LOCUS       NZ_LR135369             3199 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8195 plasmid 6.
ACCESSION   NZ_LR135369
VERSION     NZ_LR135369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885624
            Assembly: GCF_900639565.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:57:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 38 of 113
            Pseudo Genes (incomplete)         :: 72 of 113
            Pseudo Genes (internal stop)      :: 23 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135369.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8195"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            1088..1807
                     /locus_tag="EQB49_RS16160"
     CDS             1088..1807
                     /locus_tag="EQB49_RS16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(1944..2360)
                     /locus_tag="EQB49_RS16165"
     CDS             complement(1944..2360)
                     /locus_tag="EQB49_RS16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2363..2647)
                     /locus_tag="EQB49_RS16170"
     CDS             complement(2363..2647)
                     /locus_tag="EQB49_RS16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            3013..>3199
                     /locus_tag="EQB49_RS16175"
     CDS             3013..>3199
                     /locus_tag="EQB49_RS16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232043308.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRSL
                     EKLMQLVNEDGGISDAA"
                     /name="hypothetical protein"
     Typing          1089..1807
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgacgcatg gagcgaattt catggagaat ataattaagt gggtttaaat ggctctaaaa
       61 tcgcttctaa gagtttttta gcttggacca atgaaataca ccaaaaacta aataaaacgt
      121 cttaaatcga aaaatacgag ctctcagagc gactttattt gtaaatgttg catataagaa
      181 ataattacgg attttagact tttgtaggat atttttgaaa taaatattta tttctgttat
      241 aatttgattg caaataagtg aacacgaaac acacgaaagc cccagagagg tcggattctc
      301 tggggctttt tttgtgggct gagtcgcctt aattaagttt tacttcttgt ttcggttatt
      361 caaccagtaa ctgaacaaag cgcttaaaag cccaacaaga agggagcaaa taaagttagt
      421 gaacacgaaa cacacctcct tccagtcgca agactgtcgg ataggcgaca atcaattata
      481 caataaaaaa gcaaaaataa gcctaagttt tttattgcct tggggttgtt agggcttgcc
      541 ctgaccgcct gtaaggcgct ttagaacgaa ggacatagtt tttatcgaat cttgtcttag
      601 aacggcttag acgagctctc aggacgccac aaaggagtcc ctaatgaatt atttttggcg
      661 aaggagccta aaggcgactg ctgcaagtaa aatgtgagag cgtagcgatc actccttctt
      721 acagcgacca tagggagcta gattggcagt ttaatatcat gcgcgaacct gtttatttta
      781 aaaagtgggt gattttttgg cgggaaattt tgctcgcgcg cgcgcgttta ttttatttat
      841 tttttaaaga ttttttagaa ccctttttag ggggcagttg agccttactc tcccaacgga
      901 tacagaaggg tactaacaac tttttatctg agtactaaca actttttatc tgagtactaa
      961 caacttttta tctgagtact aacaactttt tatctgagta ctaatatcct tttgtataat
     1021 aacaaccaac ctatttacga gttggttgtt aaaggttatt atttatttgt aaggggggaa
     1081 gaaaggaatg gatgaaaaaa cggaattaac agtcaaatat caaaatgaat tgaatttagt
     1141 accgctgaaa aatttcaatg ctaagcagat ggatttattt tttgcattat gcgctcgtat
     1201 gaaagatcgt ggtttgaaaa aagttagatt ttctttcgag gaattaaaag agttaagtga
     1261 ttataagatg actgctacaa aagcctttgt cggtgattta gaaaagctct ataaagatat
     1321 gcttaatctt acttaccgta ctgaagatga ggattctatt cgctactttg ttttgtttac
     1381 tggttttgaa atcaataaaa caaaaaagtt tgttgaagtc agtgttaatc cggacctaga
     1441 atatattatt aatggtttaa cagctgaatt tagtagattt gaattatctg ctttcacgtc
     1501 gttgcgttct acgtatgcaa aaactctatt ccgactgctt atgcagtacc gttctactgg
     1561 ttattatgta gttcgtattg aagagtttag gagcttaatg gatatcccag aatactatca
     1621 aatgggtaac attgatcaaa aagtattaaa tcctgcatta cgagaactaa aaaaatactt
     1681 caatgatctt aaagtcacaa aaataaaagc aaaaaagggc aataaaatcg ccaaattaga
     1741 atttactttt tcaagcctaa cgtcagattt gccaaagatt tctctgcgta actggttaga
     1801 agaataaata aaaaagaact cgcccccctt acattaagca agtagacaag tcccttacaa
     1861 ccgactaaga ttgctagtac attctagcat attgtatggg gcttctaaat aaaaaacaga
     1921 taggaataac tcctatctgt tttttatcgt tttagaaaat ttgttgggtc gaaatttcta
     1981 gaagtgactg tttgactggt ttctttttga tgattctttg gtcgatttcg atttgatgga
     2041 tttagtttag cttcgatttt ttctttcact tcttgatata ctttagaatt tgtgccaata
     2101 agaacgcctg tatcttttag atataaatca gcaaattctg cgatgatttc agtcatgatg
     2161 gaaatttctt ttcccatagc atttagtttt gcctgattgc tggtagtggt cgtgttggta
     2221 tcgggttcat aactcataca taaaaagcga atggcttccg agtagttatt gatatctgga
     2281 tttttttcga tgatggaatc taaaacgtca acatgactag ggtacaaagt aacatgtttt
     2341 tggacagaag tttttctcat ggttatatca ttcctccgat aagcatttca atgatttgat
     2401 tatttgtgta gacatactcg cttaatattt gagtattttc tgtttggata gcaataaatt
     2461 tatcgagcaa taaatcgaat tggtcttttt tgtaagtcgt aagttgacgg tcaaaatcac
     2521 tttcgattaa aacttttaga tatttatttc gactccattt ttctcctgtt ttattcgtga
     2581 gttcttggca acgtttgtct attgttgcaa caaactttgg atcaatattc cgaactaaaa
     2641 tgtccataaa aaatctcctt ttgcaattat gtataattta tgtatatcat acaaatacta
     2701 tatttgtata gctgtttttt gccaaaatgc tatacataag ctacccacga tggcgaaaaa
     2761 gtgatatcaa atttttttct gagaaacgtg ataggcacag cctatctacc cacttttttt
     2821 agaaagtggg tacacgtttc cttatgctct ttaaaattcg ggggggagtg ctgcacgtgt
     2881 aatcgataac caaatttaaa aatgcacgat caaaaattta tttagttttt caaacgacat
     2941 tttttttgag agtatagacg gcaaagaagt gattttattt ctagaaaaat tgtgagactg
     3001 gagaaagaaa aaatggaaaa atttccttac gaatattttc aaaacttaga aaaattagag
     3061 caagcaattc aagcaatcaa gttcgattta acagacgaaa aattgttgga atataaaaat
     3121 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     3181 ggaatttcag acgcagctt
//
