LOCUS       NZ_LR135367            15151 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8195 plasmid 4.
ACCESSION   NZ_LR135367
VERSION     NZ_LR135367.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885624
            Assembly: GCF_900639565.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:57:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,105
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 38 of 113
            Pseudo Genes (incomplete)         :: 72 of 113
            Pseudo Genes (internal stop)      :: 23 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135367.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8195"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            194..847
                     /locus_tag="EQB49_RS16015"
     CDS             194..847
                     /locus_tag="EQB49_RS16015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_224813426.1"
                     /translation="MKRTWGKNLTILMLGIIITGCGEKSQETAKETKAYTFAVSTTVQS
                     LDPKLAIDPTSQLVLNNVMEGLYKLNAKGEIVPGGANGLPEISKDGLKYVIRLRKDAMW
                     SNGTPVVADDYVYSWKRTLSNQDSPNIGFFESIKNGKEIISGTMKPDKLGVYAKDKNTL
                     VINLSTPIPYFTSMLINTAFFPQKQEVVEKEGDRYGTNSDSVIYNGPYIGVHGT"
                     /name="ABC transporter substrate-binding protein"
     gene            complement(892..1497)
                     /locus_tag="EQB49_RS16020"
     CDS             complement(892..1497)
                     /locus_tag="EQB49_RS16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002321606.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERPE
                     FQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNEL
                     LDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRL
                     VFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
                     /name="recombinase family protein"
     gene            1622..1909
                     /locus_tag="EQB49_RS16025"
     CDS             1622..1909
                     /locus_tag="EQB49_RS16025"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="WP_060763464.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPAT
                     RLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIGSVAKF"
                     /name="DUF4158 domain-containing protein"
     gene            complement(1943..2623)
                     /locus_tag="EQB49_RS16030"
     CDS             complement(1943..2623)
                     /locus_tag="EQB49_RS16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338443.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_010710189.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPD"
                     /name="IS6-like element IS1216 family transposase"
     gene            2748..5099
                     /locus_tag="EQB49_RS16035"
     CDS             2748..5099
                     /locus_tag="EQB49_RS16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="WP_000344083.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQV
                     ELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVYTP
                     YLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLNVNY
                     LEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGNSGNY
                     VRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKGLLSSA
                     GDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTTEELNNI
                     FSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAVDLDMLGE
                     EILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMSTGYSGNLG
                     FPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNYIAKYNQKVI
