaaaataac aagtctttaa aaaatgacag
     6121 ataaaaatgc acaaagaact aaaaaagaga aatcgcgtaa aatcaagcat tagaagctag
     6181 taactagcta gaccaattaa aaatcacaaa gtgagcgtct ctatccattg gtagaggggc
     6241 tacttggcat agtcgacatt atgttaacta acgctataaa taacgattat gttcccaaaa
     6301 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
     6361 tgtataccac ttaaatttaa cacaaaggcg tgattatttt gaaacccaaa aagaaaattt
     6421 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
     6481 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
     6541 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
     6601 ccgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
     6661 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatata
     6721 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
     6781 caaaagaact tcaaaatcta gtagatagta atataacaga tacaacctta ttttttcaag
     6841 ataatagcat ttattattcc gataatggac aaattaaact actttggaaa tcacctttaa
     6901 gtacagaaaa aaatggtgtt tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     6961 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     7021 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     7081 caagttttcc gtggtgtgct gcttacgtag aagctgctat cttatgtaac aaaacagatg
     7141 tacgtgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     7201 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     7261 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     7321 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     7381 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     7441 cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca attcctgtac
     7501 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgcttt
     7561 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     7621 aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt cagaacgtct
     7681 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     7741 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     7801 ttacaaagca acaaataatt tactaacact gcgctct
//
