LOCUS       NZ_LR135360            14158 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7948 plasmid 4.
ACCESSION   NZ_LR135360
VERSION     NZ_LR135360.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885488
            Assembly: GCF_900639585.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:54:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,768
            CDSs (with protein)               :: 2,768
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 53 of 121
            Pseudo Genes (incomplete)         :: 66 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 24 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135360.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7948"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            1..945
                     /locus_tag="EQB40_RS15460"
     CDS             1..945
                     /locus_tag="EQB40_RS15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1499
                     /locus_tag="EQB40_RS15465"
     CDS             966..1499
                     /locus_tag="EQB40_RS15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_077940945.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHEHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1514..1684
                     /locus_tag="EQB40_RS15805"
     CDS             1514..1684
                     /locus_tag="EQB40_RS15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            2094..3251
                     /locus_tag="EQB40_RS15470"
     CDS             2094..3251
                     /locus_tag="EQB40_RS15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_127820678.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVN
                     QGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            3261..3668
                     /locus_tag="EQB40_RS15475"
     CDS             3261..3668
                     /locus_tag="EQB40_RS15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232043233.1"
                     /translation="MLLVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHSERLVI
                     TNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQLDYTF
                     SLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            3969..4338
                     /locus_tag="EQB40_RS15480"
                     /pseudo=""
     CDS             3969..4338
                     /locus_tag="EQB40_RS15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4320..5271
                     /locus_tag="EQB40_RS16245"
                     /pseudo=""
     CDS             4320..5271
                     /locus_tag="EQB40_RS16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5511..6014
                     /locus_tag="EQB40_RS15495"
     CDS             5511..6014
                     /locus_tag="EQB40_RS15495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232043234.1"
                     /translation="MAFYLPYLLIFVSISGSIWLSCKIYQTRHSLKGSKIRFKRFLLLG
                     CIFSLIIISSGLFGVLEGNKRISGSILLENVTQKYEVARDKKKKEQALTQKIEKFTACY
                     EDMSDIFVKQEKRLTDKKIWKHSLNFIGICQKKTKKEYQEKYEQVKKKRRAICERYANG
                     RSVL"
                     /name="hypothetical protein"
     gene            complement(6341..6409)
                     /locus_tag="EQB40_RS15500"
     CDS             complement(6341..6409)
                     /locus_tag="EQB40_RS15500"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_331863273.1"
