LOCUS       NZ_LR135378             5201 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8172 plasmid 7.
ACCESSION   NZ_LR135378
VERSION     NZ_LR135378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885602
            Assembly: GCF_900639555.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,748
            CDSs (with protein)               :: 2,748
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 31 of 98
            Pseudo Genes (incomplete)         :: 59 of 98
            Pseudo Genes (internal stop)      :: 31 of 98
            Pseudo Genes (multiple problems)  :: 17 of 98
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135378.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8172"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            complement(<1..293)
                     /locus_tag="EQB36_RS15290"
     CDS             complement(<1..293)
                     /locus_tag="EQB36_RS15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127817629.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEV"
                     /name="hypothetical protein"
     gene            complement(295..576)
                     /locus_tag="EQB36_RS15295"
     CDS             complement(295..576)
                     /locus_tag="EQB36_RS15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125394241.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIDIQTKNTETLNDYVHTNNQIIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(952..1476)
                     /locus_tag="EQB36_RS15300"
     CDS             complement(952..1476)
                     /locus_tag="EQB36_RS15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_125394239.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVVEKTKTENHKNANQVPQLEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTILSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(1494..2462)
                     /locus_tag="EQB36_RS15305"
     CDS             complement(1494..2462)
                     /locus_tag="EQB36_RS15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002332718.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIAYFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            3612..4631
                     /locus_tag="EQB36_RS15310"
     CDS             3612..4631
                     /locus_tag="EQB36_RS15310"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_010729551.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGHFNS
                     FFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCYDNPK
                     YKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYLY
                     GGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKLKIMIV"
                     /name="Abi family protein"
     Typing          complement(1499..2462)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.661"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acttcttgat atactttcga gtttgtaccg ataagaacgc ctgtatcttt taaatacaaa
       61 tcagcaaatt ctgcaatgat ttcggacata atagaaattt cttttcccat cgcatttagt
      121 tttgcttggt tgctggtagt tgtcgtatta gtattaggtt cataactcat acatagaaaa
      181 cgaatggctt ccgagtagtt attgatatct ggattttttt cgattatgga atctaaaacg
      241 tcaatgtgac tgggataaag ggtaacgtgt ttttgaacag aagtttttct catgttatat
      301 tcctccgata agtgtttcaa tgatttggtt gtttgtatga acatagtcgt tcaatgtttc
      361 agtatttttt gtttgaatat cgataaattt ttcgagtaat aaatcgaatt gatctttttt
      421 gtaattcata agttgacggt caaaatcact ttcgattaga acttttagat atttatttcg
      481 actccatttt tctcctgttt tattcgtgag ttcttggcaa cgtttgtcta ttgttgcaac
      541 aaactttgga tcaatattcc gaactaaaat gtccataaaa aatctccttt tgcaattatg
      601 tataatttat gtatatcata caaatactat atttgtatag ctgttttttg ccgaagcact
      661 atacataagc tatccataat ggcgaaaaag tgatagcaaa tttttttctt agaaacgtgg
      721 taggcacagc ctacctagca cctttcgtaa gaaagtgata ccacgtttcc ttatgctctt
      781 tcaaaattca gaaaaatcag ctgtaaaacg agtgcagtcc gagtaagatt caacgaaaaa
      841 acggggattg gggcgccacc aaaatatcgc aacatgggcc catgttgctc tgctcgcaaa
      901 agcaaaaaga agaataaaac cagcaattta aattaattta aattgctggt tttattgcca
      961 gaattgccac cattttttct ccgtctttga ttgtttttct atcgattctg tttcttcaga
     1021 aagaatcgtt gatttatttt ccgaagattc ttccgtataa gtcaactgta gttgttcctg
     1081 cagtttctcg atttgttggt tggcttgcag ggtgagttgt tgttgttggt cgagtaattt
     1141 ttgcatttgt tttatttgct cgtctttttc ttgtagttgc atatcttttt gatttaactg
     1201 ttgttctagg agagaaagga cttctaattg gggaacttgg ttggcgtttt tgtggttttc
     1261 ggttttcgtt ttttcgacaa ctagttgttg atttgagttt tcattttcgt taaaaaacat
     1321 tgattttata gccttttgtc cggtttcatt aatcacaaac ttgtttccga ttttcgaaaa
     1381 ccaaagtttt cgattttcgt cagaaacttt tttgttaatt gctgttttac taactcctaa
