LOCUS       NZ_LR135380             4372 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8172 plasmid 9.
ACCESSION   NZ_LR135380
VERSION     NZ_LR135380.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885602
            Assembly: GCF_900639555.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,748
            CDSs (with protein)               :: 2,748
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 31 of 98
            Pseudo Genes (incomplete)         :: 59 of 98
            Pseudo Genes (internal stop)      :: 31 of 98
            Pseudo Genes (multiple problems)  :: 17 of 98
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8172"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            complement(196..1602)
                     /locus_tag="EQB36_RS15335"
     CDS             complement(196..1602)
                     /locus_tag="EQB36_RS15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_127817630.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSENEIEW"
                     /name="plasmid recombination protein"
     gene            complement(1824..2186)
                     /locus_tag="EQB36_RS15340"
     CDS             complement(1824..2186)
                     /locus_tag="EQB36_RS15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045135959.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(2190..2498)
                     /locus_tag="EQB36_RS15345"
     CDS             complement(2190..2498)
                     /locus_tag="EQB36_RS15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333831.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(2507..2926)
                     /locus_tag="EQB36_RS15350"
     CDS             complement(2507..2926)
                     /locus_tag="EQB36_RS15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(2919..3152)
                     /locus_tag="EQB36_RS15355"
     CDS             complement(2919..3152)
                     /locus_tag="EQB36_RS15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(3155..4123)
                     /locus_tag="EQB36_RS15360"
     CDS             complement(3155..4123)
                     /locus_tag="EQB36_RS15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002347008.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVGK
                     LNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQE
                     GKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPV
                     SFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRG
                     KATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLL
                     KRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(3163..4032)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.164"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(200..1602)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagctatgc aaataccaaa aaaataaaac cgcacccttt gaaaagggcg cccccaaact
       61 tcgctaacgc taagttggcc aaaaaatgga cttgccattt tttagcaaat gtcagcttca
      121 ggggctagtg gtttagccgt caactcctta tcctcggcac aaaccgcctc cggtaccgtt
      181 gactgcacat cgtggctacc actcaatctc attctcagaa cgctctaatt tatgctgtga
      241 gacgttttga cgataaaata aggttttctc ctgctctaat acttctaatt cctcagaaag
      301 ccccatatcc ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa
      361 tgcgtttttt aatcgctcta aacgctggcg taaagagcga ttttcttctt tcagcttttt
      421 attttccact tgtaaatctg ctaattgttt tctaccagac aaatgacgct cttcttccaa
      481 tgcagctaca tactcattta attctgtatt ttgctcaagc atctgtaaat attgctcact
      541 catgttatca gcatttctct tggcatttgt tgctctcgta gctcgttgat acaaatcaac
      601 aaaatcattc ccagataaat gatacgaacc atcaggaagc ctcttgaaac tttctaaagg
      661 aaccatatct ttttggaaaa tgaccgccat ctcttttttt acattcagca tatttttctt
      721 ctcttcttca atcttcgctc tctcagaagc catcagagcc ttatcacgct cgttttcttc
      781 tctttgccta taaatatacc tcaactcatc agaaacgctc tcacgctctt ttacagccgt
      841 ttggcgcaat tcagaaattt cactcatgat ttctttgtat tcgtgaatat ctttaatttt
      901 gttggtaccg acaatatccc ttttccaacc gtaatcattc attaatcgtt ctatagagtc
      961 aacttcttga tttctaaaat tattgaaaag cactcgacca ttatctttat cagcgtgtat
     1021 cccttgttga accagtgctt tattaaagct cggctgcttt tgaacacccc gtttataacc
