LOCUS       NZ_LR135388             9316 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7933 plasmid 5.
ACCESSION   NZ_LR135388
VERSION     NZ_LR135388.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885473
            Assembly: GCF_900639545.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:50:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,344
            CDSs (total)                      :: 3,251
            Genes (coding)                    :: 3,135
            CDSs (with protein)               :: 3,135
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 39 of 116
            Pseudo Genes (incomplete)         :: 75 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 14 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135388.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7933"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            245..382
                     /locus_tag="EQB43_RS17680"
     CDS             245..382
                     /locus_tag="EQB43_RS17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            824..1204
                     /gene="mobC"
                     /locus_tag="EQB43_RS17255"
     CDS             824..1204
                     /gene="mobC"
                     /locus_tag="EQB43_RS17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            1186..2099
                     /locus_tag="EQB43_RS17260"
                     /pseudo=""
     CDS             1186..2099
                     /locus_tag="EQB43_RS17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2059..2337
                     /locus_tag="EQB43_RS17940"
     CDS             2059..2337
                     /locus_tag="EQB43_RS17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060810720.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVMTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            2339..3028
                     /locus_tag="EQB43_RS17270"
     CDS             2339..3028
                     /locus_tag="EQB43_RS17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127821125.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLIG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKIEEFTACY
                     EDMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3149..3235)
                     /locus_tag="EQB43_RS17275"
     CDS             complement(3149..3235)
                     /locus_tag="EQB43_RS17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3880..4617
                     /locus_tag="EQB43_RS17280"
     CDS             3880..4617
                     /locus_tag="EQB43_RS17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            4645..5184
                     /locus_tag="EQB43_RS17285"
     CDS             4645..5184
                     /locus_tag="EQB43_RS17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            5200..5370
                     /locus_tag="EQB43_RS17685"
     CDS             5200..5370
                     /locus_tag="EQB43_RS17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5682..6143)
                     /locus_tag="EQB43_RS17290"
     CDS             complement(5682..6143)
                     /locus_tag="EQB43_RS17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            6276..6650
