LOCUS       NZ_LR135370             1981 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8195 plasmid 7.
ACCESSION   NZ_LR135370
VERSION     NZ_LR135370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885624
            Assembly: GCF_900639565.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:57:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 38 of 113
            Pseudo Genes (incomplete)         :: 72 of 113
            Pseudo Genes (internal stop)      :: 23 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135370.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8195"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            135..1151
                     /locus_tag="EQB49_RS16180"
     CDS             135..1151
                     /locus_tag="EQB49_RS16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002299382.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGTT
                     TPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQLF
                     DQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIACDI
                     VDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERKKVIV
                     PKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGLIAEPS
                     NWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1351..>1533
                     /locus_tag="EQB49_RS16185"
                     /pseudo=""
     CDS             1351..>1533
                     /locus_tag="EQB49_RS16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          136..1145
                     /name="rep_cluster_935"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.292"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctccgtaact caggttcatt atacgaatat ttttaggttt cttcaagcta gagatttttg
       61 gatatgaatt tttagggtta cgtgaactct ggaaattctt tccaaaagtc tcttttttcg
      121 tctggaggac gaaaatggca aatgaaaatc agttgagtga aattcaatca aaaattgaag
      181 gtcgaggggc agaaaaaaga acaacgataa atcagctcga aaaacttaac cgtatttcta
      241 acaggcggtt aagtgtgcaa ggtacgacca cccccctaaa attaaattgg tcgattgatc
      301 gaattacaat tgtcggaaaa ctgaatgata ccatagctta ttggacgaaa aatggagttg
      361 tttttgtcga tttcgagcaa ttaatgcgtt tgaacgaaaa tcagggatat ttgaaatcag
      421 ttgggataaa cggctggcaa ttattcgatc aatatggaga agatattgcg tatatcgaaa
      481 tacttaaatt tcatgaagga aaaggtcgta ttgattttaa tccgaataag ataggccagt
      541 ttttagctag ttccatgaaa ggattcattc atgacctatt tttagatccg catttttccc
      601 gggcggatat tgcctgcgat attgtagatg tgccggatga ctttattact cagtatcgta
      661 ttgtagaccc gatttctttt aagccaatct acggccgttc tggcaagcta gaaacagcgt
      721 actggggcag tcgtgccagt gagcgccaag tgcgactgta caacaaaaag ctagagcagg
      781 aacgaaaaaa agtaatcgtg ccaaaagaaa tcgatacgtg gtggcggtta gaaatgcagc
      841 taagacgtgg caaagcaacg gattggcatg cgatggttca ggaaagctta gatagttttg
      901 caagcccgca ttttttgccg attgatataa aacctattga taaaatagtt attgatggtt
      961 tgatagctga gccttctaat tggtcgataa ttgctagaca tacaaaatat aaatatcgga
     1021 atttgctgaa gcaagaaagt caaaatgatg agttaacgaa tcatttacga gagactttta
     1081 aggaatctgc agacgaatta aaaaaagaat tggatacgtg gcttttgggg ttggatgtga
     1141 cagagaagtg ataccgaata agaaaagtca gatgagtgag agtggatttg cgtcaatgtt
     1201 tagtgacgta aattcacgat aagcgaatat tttgcatgaa gcaaaataaa gcgtaaaagg
     1261 agttgagagc gtgacacgct ttcggctcct tttttcgttg tcacttgtga cagcgaaatc
     1321 cctcggagag cgtttggaat tgtgacagcc atgtcacaat taggaaaacg ttactctttg
     1381 tcaaagagta acagaaaggt ttacactctt tttattagcg acagcgcgaa ggaggggttc
     1441 accttagggt ttagtatttc gttcaaaaag ggaacaaaga atacaagcat ttctcataat
     1501 aatcgtgagt taactgatag ccaaaaaatc aatggtttgt ctggtagaaa ataattagca
     1561 gatttataag tgggaaataa aaagctgaaa gaagaaaatt gcatttcaaa ctacattttc
     1621 tacaatagct ctaaaacggc atattttgcg ttctgagcgg ttttagcatt tatttcgttt
     1681 agttatcggc acaatcgtta aaacacgtta gaggagcgaa aatggagcct gtaaggcgac
     1741 tggtagcgga acgatcgtgt tagattgtga aagccgatag ctgaatgaaa taaactgcgg
     1801 gtgcgccctt tccatttcgg caggaggagg ctcaaggggt tcggggaatg aaattccctg
     1861 aggtcttttt atttttttag gaggagtgct ataatcaaag aaaacgagaa aaaagagcaa
     1921 aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact acttcaccta
     1981 g
//
