LOCUS       NZ_LR135377             6095 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8172 plasmid 6.
ACCESSION   NZ_LR135377
VERSION     NZ_LR135377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885602
            Assembly: GCF_900639555.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,748
            CDSs (with protein)               :: 2,748
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 31 of 98
            Pseudo Genes (incomplete)         :: 59 of 98
            Pseudo Genes (internal stop)      :: 31 of 98
            Pseudo Genes (multiple problems)  :: 17 of 98
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8172"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            complement(346..879)
                     /locus_tag="EQB36_RS15255"
     CDS             complement(346..879)
                     /locus_tag="EQB36_RS15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(1340..1510)
                     /locus_tag="EQB36_RS15590"
     CDS             complement(1340..1510)
                     /locus_tag="EQB36_RS15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322958.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1526..2059)
                     /locus_tag="EQB36_RS15260"
     CDS             complement(1526..2059)
                     /locus_tag="EQB36_RS15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_104858994.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNDRGYKVV
                     LRSGVLQLARNLGNQRLLDKLHEHEQNSDKNAYIPTKNVVANELLDQLREENKFLREQI
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIE
                     KQTKKKQKWWKLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(2087..2824)
                     /locus_tag="EQB36_RS15265"
     CDS             complement(2087..2824)
                     /locus_tag="EQB36_RS15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002292475.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKNRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            complement(3675..4364)
                     /locus_tag="EQB36_RS15270"
     CDS             complement(3675..4364)
                     /locus_tag="EQB36_RS15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125276269.1"
                     /translation="MAFYLPYLLIFVSISGSIWLSCKIYQTRHSLKGSKIRFKRFLLLG
                     CIFSLIIISSGLFGILEGNKRISGSILLENVTQKYEVARDKKKKEQALAQKIEEFTACY
                     ENMNDIFVKQEKRLTDKNMETLTRLYRKLPEKQQKEYQEKYEQVKKDVQYVKDTQTEEA
                     CYDLFSDTIPFSTSEQERKERQQSVTYERYEILLQKATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4604..5542)
                     /locus_tag="EQB36_RS15280"
     CDS             complement(4604..5542)
                     /locus_tag="EQB36_RS15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_104858995.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDHVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNPKTRTYDQFCDSLKIFGIHCFNRGKNFTYELIKKKKKVRS
                     NKLGKDYEKETILHELDRREQTYYRERERAYHRQQADELRRARESIRKIAGEVRREQRT
                     FTPEQSEIRSTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5524..5904)
                     /locus_tag="EQB36_RS15285"
     CDS             complement(5524..5904)
                     /locus_tag="EQB36_RS15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2088..2824)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4608..5542)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.705"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccacgctcca caatacaaaa aaaggctagc atttgctagc cactttttgt aaagtttcgc
       61 ttatttcacc gaaggtgaaa ttgttgcttt gtaatattca aacaacccta ttcttctaaa
      121 atgtatcgat ttattttttt ttagacttta ttatgtctaa gacaactaag attaacacta
