LOCUS       NZ_LR135379             4466 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8172 plasmid 8.
ACCESSION   NZ_LR135379
VERSION     NZ_LR135379.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885602
            Assembly: GCF_900639555.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,748
            CDSs (with protein)               :: 2,748
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 31 of 98
            Pseudo Genes (incomplete)         :: 59 of 98
            Pseudo Genes (internal stop)      :: 31 of 98
            Pseudo Genes (multiple problems)  :: 17 of 98
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135379.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8172"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(335..508)
                     /locus_tag="EQB36_RS15595"
     CDS             complement(335..508)
                     /locus_tag="EQB36_RS15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1193..1732)
                     /locus_tag="EQB36_RS15320"
     CDS             complement(1193..1732)
                     /locus_tag="EQB36_RS15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(1748..2671)
                     /locus_tag="EQB36_RS15325"
     CDS             complement(1748..2671)
                     /locus_tag="EQB36_RS15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            complement(3961..4395)
                     /gene="bacA"
                     /locus_tag="EQB36_RS15330"
     CDS             complement(3961..4395)
                     /gene="bacA"
                     /locus_tag="EQB36_RS15330"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     Typing          complement(1749..2671)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatattgtc aagagggttt attttataaa acgtgttatt ttaaaggtta gtagacataa
       61 tgtacactag accaagttgc ccctctacca atggatagag acgctcactt tgtgattttt
      121 aattggtcta ggtaaaaagc tctttttaag gcttgatttt ctgtagttgc ttctttccgt
      181 ttctttgtga atttttacca gtcatttttt actggattgt tattttttat aaaatatgat
      241 aaaattttgg tagcgcttta taagcgcgtt gctttttcaa gaataaactc tcattatttt
      301 tttaactggc tgctcgaaag agcagtcttt tttattattt atccgtctct tttacaaaca
      361 attcttcgtt aggactgttg taataatttg aatttaattt aagttctccg ttggaattgt
      421 tttttccaat tacggataat agcatggggc taaaacctaa tattcctaga gaaatagaac
      481 ccagtaccaa cacagttttt tttaacatag gttatacctc cacttttttt aacgctacca
      541 cttgtataca cattttaaat actagccttc agtttatcaa atgtttaaat tgaacggata
      601 taaatcaaaa ttaaattttc aaaagttgtt ctaaaaaatt tttctttttt ggaagttact
      661 ctaacttttt ttgcttgtgg tgcttggtat gttttcttcg ccgaccgttt aggttcttga
      721 ccattttctc ttataagtgg gatttttacc acgttgagag aaaatggtca agaacctggg
      781 aggcggagaa tgcccttaat accaagcacc gcaagccttt cccatacttt attcgatgat
      841 cccacgatga tctcataatg attctacaat gaccctacga tgatcaaaat tctcaaaaaa
      901 aataaacgaa atttcaaacg atttttttga aattttgtta aaatcacatt tgttttttta
      961 tgactttttc aacaaaatcg attagaattt attttgtttt tccaccaaaa attgagtgag
     1021 atttcaacct attttaagca ataagtcacc atatagtttg atagtttaca actataaaca
     1081 ttgattgttt acagttgtaa actatttttt ctttctcata aaaatttgag tgatctcccc
     1141 atcaggtagt acaacttttt cctctactat ccgaactaac ttctagaaaa ttttagagat
     1201 tggttctatt agatttccaa atttgccacc actttttttc aataagtggt tgtgtattgt
     1261 ctgctttttt ttcagaaaag gcagttgatt cggttgttgg agtttcttct gtataagtta
     1321 aatggagctg ttcttgtagg ttctcaattt gttggttcgc ttgtagagtg agttgttgtt
     1381 gctggtcgag taatttttgc atttgtttta tttgattatc tttttcttgt agctgtatat
     1441 cttttcgatt taactgtttt tctaggaaag aaaggagttt taattcggaa acttggttgg
     1501 tgtttttatg gtttttggtt tttgattttt cgccaactag ttttcgattt aagtttgcgt