                     NQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRKIYDEYRNKLT
                     NSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWLGDLMTCWGEVK
                     KLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDSTALSALKTYYCDK
                     GAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFIDKIVNNSLGTDFT
                     YLDFIGDEGKVANGFSEKIIQFQENRSKLN"
                     /name="glycoside hydrolase domain-containing protein"
     gene            5179..5574
                     /locus_tag="EQB49_RS16040"
     CDS             5179..5574
                     /locus_tag="EQB49_RS16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002304891.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVD
                     YFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKE
                     YNYIVKAKVSKRFIFNPKLEIYSVVIE"
                     /name="hypothetical protein"
     gene            5899..6516
                     /locus_tag="EQB49_RS16045"
     CDS             5899..6516
                     /locus_tag="EQB49_RS16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001224538.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFKR
                     PEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTASY
                     KQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPPEFP
                     AIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
                     /name="recombinase family protein"
     gene            6557..6856
                     /locus_tag="EQB49_RS16050"
     CDS             6557..6856
                     /locus_tag="EQB49_RS16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="WP_000366408.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFLV
                     CGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
                     /name="BRCT domain-containing protein"
     gene            6890..7087
                     /locus_tag="EQB49_RS16055"
     CDS             6890..7087
                     /locus_tag="EQB49_RS16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048669782.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCPK
                     CHRRYKQKQGEYVAITIIKE"
                     /name="hypothetical protein"
     gene            7390..7722
                     /locus_tag="EQB49_RS16060"
     CDS             7390..7722
                     /locus_tag="EQB49_RS16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="WP_010714745.1"
                     /translation="MPLTLILGGEYIYYDKNLGMMVAGDNSNSELIEQSDNVPLDRHKC
                     KEHVFKDKRFVEKESESLTLLGYKIVVDTVTGVNYLETPKGGITPLLDKNGKICIDKKK
                     SINKKG"
                     /name="DUF6440 family protein"
     gene            complement(7755..8039)
                     /locus_tag="EQB49_RS16065"
     CDS             complement(7755..8039)
                     /locus_tag="EQB49_RS16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010714377.1"
                     /translation="MSSLINRKIPQGATANKEADTKRKVTIQKKSSKKILASERKNIKV
                     SPSTFDLIKTISTMKSYKNYEFIDKAVESYIQNNLTEREQRILKNLISK"
                     /name="hypothetical protein"
     gene            complement(8032..8922)
                     /gene="prgP"
                     /locus_tag="EQB49_RS16070"
     CDS             complement(8032..8922)
                     /gene="prgP"
                     /locus_tag="EQB49_RS16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA superfamily DNA segregation protein PrgP"
                     /protein_id="WP_010714376.1"
                     /translation="MANTIIFGQQKGGVGKTTDTCMITLVAAMLMKKRTILIDIDLQAN
                     ATNFMTKTFGVETVPQTLMSALENGDLRPAIVNLKENLDLIPAGYDMRLYTEFLTENFD