                     /translation="MPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            7072..8040
                     /locus_tag="EQB40_RS15505"
                     /pseudo=""
     CDS             7072..8040
                     /locus_tag="EQB40_RS15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            8061..8603
                     /locus_tag="EQB40_RS16250"
                     /pseudo=""
     CDS             8061..8603
                     /locus_tag="EQB40_RS16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            8618..8794
                     /locus_tag="EQB40_RS15810"
     CDS             8618..8794
                     /locus_tag="EQB40_RS15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164853350.1"
                     /translation="MTKKKHLLFGSILLGILSFSPMLLSVANKGNKKRGTKKLNPNYYN
                     SQNEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            9218..10102
                     /locus_tag="EQB40_RS15515"
     CDS             9218..10102
                     /locus_tag="EQB40_RS15515"
                     /inference="COORDINATES: protein motif:HMM:NF026081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="WP_232043235.1"
                     /translation="MKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQ
                     AINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNT
                     IKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLW
                     KSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWR
                     IAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIK
                     YANYCGVYPKKKK"
                     /name="cysteine protease staphopain"
     gene            10162..10368
                     /locus_tag="EQB40_RS16085"
     CDS             10162..10368
                     /locus_tag="EQB40_RS16085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033794861.1"
                     /translation="MGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISA
                     NAGPVTIPVPGGGFTWDNTITNW"
                     /name="hypothetical protein"
     gene            10341..10784
                     /locus_tag="EQB40_RS15520"
     CDS             10341..10784
                     /locus_tag="EQB40_RS15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            11086..11466
                     /locus_tag="EQB40_RS15525"
     CDS             11086..11466
                     /locus_tag="EQB40_RS15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            11448..12386
                     /locus_tag="EQB40_RS15530"
     CDS             11448..12386
                     /locus_tag="EQB40_RS15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_098379941.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDHVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNPKTRTYDQFCDSLKIFGIHCFNRGKNFTYELIKKKKKVRS
                     NKLGKDYEKETILHELDRREQTYYRERERAYHRQQADELRRARESIRKIAGEVRREQRT
                     LTSEQPEIRPTQRNHEQHIERGTDYQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            12626..13315
                     /locus_tag="EQB40_RS15540"
     CDS             12626..13315
                     /locus_tag="EQB40_RS15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065305728.1"
                     /translation="MAFYLPYLLIFVSISGSIWLSCKIYQTRHSLKGSKIRFKRFLLLG
