LOCUS       NZ_LR135397             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8290 plasmid 4.
ACCESSION   NZ_LR135397
VERSION     NZ_LR135397.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885694
            Assembly: GCF_900639605.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:03:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,222
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 29 of 107
            Pseudo Genes (incomplete)         :: 75 of 107
            Pseudo Genes (internal stop)      :: 21 of 107
            Pseudo Genes (multiple problems)  :: 14 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8290"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            complement(<1..179)
                     /locus_tag="EQB51_RS16675"
     CDS             complement(<1..179)
                     /locus_tag="EQB51_RS16675"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_145987361.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            312..686
                     /locus_tag="EQB51_RS16680"
     CDS             312..686
                     /locus_tag="EQB51_RS16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            740..2866
                     /locus_tag="EQB51_RS16685"
     CDS             740..2866
                     /locus_tag="EQB51_RS16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            2863..3483
                     /locus_tag="EQB51_RS16690"
     CDS             2863..3483
                     /locus_tag="EQB51_RS16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            3597..3734
                     /locus_tag="EQB51_RS17020"
     CDS             3597..3734
                     /locus_tag="EQB51_RS17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            4176..4556
                     /gene="mobC"
                     /locus_tag="EQB51_RS16695"
     CDS             4176..4556
                     /gene="mobC"
                     /locus_tag="EQB51_RS16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            4538..5452
                     /locus_tag="EQB51_RS16700"
     CDS             4538..5452
                     /locus_tag="EQB51_RS16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5412..5690
                     /locus_tag="EQB51_RS17375"
     CDS             5412..5690
                     /locus_tag="EQB51_RS17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            5692..6381
                     /locus_tag="EQB51_RS16710"
     CDS             5692..6381
                     /locus_tag="EQB51_RS16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6502..6588)
                     /locus_tag="EQB51_RS16715"
     CDS             complement(6502..6588)
                     /locus_tag="EQB51_RS16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            7233..7970
                     /locus_tag="EQB51_RS16720"
     CDS             7233..7970
                     /locus_tag="EQB51_RS16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            7998..8537
                     /locus_tag="EQB51_RS16725"
     CDS             7998..8537