                     NTIDRTFYLKNLIEEIKDDYDYIFIDIPPSTDLKVDNAMVACDYLVVVQETQQFSFEGS
                     QRLIFDYIQTLVDDFGSDVPIQIAGILPALLQQKRPMHQKLVEETIDTFGRDNVFNTII
                     QNHARLEWYPRIGFQFQDYHDKKMIALFADIFCELEERITLYKSNGDIQDYFYTPRYIL
                     DGKLTSLGKEISLNV"
                     /name="prgP"
     gene            9626..11131
                     /locus_tag="EQB49_RS16075"
     CDS             9626..11131
                     /locus_tag="EQB49_RS16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_060763466.1"
                     /translation="MKVSDVISVILKSGIREYKCLNSKIKPIGYKAEGKKGAIFGFRSK
                     KLMTESRGIVLTSQEGLAENEDKFTHWTPNVYSYGAYADEKRIIVKGHNERNLQQINTF
                     VIDFDRLTGEKLDSQMILDAAIDLELMPTLILETPGGFQAYFILENAWYISSKNNYQSI
                     EVAKRVSENLRKAFAEELPSVDLGCNHFGIARIPRTDNIVYYYPALTHDIQQLIQWSMK
                     YEPAKTYKKPLLSLVKNKYKQVNEMWYRQLISNPKIVGAKGIMGRNNVIFTLSLANYAS
                     GVDLEDCLNEMDLFNSSLERPLKDRERVRIVKSAYSGKYRGASKTYVQELVSNWGLESL
                     SEEKMFTQQRNTWYKFKKERHKRKKSHLSEWKEDILAYLETQCYRYRPEVRLKKTDLQA
                     AITFNGQSIPKRSLDRALKELMAEGKLFVQIKAGRNGGLVVATRKALIRTVIQVKQQVK
                     SAYKQGIKTFFKEADMLVRIFREPDKYSQRVDHYKQLNLWNTG"
                     /name="primase C-terminal domain-containing protein"
     gene            complement(11258..12580)
                     /locus_tag="EQB49_RS16080"
     CDS             complement(11258..12580)
                     /locus_tag="EQB49_RS16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="WP_060763465.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKLY
                     KHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKSIQ
                     EQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLV
                     LTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNPILPHAKVV
                     VDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNWH
                     VEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFNSS
                     AFNKAMLYDTFNWYEVNDHDITDNFGVDFSTPIKNLEKGDL"
                     /name="ISL3 family transposase"
     gene            complement(12740..14635)
                     /gene="merA"
                     /locus_tag="EQB49_RS16085"
     ARG             complement(12744..14635)
                     /name="merA"
                     /gene="merA"
                     /gene_name="mercury(II) reductase"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="96.04"
                     /coverage="100.0"
     gene            complement(14664..15056)
                     /gene="merR"
                     /locus_tag="EQB49_RS16090"
     CDS             complement(14664..15056)
                     /gene="merR"
                     /locus_tag="EQB49_RS16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /GO_function="GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="WP_001863748.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYDD
                     VKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKRIE
                     TMLDDLKQRCPDEKQLHSCPIIETLT"
                     /name="merR"
     Typing          9627..11112
                     /name="rep_cluster_180"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.832"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgattgata cttttatata atactattga ttatcctaac atcccaccag aaaatccgat
       61 ttaaacacca gttgttttta aactattaag aaagtaattc cactatatat tccgaagacc
      121 cgtttttttt acagtataat taagctaagt gttgctagaa caatcagata agaaattgaa
      181 taggggtgtt tttttgaaaa gaacttgggg aaagaattta acaattctta tgttaggaat
      241 aattattact ggttgtgggg aaaagtctca agaaacagca aaagaaacca aagcttatac