                     /locus_tag="EQB43_RS17295"
     CDS             6276..6650
                     /locus_tag="EQB43_RS17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            6704..8830
                     /locus_tag="EQB43_RS17300"
     CDS             6704..8830
                     /locus_tag="EQB43_RS17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            8827..>9316
                     /locus_tag="EQB43_RS17305"
     CDS             8827..>9316
                     /locus_tag="EQB43_RS17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_127821126.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     "
                     /name="ATP-binding cassette domain-containing protein"
     BGC             1276..9316
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3881..4617
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1423..2096
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.556"
                     /coverage="100.0"
                     /evalue="3.29e-174"
ORIGIN
        1 tagataaaga aaactcaact tttataatga actttctgaa aaatttaaca gaaaaagaaa
       61 aaaaagccgt tgtactagta acacatgact taaatgttct aaactatgga actaatatta
      121 tagaattata atttttaccc gtgatgctag ctgatttaaa actagcacca cgggtattta
      181 ttttatattc catctctcct atcccaaaat ttaaataatc acttaataaa aaggaggcac
      241 ccaagtgatt ttattgaaaa aagagtatga aaaattttat gatagactta tttattcttt
      301 gcaattgcca aaaaaattaa accgtcaatt acaaaagact tatgaaacta aatcaactga
      361 agaatgggag agttattgct agatttttgg tagaagtaac aaaaagtgga catattatca
      421 taatgactcg ccgaaagtct gataaaaaat tggctaaaaa tattgctgaa tgtttaataa
      481 aactatcagt tttagctaaa tagttagtag tttacacaaa aaagcattta tgttttttgt
      541 tgcaaagtaa caatttcatc ttcggtgaaa taagcgaaac tttacaaaaa gtggctagca
      601 tttgctagct ttttttgtat tgtggagcgt ggcttttgag ggggattcaa gggggagctt
      661 gcgccccttt gacaagcggt tatttgcact aaatacgtag gtatttggct agcaaatata
      721 gagcttgcca aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat
      781 tgcgaacgat acgtccgtag gacgtactga aagggtggga cttatgagcg aacaaaatca
      841 aaatttggct agcaattctt ctaaaaaata tacctatcga tccgagccaa aacaaatcag
      901 ttttcgagtg agcgaatccg aatttgcaaa gctgaagcaa tcggctgaag ctttgcaaat
      961 gagtgtgccg gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt
     1021 ggcgccagac attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa
     1081 tcaaattgct aagtggtgca atacacacca acatgacgtt gcttctggtg acgctcaacg
     1141 tttatccgaa aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacaattaag
     1201 gtaagccgaa ccacttcttg tagtcgtgca attaattacg cagaaccacg agcaaccatt
     1261 aaaacaggaa taaactgtga cgtcgattat gccaaaagtg aaatgaaaca aattcgcatg
     1321 ctgtatggaa aagacgaccg tgtacaggca cacttattga ttcaatcttt tcgaccagga
     1381 gaaattaccg ctgaaaaagc taatcaacta ggggcagagt atgcaaaaaa atagcccccg
     1441 atcaccaaat agctatttac acacatacag acaaagacca tattcataat cacattgtca
     1501 ttaactccgt taatctggag actggaaaaa aatttcaagc tcatggtcaa gcatttctaa
     1561 acaaatgtta tgacctaaat gacgagattt gtcttgcaca taacttacca atcactgagc
     1621 gaggaaagaa accagaaaaa cgaaccatgt ctgaaataaa attaaaagaa aaaaatgaac