     1081 ttcagcaacc ggcacaatat tcaagtgcag gtgcggactt atttcgtcat tatgaataac
     1141 agcattataa acagccaaat tagggtttct ctcttgaaac tccttgtaat actgaaccaa
     1201 aattttattg gcaatcactc gattttgttc agcttgttgt ttcgatatgc cagtagaaga
     1261 accatctgct cgatttgtct ggaaatcatc taaaatgcct atttgcacaa taaattctcg
     1321 ttgaggttcc aactttttat ccttgcgcac tttcgctaaa tagtcctcga tttttctatc
     1381 ggctcttttt tgtttcgcat tatatttttc taccacttcg ccaaacaatt cctcatattt
     1441 atctgctaga ttcgtctgct ctaaataaat attttcatcc gatttcgtga agtcaatgtg
     1501 cttatgccaa tcattggttt tctggcgatc agttaactca cgattattat gagaaatact
     1561 tgtattcttc gttccttttt taaatgaaat actaaacccc aagttaaaac cctccttcgc
     1621 gctgtcgcta ataaaaagat tgtaaacctt tctgttactc tttgtcaaag agtaacattt
     1681 tcctaattgt gacatggatg tcacaattcc aaacgctctc cgagggtttt cgctgtcaca
     1741 agtgacaacg aaaaaaggag ccgaaagcgt atcacgctat caactccttt tatgctttat
     1801 tttgcttcac gcaaaataat cgcttatcgt aaattcacgt cattaaatat tgacgtaaat
     1861 ttactctcac tcatctgact tttcttattc ggtatcactc cgaataagtc gtcagattca
     1921 tcgggataat ctcgttcgct tggcgctgat ttttcatcag caagccactc attatccact
     1981 gttttttcgt gttgctgatc gtcagaaatt gacgataacc accgctcaaa atcagcaaaa
     2041 gtttcaaacc caaaacgctt gcgaagagcc ttgcccattg cagcattttc tttctcaatc
     2101 aagcgttttt tctgttggcg aagtttcgct tgttcttttt ttaatttttc ttcttgcgct
     2161 ttaatatctg ctaaagtcat tcccataaac tacatctcct tttctttgga attaataacg
     2221 gaaatttggt ttaactgcat aacatgactc attaatttca accaaaattc tttttctgat
     2281 tcatcagttg tttgcgctaa atgcttatga acaaattcat tagtcaaaac tgaagatttt
     2341 gctagatcag cattgataac cgagttgaga acatccataa ttcgttcaag catttcacta
     2401 taattatctt catccggcaa attcaaataa tcagctaacc gttcatacat caattcaatt
     2461 ctatcgactg tttcttctct caaatcaatt ttatccatgt cacacctcac ttccagaatt
     2521 gccaccattt tcgatttttt aattcttgat tttctagtaa caactgatcg tttttttgtt
     2581 gctgaagcaa agtcaatttt tgttggtcac gaatcatttc cgttaattca gaaatatgcc
     2641 tatctttttc cgctaattgt tcacgcaaaa attcattttc tttttctaac gggaaatcat
     2701 tgcctgcatc attgaccggt aaatcaccag tcaattgacc accttttgac cgataaattg
     2761 accgcacaac accaatcaat tccttgtcta acatgataac cccattatct ttataccgag
     2821 caaggtcata acctgcattg accagttgat tgcgcactgt atttttagat atacccaact
     2881 catcagcaag gttttttatg gtcatttttt ctggcaaatc agtcattatc ttctccgtta
     2941 ctttcagcta ccccataaac atatttaaca gcatgcttag ggataatgat tggggaatca
     3001 attttattat ttttcaaaat caactcatac tgcgttacat caagtaactt accggacatt
     3061 tttttgctat ccaaaaaaac aacgacaacg ttatcacctg ttaaattcac caaccaattt
     3121 ttcttaccaa aatgcttttg tttttctgcc attactattc ctcctctgtc acatcaagtc
     3181 cctgtaacca agtatccaat tcttttttta aatcatctgc tgattcttca aatgtttctc
     3241 gtaaatgatt cgtcaactca tcattttgac tttctcgctt taataaattt cgatatttat
     3301 atttcgagtt tcttgacaaa atcgcccaat tagaatgatt agctaacaat ccatcaatca
     3361 taactttatc cgtaggtttt atatcagttg gtaaaaaatg tggactcaca aagctatcca
     3421 aactttcttt caccatagca taccattccg tagctttacc acgtcttaac tgcatttcta
     3481 accgccacca agtattaatt tctgaaggca ccacctttct tttcatttct tgctctattt
     3541 ttttattgta caaacgaatt tgccgctcgc ttgcccgact accccaatac accgtttcca
     3601 gttttcctga gcgtccataa attaatttaa atgaaactgg atcaataatt cgatattgcg
     3661 tgataaactc atcaggcaca tcaataagat cgcaggcaac atccgctcgt gaaaaatggg
     3721 gctctaaaaa taaatcatga ataaaatttt tcatggaatc cgctaaaaat cgactgattt
     3781 tattaggatt aaaatcaatc cgcccttttc cttcttgcca tttagaaatt tcaatatagg
     3841 caatattttc gtcatgttgg tctaatagct gccaaccatt atttccaacc gcttttaaat
     3901 agccattgcc ttcatttacc cgcataagct gttcaaaatc taatattaat acatcatgtt
     3961 gagtatgata aaaaatattt tgattcaact ttccaacaat ggtaattcgg tcaatcgacc
     4021 aatttaattt tatagtttgt accaaagttt gaacatttaa ccgcctgtta gacagggctg
     4081 ttaatttttc ccactcattc acgattgttc tttttttact catattcagt tcctcctcaa
     4141 agaaaaaaga gatttttgga aagaatttcc agagttcacg taactctaaa aattcataac
     4201 catcaatctc tagtcttgaa gaaacctaaa aatattcgta taatgaattt gagttacgga
     4261 actagtgtgg ttagtttccg taaaggtcga gaacgttggt agcgttgctc gacttatttt
     4321 tttgctcttt ttccagttca tttgtttgct aactcaataa tagcacttct ct
//