      301 ttttgctgtt tctactacag tacaaagctt agatccgaag ttagctatcg accctacaag
      361 ccaacttgtt ttaaataacg taatggaagg attatataaa cttaatgcta aaggtgaaat
      421 tgtacctgga ggagctaatg gccttccaga aatctctaag gatggactta agtacgttat
      481 tcgattgaga aaggatgcaa tgtggagtaa tggtacgcct gttgttgcag atgactatgt
      541 ttattcgtgg aaaagaacgt taagtaatca agattcacca aatattggtt tttttgaatc
      601 tattaaaaat ggtaaagaaa taatctcagg aactatgaaa ccagataagt tgggagttta
      661 tgctaaggat aagaatactt tggttattaa tttgagtacg ccaatcccat atttcacttc
      721 tatgctaatt aatacagcat tttttcctca aaaacaggaa gttgttgaaa aagaaggaga
      781 tagatatggt acgaactctg atagtgtgat ttataatgga ccctatatcg gggtacatgg
      841 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
      901 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
      961 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     1021 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     1081 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     1141 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     1201 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     1261 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg atgtaacgat
     1321 gacgcaatca ccttcacgga tataatttag taatttttga aactcaggac gctcagtatc
     1381 tttaccactg gctttttctt ggaataattt atcaatgtta agtccttcca tcattgccaa
     1441 ttgtcgatcc aaattttgat cttgtgaaga tactcgaata tagccaattt ttcccataaa
     1501 aaactccttt ttaaaagtga tataatttac atgtaaaatt tacttgtagt tttactgcta
     1561 tcatagcatt tttaaaaata aattttatgt agacttaaaa tttacttcat ggagggttta
     1621 aatggatgaa atcaaaagta acaaaaaagc atttggcatt tatttaggag aacctagtac
     1681 agaacaatta aaccaatttt tttatttgca tgaaaaagat ttagaattga tggagactat
     1741 acgacgacca gctactagat taggttttgc actacagtta gtctcactac gttttttagg
     1801 aacttttata ttagatctta ccaatattcc ccaaaatgta ttgagttatg tagcaactca
     1861 actaaatatt gatcctggag aaattatcgg ttctgttgca aagttttaaa taaagaataa
     1921 aatcccttac ggtatctatg atttaatctg ggattcccaa taataccttg atttcagtac
     1981 agaccgaaaa cccgaagaga gtgccttctt ttcgggtttt cttatataat cctcggatgg
     2041 cttccatgcc tttaatcgtg ggtgaggcag tgcgtaaact tcgatagaat ttattgcgtc
     2101 tctttactgg acgatggtct tgttcaatca aattattcag gtatttaatg gtacgatgtt
     2161 ctgtcccttg ataaaatccg tattctttta gtttcttaaa ggcactagca attgatggtg
     2221 ctttatctgt gactacaacc ttcggttcat caaactgctt cactaaccgc ttaagaaaag
     2281 cataggctgc ttgtgtgtcc cgttttttac gtaaccaaat atccaaggtt aaaccatctg
     2341 catcgatggc tcgatacaaa taatgccatt ttcctttaat tttgatgtac gtttcatcca
     2401 ttttccagga ataaaaggat tttttatttt tctttttcca aatttgatag agtagtttgc
     2461 catattcttg cacccaacga taaatcgtcg tatgagaaac gttaatgcca cgatcatata
     2521 agatttcttg aacttcacga tagctaaggt tataacgaag atagtagccc acggctacaa
     2581 taatcacatc ctgctgaaat tgctttcctt taaaatgatt catcgtcatt cctcctgcta
     2641 tctttttcta ttattctacc ttatttgata gtagatttaa aactttgcaa cagaacctaa
     2701 tcaacacata aacgaacgta tgttcggcaa gaaagcaggg gatttttatg gatgagatgg
     2761 ttttaagtac tcagaaatgg ctaaacaaga aatacagtaa tgttacaggg tttgataagg
     2821 ttccagaaaa tgggcgaaca gggtggccta caatctatgg acttattgaa ggactacaag
     2881 tagaacttgg tattactaat ctagtagcta attttggacc taccactgaa aaaatgtacg
     2941 ataatcaggt tactcctaaa tggggcaaaa atctacctaa aaatattgta tttcttattc
     3001 aaggtgcctt ttggtgtaag gggattaatc caggtggctt cgatggggta tatactccat
     3061 atttagatac tgctgtaaaa gagttgcaga ctgatgcagg gtttaatgga agcgctgtaa
     3121 ctggagtttt ggatgcgaaa tgggccaaag cgttatttga tatgagtgct tttgttcttg
     3181 ttcccggcgg agatagtaaa attagaaaaa tgcagcaatg gttaaatgta aattatttag
     3241 aatacacggg aataatgcct tgtgatggta tttatcaaag aaattcaaat caagcattaa
     3301 tttttgcatt acaagctgaa ctaggatatt ctcccagtga agcaacaggg tcttatggta