      181 aaaataaacc agatttgtat aattgccctc cgcttagcat attaacaatt atagcaacag
      241 ctatagcaaa tataaaaata cgaatagtag ctatttgcgg attcttttta gtaaatgttt
      301 ttaatgattt taatgattta gatatcaaaa acatcactcc ttaacttata acgttgaata
      361 tctagtagct acaactcttc cagctgcatc atatcccctt tggtaataaa atccaatcca
      421 gccattacca tttttgtccc attcttttgc aactgcattc cctggagaag cacctttttt
      481 agtccaactt gcacctgtta acaaagcacc aattgcccaa cctgggatac caaacataga
      541 agtgctagct ttccctcctg cccaaaaggc agcattaata gcattagcag ctactccagt
      601 ggttacagca atatttgtat attccttttt gccccatctt gcaactgcag ctcttgtcat
      661 gatatctgaa gaatttgcaa aatcttccgc actataacca tcctcatatt tagttacgac
      721 cataccatct tcagtgtact caataactac attttcagta gcattgtttt cgtttaatga
      781 taccacttgg gaattttctg aagcataaac tatagtagtt gacggtaaaa caactgcccc
      841 aatagttaac aaagctaccc ctaaagtaaa ttttttcata gaatttcctc ctgatacaga
      901 aaaatatata gaaaacgttt tctttgtgaa aatcataaaa caaaaggtat aggcttgtca
      961 atattttatt tagtaaagat aatttatatt tttgataatg tggtatagtt agaatgcatt
     1021 aggaaaatat gttatattaa attcttgttg acataatgta cactatgcca agtagcgcct
     1081 gtagcaatcg atacaggcgc tcactttgtg attttcaatt ggtctagcta gttactagct
     1141 tctaatgctt gatttgatgc aatttctctt ttttagttct ttgtgcattt ttaccggtca
     1201 ctttttaaag acttgttatt ttttagagga tttgatatgc tttttttgtc cttcttctag
     1261 ttcttcattt tgtaatattt ttatgttgat gaataggata atttcatttt gggctgctcg
     1321 gaagagcagt ctttttttat tatttatccg tttcttttac aaataactct tcgttaggac
     1381 tgttgtaata atttggattc aatttcaatt caccatgttt atttccttta ttgatcactg
     1441 acaataacat cggactgaag cctagaattc ctagtaaaat agaaccaaat atgagctttt
     1501 ttcttgtcat gaatgtttcc tccagttatt tccatagttt ccaccatttt tgcttcttct
     1561 ttgtttgttt ttctatcgat tctgtttctt cagaaagagt agttgattca ttttctgaag
     1621 tttcttccgt ataaactaat tgtaaccgtt tttgcagttt atcgatttgt tgatttgcct
     1681 gcaaggtgag ttgttgttgc tggtctaaga gtttttgtat atgagaatta ttttgttctt
     1741 tgagagcgat ttgttcacgt aaaaatttat tttcttcacg caactgatcg agcaactcat
     1801 ttgcaaccac gttttttgtt ggtatataag catttttatc gctgttttgt tcatgttcat
     1861 gcaacttatc tagcaacctt tggttgccta aattgcgagc aagttgcaat actccagaac
     1921 gtaatacaac cttataacca cgatcatttt tagctaataa ggacgggtct aatttatcaa
     1981 catgtttaga gattgcttgc ctacttattt caaataattc agataattcc tttattgtaa
     2041 aggtttcttt gggttgcatg ataattgcct cctaaattgt taggtattat ttctctaacc
     2101 aattatctaa ggtaattgaa ggtatatttt cttcaaaata aatttgcaga gatcctatct
     2161 tatttccttt gtaggcttta atttttttga tttcaaaagt tttaaaaata gcagggtgag
     2221 ttgaattaac gtttaaaaat tcttttttcg cttgggcaaa aatttgttta tcaatatctc
     2281 tcatgcgata actttctggt attgctaata gagctctaaa atcttctatt tttacaaacc
     2341 agtgaccttt tttagatctt ctgtttttat gagccataag ttggcgatat aactctttaa
     2401 cgtagctact ctttaagttt gtcatatttt ctaattcgaa acgagtaaaa ttagttccga
     2461 ttgaattaag tatatattca aattcttcag aaacctttat taataatgtt tcattcactt
     2521 tatcgatttt aaatttttga aacagattta attgctcata tgttttttcg tcttcataaa
     2581 taaaagacaa agtaatgatt ttttttgaca tttttattaa atcattagca aaacggtcgc
     2641 cacgtgcatt tatattgtaa tcaattaatt cttttatttg tttaaatgta aaaatttctt
     2701 cttttgtacc ttttcttttc attttagaac atattcccca aaataaatcc atatctatag
     2761 gagtaaattt tcttaaaggg acagaattta atccattatt atatcgaact atatcgttac
     2821 tcaataaaaa tcacctcaat aaattatgtt atcatagaca taagaggatg taaaatacaa
     2881 tttatccccc atagtgtgac aatttatccc ccatagtgtg acaatttatc ccccatagtg
     2941 tgacaattta tcccccatag tgtgacaatt tatcccccac tacctatgtc aatacttact
     3001 ctcccatgag gttgctaata cctaacagtt attattaaca gtatatctaa cagtatatct
     3061 aacagtatat cttaagagca catttaattt cccgcctaaa aaactactac cttttactca
     3121 aacaggttcg cgaatttttc taaactggaa aacttgctcc ctacggtcgc taaaaaggtg