                     CIFSLIIISSGLFGVLEGNKRISGSILLENVTQKYEVARDKKKKEQALTQKIEKFTACY
                     EDMSDIFVKQEKRLTDKNMETLTQLYRNLPEKQQKEYQEKYEQVKKDVQYVKDTQTEEA
                     CYDLFSDTIPFSTSEQERKERQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7073..8040
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4321..4682
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.215"
                     /coverage="100.0"
                     /evalue="1.32e-67"
     Typing          11449..12383
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.385"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca
     1141 atcaaaggtt gctagataag ttgcatgaac atgaacaaaa caacgataaa aatgcttata
     1201 taccaaacaa aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg
     1321 accagcaaca acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggaacaac
     1381 tgcagttgac ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa
     1441 cagaaacaat ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa
     1501 gaaggaaata cttatgacaa aaaaacactt gctattcggt tctatcctac taggaatatt
     1561 aagctttagc ccaatgttat tatcagtggc caacaaagga aataaaaaag gggaactaaa
     1621 actcaatcct aattactaca acagtcaaaa tgaggaagta tttgtagaaa aacttgataa
     1681 ataagaaaag actgctcgta cgagcagtct ttctgaaaga gattatccta ttattaacct
     1741 tagaaaagaa taataaatga atatgagata tgtaaaaaac ataacaaaca caaacaaaaa
     1801 taacaagtat ttaaaaaatg accgatcaat attcacaaag aattagaaag aaacaaccgc
     1861 agaaaaccaa gccttaaaaa gagcttttga cctagaccaa tcgaaaatca caaagtgagc
     1921 ccctgtatcc cttggtagag aggctacttg gcatactgta cattatgtta actaacgcta
     1981 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
     2041 catattgtat attttaatta taggtgtata ccacttaaat ttaacacaaa ggcgtgatta
     2101 ttttgaaacc caaaaagaaa atttatctag gtctcctctt ttccttgatg attggaattt
     2161 ttagtatagg aattacaaat acctatgctt cagaaaattc taatttcaat atcaactccc
     2221 aaccaattga agaacaagca attaattttg cgaaaactga ttttgaacac tccataactt
     2281 caattctttt aatggaaaat aacaccgaaa ctgatattaa ctatactctt ggttcgccat
     2341 ttaaaatgct cgatggagaa aatacggcaa atagttctat tttctttcca gtattttcag
     2401 acgctgataa tacgattaaa tatatttata ccgtcaaaaa aaatacaaat ggagaatatt
     2461 caggaagtat ttcacaattt ttagcaaaag aacttcaaaa tctagtagat agtaatataa
     2521 cagatacaac cttatttttt caagataata gcatttatta ttccgataat ggacaaatta
     2581 aactactttg gaaatcacct ttaagtacag aaaaaaatgg tgtttcttcc tttacagaaa
     2641 aaaatgatct tatatccgta aatataaaaa ataaaattaa aaatttctct aaaaaacaaa
     2701 attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg agataacttt attatgatta
     2761 attggcgtat agcagaaacc caaacaagtt ttccgtggtg tgctgcttac gtagaagctg
     2821 ctatcttatg taacaaaaca gatgtacgtg ctacttcagc tagagccatt atgcattata
     2881 cctatcctaa tttaaacaac agtcaaattg aatcaaaaag tcttaatcaa gatcaaataa
     2941 taaaatatgc aaattactgt ggtgtttatc ctaaaaaaaa agtagcacga atactaaacc
     3001 tttcagaggt ccaaacacaa ctaagaaaag gaaatgccat ttatatggga actgctggtt
     3061 atggaaatta tagtaacagc cgtcatgcac ttgcattatt tgggtgggtc aatcaaggtg
     3121 gttatcaaac ttactatgtg tggaatcctt ggtataacta cccaactgta ataagtgcaa
     3181 atgcaggacc agttacaatt cctgtacctg gaggtggttt tacttgggac aataccatta
     3241 caaattggta aataaaccca ttgcttttag ttatgggtct agtgttagtt cttttcttaa
     3301 ctagttgttc aaaaaaacaa gaaatacaaa agactgatct gattcctttt ctaggaactt
     3361 ggcaaactaa agatcattca gaacgtctag taatcactaa tttaaatact tcaaaaaata
