LOCUS       NZ_LR135371             1928 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8195 plasmid 8.
ACCESSION   NZ_LR135371
VERSION     NZ_LR135371.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885624
            Assembly: GCF_900639565.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:57:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 38 of 113
            Pseudo Genes (incomplete)         :: 72 of 113
            Pseudo Genes (internal stop)      :: 23 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135371.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8195"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            201..1166
                     /locus_tag="EQB49_RS16190"
     CDS             201..1166
                     /locus_tag="EQB49_RS16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002305967.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVGK
                     LNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHP
                     DKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPV
                     SFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRG
                     KATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLL
                     KEESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1366..>1548
                     /locus_tag="EQB49_RS16195"
                     /pseudo=""
     CDS             1366..>1548
                     /locus_tag="EQB49_RS16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          400..1166
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtaaaaaaa tagtcgagca gtgttgacgc actctcgacc tttacggaaa ctacttcacc
       61 tagttccgta actcaagttc attatacgaa tatttttagg tttcttcaag ctagagattt
      121 ttggatgtgg atttttagag ttacgtgaac tctggaaatt ctttccaaga atctcttttt
      181 tcgttgagga ggagctattt ttgagtaaaa aaggcataat cgtaaatgag tgggaaaaac
      241 ttaaccgtat ttctaacagg cggttaagtg ttgacgataa ggtacacccc ctaaaattaa
      301 gttggtcaat cgatcgaatt acaatcgtcg gaaaactgaa tgataccata gcttattgga
      361 cgcaaaatgg agttgtgttt gtcgattttg agcaattaat gcgtttgaat gaaaatcaag
      421 ggtatttgaa gtcggttggt acaaatggtt ggcaattatt tgaccaatat ggagaagata
      481 tcgcctatat cgaaatgtta aaatttcacc ctgacaaagg tcgtattgat tttaatccaa
      541 ataaaattgg cgagttttta aaggggtcga tgaaacaatt cattcatgat ttgtttctgg
      601 atgcgcattt ctcacgtgca gatattgctt gcgatattct cggtgttcct gatgatttta
      661 ttacccagta tcgaatcgtt gaccctgtgt cttttaagcc aatttatggc cgttctggca
      721 aattagaaac agcgtactgg ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca
      781 aaaagcttga acaggaacga aaaaaacaga ttgtaccaaa agaaatcgaa acatggtggc
      841 gtatagaaat gcagctaaga cgtggcaaag caacagattg gcatgcgatg gttcgggaaa
      901 gtttagatag ctttgcaagt cctcattact tgcctggaga tgtaaaacct gttgatagaa
      961 ttatgataaa agggctgaat caagatcatt cagagtgggc gtatattagc agaaatttga
     1021 aatatagatt gcgcaaattg ttgaaagaag aaagccaaaa cgatgagttg acgaatcatt
     1081 tacgagagac gtttaaggaa tctgcaaacg acttgaaaat agaattagac acgtggcttt
     1141 tgggtttgga cgtgacagag aagtgatacc gaataagaaa agtcagatga gtgagagtaa
     1201 atttacgtca atgtttaatg acgtgagttt acgataagcg attattttgc gtgaagcaaa
     1261 ataaagcgta aaaggagttg agagcgtgac acgctttcgg ctcctttttt cgttgtcact
     1321 tgtgacagcg aataccctcg gagagcattt ggaattgtga cagccatgtc acaattagga
     1381 aaatgttact ctttgtcaaa gagtaacaga aaggtttaca ctctttttat tagcgacagc
     1441 gcgaaggagg ggtttaactt ggggtttagt atttcgttca aaaagggaac aaaaaataca
     1501 agcatttctc ataataatcg tgagttaact gatagccaaa aaatcaatag tttgtctggt
     1561 agaaaataat tagcagattt acaagtggga aataaaaagc tgaaagaaga aaattgcatt
     1621 tcaaactata ttttctacaa tagctctaaa atggcatatt ttgcgttctg agcggtttta
     1681 gcgtttattt cgtttagtta tcggcacaat cggtgaaaca cgttagagga gcgaaaatgg
     1741 agcctgtaag gcgactggta gcggaacggt cgtgttagat tgtgtagccg atagctgaac
     1801 gaaataaatg ctggcagcgc cattctattt cggcaggagg aggctcaagg ggttcgggga
     1861 atgaaattcc ctgaggtctt tttatttttt ctagaggagt gctataatca aagaaaatga
     1921 gaaaaaag
//