                     /locus_tag="EQB51_RS16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            8553..8723
                     /locus_tag="EQB51_RS17025"
     CDS             8553..8723
                     /locus_tag="EQB51_RS17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(9035..>9317)
                     /locus_tag="EQB51_RS17240"
                     /pseudo=""
     CDS             complement(9035..>9317)
                     /locus_tag="EQB51_RS17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /name="XRE family transcriptional regulator"
     BGC             1..5686
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7234..7970
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4539..5449
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatgttag tactataaaa ttcacaaatt ttttttagtg tctctatatc tggtgttgtt
       61 ttgttatttt cccactttga aatagtttga cgtgtaacgt taaattttct tgccacatat
      121 tcttgagtat accctttttt cactcttaat tctcttagct tagatcctaa atcctccatt
      181 ttttctccta tgtaaacgat tatttacata aaattttatc ataaaaatac taacgcgcca
      241 aaaaagttct ttattttata aaacacacta aaataaaata attctagtaa aaaagatagg
      301 agaaataagt tatgaaaaaa tttttatgtc tttctgcttt aatgggtgtc ttattaacta
      361 gtggaggaat agctagtgcc acagaagctt taaatctaga tgtacccgaa gaccataccg
      421 ccatttacgg aggaggaatg gaagctacag aagaaggatt ttcttctagt aaattgagat
      481 atgctgctgg cggcggggat ttcaactgtg gggttaatgg atttaaagtt tatgcaaatt
      541 actaccatgc tagagctaaa catagtgcaa cagctaaaaa tggacgaggc ggtcaagtac
      601 gatcagttca gaaagctgga gtaagagctt atgcaacatg caatgccaca cttactggaa
      661 atactggttg gtggaatgtt tattaaaaaa gagagtttaa actctctttt ttatttacat
      721 ttgaaaggaa attcaaaata tgttaaaaaa aataaacgct gttttatcac tttttgtctg
      781 cattacaata ctattttttt ctactaatgt tataaatggt ataaagactt ctgaagcgct
      841 atataaaaat accgctgaaa ttcatatgca atataaagat gatatggact ttaacagtta
      901 tataaacgct ttgtatgaaa tatctacaca agaaaatatt aatatttctc aatatagttt
      961 tactgctgtt aaccaactaa ctattcttgc tacaaaccct aacgctaaca aacactggaa
     1021 attttctaaa aaaaatatat tagcaagaaa cgattctaaa gtccattata ctaataacaa
     1081 aaattctaag taccaattaa aacttccaaa taatttttta aatataaata tttatccttt
     1141 ttctcatgtg gaaaatattg gattaagtga aatattatat gtacaaggaa actcaaacaa
     1201 attaattcct gtactgaaaa aatatggaac acctactatt tccaatatca atcaatcaga
     1261 tacttttcaa attaatacta atattgttct tgttatctgc tatcttagta tatttattgt
     1321 aattactaca attatatttg ctttttcaaa aatgaaagaa atcactctaa aaaaaatgtt
     1381 aggctataac tcttttgata ctataaaaga ttctattaaa gagttaaacc tcttattttg
     1441 ttctttgatt ataactttca ttttatctat ttttttgagc atatattaca gtaacgaaaa
     1501 ttttttccta tcatttcttc tatgtagtat ttttcatttt ataattagtt ttttatttat
     1561 catagttact ctaagtatct atcatttatc tagtataaaa aattatctaa aaaattcacg
     1621 accacttaag ttaaatcttt gtatattaaa tatctgtcta ttcttaagca tgatccttct
     1681 gttaatagct tctacaaaag taataaatac ccataatgaa gccgaaaata attctcttaa
     1741 atattgggaa agaactacta atttatacaa aacaaatatt actaatcaat taaatagaaa
     1801 caacaccgta gaagaaaata actacttgaa aaaagctagt aaatttgttt ataaaataca