     3421 tagaacaata taccatctta gtaaaagaga acaatcataa aattcaaatc attgataaaa
     3481 atttaaatat aaatgaacaa gttttcattt acaaagcaac aaataattta ctaacactgc
     3541 gctcgaataa aaaacaatta gattacactt ttagtttgga ttctccaaaa aagctcgtat
     3601 ttattttgac aacgaatgat gggacagaag ggacagctaa accaattgaa ttttttaaag
     3661 ctgattgatg aatagttgtt tgatattaca aaacaataat ttcaccgaag gtgaaacaag
     3721 cgaaacttta caaaaagtgg ctagcatttg ctagcctttt ttgtattgtg gagcgtgggt
     3781 tttgaggggg attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat
     3841 acgtaggtat ttggctagca aatatagagc ttgccaagcc ctttagtgtg ttttgtagaa
     3901 agggacctta tagtcacttt cacattacaa acgatacgtc cataggacgt atcaaaaggg
     3961 tgggacttat gagcaaatta aagcaaaatt tggctagcaa tccttctaaa aaatacacct
     4021 attgatcaga gtcaaaataa atcagttttc gagtgagcga atccaaattt gcaactctga
     4081 agctttgcaa atgagtgtgc cggcttccgt aaaagtctag gcacaaaacg cgcgtctggt
     4141 gacaccgaaa gtggcgccag acattgccca ggcaatcgcg cgcgatttag ccaaagctgg
     4201 agggaatatc aatcaaattg ccaagtggtg caatacacac caacacgacg ttgctcctgg
     4261 cgacgcccaa cgcttatccg aaaacctaaa aaaatcatgc aaaaggagct acaaaagata
     4321 tggcaacagt taaagtaagt cgaaccactt cctgtagccg agcaattaat tacgcagaac
     4381 cacgagcaac cgtcaaaaca ggaataaact gtgacattga ttatgccaaa agtgaaatga
     4441 aacaaatacg tatgctttac ggaaaagacg atcatgtaca agcacactta ttgattcagt
     4501 cctttcgacc aggagaaatt accgccgaaa aaaaagccaa ccagctagga aaagaatacg
     4561 ctgaaaaaat cgcaccagaa caccaaatcg cgatttatac gcacacggac aaagaccata
     4621 tccacaatca cattgtcatt aattcggtca atttagaaac aggaaaaaaa agtttcaagc
     4681 acatggtcaa gcattcctag ataagtgtta cgatatcaac gacgaaattt gtttgactca
     4741 tggtttaagt atcactgaac gaggaaaaaa aaaagaagaa aaacgaacta tgtctgaaat
     4801 caaactaaaa aaaaaaaatg aacctgtttg gaaagacgag attcgctttg ccattgatca
     4861 aaccatgaaa aatccaaaaa ccagaaccta tgaccaattt tgtgattccc tcaaaatatt
     4921 tgggattcat tgcttcaatc gtgggaaaaa aaattttact tacgaattaa tcaaaaaaaa
     4981 aaaaaaaaag tacgatccaa caaattagga aaggactatg aaaaggagac aattttacat
     5041 gagctggaca gacgagaaca aacctattat cgagaacgag aacgagccta tcatagacag
     5101 caagccgacg agcttcgaag agctcgagaa agcattcgga aaattgctgg agaagtacga
     5161 agagaacaac gaactcttac gtctgaacaa ccagaaatta gaccgactca aagaaatcat
     5221 gaacaacaca ttgaaagagg aacagactac caacaagaat tatcgctcta aaacgttacg
     5281 agacgtagaa aacgaaataa agaaacaacg tgcttttctt gctccctaca cacaagaaag
     5341 gatacaaact acgattgatt tacaagttaa aaaactccat caagagctat tagatcagcg
     5401 aaataaagaa caaaaaaagg cgctcattca acgatcaatg gattttcttg cccccttatg
     5461 ttgcttagtc ctgacactgt tcgcattttg gttagtatgg ggaggctaaa atggcatttt
     5521 atttacccta tctactcatt tttgttagta tttcaggaag tatttggctc agctgtaaaa
     5581 tttatcaaac aagacattca ttaaaaggct ctaaaattcg atttaagcgt tttttattgc
     5641 tcggatgtat attttcctta ataatcattt caagcggctt attcggcgtt ttagagggca
     5701 ataagcgcat ttcaggaagt attttattag aaaatgtcac tcaaaagtat gaagtagcac
     5761 gtgataaaaa gaaaaaagag caagcattaa cgcaaaaaat agaaaaattt accgcttgtt
     5821 atgaggacat gagtgatatt tttgtgaagc aagaaaagcg tttgacagat aaaaaaatat
     5881 ggaaacactc actcaacttt atcggaattt gccagaaaaa aacaaaaaaa gagtaccaag
     5941 aaaaatatga acaagtaaaa aaaaaaagac gtgcaatatg tgaaagatac gcaaacggaa
     6001 gaagcgtgtt atgacttatt cagtgataca atcccttttt ttcaactagc gaacaagaac
     6061 gaaaagaacg acaacaatca gtcacatatg aacgctatga aatcttgctt caacaagcaa