     1441 ttcttcagca agttctttga ttgttttttc tgtcataaaa tctaaaaacc ccctcaatct
     1501 ttgaataagt tttgaagatt gtctaataag tcttttttta gttctgaagg aatatcggaa
     1561 tttatctctg atttttgtgc attattttct ttattttggg tattttgttc tctttctaac
     1621 ttttctaact gttgtaaaag ttccaatttt cgcttttgtt ctttgatttt ttcttgtaat
     1681 gaagtaggtg ctttttcaga ttgaacttgt tggttagcta gttctaatgc tttgttttgt
     1741 tcttcttttg ttggggcatg tttgttaaag tcgtctgatt tttttatctc aggtttaaat
     1801 gtaaattgat agccaatgac aggctttccc cgtccttttc cgtatctctt ttggattgtc
     1861 agacctttga agattgcggt tagttcttgg cgaataggct ttataatttt tttatttata
     1921 tcactttctc tataactttt aggtatatca agtaattcat gaaagtcttc ttttgaaaaa
     1981 taggcaattc ctttggttct gtattgtttt aataatctaa acatggtttt ggaataactg
     2041 ctttctaatt cattgaattg ttggagagaa tatcgaaccc attcttcaag attattaagt
     2101 aagggtaagg ctttctcatg gacttgtatt tcagcaaaag gaatatcagc ttttccatcg
     2161 attttaaatt gattaaataa aacaaatcgt tcacgttgca aaccgtcagc acttcttcta
     2221 ccaaaacgta aatccattaa tttgtcatac gttgtttcta aatcatctat gaatcgaaca
     2281 tttgcagttg ctttataatt gcttaaatct tttaattgtt cgaatgtaaa ttgtactttt
     2341 ttatcgccta cgtcacgcat tctagatact atagagaaga ataaattcat ttctactgac
     2401 gtaaatttgc gtaaaggaat cgtatttaat tcatggtggt atttcacaat ttcatttgcc
     2461 ataaaatcct ccttcaagat taactactta acaaaattat aaagagcttt ctaatagggg
     2521 tcaagcccct aattaaagac ccctaaaaac agacaaaaag acccctaaaa acagacaaaa
     2581 agacccctaa aaacagacaa aaagacccct aaaaacagac aaaaagaccc ccaaaagatt
     2641 ctgcacacct tgggagagta agcatacagc cgacggtaaa gaggtttaaa aagaggttta
     2701 aaaagagatt ataaaagagg agaggggcaa aatttcccgc ctaaaaattt tctgcttttt
     2761 tagccaaaca ggttcgcgaa ttttactaaa ctaggaaact tgctccctct ggtcgctaaa
     2821 aaggtgtgag ggaaaaacac cctcacattg tacctgcgca gtcgcctaaa ggctcctttg
     2881 ctaaaaacgt tgtttaaaga gattttttta gaggctctgc gcgctcgttt aagccgtttt
     2941 aagacagttt agtatggata aacccttatc ttgttcttaa agcaccttac aggcggtcag
     3001 ggcaagccct aacaaccctt aaagagcata aaaccatcct aaaattcgat ttaatctgtc
     3061 ttatctatgt tcttgtatat tttatatacc ttgggtagta aaacgtctga aaacgcaaat
     3121 aagagcgtat gaaacaaaac aataagcaac cgattcagca gaattccgga agagccattt
     3181 tcgtacgtta gcaaaaaagt gctgttgcta aaagctgctt gcggtgcgga gcgaacaagg
     3241 tctcctagag cgagacgtgt acaaacaagc ttacaaattc gtgaaaggca ccggttttgc
     3301 gccattttca agcaaaaaaa ggtctatcga gcttgctcga tagaccgatc gcatttagcc
     3361 ccccgcattc taacaggggg gggataatgt gaaaaattat gatagtaatg cttgatatat
     3421 aggcatttct tttggttgca cacttaaaaa aaatcacgat tttgacaact aaatttttaa
     3481 ataaaaaagc tttacagttt aattcaaatg tgttttaata tatctatcag tacttgccac
     3541 gcctctgatt taatatcatg cgcattatgg tagggctttt tttttatatt tttttaagaa
     3601 agaagattct tatgaagaca tcaaatagaa aatataaagt taaaacagtt aatcatagaa
     3661 ttccacctaa aaaggcattg tgtgtaatta ataattccaa agtaattaat caagagttaa
     3721 aaccatattt atccgagcaa gaaatgataa aaaaaataag taaagatatt tttttaacaa
     3781 attccgatac aattttatta gaaaagtttt taaagaaaaa tagttatttt cgatttagta
     3841 tttatgttaa attaatgaaa aatatagata aagttactat tctagatgtt attgaaacgt
     3901 acaaattaga ttcattcatt cgtaatcaat tgcattattt tgtgaatcaa atagaaattt
     3961 tttggaaaaa atctctttca gataatatgt gcgtttctta tgaagaaact agcatatttc
     4021 cgaagaatca gtgttatttg gataaaaata tttattcaga tttgaaatgg gctgaagata
     4081 taattggtca ttttaatagt tttttttatt caaatcaaag tcccactttt aaacatcatc
     4141 atataaaaaa aaatcattgt ttaccaatat gggctttatt tgaagaaata acatttggta
     4201 gtttaacaac atttattaat caacttaata cagagtatta taataattgg gtcatgagct
     4261 gttatgataa tcctaaatac aaaaaagcta tacgaagttg ggcaaacgta attagagctt
     4321 atagaaataa gatagctcat cattcaagaa tatatgcatt taaagttact gacgctccaa
     4381 caatcataaa aaatgattcc aaaagttatt ttcctgaaaa taacttaaaa gaaacccaaa
     4441 agttatattt atatggagga ttatacgtta taaaacatct gttaatatat gaagatagca
     4501 tgacacaaac tttatggaat aaatttctaa tagatttaga aaaaaagatc gataaaatcc
     4561 cttccttaga aaaatccaat tatggttttc cggaaaattg gagaaacaag ttaaaaataa
     4621 tgattgttta attgttattt aaattgttag ttaacataat gtacactaga ccaagttgcc
     4681 cctctaccaa tggatagaga cgctcacttt gtgatttttc attggtctag ctaaaagaga
     4741 ctttctaaag cttgatttga tacgatttgt ttcttccatt tctttgtgaa tttggaccgg
     4801 tctgttttta gggggttgtt atttttgtga gcgcttgtga gactgctctt tcgagcagtc
     4861 tttttttttg attgtttgcg tataataaaa aagatcgtta cattcgcaaa atttgttaca
     4921 cttattttac ccttaaaaca agctaacttt ttcttgttta gctgctcgta cgagcagctt
     4981 ttttttttac gcaaattggg atcgatttgc aaatcttatt ggaagaccgc tctctagaaa
     5041 ataaaccagg aaatttttaa tttcctggtt tattttttta tctttttaga aagttggttg
     5101 ggtcgaaatt tttagaagtg aatgtttgac tcgtttcttt ttgacgattc tttggttgat
     5161 ttcgttggga gggatttagc tgtttttcaa ttttttcttt t
//
