LOCUS       NZ_LR135376             6303 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8172 plasmid 5.
ACCESSION   NZ_LR135376
VERSION     NZ_LR135376.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885602
            Assembly: GCF_900639555.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,937
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,748
            CDSs (with protein)               :: 2,748
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 31 of 98
            Pseudo Genes (incomplete)         :: 59 of 98
            Pseudo Genes (internal stop)      :: 31 of 98
            Pseudo Genes (multiple problems)  :: 17 of 98
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135376.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8172"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(382..915)
                     /locus_tag="EQB36_RS15210"
     CDS             complement(382..915)
                     /locus_tag="EQB36_RS15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(1365..1535)
                     /locus_tag="EQB36_RS15585"
     CDS             complement(1365..1535)
                     /locus_tag="EQB36_RS15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1551..2084)
                     /locus_tag="EQB36_RS15215"
     CDS             complement(1551..2084)
                     /locus_tag="EQB36_RS15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(2105..3055)
                     /locus_tag="EQB36_RS15220"
     CDS             complement(2105..3055)
                     /locus_tag="EQB36_RS15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            3731..3817
                     /locus_tag="EQB36_RS15225"
     CDS             3731..3817
                     /locus_tag="EQB36_RS15225"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3938..4627)
                     /locus_tag="EQB36_RS15230"
     CDS             complement(3938..4627)
                     /locus_tag="EQB36_RS15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4872..5786)
                     /locus_tag="EQB36_RS15240"
     CDS             complement(4872..5786)
                     /locus_tag="EQB36_RS15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5768..6148)
                     /locus_tag="EQB36_RS15245"
     CDS             complement(5768..6148)
                     /locus_tag="EQB36_RS15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2106..3054)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4876..5786)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggagcgcaa gctccccctt gaatccccct caaaatccac gctccacaat acaaaaaaag
       61 gctagcattt gctagccact ttttgtaaag tttcgcttat ttcaccgaag gtgaaattgt
      121 tgctttgtaa tattcaaaca accctattct tctaaaatgt atcgatttat ttttttttag
      181 actttattat gtctaagaca actaagatta acactaaaaa taaaccagat ttgtataatt
      241 gccctccgct tagcatatta acaattatag caacagctat agcaaatata aaaatacgaa
      301 tagtagctat ttgcggattc tttttagtaa atgtttttaa tgattttaat gatttagata
      361 tcaaaaacat cactccttaa cttataacgt tgaatatcta gtagctacaa ctcttccagc
      421 tgcatcatat cccctttggt aataaaatcc aatccagcca ttaccatttt tgtcccattc
      481 ttttgcaact gcattccctg gagaagcacc ttttttagtc caacttgcac ctgttaacaa
      541 agcaccaatt gcccaacctg ggataccaaa catagaagtg ctagctttcc ctcctgccca
      601 aaaggcagca ttaatagcat tagcagctac tccagtggtt acagcaatat ttgtatattc
      661 ctttttgccc catcttgcaa ctgcagctct tgtcatgata tctgaagaat ttgcaaaatc
      721 ttccgcacta taaccatcct catatttagt tacgaccata ccatcttcag tgtactcaat