     6121 ctaacatgca aaatccgaca aaaaaaaaaa gaaaccgcct taaactatct aaaaaaaagt
     6181 gtcaaggaat ggttagatca gcaacagcaa aattgaattt gatcggcaat catgttaaac
     6241 taaacgtgca aatgatcaaa cattagatac atcgaaagcc ccagagaggt cggattctct
     6301 ggggcttttt tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat
     6361 tcaaccaata actgaataaa gagacacaaa cttctacaag aaggggcaaa tgaacaattc
     6421 aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca agttattata
     6481 tcttaaaaaa aattaaactt gtgtggtgtt tttttaagta tcgcttattt tagggttgtt
     6541 agggcttgcc ctgaccgcct gtaaggcgct ttaaaacaag ataagggatt ttatctagac
     6601 ttgtcttaaa acgccgtaga tgagcgctca gagcttctaa aaggactcct tacgaactgt
     6661 ttttaagcaa aggagccttt aggcgactgc gcagtttaat gtgagagcag agcgatcaca
     6721 cctttttagc gaaccgtagg gagcaaggtt tggaattggc agttttgata aattcgcgaa
     6781 cctgtttaga gaaaaaagga gtgatttttt aggcgggaaa attagcctcc cctctttttt
     6841 tatatctttt ttaaacctct ttttaaacct ctttaccgtc ggctgtgtcc ttactctccc
     6901 aagggataca gaatgttatg aaggacattt tgtctgttta tgaaggacat tttgtctgtt
     6961 tatgaaggac attttgtctg tttatgaagg acattttgtc tgtttgaacg tcaaatatga
     7021 atgtcttgac atttaaaaaa atgtcattca taattggatt gaggtgatta tatggctaat
     7081 gagattgtaa aaaaatacca ccatgaatta aatacaattc ctttaagaaa aaatttacac
     7141 cggttgaaat gaattttttt ttttccatcg tgtctagaat gcgtgaaaaa aaggggataa
     7201 gacagttcgt tttacgtttg accaactaaa agatttaagt aactacaaag caactgcaaa
     7261 tgtacgattt gtagaagatt taaaagcaac ttatgaaaaa aaaatcttat ctttacgctt
     7321 tggtcgtaga agtgagtctg ggctttctta cgatatgttt gtaatgttta cagaatttaa
     7381 aatcaacggg gacgtagaaa atccttacgt agatatacga atttacgaaa aaaagcactg
     7441 cctttgttaa ataatttaga cgagtgggta cgctattctt tacaacaatt caacgagcta
     7501 caaagtggct atgcaaagac tatgtttcgt ctgttaaagc aatacagaac caaaggtttc
     7561 gcatatttct ccaaagaaga ctttcttgaa ttattagata ttcctaaaag ttataagcaa
     7621 cctgatatag ataaacgagt tataaaacct atacgtcaag aactaactgc aatcttcaaa
     7681 ggactaacaa tcaaaaaaag aaatacggaa aaggacgggg aaaacctgtt attggctatc
     7741 aatttacgtt caaacctgag atcaaaaagc agacgacttt aacaaacatg caccaaccaa
     7801 agaagaccaa aataaagcat tggctttagc tgataaaaaa aaaaagaata cgaagaaccc
     7861 gctacaacaa ttaacgaaaa aaaaatcaaa cagaagaaac gagaacttga actcctccaa
     7921 caaatcaaac aactagaaaa aaagaaaaaa gctcttgaaa aaaaaaacaa acagagtccg
     7981 cactttcgga aaaaaagaaa aaaaagttta ttatctgatt tagataattt attccagtag
     8041 caacctagga ggcaaccagt atgcaaccac aagaaacctt tacaataaag gaattgtcag
     8101 atcttttttt aagatgagta ggcaagcaat atcgaaacat atccaaaaaa ttagattctt
     8161 ccatgatagc taaaaaatga gcgtggttat aaggttgtat tgcgttctga gtattgcaac
     8221 ttgctcgcaa cttagacaat caaaggttgc tagataagtt gcatgaacat gaacaaaaca
     8281 acgataaaaa aatgcttata taccaaaaaa aaacgtggtt gcaaacaagt tgctagatca
     8341 gttgcgtgaa gaaaatgaat ttttacgaga acaacttgcc ctcaaagagc aaaataattc
     8401 tcatatataa aaaactactc gaccagcaac aacaactcac cctgcaagcc aaccaacaaa
     8461 tcgaaaaact tcaggaacaa ctgcagttga cttatacgga agagtcttca gagaatagtt
     8521 caacgactct ttttctgaag caacagaaac aatggacgaa caaacaaaga attgaaaaaa
     8581 aatggtggca gctatggaaa tgaagaagga aatacttatg acaaaaaaaa aacacttgct
     8641 attcggttct atcctactag gaatattaag ctttagccca atgttattat cagtggccaa
     8701 caaaggaaat aaaaaaaggg gaactaaaaa actcaatcct aattactaca acagtcaaaa
     8761 tgaggaagta tttgtagaaa aacttgataa ataagaaaag actgctcata catgagcagt