     1861 gaagaattat aaaactttta ttatcgcgcc atacaattac gcaactatcc aagagaataa
     1921 taaagaattt tttattggac aaaaagttta tccaaatttt gaagactatg ttagtcaacc
     1981 tgctggtgca ggaatatgtg ttgatttaaa ctatctaaaa tattacaatc ctatttcttt
     2041 tgaagaatct actgacttaa atttaataaa ctctgatcca ctaactactt acattctagt
     2101 acctaaaaaa tataaagaat atgcaaaaat gattgaaaaa aattatttag aagacataca
     2161 gtttagactt gaggactctc ctcctcaagc tccttataaa aatcctaatt tgaaaaattt
     2221 aaaaataaaa ctaatatttg ttaataataa ccaaaagtat tttagtttta ataccttata
     2281 tggaaaggca aaagataact atacaataac tgatcccata gttagagtta ttaatccaga
     2341 aataacagaa tcattatttt ggggaaatat attgactagt gatggtggat tattttttga
     2401 tcagaaacat acttcaaacc aaaacttttt taatcaaatt aatccgtata taaaagaatt
     2461 taacctagaa aatatcataa atttttctta ttctgttttt caagaaactg gtgaattatt
     2521 atctcaaaaa aaagtaaata aattaaatgc atatctacaa tatcttttaa tattaggatt
     2581 atttataata attaattttc aaatgatttt tgttagtttt actataaatt caaaaaaaat
     2641 agctttaaaa aaaatatttg gatattcacc aataagtcaa gttttagatt tatgtttact
     2701 cccttttaca atagagttat ttgctactat gtgttttcaa ttgatatttt caaaaaacac
     2761 ttacttgtat tttataattt ttatgatctt tttaataaat ctaattactt ctattattat
     2821 ttataaaaaa aatagtttta aatattttaa ggagaatatt ttatgatatt atcagttgag
     2881 aatttcaccc tgtcaattaa taaaaaaaag atcaaacaaa attggaactt ttcaatatca
     2941 gaaaaagatt ttattatagt aacaggaaaa agtggtagtg gaaaaactac tctgttaaat
     3001 aatttaagcc taatagataa acaatatacc ggtaacttat tctattttga aaagaaagtt
     3061 tctaaacatg atatacaacg tctacgaaga aatcatatta gctatttatt tcaaaactat
     3121 gctcttcttg aaaatcaaac agtacaatac aatttcgaat tagcaaaaaa gtttaacagt
     3181 aatatttcca atgatcatat ttataattta ttaacagatt tttctttgcc aaaagatatc
     3241 ttaaaagaaa aaatctttct cttaagcggt ggagaacaac aaagaatagc attaattaga
     3301 aatatgttaa aaccatttga tatattattt gcagatgaac ctacaggaaa tttagataaa
     3361 gaaaactcaa cttttataat gaactttctg aaaaatttaa cagaaaaaga aaaaaaagcc
     3421 gttgtactag taacacatga cttaaatgtt ctaaactatg gaactaatat tatagaatta
     3481 taatttttac ccgtgatgct agctgattta aaactagcac cacgggtatt tattttatat
     3541 tccatctctc ctatcccaaa atttaaataa tcacttaata aaaaggaggc acccaagtga
     3601 ttttattgaa aaaagagtat gaaaaatttt atgatagact tatttattct ttgcaattgc
     3661 caaaaaaatt aaaccgtcaa ttacaaaaga cttatgaaac taaatcaact gaagaatggg
     3721 agagttattg ctagattttt ggtagaagta acaaaaagtg gacatattat cataatgact
     3781 cgccgaaagt ctgataaaaa attggctaaa aatattgctg aatgtttaat aaaactatca
     3841 gttttagcta aatagttagt agtttacaca aaaaagcatt tatgtttttt gttgcaaagt
     3901 aacaatttca tcttcggtga aataagcgaa actttacaaa aagtggctag catttgctag
     3961 ctttttttgt attgtggagc gtggcttttg agggggattc aagggggagc ttgcgcccct
     4021 ttgacaagcg gttatttgca ctaaatacgt aggtatttgg ctagcaaata tagagcttgc
     4081 caaacccttt tagtgagctt tactcattgt acctttttag gtacaatggt attgcgaacg
     4141 atacgtccgt aggacgtact gaaagggtgg gacttatgag cgaacaaaat caaaatttgg
     4201 ctagcaattc ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc agttttcgag
     4261 tgagcgaatc cgaatttgca aagctgaagc aatcggctga agctttgcaa atgagtgtgc
     4321 cggctttcgt gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag
     4381 acattgccca ggcaatcgcg cgcgatttag ccaaagctgg agggaatatc aatcaaattg
     4441 ctaagtggtg caatacacac caacatgacg ttgcttctgg tgacgctcaa cgtttatccg
     4501 aaaacctaaa aatcatgcaa aaggagctac aaaagatatg gcaacaatta aggtaagccg
     4561 aaccacttct tgtagtcgtg caattaatta cgcagaacca cgagcaacca ttaaaacagg
     4621 aataaactgt gacgtcgatt atgccaaaag tgaaatgaaa caaattcgca tgctgtatgg
     4681 aaaagacgac cgtgtacagg cacacttatt gattcaatct tttcgaccag gagaaattac
     4741 cgctgaaaaa gctaatcaac taggggcaga gtatgcaaaa aaaatagccc ccgatcacca
     4801 aacagctatt tacacacata cagacaaaga ccatattcat aatcacattg tcattaactc
     4861 cgttaatctg gagactggaa aaaaatttca agctcatggt caagcatttc taaacaaatg
     4921 ttatgaccta aatgacgaga tttgtcttgc acataactta ccaatcactg agcgaggaaa
     4981 gaaaccagaa aaacgaacca tgtctgaaat aaaattaaaa gaaaaaaatg aacctgtttg
     5041 gaaagacgaa attcggattg cgatcgatca aacaatgaaa aacaaaaaaa ccagaaccta
     5101 tgaccaattt tgtaattccc tcaaaacatt ggggattcat tgcttcaatc gcgggaaaaa
     5161 tttcacttac gaattgatcc acaaaaagaa aaaagtacga tccaacagac taggaaagga
     5221 ctacgaaaag gagacgattt tacatgagct ggacagacga gaacgaacct ataaccaaca
     5281 gcgaaccaac gagctacgaa gagctcgagc tagcattcgc gaaactgcag aagaagtacg
     5341 aagagaacaa cgaactctta cgcctgaaca accggaaatt agaccggctc gaagaaacca
     5401 tgaacaacac gttgaaagag gaacagacca ccaacaagaa ttatcgctct aaaacgttaa
     5461 aagacataga aaacgaaata aagaaacaac gtgcttttct cgttccttat acgcaagaaa
     5521 ggatacaaac tacgattgat ttacaagtta aaaaactcca tcaagagcta ttagaccagc
     5581 gaaataagga acagaaaaag gtgcttattc aacgatcatt ggattttctt gcccccttat
     5641 gttgcttagt cctgacactg ttcgcatttt ggttagtatg gggaggctaa aatggcattt
     5701 tatttaccct atctactcat ttttgttagt atttcaggaa gtatttggct catctataaa
     5761 atttttcaaa caagatattc attaaaaggc tctaaaattc gatttaagcg atttttcttt
     5821 cttggctgta tattttcctt gataatcgtt tcaagcggct tattcggcgt tttagaggga
     5881 aataagcgcg tttctagaag tattttatta ggaaatgtca ctcaaaagta tgaatcagcg
     5941 cgtgataaaa agaaaaaaga gcaagcatta gcacaaaaaa tggaagagtt taccacttgt
     6001 tatgaggaaa tgaacgatat ttttgtgaat caagaaaaac gtttgacgga taaaaacatg
     6061 gaaaaactca ctcgtcttta tcaaaacttg ccagaaaaac agcaaaaaga ggttcaagac
     6121 aactatgagc gaaccaaaaa agacgtgcaa tatgtaaaag atacaaagat agaagaagcc
     6181 tgtagtgatc tatttggaga cacaaaccct tggtttgcca gtgaagaaga aaaaaaagaa
     6241 aaacaacaat ctgtcacata tgaacgctat gaaatcttac ttcaacaagc aactaacatg
     6301 caaaatccga caaaaaaaga aaccgcctta aactatctaa aaagtgtcaa ggaatggtta
     6361 gatcagcaac aacaaaattg aatttgattt aaaatcatgt taaactgaat gtgcaaatga
     6421 tcaaacatta gatacacgaa agccccagag aggtcggatt ctctggggct ttttttcagg
     6481 ttgatgtcgc ctttattgat tttacttctt gtttcggtta ttcaaccaat aactgaataa
     6541 agagacacaa actcctacaa gaagggggca aatgaacagt tcaaacatta gatacacctc
     6601 cttccagtcg caagactgtc ggataggcga caagttatta tatcttaaaa aattaaattt
     6661 gtgtggtgtt tttaagtatc acttgtttta gggttgttag ggcttgccct gaccgcctgt
     6721 aaggcgcttc tcgactagat taagggaaat taatcaagaa ttgtcttaga acggcttaga
     6781 caagctttca gagtctcaca cctcctcttt gaacaacggt tttagcaaag gagccggagg