     3361 atgggacaac tagtaaaact tatcctgtgt ctgaaggtaa ctcgggaaac tatgtaagaa
     3421 taattcaata tggattatat gttaatggtt attttgaaga cggtacattc gatggtattt
     3481 tcactaaata tatgggttta gaagtactgg cattccgaaa atttatggta ttgcctgaat
     3541 atactgaaat agcagatgtt gttgtaataa aaggactatt gtctagcgct ggagatatta
     3601 gaagatctgc agatggcgca gatacttcta cgcaattaac tagatctcaa atccaaacat
     3661 tggttgataa tgatataaaa atagttggta gatatcttac tggtactgtt ggaattggaa
     3721 aagatgaacg aaataagtat ctaactacag aggaattgaa taatattttt tctaaaaatt
     3781 tatctgtgtt tcctatctat caagatggag gtgcagcatt agcttatttt acttatgatc
     3841 gtggcttatc agatggtaag aaggctatag atgcagcaaa aaatctaaat ataccattaa
     3901 caacagttat ttattttgct gttgatctag acatgttagg ggaagaaatt cttgcatatg
     3961 ctggagaata ttttaagggg gtatctgctg taatgtcata tagcgggtat caaacgggtg
     4021 tttacggaac aagaaatgtt tgttctcaaa taatcaataa cgggtatgct tcgtttgcat
     4081 ttgtttcaga tatgtcgact ggttatagtg ggaatttagg ttttcctatg cctagacaat
     4141 ggtcttttga tcaatttgtt gaatttacca taggaagtgg caacggagct gttggtatcg
     4201 atttaatcgc tacatcgggt agagatagcg gatttaatga gttatcaaat gatactaata
     4261 atgataatta cattgccaaa tacaatcaga aggttataaa tcagatggta cgtgcgtatc
     4321 aatatctatc acaagctggt cttgataatc cttggaaccc tcatttgaca ttttatagat
     4381 atgtaaatta ttctggtttg tcatgggaca tcatttcttc tcctgtaaca gagcatgata
     4441 gaaaaattta tgatgaatat aggaataaat taactaatag cgagggcctg tataattatt
     4501 ttatagatcc taatactggt tcaataatag gtttgcctca cttaattgtg acgttacaat
     4561 cacagatgtt tctagccgac acaatcaatg atagtgtttc tgactttgca ggttggttag
     4621 gagatttaat gacttgctgg ggtgaagtta agaaattagg aatacaaatg gagcaaggtg
     4681 tatttgcatt agtaggtacg gctgcaggtg gaacatttag tctagaagat ttataccaag
     4741 atgttgacgc ggtaaatgtt tataccttag caaaaagaaa ttctgattca acagctttat
     4801 cggctttaaa aacgtactat tgtgataagg gtgctattga tagatttaat caatttgtta
     4861 agaacagatt tggttcttca gataaaatcg cttcagtaac aaatagttta ttagaaaatg
     4921 acgaaacagt tgatatctta ggtgtaggga taatttcatt tttcattgat aaaatagtta
     4981 ataattcact tgggacagat tttacttatc ttgattttat tggagatgag gggaaagttg
     5041 caaatggatt ttctgaaaaa attattcaat ttcaagaaaa tagaagtaaa cttaattaag
     5101 aatcgtttac tatttaaaca gtgtcctttt cctttttaaa tatatacgat aaaataagta
     5161 tatgaattgt aaaaggacat gatgattatg gaagaaatag aatggaataa tctgactcaa
     5221 aataataaaa ataattctgg atgtggaatt atctttatgt ttgttatagt tttgggaata
     5281 gtagtacttt taataactgt caagatagta gattactttg cgaatactcc tgaagaacaa
     5341 atacagagaa aacttcctga gtttgaaaga gtaccacctt atgatccaag agataattca
     5401 tttcaagtaa aaaaattaaa agagtctcca aatgaaatgt ggtttgaagt tacagttgaa
     5461 tcaagcgact tcaaagaaaa aaagaaagaa tacaattata ttgtaaaggc aaaagtatca
     5521 aaaagattta tttttaaccc taaattagaa atatatagtg ttgtaataga ataatagtgt
     5581 taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt gaaaaataaa atataccatg
     5641 gaagacataa aagaaaaact ggataactag tttatttaat ggcagaccgt taaggcctgc
     5701 catttttatg tattttagcg gaataaatat aatcatgcgg gagtgtaaac ttactgtttc
     5761 aaaaagtata ctttttgaca tgccttgatt ttctttgttt atgtatggca taataaccac
     5821 ataatacaat ttgaaacgtt tctgatttaa tctgattgtt ttgaaacaat tttagtgaaa
     5881 aaagggtaga taaaaacgat gagaaaattt ttttatatcc gggtcagtag ttatgatcaa
     5941 tcgatcgaac gacaactgat ttccgcgaag gaattaggaa tccctgaaga atatatattt
     6001 gtagaaaaag ccagcggcaa agattttaaa cgccctgaat atcaattaat gaaacggatg
     6061 tttcgaaaag gagatttact ctatctacaa tcattagata gactaggcag aaataaacaa
     6121 atgattttag aagaatggcg agaattaact aaggaaaagc agattgatat ttctgtgtta
     6181 gacatgcctc tgttagatac tgctagttac aagcaactag atgggttaga gacgcttatc
     6241 tctgacttaa ttctgcaatt actcagctat atggccgaag acgagcggaa acgaattaat