     8821 ctttctgaaa gagattatcc tattattaac cttagaaaag aataataaat gaatatgaga
     8881 tatgtaaaaa aacataacaa acacaacaaa aaaataacaa gtatttaaaa aatgaccgat
     8941 caatattcac aaagaattag aaaagaaaca accgcagaaa accaagcctt aaaaagagct
     9001 tttgacctag accaatcgaa aatcacaaag tgagcccctg tatcccttgg tagagaggct
     9061 acttggcata ctgtacatta tgttaactaa cgctataaat aacgattatg ttcccaaaac
     9121 aatattttat gaactataac aaaaatttgt tatcgcatat tgtatatttt aattataggt
     9181 gtataccact taaatttaac acaaaggcgt gattattttg aaacccaaaa agaaaattta
     9241 tctaggtctc ctcttttcct tgatgattgg aatttttagt ataggaatta caaataccta
     9301 tgcttcagaa aattctaatt tcaatatcaa ctcccaacca attgaagaac aagcaattaa
     9361 ttttgcgaaa actgattttg aacactccat aacttcaatt cttttaatgg aaaataacac
     9421 cgaaactgat attaactata ctcttggttc gccatttaaa atgctcgatg gagaaaatac
     9481 ggcaaatagt tctattttct ttccagtatt ttcagacgct gataatacga ttaaatatat
     9541 ttataccgtc aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc
     9601 aaaagaactt caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga
     9661 taatagcatt tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag
     9721 tacagaaaaa aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat
     9781 aaaaaataaa attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa
     9841 aagtgtctcc tccggagata actttattat gattaattgg cgtatagcag aaacccaaac
     9901 aagttttccg tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt
     9961 acgtgctact tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca
    10021 aattgaatca aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt
    10081 ttatcctaaa aaaaaaaagt agcacgaata ctaaaccttt cagaggtcca aacacaacta
    10141 agaaaaggaa atgccattta tatgggaact gctggttatg gaaattatag taacagccgt
    10201 catgcacttg cattatttgg gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg
    10261 aatccttggt ataactaccc aactgtaata agtgcaaatg caggaccagt tacaattcct
    10321 gtacctggag gtggttttac ttgggacaat accattacaa attggtaaat aaacccattg
    10381 ctttagttat gggtctagtg ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa
    10441 tacaaaagac tgatctgatt ccttttctag gaacttggca aactaaagat cattcagaac
    10501 gtctagtaat cactaattta aatacttcaa aaaatataga acaatatacc atcttagtaa
    10561 aagagaacaa tcataaaatt caaatcattg ataaaaattt aaatataaat gaacaagttt
    10621 tcatttacaa agcaacaaat aatttactaa cactgcgctc taataaaaaa caattagatt
    10681 acacttttag tttggattct ccaaaaaagc tcgtatttat tttgacaacg aatgatggga
    10741 cagaagggac agctaaacca attgaatttt ttaaagctga ttgatgaata gttgtttgat
    10801 attacaaaac aataatttca ccgaaggtga aacaagcgaa actttacaaa aagtggctag
    10861 catttgctag ccttttttgt attgtggagc gtgggttttg agggggattc aagggggagc
    10921 ttgcgcccct ttgacaagcg attatttgca ccaaatacgt aggtatttgg ctagcaaata
    10981 tagagcttgc caaacccttt tagtgagctt tactcattgt acctttttag gtacaatgat
    11041 attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag cgaacaaaat
    11101 caaaatttgg ctagcgatca ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc
    11161 agttttcgag tgagcgaatc cgaatttgca aagctgaagc agtcagctga agctttgcaa
    11221 atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa
    11281 gtggcgccag acattgccca ggcaatcgcg cgcgatttag ccaaagctgg agggaatatc
    11341 aatcaaattg ccaagtggtg caatacacac caacacgacg ttgctcctgg cgacgcccaa
    11401 cgcttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg gcaacagtta
