LOCUS       NZ_LR135391             4375 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7933 plasmid 8.
ACCESSION   NZ_LR135391
VERSION     NZ_LR135391.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885473
            Assembly: GCF_900639545.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:50:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,344
            CDSs (total)                      :: 3,251
            Genes (coding)                    :: 3,135
            CDSs (with protein)               :: 3,135
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 39 of 116
            Pseudo Genes (incomplete)         :: 75 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 14 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135391.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7933"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            255..1229
                     /locus_tag="EQB43_RS17420"
     CDS             255..1229
                     /locus_tag="EQB43_RS17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_010727957.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1226..1459
                     /locus_tag="EQB43_RS17425"
     CDS             1226..1459
                     /locus_tag="EQB43_RS17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            1452..1871
                     /locus_tag="EQB43_RS17430"
     CDS             1452..1871
                     /locus_tag="EQB43_RS17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044384217.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGIIM
                     LDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            1880..2188
                     /locus_tag="EQB43_RS17435"
     CDS             1880..2188
                     /locus_tag="EQB43_RS17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333831.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            2192..2554
                     /locus_tag="EQB43_RS17440"
     CDS             2192..2554
                     /locus_tag="EQB43_RS17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            2775..4181
                     /locus_tag="EQB43_RS17445"
     CDS             2775..4181
                     /locus_tag="EQB43_RS17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002333830.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREK
                     ALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     Typing          334..1229
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2776..4178
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.65"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagaagtg ctattattga gttatcaaat aaataaactg aaaaaagagc aaaaaaataa
       61 agtcgagcaa cgctggacac gttctcgacc tttacggaaa ctaatcgcac tagctccgta
      121 acttcttaat cattatacga atatttttag atttcttcaa gactagagat tttttggtaa
      181 tgaattttta agagttacgt gaacttttga aaattctttc cagaagtctc tttttctttg
      241 aggaggaaca aaatttgagt aaaaaaggca cgatcataaa tgagtgggaa aaattaacag
      301 ccccctctaa caggcggtta agtgtccctt ttatggacac ccccctaaaa attcagtggt
      361 caatcgaccg aattaccatt gttggaaatc ttaaagagaa tatctattat cacactacac
      421 atgatgtgtt gattttagat tttgaacagc ttatgcgcct aaatgaaggt aacggttatt
      481 taagatctgt tagtaataat ggttggcagt tgttagacca acatgaagag aatattgctt
      541 atattgaaat tttaaaatgg caagagggga aagggcgtat agacttcaat ccgaataaga
      601 ttagtcaatt cttagctagt tccatgaaaa actttattca tgatttgttt atcgaacctc
      661 acttttcacg tgcagatatt gcttgcgaca ttatcgatgt acctgatgag tttattacac
      721 aatatcgggt agttgatcca gtttctttta agcctattta tggacggaat ggaaaattag
      781 agacggcata ttggggatct cgctcttctg aacgtcaaat tcggatgtat aataaaaaac
      841 tggaacaaga gaagaaaaga aaaattgtgc caaaagaaat cgtgagttgg tggcggttag
      901 aattacaatt aagacgaggg aaagcgactg attggcatgc aatggttcaa gaaagcttgg
      961 atagttttgc tagtcctcat tacttgcctg ctgatacaag tgtagctgac aaaatgatga
     1021 ttactgcttt aacaacagaa catgattatt gggggcaaat taatcgaaag acaaaatata
     1081 aatatcgaaa tttgctgaag caagaaagtc aaaatgatga attgacgaat catttacgag
     1141 aaacctttgc agaatcggca gacgatttaa aaaaagaatt agacacatgg cttctagggc
     1201 tagacgtaac agaggaggaa gaaaaatgac agaaaaacaa aagtattttg gtaagaaaaa
     1261 ttggttggta aatttaacag gtgattacgt tgtggttgtt tttttggata gcaaaaaaat
     1321 gtccggtaag ttacttgatg taacgcaata tgaattgatt ttgaaaaata ataaaattga
     1381 ctctccgatt attatcccta agcacgccgt taaatatatt tatgcggtag ctgaaagtag
     1441 tggagaagct aatgactgat ttgtcagaaa agatgaccat aaaaaatctt gctgacgaat
     1501 tgggtgtatc gaaaaataca gtgcgcaatc aactggtcaa ttcagggttt gaccttgctc
     1561 agtataaaga aaatggcatt atcatgctag ataaagagtt gattggtgtt gttcggtcaa
     1621 tttatcggtc aaaaagtgat caattgactg gtcaattacc ggtcaataac acaggcagtg
     1681 attttccttt agaaaaagag aatgagtttt tgcgtgatca attagctgag aaggacaaac
     1741 atatttctga attaacagaa atgattcgtg atcaacaaaa attgaccctg cttcagcaac
     1801 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
     1861 tctggaagtg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
     1921 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
     1981 tgaaatgctt gaacgaatta tggatgttct caactcggtt atcaatgctg atttagcaaa
     2041 atcttcagtt ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc
     2101 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
     2161 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
     2221 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat
     2281 tgagaaagaa aatgctgcaa tgggcaaggc tcttcggaag cgttttgggt ttgaaacttt
     2341 tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt
     2401 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
     2461 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
     2521 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
     2581 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
     2641 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
     2701 tgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagccga
     2761 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     2821 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     2881 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     2941 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     3001 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     3061 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     3121 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     3181 ttgaatattt caaagaattt aaagaacgga atcctaattt atccgtctac aatgctgtta
     3241 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     3301 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     3361 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     3421 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     3481 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     3541 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     3601 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     3661 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat attgttcctt
     3721 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     3781 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     3841 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctacattgg
     3901 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     3961 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     4021 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     4081 ctgaggaatt agaagtgtta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     4141 agcgtaaatt agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg
     4201 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact aatccctgaa
     4261 gctgacattt gctaaaaaat ggcaagtcca ttttttggcc aacctagcgt tagcgaagtt
     4321 tgggggcgcc cttttcaaag ggtgcggttt tattttttgg atatttgcat agcta
//