     1561 tatcgactaa aaacattgat tttatagctt tttgaccggt ttcgttaatc acaaacttgt
     1621 ttccgttttt tgcaaaccaa agttttcgat ttttgtcaga aacttttttg ttaattgctg
     1681 ttttactaac tcctaattct tccgcaagtt ctttgattgt tttatctgcc ataaatactc
     1741 cttaagctta atcgaataag ttttgtaaat ttcctagtaa atcttgtttg agtttgtttg
     1801 aaagttctcg tttttctgcc tgtttttctt cagtttgttg ttgttgttta aaatcagctt
     1861 catgagtgcc taagggaaga tttagtattc gatctttagc gcgccatttt tcttctcgtg
     1921 ttaattctcc attgtgatca atattaaata attttatacg taaatcttct tgtttccctt
     1981 tggaaaaatc gtcagcatgg ttaatttcag ctttccaagt aaaacgataa ccgataacag
     2041 gtttacctcg acctttacca tattttttac gaatagttaa tcctttaaat aagggagtga
     2101 gttcttcttt aattggcctt aagataaatt tgtcaatatt tgacggacta ttccaataac
     2161 ttttaggaat atctagtaaa tcaaaaaatt tttctttgga aaattcagtc cagccttgag
     2221 ttcgatattg ttttaataat cgaaacatag tttttgaata tgtgctatcc aaattagtaa
     2281 attgctctag attaaatctt gtccagttct ttaattgatt aaaaagtcct ttaaattctg
     2341 gattaactga aattataaca agttgtttat ctcgtataat gctatatttt gtgaaagcaa
     2401 caaatttagt taaagtattt ccatcatcat tccaagcatt aatacttaat aatttgtcat
     2461 acgtgctact aagatctttt ataaattgtt ccccacgttc tgtataaccg cttagttttt
     2521 tcaaatcgtg aaatgaaaaa ataatctcat ctacaccttt ttcacgtaca cgtgaagcaa
     2581 ttgaaaaaaa taagttcatt tcacgactat taaattttct cagaggaata tgatttaatc
     2641 tattttcata ttttacaatt tcatttgcca taagcttctc ctacttttat aaagtatgaa
     2701 ataaaagtaa catactttat aaaaatactc aataatgtcg gaaaacctac ttgaaaagcg
     2761 tagaaaacct acttgaaaag cgtagaaaac ctacttgaaa agcgtagaaa acctacttga
     2821 aaaaactctg aattgcttgg gagagtaagg acacagccga cataaaagag atataaaaag
     2881 agatataaaa agagatataa aaagagggga ggctaatttt cccgcctaaa aaatcactcc
     2941 ttttttctct aaacaggttc gcgaatttat caaaactgcc aattccaaac cttgctccct
     3001 acggttcgct aaaaaggtgt gatcgctctg ctctcacatt atactgcgca gtcgcctccg
     3061 gctcctttgc taaaagccgt tcataaggaa ccctttgaga agctctgagc gttcgtttaa
     3121 gacattttaa gacagtccta gtataaatcc cttatcttgt tcttaaaacg ccttacaggc
     3181 ggtcagggca agccctaaca accctaaaat tgtctaaatg ccctctaaaa ttcgatttaa
     3241 gcactcttaa acctattctt atatatttat atacttaggc gtttcaaacg tcttaaaacg
     3301 caaattagag cttgataaag atatttgctc gtgtgcctag acttttaaaa aagtcggcac
     3361 acgccatgac aaagctgaaa taaactgctt cagctttgtc atggcggatt cgctcacttg
     3421 aaaaagtgtt tatttcgctc gattattagc atatattttt tagctttttt gctagccaaa
     3481 ttttgcatta atttgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     3541 gtaatgtgaa agtaccttta aaggtacttt ctacaaaact cactaaaagg gtttggcaag
     3601 ctctatattt gctagccaaa tacctccgta tttggtgcaa ataaccgctt gtcaaagggg
     3661 cgcaagctcc cccttgaatc cccctcaaaa cccacgctcc acaatacaaa aagtggctag
     3721 aaaatgctag ccactttttg taaaatttag tatgatttat ttttagctat ttagagtttt
     3781 tatttccgta aatacttcta aaaattgcta agataaatgc tataggaatt agataaagaa
     3841 tacttttttg taagcttata ttgaaaatta gcctaaatat gaagaacata gtgattaaaa
     3901 ctattattga aataattaca gctaaaatct ttttattcat gagatagttt cactttctga
     3961 ttattttatt acataacgag cgtaatcatt gtattgatat gatgttgtat tgctaaagtg
     4021 agcattatat gttaattgta caccttttcc ttgatttgca gctcttacaa agacattcat
     4081 tgcatcaaca gcactactaa aagcatttgt aataacccat gttccaactc caccaatagg
     4141 aataaaagat gcaatttgag caggagtctt tagcttatta taattttttt tcaaatctct
     4201 acacatttta ttagtaacat aatagttcat tttatagcaa gtaaatccac gatgattatg
     4261 attatatcgt gaacttgcag cttccacttt tatacctcta gcactaaaag gaaacagatt
     4321 taacggagta cttgctccaa caatcatacc gaacaaaaat agacaagtaa taattttaat
     4381 aattttattt ttcattgtta cttcctcctt agatataaaa tcctaggaaa gcgctttctg
     4441 tatatattta gaataatcag aaaatt
//