    11461 aagtaagtcg aaccacttcc tgtagccgag caattaatta cgcagaacca cgagcaaccg
    11521 tcaaaacagg aataaactgt gacattgatt atgccaaaag tgaaatgaaa caaatacgta
    11581 tgctttacgg aaaagacgat catgtacaag cacacttatt gattcagtcc tttcgaccag
    11641 gagaaattac cgccgaaaaa gccaaccagc taggaaaaga atacgctgaa aaaatcgcac
    11701 cagaacacca aatcgcgatt tatacgcaca cggacaaaga ccatatccac aatcacattg
    11761 tcattaattc ggtcaattta gaaacaggaa aaaagtttca agcacatggt caagcattcc
    11821 tagataagtg ttacgatatc aacgacgaaa tttgtttgac tcatggttta agtatcactg
    11881 aacgaggaaa aaaagaagaa aaacgaacta tgtctgaaat caaactaaaa gaaaaaaatg
    11941 aacctgtttg gaaagacgag attcgctttg ccattgatca aaccatgaaa aatccaaaaa
    12001 ccagaaccta tgaccaattt tgtgattccc tcaaaatatt tgggattcat tgcttcaatc
    12061 gtgggaaaaa ttttacttac gaattaatca aaaaaaagaa aaaagtacga tccaacaaat
    12121 taggaaagga ctatgaaaag gagacaattt tacatgagct ggacagacga gaacaaacct
    12181 attatcgaga acgagaacga gcctatcata gacagcaagc cgacgagctt cgaagagctc
    12241 gagaaagcat tcggaaaatt gctggagaag tacgaagaga acaacgaact cttacgtctg
    12301 aacaaccaga aattagaccg actcaaagaa atcatgaaca acacattgaa agaggaacag
    12361 actaccaaca agaattatcg ctctaaaacg ttacgagacg tagaaaacga aataaagaaa
    12421 caacgtgctt ttcttgctcc ctacacacaa gaaaggatac aaactacgat tgatttacaa
    12481 gttaaaaaac tccatcaaga gctattagat cagcgaaata aagaacaaaa aaaggcgctc
    12541 attcaacgat caatggattt tcttgccccc ttatgttgct tagtcctgac actgttcgca
    12601 ttttggttag tatggggagg ctaaaatggc attttattta ccctatctac tcatttttgt
    12661 tagtatttca ggaagtattt ggctcagctg taaaatttat caaacaagac attcattaaa
    12721 aggctctaaa attcgattta agcgtttttt attgctcgga tgtatatttt ccttaataat
    12781 catttcaagc ggcttattcg gcgttttaga gggcaataag cgcatttcag gaagtatttt
    12841 attagaaaat gtcactcaaa agtatgaagt agcacgtgat aaaaagaaaa aagagcaagc
    12901 attaacgcaa aaaatagaaa aatttaccgc ttgttatgag gacatgagtg atatttttgt
    12961 gaagcaagaa aagcgtttga cagataaaaa tatggaaaca ctcactcaac tttatcggaa
    13021 tttgccagaa aaacagcaaa aagagtacca agaaaaatat gaacaagtaa aaaaagacgt
    13081 gcaatatgtg aaagatacgc aaacggaaga agcgtgttat gacttattca gtgatacaat
    13141 ccctttttca actagcgaac aagaacgaaa agaacgacaa caatcagtca catatgaacg
    13201 ctatgaaatc ttgcttcaac aagcaactaa catgcaaaat ccgacaaaaa aagaaaccgc
    13261 cttaaactat ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa attgaatttg
    13321 atcggcaatc atgttaaact aaacgtgcaa atgatcaaac attagataca cgaaagcccc
    13381 agagaggtcg gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact
    13441 tcttgtttcg gttattcaac caataactga ataaagagac acaaacttct acaagaaggg
    13501 ggcaaatgaa caattcaaac attagataca cctccttcca gtcgcaagac tgtcggatag
    13561 gcgacaagtt attatatctt aaaaaattaa acttgtgtgg tgtttttaag tatcgcttat
    13621 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaagct agataaggat
    13681 ttttatccaa gactgtctta aaacggctta gataagcatt cagaaccgct caaaggcctc
    13741 tttaccaaca tgtttttagc aaaggagcct ttaggcgact gcgcagttca atgtgagagc
    13801 gtagcgatca cacctttttg gcgaccgtag gaagcaaggt tttggattgg cagttttgaa
    13861 aaatgcgcga acctgtttaa agaaaaaaga agtggttttt aggcgggaaa attagccccc
    13921 ctctttttat atctcttttt aaacctcttt ttaaacctct ttaccgtaag ctgtgtcctt
    13981 actctaccaa gggatacaga atgttatgaa ggacattttg tctgtttatg aaggacattt
    14041 tgtctgttta tgaaggacat tttgtctgtt tatgaaggac attttgtctg tttgaacgtc
    14101 aaatatgaat gtcttgacat ttaaaaaatg tcattcataa ttggattgag gtgattat
//