     1681 ctgtttggaa agacgaaatt cggattgcga tcgatcaaac aatgaaaaac aaaaaaacca
     1741 gaacctatga ccaattttgt aattccctca aaacattggg gattcattgc ttcaatcgcg
     1801 ggaaaaattt cacttacgaa ttgatccaca aaaagaaaaa agtacgatcc aacagactag
     1861 gaaaggacta cgaaaaggag acgattttac atgagctgga cagacgagaa cgaacctata
     1921 accaacagcg aaccaacgag ctacgaagag ctcgagctag cattcgcgaa actgcagaag
     1981 aagtacgaag agaacaacga actcttacgc ctgaacaacc ggaaattaga ccggctcgaa
     2041 gaaaccatga acaacacgtt gaaagaggaa cagaccacca acaagaatta tcgctctaaa
     2101 acgttaaaag acatagaaaa cgaaataaag aaacaacgtg cttttcttgc tccctacaca
     2161 caagaaagga tacaaactac gattgattta caagttaaaa aactccatca agagctatta
     2221 gatcagcgaa ataaagaaca aaaaaaggcg ttcattcaac gatcaatgga ttttcttgcc
     2281 cccttatgtt gcttagtcat gacactgttc gcattttggt tagtatgggg aggctaaaat
     2341 ggcattttat ttaccctatc tactcatttt tgttagtatt tcaggaagta tttggctcat
     2401 ctataaaatt tttcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt
     2461 tttcttgatt ggctgtatat tttccttgat aatcgtttca agcggcttat tcggcgtttt
     2521 agagggaaat aagcgcgttt ctagaagtat tttattagga aatgtcactc aaaagtatga
     2581 atcagcacgt gataaaaaga aaaaagagca agcattagca caaaaaattg aagaatttac
     2641 cgcttgttat gaagacatga acgatatttt tgtgaatcaa gaaaaacgtt tgacggataa
     2701 aaacatggaa aaactcactc gtctttatca aaacttgcca gaaaaacagc aaaaagaggt
     2761 tcaagacaac tatgagcgaa ccaaaaaaga cgtgcaatat gtaaaagata caaagataga
     2821 agaagcctgt agtgatctat ttggagacac aaacccttgg tttgccagtg aagaagaaaa
     2881 aaaagaaaaa caacaatctg tcacatatga acgctatgaa atcttacttc aacaagcaac
     2941 taacatgcaa aatccgacaa aaaaagaaac cgccttaaac tatctaaaaa gtgtcaagga
     3001 atggttagat cagcaacaac aaaattgaat ttgatttaaa atcatgttaa actgaatgtg
     3061 caaatgatca aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt
     3121 tttcaggttg atgtcgcctt tattgatttt acttcttgtt tcggttattc aaccaataac
     3181 tgaataaaga gacacaaact cctacaagaa gggggcaaat gaacagttca aacattagat
     3241 acacctcctt ccagtcgcaa gactgtcgga taggcgacaa gttattatat cttaaaaaat
     3301 taaatttgtg tggtgttttt aagtatcact tgttttaggg ttgttagggc ttgccctgac
     3361 cgcctgtaag gcgcttctcg actagattaa gggaaattaa tcaagaattg tcttagaacg
     3421 gcttagacaa gctttcagag tctcacacct cctctttgaa caacggtttt agcaaaggag
     3481 ccggaggcga ctgcgcagta taatatgaga gcatagcgat cacacctttt tagcgaccgt
     3541 agggagcaag ttttccagtt tagaaaaatt cgcgaacctt tttggaggaa aaagtaggag
     3601 ttttttaggc gggaaattca atgtgctctt aagatatact gttagatata ctgttagata
     3661 tactgttaat aataactgtt agggattagc aaccttatgg gagagtaagt attgacatag
     3721 gtagtggggg ataaattgtc acactatggg ggataaattg tcacactatg ggggataaat
     3781 tgtcacacta tgggggataa attgtcacac tatgggggat aaattgtgtt ttacatcctc
     3841 ttatgtctat gataacataa tttattgagg tgatttttat tgagtaacga tatagttcga
     3901 tataataatg gattaaattc tgtcccttta agaaaattta cccctataga tatggattta
     3961 ttttggggaa tatgttctaa aatgaaaaga aaaggtacaa aagaagaaat ttttacattt
     4021 aaacaaataa aagaattaat tgattacaat ataaatgcac gtggcgaccg ttttgctaat
     4081 gatttaataa aaatgtcaaa aaaaatcatt actttgtctt ttatttatga agacgaaaaa
     4141 acatatgaac aattaaatct gtttcaaaaa tttaaaatcg ataaagtgaa tgaaacatta