     6301 agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg gtgtaaaatt cggcagaaag
     6361 aaaattgctt tacctcctga atttcctgcg atctattctg actggaaagc aggaaagata
     6421 actgcagtct atgctatgga tcaattgggc atgaaacgaa atacctttta tcgacgggtg
     6481 aaagaatatg aacatacctt gcaccacttt tcttgataga tataacagct attagacggg
     6541 aagggagagt ttgaaaatgg acttaacgaa tcaaagggtc gtttttactg gaacgctgca
     6601 gcaaatgaca cgaacccaag ccatcggttt ggttcactct ttaaacggcc attgtcaatc
     6661 aagtgttact tcaaaaacaa atttcctggt ttgtggtcag tattctaagt cgttgtttga
     6721 tgattctctc tatacaaaaa aggaacaaac cgcgcgggac ttgattgcgt taggtcatga
     6781 gattaccatt ttatcagaag tcgaatttta tgcgttaatt agtcaacagt tgaaagaatg
     6841 gcaacggtat ttataatcgt tagtaaaatt tacaaggagg aaaattttta tgaagcgaaa
     6901 atcttatgat cgttggggca ttgaaataac tagttttaat cgaaatattg cttttcacgt
     6961 ggagtgtgac tgtggggaat tagcaaaaag tgtttatcaa aaaaattact tttactgtcc
     7021 taaatgccat cgccgataca aacagaaaca aggagaatat gtagcaatta caataattaa
     7081 agaataaaca aataagattt acttcaatgt tggtttctgt ttcatactaa ataaaaacct
     7141 attttaattt agtaaaaggg aattggtttg caagcaaaat cttccgtttt ttgatagaat
     7201 taagaaaaag ttgacaacag atcctattta aaaataggat gatttgcact tgaaatttac
     7261 ttattggaat aagaggcaat ctgtttaatc gattgattgt gcgtagaaaa tgaacaaaaa
     7321 ggtaagtaac gaaagccgtt aaaaaaagag aaaaaggacg gtaagaaaat taaagaaagg
     7381 aagattttta tgccattaac cttaatacta ggtggagaat atatttatta tgataaaaat
     7441 ttaggcatga tggttgccgg ggataattca aatagtgagt tgatagaaca atcagataac
     7501 gtaccattag acagacataa atgtaaggaa catgtattta aagacaaacg ttttgttgaa
     7561 aaagaaagtg aatctttaac tctattaggc tataaaattg tagttgatac tgttactggt
     7621 gtgaattatt tagaaactcc taaaggagga attacgcctc tacttgataa aaatggtaaa
     7681 atctgcattg ataaaaagaa atcaataaat aaaaaaggat aaaaagatac atgtatcttt
     7741 ttatcctttt ttatttattt tgagattaaa tttttgagga tacgttgttc acgttctgtt
     7801 aaattatttt gtatatagct ttctacagct ttatcaataa attcataatt tttgtagctt
     7861 ttcattgttg atatggtttt aattaagtca aaagtagagg gggacacttt gatgtttttt
     7921 ctttctgaag ctaatatctt tttactcgat tttttttgaa tagtaacttt tcgttttgta
     7981 tctgcttctt tatttgctgt tgccccttgt gggattttac gatttatcaa actagacatt
     8041 tagactaatc tcctttccca gggaagttaa ttttccgtct aatatatatc tcggagtata
     8101 aaaataatct tgaatatcac cattactttt ataaagagtg atgcgttctt ctaattcaca
     8161 gaaaatatct gcaaaaagag caatcatttt tttatcatga tagtcttgaa attgaaatcc
     8221 aatccgtgga taccactcta aacgtgcatg attttgaata atagtgttaa aaacattgtc
     8281 tcttccaaat gtgtctattg tttcctcaac taatttttgg tgcattgggc gtttttgttg
     8341 taacaatgca ggtaaaatgc ctgcaatttg aattggaacg tctgagccaa aatcgtcaac
     8401 taaggtttgt atataatcaa aaattagccg ttgacttccc tcaaaagaaa attgttgtgt
     8461 ttcttgtact acaaccaagt aatcacatgc aaccatagca ttatctacct ttaaatcggt
     8521 tgaaggtggg atgtcaataa aaatataatc ataatcgtct ttaatttctt caataaggtt
     8581 tttaagataa aaagttctat ctattgtatt atcaaaattt tctgttaaga attcagtgta
     8641 tagacgcata tcataaccag cgggaattaa atctaagttt tccttaaggt ttacgatagc
     8701 aggacgtaga tcgccatttt caagagcaga catcaaagtc tgaggtaccg tttctacccc
     8761 aaatgttttt gtcataaaat ttgttgcatt tgcttgcaaa tcaatatcaa ttaaaatagt
     8821 acgctttttc attagcatcg cagctaccaa cgttatcatg caagtgtcag ttgtttttcc
     8881 taccccgccc ttttgttgtc caaatataat tgtattagcc atcaatatac tccttccttg
     8941 taatcaaaat gtaataaaag ataaatgtat ctttgtatct tttttctata tttaaatagt
     9001 aatacaaata gaattaattg taaatagata aatgtatctt ttttctaatt atcttaaaac
     9061 atacttattt aatcagatag cattttataa gaaaagttat taaatagact tctaaataag
     9121 gatacaaaga taaaaagata aaaggataca aagataaaaa gatacaacaa taaaattaaa
     9181 ggataaaaag ataaaaagat aaaaagataa aaggatacaa agataaaagg atacaaaaat