      781 aactacattt tcagtagcat tgttttcgtt taatgatacc acttgggaat tttctgaagc
      841 ataaactata gtagttgacg gtaaaacaac tgccccaata gttaacaaag ctacccctaa
      901 agtaaatttt ttcatagaat ttcctcctga tacagaaaaa tatatagaaa acgttttctt
      961 tgtgaaaatc ataaaacaaa aggtataggc ttgtcaatat tttatttagt aaagataatt
     1021 tatatttttg ataatgtggt atagttagaa tgcattagga aaatatgtta tattaaattc
     1081 ttgttaacat aatgtacact atgccaagta gcccttgtat caaccgatac agaggctcac
     1141 tttgtgattt tcgattggtc taggtcaaaa gctcttttta agcttgattt tctgcggttg
     1201 cttctttccg tttctttgtg aatttttacc agtcattttt tactgggttg ttatttttaa
     1261 taaaatatga taaatttttg gtagcgcttc ataagcgcgt tgctttttca agaataaaca
     1321 ctcattattt tttaactggc tgctcgaaag agcagtcttt tttattattt atctgtttct
     1381 tttacaaaca attcttcgtt aggactgttg taataatttg gattcaattt caattcacca
     1441 tgtttattcc ctttattgat cactgacaat aacatcggac tgaatcctag aattcctagt
     1501 aaaatagaac cgaatagaag attttttctt gtcatgaatg ttccctccag ttatttccaa
     1561 agttgccacc attttttttc agtatcaatt tgtttgtcta ttgtttctgt tgcttcagaa
     1621 agagtcgttg agtcattctc tggagcttct tctgtataag tcaactgtag tttttcttgc
     1681 agtttttcga tttgtttgtt ggcttgtagg gtgagttgtt gttgctggtc gagtagtttt
     1741 tgtatatgag aattattttg ctctctgagg gcaagttgtt ctcgtaaaaa ttcattttct
     1801 tcacgcaact gatctagcaa cttgtttgta accacgtttt tgcttggtat attaacattt
     1861 ttatcgttgt cttgttcgta ctcgtgcaac ctatctagca acatttggtt gcctaagttg
     1921 cgagcaagtt gcaatactcc agaacgcaat acaaccttat aaccacgctc atttttagct
     1981 atcatggaag aatctaattt ttggatatgt ttcgatattg cttgcctact catcttaaaa
     2041 agttctgaca attcctttat tgtaaaggtt tcttgtggtt gcatactggt tgcctcctag
     2101 gttgctactg gaataaatta tctaaatcag ataataaact ttttttctct tttttcgaaa
     2161 gtgcggactc tgtttgtttt tcaagagctt ctttctcttt ttctagttgt ttgatttgtt
     2221 ggaggagttc aagttctcgt ttcttctgtt tgattttttc gttaattgtt gtagcaggtt
     2281 cttcgtattc tttttgttta tcagctaaag ccaatgcttt attttgttct tctttggttg
     2341 gtgcatgttt attaaaatca tctgcttttt tgatttctgg tttaaacgta aattgatagc
     2401 caacgacagg ttttcctcgc ccttttccgt atttcttttt gattgataat cctttgaaga
     2461 ttgcggttaa ttcctgtcgt ataggcttta taacacgatt atcaacgttt gcttggttgt
     2521 tccagtaact ttttggaata tctaatagtt catgaaaatc ctctttagaa aaataagcaa
     2581 aacctttagt tcgatattgt tttagtaatc gaaacatagt ctttgcatag ctactttgta
     2641 attcgttaaa ttgttggagt gaatagcgaa cccactcatc taaattgttt agtaaaggta
     2701 aagctttttc gtaaacttgt atgtctacat atggatcttt agcagttcca cgtattttaa
     2761 actcactaaa cataacgaat ctatctatat cgagcccatt agcacttctt ttaccaaaac
     2821 gtaaatccat taatttatca taagtcattt ctaaatcatt aatgaaacga ttattagcag
     2881 ttgctttata attacttaaa tcttttagtt ggtcaaaagt aagacggact gttttatcgc
     2941 ctttttcgcg cattctagac acaatagaga aaaatagatt catttcaact ggtgtaaatt
     3001 tccgcaacgg aatggtattt aattcatgat ggtatttcac gatttcattt gccaaagata
     3061 ttcctcctta ctccttcaat ggatacagac gttataaaca acgttttgtc tgtgataatg
     3121 catattataa catacgtgag tgcaaaaaaa gagtttaaaa gcagacaaaa tgtagtttaa
     3181 aagcagacaa aatgtagttt aaaagcagac aaaatgtagt ttaaaagcag acaaaatgta
     3241 gtttataaaa cgctgtatcc attgaaggag taaggacaca gccgacggta aagaggttta
     3301 aaaaaaggtt taaaaagaga ttataaaaga gggtgacaaa atttcccgcc taaaaaccac
     3361 ttcttttttc taccaacagg ttcgcgcatt tttcaaaact gccaattcaa aaccttgctc
     3421 cctacggtcg ctaaaaaagt gtgatcgcta tgctctcaca ttgtactgcg cagtcgccta
     3481 aaggctcctt tgctaaaaac aagtcagtaa cggaggtggg ggaagcctct gatagctcgt
