LOCUS       NZ_LR135393             2056 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7933 plasmid 10.
ACCESSION   NZ_LR135393
VERSION     NZ_LR135393.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885473
            Assembly: GCF_900639545.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:50:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,344
            CDSs (total)                      :: 3,251
            Genes (coding)                    :: 3,135
            CDSs (with protein)               :: 3,135
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 39 of 116
            Pseudo Genes (incomplete)         :: 75 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 14 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135393.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7933"
                     /db_xref="taxon:1352"
                     /plasmid="10"
     gene            246..467
                     /locus_tag="EQB43_RS17475"
     CDS             246..467
                     /locus_tag="EQB43_RS17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            468..725
                     /locus_tag="EQB43_RS17480"
     CDS             468..725
                     /locus_tag="EQB43_RS17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(861..1463)
                     /locus_tag="EQB43_RS17485"
     CDS             complement(861..1463)
                     /locus_tag="EQB43_RS17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1530..1685)
                     /locus_tag="EQB43_RS17490"
     CDS             complement(1530..1685)
                     /locus_tag="EQB43_RS17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     Typing          628..803
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(934..1463)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agtcgcctta caggctccat tctcgcttct cttacgtgtt ttaacgattg cgccgataac
       61 taaacgaaat agacgctaaa tcgcttaaaa cgcaaaatag acccttttta gagcattgat
      121 tttttctact agtcatttcg ataatataaa attgaccaca acacagaaaa cttatattat
      181 aagcttatgt cgtaagtccg acgtactcac gtaatataga cggacgttaa atggaaggag
      241 cataaatgag agtgataaaa tcagaaaaat tcactttggc cgaaattatg acgaccagcg
      301 aacttgcaca agaacttgat tacaaccaag cttacgtttt gcgcttagcg aaagaacatc
      361 tgactgaagg aaaagaatat cgctctgccg gtagaagaaa ctatcttttc agcccagaag
      421 cccttaaaaa actagaaaaa gtcataacca ccaaagaagg tgagtaaatg cctaaagaaa
      481 caactatcaa cgttgaacaa ctagaaaaat tgctagaatc atattccaat gattttatcg
      541 aattgaccgg cggaaaagtc gaagaaacca ccgacctctt tgacctactt ctcacaaata
      601 ttctggaatt aaactctgaa gaacaaaaag agcataaggg aaaactgata gcaccttcct
      661 acaggaaggt ggctaagtcg gctgtgccaa ctggttttct tacaaaacaa ccttatattt
      721 tttgatatca caaaaaaatc cattttggac ctattttcgg tcataatata gtacctactt
      781 ttggtcatag tttcgtccgt agttttggtc atagtgacta agactttcac gcacaaaaaa
      841 agagctgaaa ttaacagctc ttatccttta tttatttttg aatttacact tccaccatga
      901 cgattcgatt ttatatattg cccgataata taagctgaat tatcacaaag aagcggaaac
      961 caatcatcaa atctttctga cattgcatag tcaattaaat ctttgatttc agtgatattc
     1021 ttagaatcaa caaacgacat catctctcta atcaactcat atcgttctgc actcgttact
     1081 gacaaataac tagcaatttc tgcaccacca tgagcgataa tatcactttt agcatattga
     1141 aatttttctg gattatccat atgagcaaaa tatcgcacca taccttttgc attagcacat
     1201 ttttgcgggt ttggagaccg aagcttttga gttatctcac gtatttgatc atacgtcttt
     1261 ttaccgctaa acattatcaa aacatgccaa tgaggctttt tcaattcacc gtcaggattc
     1321 acatctttat catgaagtgg actctcaacc caaggaacat gtaactcatc taaaaactcc
     1381 ctccaatttt caggagccga ttcaggatag actacaaacg tccaattcct tgtgcgttca
     1441 tctttagttt ttctactcgt catgctataa ttaccttaat tgaacaacag tctaacagca
     1501 aaagcagtgc gccaacacta atgcttttgc tatttttttt gctcattttc ctttccgtta
     1561 gcattaaccc aaattgtaat aagaccagac ttactaagac ccatttgttt cgcaatttta
     1621 tcaagattat ctgccacttc cttagtaagc gttacaacga ctctcttttt ttcattactg
     1681 atcataattc gtatcctcca acgacttttt cttaaaaacc atagtaacaa aaaagtaact
     1741 aaggtgcaac agtttagata ctaattagcg aataataggc cttaaaccct tggcattact
     1801 gattttgtca aaaaaaaacg ctgaaaccct tgggagagta aggctaaaca gctatcgacc
     1861 aatgaccaat gaccaataag gggggtgtcg tagtcccccc ctcctccgcc caattcataa
     1921 acaccaaaac caaaaaacaa acctcagggc tccgccccga accccgacct ccttctaccg
     1981 aaatatatgc aggcgccacg cttactattt cgttcagcta tcggcttttg caatctaaca
     2041 cgaccgtttc gctacc
//