     9241 aaattaagaa tgattatttg agagaaaatt tttactttac aaaagttgta gtgacatgta
     9301 ttctaaagaa gaatttgaat gaatagaaaa aagatacatt tactaattga attatagaca
     9361 aaaaaataga gacggtaact tagttgattt gccgatcaac taaattacag aatcaaaatg
     9421 tacacagcat ttcatgattc tctttcgtcc cattatagtt ttagattaca gagagttgca
     9481 gctctcttag tctttttaat tatatcattt gggaccttaa aaatcaagat tttgagctat
     9541 attttagctg caaataaggt ttaaaggaga gctgtagaat gcctgcagtt ctcctttttt
     9601 taaatataga atgggtggtg ataagatgaa agtttctgac gtgatttcag ttattttaaa
     9661 aagtggtatt cgtgaataca agtgtttaaa cagcaaaatt aaaccaatcg gctataaagc
     9721 tgaaggaaaa aaaggtgcta tttttgggtt tagatccaag aagttaatga cggaatctcg
     9781 tggaatcgtt cttacttctc aagaaggctt agctgaaaat gaagataaat ttactcattg
     9841 gacacctaac gtttacagct atggtgcata tgcagatgaa aaaagaataa ttgtaaaagg
     9901 gcacaacgaa cgcaacttgc aacaaatcaa tacttttgta atcgattttg atcgcttaac
     9961 aggcgaaaaa ttagattctc aaatgattct cgatgcagca atcgatctgg aattaatgcc
    10021 aacccttatt ttggaaacac cagggggctt ccaagcatac tttattcttg aaaatgcctg
    10081 gtatatttcg tccaaaaata attatcagtc aatcgaagtg gccaaacgag tatctgagaa
    10141 cttaagaaaa gcatttgctg aagaattgcc ttcagtcgat ttaggttgca atcattttgg
    10201 gatagcacgt atccctagaa ctgacaacat tgtctattat tatccagctt taacacatga
    10261 tatacagcaa ttaatccaat ggtcaatgaa atatgagcca gcgaaaacat ataaaaaacc
    10321 gctactttca cttgttaaaa ataaatataa acaagtgaac gaaatgtggt atcgacaatt
    10381 aattagtaat ccaaaaattg taggcgctaa agggattatg ggccggaata atgtgatttt
    10441 cactttatct ttggccaatt atgcatcagg agttgactta gaagactgtc taaatgagat
    10501 ggatcttttt aacagttctt tagaacgtcc tctaaaagat agagaacgag taagaattgt
    10561 aaaaagtgct tactctggga agtatagagg tgcaagtaag acttatgtac aggagttagt
    10621 gtcaaattgg ggcttagaat cgctgtctga ggaaaaaatg tttacacaac aaagaaatac
    10681 ttggtacaaa tttaagaagg aacgtcataa aagaaagaaa tctcaccttt ccgaatggaa
    10741 agaagatatt ttagcttacc ttgaaaccca atgttataga tacagacctg aagtcagact
    10801 gaagaaaaca gatttgcaag cagcgattac tttcaacgga caatcaattc ctaaacgttc
    10861 tcttgaccgt gcattaaaag aattgatggc cgaaggtaaa ttatttgtac aaataaaagc
    10921 tggacggaat gggggactag tagttgccac tcgtaaagcg ttaattcgta cggtcattca
    10981 agtgaagcaa caggtaaaat ctgcgtacaa acagggcatt aagacatttt ttaaagaagc
    11041 cgatatgtta gtaagaatct tcagagaacc ggataaatac agtcaaagag tagatcatta
    11101 taagcaatta aatttgtgga atacaggata aaataaaaca tgtcaagtgc ctatacatta
    11161 tatttctttt atacttgttt tcgtttttgt tagttattat cctaattctg tatatttttg
    11221 tttttttggg tattcggatt ttatggtgga aaagggctta taaatcaccc ttctccaaat
    11281 tcttaatagg tgtggaaaaa tcgacaccaa agttgtctgt aatgtcgtga tcattcactt
    11341 cataccaatt gaaagtatcg tataacatag ctttattgaa agcacttgaa ttaaatcgtg
    11401 gcttacgctt tttatggagc ttctcattga aaaggatttt ggctcgtaag gcatcaaacg
    11461 agtaacctct acccattcgc tctacctgcc taataatgct gttaattgac tccgtataag
    11521 cattagtgag ccttttatca aagtagttga atatttcgac atgccagttg tctacggctc
    11581 tcacgaggtc tttatatgcg tctttagagt tgctggacat acaacggtgt ctccattgac
    11641 tataacgaag atgaccttca tctggatcag gagtatccca tatccagtaa aactcttctt
    11701 tgagttcata ggcttctttt aaagcaggaa gattacctaa ccaagtatct aagaggaatg
    11761 attcacgttc atttaaatcg tgtttacgct ttagaaggat aaacctttca cgcataaggg
    11821 tacgtctttc tttttggctc atatgggctt tcaaagactt cctgacgtta tctaaggctt
    11881 gattagccat tctaactaca tgaaacttat ctacgacaac tttagcgtgt ggaaggatag
    11941 ggttcactgc gtctttgtag ggcttccaca tatccattgt gacgtactca atgtaagtcc
    12001 tgtcactgat ttctgaaaga cgttggatga ctgtttcctt gttacggtta ggcttgatgt
    12061 cataaatagt cctgcgttca atattagtca atacaagccg aggtctacgg ataatatgta
    12121 tctcgtctat cccaagccac ttaggagttt caaactggta ttcacgttct ttgagtgcca