     3541 ctaagtcgtt ttaagacagt cttgggtaaa aaaccttatc ttgttcttaa agcgccttac
     3601 aggcggtcag ggcaagccct aacaacccga aaataaggga tacttaaaaa caccacacag
     3661 attttatttt gtaaggtata ataacttgtc gcctatccga cagtcttgcg actggaagga
     3721 ggtgtatcta atgtttgaac ggttcatttg cccccttctt gtagaagttt gtgtctcttt
     3781 attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc gacatcaacc
     3841 tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgatc
     3901 atttgcaggt tcagtttaac atgattgcta atcaaattca attttgttgt tgctgatcca
     3961 accattcctt gacactttct agataattta atgccgtttc ttttttcgtt ggattttgta
     4021 tgtttgttgc ttgctgaagc agtgtttcat aacgttcgta tgtgaccgat tgttgccgtt
     4081 cttttcgttc ttgttcactg gttgaaaaag ggattgtatc gctaaataag ttacaacaag
     4141 cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt ttttacttgc tcatattttt
     4201 cttgtacctc tttttgctgt ttttctggta agttttgata aaaacgagtg agttttttca
     4261 tgtttttatc tgttaaacgt ttctcttgat ccacaaaaat attgttcatg tcctcataac
     4321 aagcggtaaa ttcctccatt ttttgtgcta atgcttgctc ttttttcttt ttatcacatg
     4381 ctgcttcata cttttgagtg atatccccta ataaaatgct tcttgaaaca cgtttattac
     4441 cctctaaaac gccgaataaa ccacttaaaa cgattgataa ggcaaatata accccgacca
     4501 ataaaaatcg cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct
     4561 tatagctaag ccaaataatt cctaaaatac tggcagaaat gagtagatag ggtaaataga
     4621 atgccatgtc tgtttattct ccccatacca accaaaaggc taataatgtt agtactaagc
     4681 aacataaggg ggcaagaata tccatcgatc gttgaattaa agcctttttt tgttcttggt
     4741 ttcgctggtc taatagcgct tggtggagtt ttttgacttg tgaatccatc gtagtttgta
     4801 tcctttcttg ggtataaggc gcaagaaagg cacgttgtct ctttatttcg ttttcgatgt
     4861 cttttaacgt tttagagcga taattcttgt tggtggtctg ttcctcgttc aacgtgttgt
     4921 tcatggtttc ttcgagtcgg tctaatttcc gattgttcag gcgtaaaagt tcgttgttct
     4981 cttcgtactt cttctgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt
     5041 cgttgttgat tgtaggttcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg
     5101 taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggaccaa ttcgtaagta
     5161 aaattttttc cgcgattcaa gcaatgaatg ccgaatgttt taagtgaatc acaaaattgg
     5221 gtatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct
     5281 ttccaaaccg gttcattttt ttcttttaat ttaatttcag acatggttcg tttttcggtt
     5341 ttctttcctc gttccgtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca
     5401 taacatttgt ttaaaaatgc ttgcccatgg gcttgaaatt ttttcccagt ttcaagatta
     5461 acggagttaa tgacaatgtg attatgaata tggtctttgt ctgtatgtgt gtaaatcgcg
     5521 atttggtggt taggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca
     5581 gcggtaattt ctcctggtcg aaaggactga atcaataagt gtgcttgtac acgatcgtct
     5641 tttccataaa gcgtgcgtat ttgtttcatt tcacttttgg cataatcaat gtcacagttt
     5701 attcctgttt tgacggttgc tcgtggttct gcgtaattga ttgctcggct acatgaagtg
     5761 gttcgactta ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt
     5821 tttcggataa acgttgagca tcatcaggag caacgtcgtg ttggtgtgta ttgcaccact
     5881 tggcaatttg attgatattc cctccagctt tggctaaatc gcgcgcgatt gcctgggcaa
     5941 tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag
     6001 ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc
     6061 tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta gaatgatcgc
     6121 tagccaaatt ttgatgttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg
     6181 tatcgttcgc aataccattg tacctaaaaa ggtacaatga gtaaagctca ctaaaagggt
     6241 ttggcaagct ctatatttgc tagccaaata cctccgtatt tggtgcaaat aaccgcttgt
     6301 caa
//
