LOCUS       NZ_LR135356             2856 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8014 plasmid 6.
ACCESSION   NZ_LR135356
VERSION     NZ_LR135356.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885529
            Assembly: GCF_900639575.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 82 of 138
            Pseudo Genes (internal stop)      :: 30 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135356.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8014"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            complement(309..536)
                     /locus_tag="EQB45_RS16470"
     CDS             complement(309..536)
                     /locus_tag="EQB45_RS16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729435.1"
                     /translation="MTEKFPYEYYQNLEKLESAIQLVKLDLLDENLLKYKNITPERKGK
                     LKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
                     /name="hypothetical protein"
     gene            complement(533..1507)
                     /locus_tag="EQB45_RS16475"
     CDS             complement(533..1507)
                     /locus_tag="EQB45_RS16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_010729436.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVGT
                     LNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQLRRG
                     KATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYRLRKLL
                     KQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2305..2586
                     /locus_tag="EQB45_RS16490"
     CDS             2305..2586
                     /locus_tag="EQB45_RS16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729433.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
                     /name="hypothetical protein"
     gene            2588..>2856
                     /locus_tag="EQB45_RS16495"
     CDS             2588..>2856
                     /locus_tag="EQB45_RS16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127821373.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGT"
                     /name="hypothetical protein"
     Typing          complement(573..1407)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.423"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 aaattctaaa gtgtatcaag aagtgaaaga aaaaaccgaa gctaaactaa atccgtcgaa
       61 tcgaaatcga ccaaagaatc atcaaaaaga aacgagtcaa acagtcactt ctagaaattt
      121 cgacccaaca aattttctaa aacgctaaaa aataaaacga gaattttttt atttctcgtt
      181 ttatttttta ggaaagccgg tcttacagca agatttgcaa gttaccggta acttgcggaa
      241 aagaagaagc cgctcacgcg acttcttctt tttgcatttt ctgaaagcta ccacaattcc
      301 taacttcgct aattatattc tccatgaaat tcgctccatg tatcaaaaat cacatctgaa
      361 attccatctt cttcatcaaa taattgctgc atttttttca atttaccctt tctctctggc
      421 gtaatatttt tgtattttag taaattctca tccaacaaat ccagcttgac caactgaata
      481 gcactctcca atttttctag attttgataa tactcgtatg gaaatttctc agtcattttt
      541 cttcctcctc tgtcacatct aaacctaaaa gccatgtatc caattctctt tttaattcgt
      601 ctgcagactc ctcaaatgtt tctcgtaaat gattcgtcaa ttcatcattt tgactttctt
      661 gcttcagcaa tttccttagc cgatatttca agtcacgact aatagttgac cactcagaat
      721 ggtcatacaa caaccccttt atcatcactc tatccaacgt ttttacatct tcaggaaaat
      781 aatgcggact agcaaaacta tccaaacttt cttgaaccat ctcataccaa tcagtcgctt
      841 ttccacggcg caattgtaac tccaaacgcc accacgtctc gacttcttta ggtacaattt
      901 ttcgctttgc ttcctgttcc aacttcttgt tgtacatccg aatttggcgt tcgctggcac
      961 gactacccca ataagccgtt tctagcttac cagagcgtcc ataaattggt ttgaaggaca
     1021 cagggtcaac tacacgatat tgtgtaataa actcatcagg cacatcaata agatcgcagg
     1081 caacatccgc tcgtgaaaaa tggggctcta aaaataaatc atgaataaat tttttcatag
     1141 agcttgctaa aaattgacta attttattgg ggttaaagtc aatccgccct ttaccctctt
     1201 gccatttcaa aatttcgata taggcaatat tttcttcgtg ttgatctacc aattgccaac
     1261 cattattccc aacagctttt aaatgaccat taccttcgtt cactcgcatt aattgctcaa
     1321 aatctaaaat caacacatca ttttgagtgt gataataaat attttcattc aaagttccga
     1381 caatcgtaat tcggtcaatc gaccaatcca tttttatcat ttttacgctc tcaccgacat
     1441 ttaaccgcct gttagagggg gctgttaatt tttcccactc attcacaatt gttctttttt
     1501 tacccaaatt ttgttcctcc tcaaagaaaa aaagagattt ttggaaagaa tttccagagt
     1561 tcacgtaact ctaaaaatcc acatccaaaa atctctagct tgaagaaacc taaaaatatt
     1621 cgtataatga acttgagtta cggaactagg ctagtagttt ccgtaaaggt cgagagtgtt
     1681 agcgcactgc tcgactattt ttttgctctt ttttctcgtt ttctttgatt atagcactcc
     1741 tcttaaaaaa ataaaaagac ctcagggaat ttcattcccc gaaccccttg agcctcctcc
     1801 tgccgaaatc atttcacgct tccgctcttc atttcattca gttctcggct ttcacaatct
     1861 aacacgccgt ttcgctacca gtcgccttac aggctccatt ctcgcttctc ttacgtgttt
     1921 taccgattgt gccgacaact aaacgaaatg aacgctaaaa cgtcttagaa cgcaaaatac
     1981 acgcttataa aatcatttca tcttgattat atgctcatca taatcgttca atttgacatc
     2041 atttccgtaa acttttttta tttttataag cagaaaaaag tcgcacatca ccattttata
     2101 aagagcataa ggaaacgtgt acccactttc taaaaaaagc gggcagatag gctgtgccta
     2161 tcacgtttct aagaaaaaaa tttgatatca ctttttcgcc attgtgacta gcttatgtat
     2221 agtgtttcag caaaaaacag ctatacaaat actatattcg tatgatatac ataaattata
     2281 cacaaatgcg aaaggagttt ttctatggac attttagttc ggaagattga ttcaaaggtt
     2341 attgcaaaaa tagacaaacg ttgccaagaa ctcacaaata aaactggcaa aaaatggagt
     2401 cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact tacgacttac
     2461 aaaaaagacc aattcgattt attactcgaa aaatttatcg ctattcagac aaaaaacact
     2521 caagtactaa acgactatgt tcatacaaat aatcaactta ttgaaacact tattggagga
     2581 aaatagcatg agaaaaactt ctgttcaaaa acacgttacc ctttatccca gtcacgtgga
     2641 cgttttagat tccattatcg aaagaaatcc agatatcaac aattactcgg aagccattcg
     2701 ttttttatgt atgagttatg aacctgatac caacacgact actaccagca atcaagccaa
     2761 actaaatgct atgggaaaag aaatttccat catgtccgaa atcattgcag aatttgctga
     2821 tttatatcta aaagatacag gcgttcttat tggcac
//
