LOCUS       NZ_LR135392             3221 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7933 plasmid 9.
ACCESSION   NZ_LR135392
VERSION     NZ_LR135392.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885473
            Assembly: GCF_900639545.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:50:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,344
            CDSs (total)                      :: 3,251
            Genes (coding)                    :: 3,135
            CDSs (with protein)               :: 3,135
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 39 of 116
            Pseudo Genes (incomplete)         :: 75 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 14 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135392.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7933"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            230..454
                     /locus_tag="EQB43_RS17450"
     CDS             230..454
                     /locus_tag="EQB43_RS17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000410576.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            1526..2245
                     /locus_tag="EQB43_RS17460"
     CDS             1526..2245
                     /locus_tag="EQB43_RS17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(2382..2798)
                     /locus_tag="EQB43_RS17465"
     CDS             complement(2382..2798)
                     /locus_tag="EQB43_RS17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2801..3085)
                     /locus_tag="EQB43_RS17470"
     CDS             complement(2801..3085)
                     /locus_tag="EQB43_RS17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     Typing          1527..2245
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaacgtgata ggcacagcct atctacccac tttttttaga aagtgggtac acgtttcctt
       61 atgctcttta aaattcgggg gggagtgctg cacgtgtaat cgataaccaa atttaaaaat
      121 gcacgatcaa aaatttattt agtttttcaa acgacatttt ttttgagagt atagacggca
      181 aagaagtgat tttatttcta gaaaaattgt gagactggag aaagaaaaaa tggaaaaatt
      241 tccttacgaa tattttcaaa acttagaaaa attagagcaa gcaattcaag caatcaagtt
      301 cgatttaaca gacgaaaaat tgttggaata taaaaatatg acacccgaaa gaaaacgatc
      361 gttggaaaaa ttaatgcaac tagtgaacga agacggcgga atttcagacg cagcttttga
      421 cgcatggagc gaatttcatg gagaatataa ttaagtgggt ttaaatggct ctaaaatcgc
      481 ttctaagagt tttttagctt ggaccaatga aatacaccaa aaactaaata aaacgtctta
      541 aatcgaaaaa tacgagctct cagagcgact ttatttgtaa atgttgcata taagaaataa
      601 ttacggattt tagacttttg taggatattt ttgaaataaa tatttatttc tgttataatt
      661 tgattgcaaa taagtgaaca cgaaacacac gaaagcccca gagaggtcgg attctctggg
      721 gctttttttg tgggctgagt cgccttaatt aagttttact tcttgtttcg gttattcaac
      781 cagtaactga acaaagcgct taaaagccca acaagaaggg agcaaataaa gttagtgaac
      841 acgaaacaca cctccttcca gtcgcaagac tgtcggatag gcgacaatca attatacaat
      901 aaaaaagcaa aaataagcct aagtttttta ttgccttggg gttgttaggg cttgccctga
      961 ccgcctgtaa ggcgctttag aacgaaggac atagttttta tcgaatcttg tcttagaacg
     1021 gcttagacga gctctcagga cgccacaaag gagtccctaa tgaattattt ttggcgaagg
     1081 agcctaaagg cgactgctgc aagtaaaatg tgagagcgta gcgatcactc cttcttacag
     1141 cgaccatagg gagctagatt ggcagtttaa tatcatgcgc gaacctgttt attttaaaaa
     1201 gtgggtgatt ttttggcggg aaattttgct cgcgcgcgcg cgtttatttt atttattttt
     1261 taaagatttt ttagaaccct ttttaggggg cagttgagcc ttactctccc aacggataca
     1321 gaagggtact aacaactttt tatctgagta ctaacaactt tttatctgag tactaacaac
     1381 tttttatctg agtactaaca actttttatc tgagtactaa caacttttta tctgagtact
     1441 aatatccttt tgtataataa caaccaacct atttacgagt tggttgttaa aggttattat
     1501 ttatttgtaa ggggggaaga aaggaatgga tgaaaaaacg gaattaacag tcaaatatca
     1561 aaatgaattg aatttagtac cgctgaaaaa tttcaatgct aagcagatgg atttattttt
     1621 tgcattatgc gctcgtatga aagatcgtgg tttgaaaaaa gttagatttt ctttcgagga
     1681 attaaaagag ttaagtgatt ataagatgac tgctacaaaa gcctttgtcg gtgatttaga
     1741 aaagctctat aaagatatgc ttaatcttac ttaccgtact gaagatgagg attctattcg
     1801 ctactttgtt ttgtttactg gttttgaaat caataaaaca aaaaagtttg ttgaagtcag
     1861 tgttaatccg gacctagaat atattattaa tggtttaaca gctgaattta gtagatttga
     1921 attatctgct ttcacgtcgt tgcgttctac gtatgcaaaa actctattcc gactgcttat
     1981 gcagtaccgt tctactggtt attatgtagt tcgtattgaa gagtttagga gcttaatgga
     2041 tatcccagaa tactatcaaa tgggtaacat tgatcaaaaa gtattaaatc ctgcattacg
     2101 agaactaaaa aaatacttca atgatcttaa agtcacaaaa ataaaagcaa aaaagggcaa
     2161 taaaatcgcc aaattagaat ttactttttc aagcctaacg tcagatttgc caaagatttc
     2221 tctgcgtaac tggttagaag aataaataaa aaagaactcg ccccccttac attaagcaag
     2281 tagacaagtc ccttacaacc gactaagatt gctagtacat tctagcatat tgtatggggc
     2341 ttctaaataa aaaacagata ggaataactc ctatctgttt tttatcgttt tagaaaattt
     2401 gttgggtcga aatttctaga agtgactgtt tgactggttt ctttttgatg attctttggt
     2461 cgatttcgat ttgatggatt tagtttagct tcgatttttt ctttcacttc ttgatatact
     2521 ttagaatttg tgccaataag aacgcctgta tcttttagat ataaatcagc aaattctgcg
     2581 atgatttcag tcatgatgga aatttctttt cccatagcat ttagttttgc ctgattgctg
     2641 gtagtggtcg tgttggtatc gggttcataa ctcatacata aaaagcgaat ggcttccgag
     2701 tagttattga tatctggatt tttttcgatg atggaatcta aaacgtcaac atgactaggg
     2761 tacaaagtaa catgtttttg gacagaagtt tttctcatgg ttatatcatt cctccgataa
     2821 gcatttcaat gatttgatta tttgtgtaga catactcgct taatatttga gtattttctg
     2881 tttggatagc aataaattta tcgagcaata aatcgaattg gtcttttttg taagtcgtaa
     2941 gttgacggtc aaaatcactt tcgattaaaa cttttagata tttatttcga ctccattttt
     3001 ctcctgtttt attcgtgagt tcttggcaac gtttgtctat tgttgcaaca aactttggat
     3061 caatattccg aactaaaatg tccataaaaa atctcctttt gcaattatgt ataatttatg
     3121 tataccatac aaatactata tttgtatagc tgttttttgc caaaatgcta tacataagct
     3181 acccacgatg gcgaaaaagt gatatcaaat ttttttctga g
//