    12181 catagtcctt aaaaacgttc ctaatggttt tctcgtcaac accaacgctt tctgcgactt
    12241 ctacaaaggt cttagacatg gattgctctt gaatggactt taaaagcctt ttggtcatac
    12301 tacgcttctc atctacagat attaggcgtt cccagaaggt agatccgcat tcacgacact
    12361 tgtatcgtct acggttcaat tgtaagccca ctcgctttaa acgaatgggc aaatccataa
    12421 ttagttgatt tcttgaactg tgtttgtaca acttgtcaaa accacattca ggacaacgtt
    12481 caggtggtcc gactgcttca actttaaaca tcatatcggt ttcattttct tgtggcggct
    12541 ctattgtttt aatgtctggt agggataata agtctgacat attattcatc ctttattact
    12601 ctgcggtgtt aaggtatcaa ttatagattt gatgtcgaaa tcatcagtgt taaattattt
    12661 ttgtgaccac aaatgcactt agaaaatccc ttaaagcatt agctggttca cttgaatagg
    12721 gagttgtaaa aaagttccct tagcctgcac agcaagataa tttcgaaaca tctttatcaa
    12781 aagttaggac agccagcttc aatccttctg ccattgttag atatggagcc atcgtttctc
    12841 tcagatctcc aacagttaaa ccgaatttca cagctaatgt tgctgcataa attacgtctc
    12901 ctgcgttttc tgccactaca tgcgccccta acactttcaa tgttttcgcg tctgccacta
    12961 atttgaaaac acctgttgtt tcccgattaa cgagcgctct tggaacagca tccaacggca
    13021 ataccgatgt tttcacttca tatccttttt cttttgcctg ttgctccgtt aaaccaaccg
    13081 ttgcaatcga tggagaagta aacgtaacgc ctggaaccac ttctaaattg accttttgat
    13141 ttagtcctcc gattgcatta cgagcagcaa gtccaccttc ataagcagct acataaacaa
    13201 attggggacc gagagtgaca tctccagctg aataaattcg ggaattggtc gttttaagat
    13261 aatcatcaat gacaatttca ccacgggaac caacttcaac gcctgctgca tgtaagttta
    13321 atgattctgt atttggtttt cttccagtgg caattagcaa ttgttctgct tcaataattc
    13381 gctttttacc atttatctca acatgaactt ttttaatgtc tccatcttgc tcaactcgtt
    13441 cataggttgc acctgttact aaattaattc cctgttctgt taaggcctta gtaatggctt
    13501 ctgaaatttc aggatcgtat tcttttaata gacgctcgct tctttgaatc aaagtgactt
    13561 ctgacccgag gttatgaaat agttgtccta attccatgcc gatatatcct gaaccaatta
    13621 cggtaagacg atttggaacc ttctttaatt ccaataagct agtgcttgtt aaataatcta
    13681 cttcatctaa tccgggaata ttaggtgcag ttgaagaagc acctgtagct attaaaaatc
    13741 ttttggctgt gatttgattg ccatttactt caactgtatt ttcatttacg aattttgctt
    13801 caccttttat taattcaaaa ccataatcat caattaaatt cacatatttt tcatttcgca
    13861 tctcggttac taaatcattc ttttgtttta ctaatggcgc taaatcaaca tttgaagccg
    13921 aagtgtgtaa tcccacaaat ggattatttt ttgctagatg attgatttcc cctgctctta
    13981 ataaggtctt agaaggaacg catccgacat taacgcaagt tccacccacc gttccacgct
    14041 caatcatagc cacttttgcg ttcaaagtaa cggcttcaat ggcagatgaa aaggcagctc
    14101 caccagaacc gatgatgatg taatcataat catagttacc ttcatcattt aaacttacat
    14161 ctgtcctttt ttccgattgc acttgtattt cttctgcttc gcccggttga tattgtgcgt
    14221 cagtaatcgc tgttttcgcg atatcaatgt ctacatcata cggtagttca aacagtgctt
    14281 ctccgcgacg aaagtctact tcaatccctt ttgcacctgc attttcaaga gcaaccgcaa
    14341 tatgttcttc acaaccagtg caggtcattc cttcaacgtt tagccgatat tttttcaata
    14401 aattcgtctt ttgttccgat tgaaattctt ctgcttcgcc cgggtgatag tttgcgtcag
    14461 caatcgcctt tattgcactt tcaacctcaa tatcatcggg cagttcaaat actgcttcac
    14521 cacgacgata actagactca atatttttag cacccatctt ttcaagtgct gattctacgt
    14581 gtttttcaca acccgtacaa gtcattcctg aaatgtttac cttaaattta ttcataaaaa
    14641 agctcctttc gttaattaat ctctcatgtt aatgtttcta ttattggaca cgaatgtaat
    14701 tgcttttcat ctggacatcg ttgttttaag tcgtctaaca tagtttcaat tcgttttaaa
    14761 tcctctattt gtttttgcac ttccttttgt tttttagaaa caaattcgaa catatcttga
    14821 caacgaactt catctttatc tacaacacca agtaatttat aaatctcgct taaagaaaaa
    14881 ccaagttcct gtattcgttt aataaaccca acacgcttaa cgtcatcata tgaatatatc
    14941 cgataaccag cttccgttcg gtgaggttct tgtaataaat tttttcgctc gtaatatctg
    15001 atcgtttctt tattaactcc acatttatct gcaaactcac taatgcgata aatcatctta
    15061 tttcacccca tgaatattat aaaccgtgta ccatagtaca cgttcaagta aattggacta
    15121 ttttattttt ttcccaaagt atcactcgta t
//