     4201 ttaataaagg tttctgaaga atttgaatat atacttaatt caatcggaac taattttact
     4261 cgtttcgaat tagaaaatat gacaaactta aagagtagct acgttaaaga gttatatcgc
     4321 caacttatgg ctcataaaga cagaagatct aaaaaaggtc actggtttgt aaaaatagaa
     4381 gattttagag ctctattagc aataccagaa agttatcgca tgagagatat tgataaacaa
     4441 atttttgccc aagcgaaaaa agaattttta aacgttaatt caactcaccc tgctattttt
     4501 aaaacttttg aaatcaaaaa aattaaagcc tacaaaggaa ataagatagg atctctgcaa
     4561 atttattttg aagaaaatat accttcaatt accttagata attggttaga gaaataatac
     4621 ctaacaattt aggaggcaat tatcatgcaa cccaaagaaa cctttacaat aaaggaatta
     4681 tctgaattat ttgaaataag taggcaagca atctctaaac atgttgataa attagacccg
     4741 tccttattag ctaaaaatga gcgtggttat aaggttgtat tacgttctgg agtattgcaa
     4801 cttgctagca acttaggtaa ccaaaggtta ctatataggt tgcatgagta tgaacaagaa
     4861 aacgataaaa acgtttatat atcaagcaaa aacgtgattg taaacgagtt gctagataag
     4921 ttgcgtgaag aaaatgaatt tttacgagaa caactttccc tcaaagagca aaataattct
     4981 aatatgcaaa aattactcga ccagcaacaa cagctcaccc tacaggcaaa tcaacagatc
     5041 gaaaaactgc aagaacagtt acaattagct tatacggaag aaactccaga agatgaatca
     5101 actactattc tttctgaaga aacagaatcg atagaaaaac acacaaaaaa gaagaagcaa
     5161 aaatggtggc aactttggaa ataactggag ggaacattca tgacaagaaa aaatcttcta
     5221 ttcggttcta ttttactagg aattctaggc ttcagtccga tgttattgtc agtaatcaat
     5281 aaagggaata aacatggtga attgaaattg aatccaaatt attacaacag tcctaacgaa
     5341 gaattgtttg taaaagagac ggataaataa taaaaaagac tgctcttccg agcagccagt
     5401 taaaaaataa tgagtgttta ttcttgaaaa agcacgcgct tatgaagcgc tgccaaaaat
     5461 ttaccatatt ttacaaaaaa taacaagtct taaaaaaatg acagataaaa atgcacaaag
     5521 aattagatat agataaccgc gaaaaatcaa gctttagaaa gttgctttta gctagaccaa
     5581 ttaaaaatca caaagtgagc agctatagcc cttgatagag gggctacttg gcatagtcga
     5641 cattatgtca actatgcaat taaataacta actacttcat attagcttaa taaaataatc
     5701 aaaataacta gggggaaaaa cattgtataa aaaaattttt cattttttaa ataagaatag
     5761 taaatataaa aataatttaa gatatctatt actattaatc cagatagagc aaaccaagta
     5821 aatattccat gtgtaaagct aaaaaaattc atttgaatca attcaaataa acctaatata
     5881 gtaacaaata tattaaaaat aatagctgtc aataccagta aattattttt tttatttttt
     5941 gaattatctt catttaaaat agaatttatg ttagtactat aaaattcaca aatttttttt
     6001 agtgtctcta tatctggtgt tgttttgtta ttttcccact ttgaaatagt ttgacgtgta
     6061 acgttaaatt ttcttgccac atattcttga gtataccctt ttttcactct taattctctt
     6121 agcttagatc ctaaatcctc cattttttct cctatgtaaa cgattattta cataaaattt
     6181 tatcataaaa atactaacgc gccaaaaaag ttctttattt tataaaacac actaaaataa
     6241 aataattcta gtaaaaaaga taggagaaat aagttatgaa aaaattttta tgtctttctg
     6301 ctttaatggg tgtcttatta actagtggag gaatagctag tgccacagaa gctttaaatc
     6361 tagatgtacc cgaagaccat accgccattt acggaggagg aatggaagct acagaagaag
     6421 gattttcttc tagtaaattg agatatgctg ctggcggcgg ggatttcaac tgtggggtta
     6481 atggatttaa agtttatgca aattactacc atgctagagc taaacatagt gcaacagcta
     6541 aaaatggacg aggcggtcaa gtacgatcag ttcagaaagc tggagtaaga gcttatgcaa
     6601 catgcaatgc cacacttact ggaaatactg gttggtggaa tgtttattaa aaaagagagt
     6661 ttaaactctc ttttttattt acatttgaaa ggaaattcaa aatatgttaa aaaaaataaa
     6721 cgctgtttta tcactttttg tctgcattac aatactattt ttttctacta atgttataaa
