LOCUS       NZ_LR135383             1932 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8172 plasmid 12.
ACCESSION   NZ_LR135383
VERSION     NZ_LR135383.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885602
            Assembly: GCF_900639555.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,748
            CDSs (with protein)               :: 2,748
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 31 of 98
            Pseudo Genes (incomplete)         :: 59 of 98
            Pseudo Genes (internal stop)      :: 31 of 98
            Pseudo Genes (multiple problems)  :: 17 of 98
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135383.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8172"
                     /db_xref="taxon:1352"
                     /plasmid="12"
     gene            655..1620
                     /locus_tag="EQB36_RS15405"
     CDS             655..1620
                     /locus_tag="EQB36_RS15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_125276130.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQLRRG
                     KATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYKYRELL
                     KEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          734..1567
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.141"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 caaaaaggga acaaaaaata caagcatttc tcataataat cgtgagttaa ctgatagcca
       61 aaaaattaat agtttgtctg gtagaaaata attagcagat ttacaagtgg gaaataaaaa
      121 gctgaaagaa gaaaattgca tttcaaacta cattttctac aataactcta aaatggcata
      181 ttttgcgttc tgagcggttt tagcgtttat ttcgtttagt tatcggcaca atcggtgaaa
      241 cacgttagag gagcgaaaat ggagcctgta aggcgactgg tagcggaacg gtcgtgttag
      301 attgtgtagc cgatagctga acgaaataaa tgctggcagc gccattctat ttcggcagga
      361 ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc tttttatttt ttctagagga
      421 gtgctataat caaagaaaat gagaaaaaag agtaaaaaaa tagtcgagca gtgttgacgc
      481 actctcgacc tttacggaaa ctaatcacac tagctccgta acttcttaat cattatacga
      541 atatttttag atttcttcaa gactagagat tttttggtaa tgaattttta agagttacgt
      601 gaacttttga aaattctttc cagaagtctc tttttctttg aggaggaaca aaatttgagt
      661 aaaaatggca taatcgtaaa tgagtgggaa aatttaacag ccctatctaa caggcggtta
      721 aatgttcaaa atttgaacac ccccctaaaa ttacagtggt caattgaccg aattactatc
      781 gttggaaaac tgaaggaaaa tatttattat cacactcaaa atgatgtgtt gattttagat
      841 tttgagcaat taatgcgggt gaatgaaggc aatggctatt taaaagcggt tgggaataac
      901 ggctggcaat tattagacca atacgaagaa aatattgctt atattgagat tttgaaatgg
      961 caagatggca aagggcggat tgattttaac ccgaataaga ttggtcattt tttagctagt
     1021 tcaatgaaaa atttcattca tgatttattt ctagaaccac atttctcacg tgcggatatt
     1081 gcttgcgata ttatcgatgt gcctgatgag tttattacac aatatcgagt ggttgatcct
     1141 gtttctttca agccaattta tgggcggtct gggaagctgg aaacagctta ttggggcagt
     1201 cgtgccagcg aacgacaaat tcggctatac aataaaaaat tggaacaaga aaccaaacga
     1261 aaagttgtac caaaggaagt tgagacttgg tggcggttag aaatgcaact acggcgtggc
     1321 aaggcaaccg attggcataa tatggttcaa gaaagtttga atagttttgc gagcctgcat
     1381 tttttgccga ttgatacaac acctacagat agaataatgc tcgctggttt aatgtctgat
     1441 caaaatttct ggtctctttt gtcgaggaat tcaaaatata aatatcggga attgctgaaa
     1501 gaagaaagtc aaaatgatga attgacgaat catttgcgag aaacgtttgc agaatcggca
     1561 gacaatttga aacaagaatt agacacgtgg cttttgggtt tggacgtgac agagaagtga
     1621 taccgaataa gaaaagtcag atgagtgaga gtaaatttac gtcaatgttt aatgacgtga
     1681 gtttacgata agcgattatt ttgcgtgaag caaaataaag cgtaaaagga gttgagagcg
     1741 tgacacgctt tcggctcctt ttttcgttgt cacttgtgac agcgaatacc ctcggagagc
     1801 atttggaatt gtgacagcca tgtcacaatt aggaaaatgt tactctttgt caaagagtaa
     1861 cagaaaggtt tacactcttt ttattagcga cagcgcgaag gaggggttta acttggggtt
     1921 tagtatttcg tt
//