     6841 cgactgcgca gtataatatg agagcatagc gatcacacct ttttagcgac cgtagggagc
     6901 aagttttcca gtttagaaaa attcgcgaac ctttttggag gaaaaagtag gagtttttta
     6961 ggcgggaaat tcaatgtgct cttaagatat actgttagat atactgttag atatactgtt
     7021 aataataact gttagggatt agcaacctta tgggagagta agtattgaca taggtagtgg
     7081 gggataaatt gtcacactat gggggataaa ttgtcacact atgggggata aattgtcaca
     7141 ctatggggga taaattgtca cactatgggg gataaattgt gttttacatc ctcttatgtc
     7201 tatgataaca taatttattg aggtgatttt tattgagtaa cgatatagtt cgatataata
     7261 atggattaaa ttctgtccct ttaagaaaat ttacccctat agatatggat ttattttggg
     7321 gaatatgttc taaaatgaaa agaaaaggta caaaagaaga aatttttaca tttaaacaaa
     7381 taaaagaatt aattgattac aatataaatg cacgtggcga ccgttttgct aatgatttaa
     7441 taaaaatgtc aaaaaaaatc attactttgt cttttattta tgaagacgaa aaaacatatg
     7501 aacaattaaa tctgtttcaa aaatttaaaa tcgataaagt gaatgaaaca ttattaataa
     7561 aggtttctga agaatttgaa tatatactta attcaatcgg aactaatttt actcgtttcg
     7621 aattagaaaa tatgacaaac ttaaagagta gctacgttaa agagttatat cgccaactta
     7681 tggctcataa agacagaaga tctaaaaaag gtcactggtt tgtaaaaata gaagatttta
     7741 gagctctatt agcaatacca gaaagttatc gcatgagaga tattgataaa caaatttttg
     7801 cccaagcgaa aaaagaattt ttaaacgtta attcaactca ccctgctatt tttaaaactt
     7861 ttgaaatcaa aaaaattaaa gcctacaaag gaaataagat aggatctctg caaatttatt
     7921 ttgaagaaaa tataccttca attaccttag ataattggtt agagaaataa tacctaacaa
     7981 tttaggaggc aattatcatg caacccaaag aaacctttac aataaaggaa ttatctgaat
     8041 tatttgaaat aagtaggcaa gcaatctcta aacatgttga taaattagac ccgtccttat
     8101 tagctaaaaa tgagcgtggt tataaggttg tattacgttc tggagtattg caacttgcta
     8161 gcaacttagg taaccaaagg ttactatata ggttgcatga gtatgaacaa gaaaacgata
     8221 aaaacgttta tatatcaagc aaaaacgtga ttgtaaacga gttgctagat aagttgcgtg
     8281 aagaaaatga atttttacga gaacaacttt ccctcaaaga gcaaaataat tctaatatgc
     8341 aaaaattact cgaccagcaa caacagctca ccctacaggc aaatcaacag atcgaaaaac
     8401 tgcaagaaca gttacaatta gcttatacgg aagaaactcc agaagatgaa tcaactacta
     8461 ttctttctga agaaacagaa tcgatagaaa aacacacaaa aaagaagaag caaaaatggt
     8521 ggcaactttg gaaataactg gagggaacat tcatgacaag aaaaaatctt ctattcggtt
     8581 ctattttact aggaattcta ggcttcagtc cgatgttatt gtcagtaatc aataaaggga
     8641 ataaacatgg tgaattgaaa ttgaatccaa attattacaa cagtcctaac gaagaattgt
     8701 ttgtaaaaga gacggataaa taataaaaaa gactgctctt ccgagcagcc agttaaaaaa
     8761 taatgagtgt ttattcttga aaaagcacgc gcttatgaag cgctgccaaa aatttaccat
     8821 attttacaaa aaataacaag tcttaaaaaa atgacagata aaaatgcaca aagaattaga
     8881 tatagataac cgcgaaaaat caagctttag aaagttgctt ttagctagac caattaaaaa
     8941 tcacaaagtg agcagctata gcccttgata gaggggctac ttggcatagt cgacattatg
     9001 tcaactatgc aattaaataa ctaactactt catattagct taataaaata atcaaaataa
     9061 ctagggggaa aaacattgta taaaaaaatt tttcattttt taaataagaa tagtaaatat
     9121 aaaaataatt taagatatct attactatta atccagatag agcaaaccaa gtaaatattc
     9181 catgtgtaaa gctaaaaaaa ttcatttgaa tcaattcaaa taaacctaat atagtaacaa
     9241 atatattaaa aataatagct gtcaatacca gtaaattatt ttttttattt tttgaattat
     9301 cttcatttaa aatagaa
//