     6781 tggtataaag acttctgaag cgctatataa aaataccgct gaaattcata tgcaatataa
     6841 agatgatatg gactttaaca gttatataaa cgctttgtat gaaatatcta cacaagaaaa
     6901 tattaatatt tctcaatata gttttactgc tgttaaccaa ctaactattc ttgctacaaa
     6961 ccctaacgct aacaaacact ggaaattttc taaaaaaaat atattagcaa gaaacgattc
     7021 taaagtccat tatactaata acaaaaattc taagtaccaa ttaaaacttc caaataattt
     7081 tttaaatata aatatttatc ctttttctca tgtggaaaat attggattaa gtgaaatatt
     7141 atatgtacaa ggaaactcaa acaaattaat tcctgtactg aaaaaatatg gaacacctac
     7201 tatttccaat atcaatcaat cagatacttt tcaaattaat actaatattg ttcttgttat
     7261 ctgctatctt agtatattta ttgtaattac tacaattata tttgcttttt caaaaatgaa
     7321 agaaatcact ctaaaaaaaa tgttaggcta taactctttt gatactataa aagattctat
     7381 taaagagtta aacctcttat tttgttcttt gattataact ttcattttat ctattttttt
     7441 gagcatatat tacagtaacg aaaatttttt cctatcattt cttctatgta gtatttttca
     7501 ttttataatt agttttttat ttatcatagt tactctaagt atctatcatt tatctagtat
     7561 aaaaaattat ctaaaaaatt cacgaccact taagttaaat ctttgtatat taaatatctg
     7621 tctattctta agcatgatcc ttctgttaat agcttctaca aaagtaataa atacccataa
     7681 tgaagccgaa aataattctc ttaaatattg ggaaagaact actaatttat acaaaacaaa
     7741 tattactaat caattaaata gaaacaacac cgtagaagaa aataactact tgaaaaaagc
     7801 tagtaaattt gtttataaaa tacagaagaa ttataaaact tttattatcg cgccatacaa
     7861 ttacgcaact atccaagaga ataataaaga attttttatt ggacaaaaag tttatccaaa
     7921 ttttgaagac tatgttagtc aacctgctgg tgcaggaata tgtgttgatt taaactatct
     7981 aaaatattac aatcctattt cttttgaaga atctactgac ttaaatttaa taaactctga
     8041 tccactaact acttacattc tagtacctaa aaaatataaa gaatatgcaa aaatgattga
     8101 aaaaaattat ttagaagaca tacagtttag acttgaggac tctcctcctc aagctcctta
     8161 taaaaatcct aatttgaaaa atttaaaaat aaaactaata tttgttaata ataaccaaaa
     8221 gtattttagt tttaatacct tatatggaaa ggcaaaagat aactatacaa taactgatcc
     8281 catagttaga gttattaatc cagaaataac agaatcatta ttttggggaa atatattgac
     8341 tagtgatggt ggattatttt ttgatcagaa acatacttca aaccaaaact tttttaatca
     8401 aattaatccg tatataaaag aatttaacct agaaaatatc ataaattttt cttattctgt
     8461 ttttcaagaa actggtgaat tattatctca aaaaaaagta aataaattaa atgcatatct
     8521 acaatatctt ttaatattag gattatttat aataattaat tttcaaatga tttttgttag
     8581 ttttactata aattcaaaaa aaatagcttt aaaaaaaata tttggatatt caccaataag
     8641 tcaagtttta gatttatgtt tactcccttt tacaatagag ttatttgcta ctatgtgttt
     8701 tcaattgata ttttcaaaaa acacttactt gtattttata atttttatga tctttttaat
     8761 aaatctaatt acttctatta ttatttataa aaaaaatagt tttaaatatt ttaaggagaa
     8821 tattttatga tattatcagt tgagaatttc accctgtcaa ttaataaaaa aaagatcaaa
     8881 caaaattgga acttttcaat atcagaaaaa gattttatta tagtaacagg aaaaagtggt
     8941 agtggaaaaa ctactctgtt aaataattta agcctaatag ataaacaata taccggtaac
     9001 ttattctatt ttgaaaagaa agtttctaaa catgatatac aacgtctacg aagaaatcat
     9061 attagctatt tatttcaaaa ctatgctctt cttgaaaatc aaacagtaca atacaatttc
     9121 gaattagcaa aaaagtttaa cagtaatatt tccaatgatc atatttataa tttattaaca
     9181 gatttttctt tgccaaaaga tatcttaaaa gaaaaaatct ttctcttaag cggtggagaa
     9241 caacaaagaa tagcattaat tagaaatatg ttaaaaccat ttgatatatt atttgcagat
     9301 gaacctacag gaaatt
//