     3181 tgatcgctat gctctcacat tgtactgcgc agtcgcctaa aggctccttt gccaaaaccg
     3241 ttgttcaaag agaaccttgt ggggctctga gccctcgtct aaaccgtttt gagacagttc
     3301 taggggaaaa ccttatcagt tcctaaagcg ccttacaggc ggtcagggca agccctaaca
     3361 accctaaaat aagggatact taaaaacacc acacagattt aattttttaa gatataataa
     3421 cttgtcgcct atccgacagt cttgcgactg gaaggaggtg tatcttatga ttacccactt
     3481 tatttgcgcc cttcttgttg agcttctaaa ctctttattt agttattggc tgaataaccg
     3541 aaacaagaag taaaatcaac taaggcgata tcagcctaca aaaaaagtcc tagagaatcc
     3601 gacttctcta ggactttcgt gtatcttatg attacctatt tgcatattta gtttaacatg
     3661 agtttcaatc aaattcaatt ttgctgttgc tgatccagcc attctttgac actttctaga
     3721 taatttaatg ctgtttcttt tttcgttgga ttttgtatgt tagttgcctt ttgaagcaag
     3781 atttcatagc gttcatatgt gactgattgt tgtcgttctt ttcgttcttg ttcgctagtt
     3841 gaaaaaggga ttgtatcact gaataagtca taacacgctt cttctgtttg cgtatctttc
     3901 acatattgca cgtctttttt tacttgttca tatttttctt ggtactcttt ttgctgtttt
     3961 tctggcaatt tccgataaag acgagtgagt gtttccatat ttttatccgt caatcgcttt
     4021 tcttgcttca caaaaatatc attcatgttt tcataacaag cggtaaattc ttcaattttt
     4081 tgtgctaatg cttgctcttt tttcttttta tcacgtgcta cttcatactt ttgagtgaca
     4141 ttttctaata aaatacttcc tgaaatgcgc ttatttccct ctaaaatgcc gaataagcca
     4201 cttgaaatga ttatcaatga aaatatacag ccaagcaata aaaatcgctt aaatcgaatt
     4261 ttagagcctt tcaatgaatg tcttgtttga taaattttac aactgagcca aatacttcct
     4321 gaaatactaa caaaaatgag tagatagggt aaataaaatg ccattttagc ctccccatac
     4381 taaccaaaat gcgaacagtg tcaggactaa gcaacataag ggggcaagaa tatccatcga
     4441 tcgttgaatt aaagcctttt tttgttcttg gtttcgctgg tctaatagcg cttggtggag
     4501 ttttttgact tgtgaatcaa ttgtagtttg tatcctttct tgggtataag gcgcaagaaa
     4561 agcacgttgt ctttttattt cgttttctat gtcttttaac gttttagagc gataattctt
     4621 gttggtggtc tgttcctcgt tcaacgtgtt gttcatggtt tcttcgagtc gatctaattt
     4681 ccgattgttc aggcgtaaaa gttcgttgtt ctcttcgtac ttctccagca attttccgaa
     4741 tgctttctcg agctcttcga agctcgtcgg cttgctgtct atgataggct cgttctcgtt
     4801 ctcgataata ggtttgttct cgtctgtcca gctcatgtaa aattgtctcc ttttcatagt
     4861 cctttcctaa tttgttggat cgtacttttt tctttttttt gattaattcg taagtaaaat
     4921 ttttcccacg attgaagcaa tgaatcccaa atattttgag ggaatcacaa aattggtcat
     4981 aggttctggt ttttggattt ttcatggttt gatcaatggc aaagcgaatt tcgtctttcc
     5041 aaacaggttc atttttttct tttagtttga tttcagacat agttcgtttt tcttcttttt
     5101 ttcctcgttc agtgatactt aaaccatgag tcaaacaaat ttcgtcgttg atatcgtaac
     5161 acttatctag gaatgcttga ccatgtgctt gaaacttttt tcctgtttct aaattgaccg
     5221 aattaatgac aatgtgattg tggatatggt ctttgtccgt gtgcgtataa atcgcgattt
     5281 ggtgttctgg tgcgattttt tcagcgtatt cttttcctag ctggttggct ttttcggcgg
     5341 taatttctcc tggtcgaaag gactgaatca ataagtgtgc ttgtacatga tcgtcttttc
     5401 cgtaaagcat acgtatttgt ttcatttcac ttttggcata atcaatgtca cagtttattc
     5461 ctgttttgac ggttgctcgt ggttctgcgt aattgattgc tcggctacat gaagtggttc
     5521 gacttacttt aactgttgcc atatcttttg tagctccttt tgcatgattt ttaggttttc
     5581 ggataaacgt tgagcatcat caggagcaac gtcgtgttgg tgtgtattgc accacttggc
     5641 aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct gggcaatgtc
     5701 tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca cgaaagccgg
     5761 cacactcatt tgcaaagctt cagctgactg cttcagcttt gcaaattcgg attcgctcac
     5821 tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaat gatcgctagc
     5881 caaattttga tgttgttcgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     5941 gttcgcaata ccattgtacc taaaaaggta caatgagtaa agctcactaa aagggtttgg
     6001 caagctctat atttgctagc caaatacctc cgtatttggt gcaaataacc gcttgtcaaa
     6061 ggagcgcaag ctcccccttg aatccccctc aaaat
//
