LOCUS       NZ_LR135355             7038 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8014 plasmid 5.
ACCESSION   NZ_LR135355
VERSION     NZ_LR135355.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885529
            Assembly: GCF_900639575.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 82 of 138
            Pseudo Genes (internal stop)      :: 30 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8014"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            1..945
                     /locus_tag="EQB45_RS16415"
     CDS             1..945
                     /locus_tag="EQB45_RS16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1499
                     /locus_tag="EQB45_RS16420"
     CDS             966..1499
                     /locus_tag="EQB45_RS16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_077940945.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHEHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1514..1684
                     /locus_tag="EQB45_RS16425"
     CDS             1514..1684
                     /locus_tag="EQB45_RS16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            2094..3248
                     /locus_tag="EQB45_RS16430"
     CDS             2094..3248
                     /locus_tag="EQB45_RS16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            3221..3664
                     /locus_tag="EQB45_RS16435"
     CDS             3221..3664
                     /locus_tag="EQB45_RS16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            3966..4346
                     /locus_tag="EQB45_RS16440"
     CDS             3966..4346
                     /locus_tag="EQB45_RS16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4328..5266
                     /locus_tag="EQB45_RS16445"
     CDS             4328..5266
                     /locus_tag="EQB45_RS16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_098379941.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDHVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNPKTRTYDQFCDSLKIFGIHCFNRGKNFTYELIKKKKKVRS
                     NKLGKDYEKETILHELDRREQTYYRERERAYHRQQADELRRARESIRKIAGEVRREQRT
                     LTSEQPEIRPTQRNHEQHIERGTDYQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5506..6195
                     /locus_tag="EQB45_RS16455"
     CDS             5506..6195
                     /locus_tag="EQB45_RS16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065305728.1"
                     /translation="MAFYLPYLLIFVSISGSIWLSCKIYQTRHSLKGSKIRFKRFLLLG
                     CIFSLIIISSGLFGVLEGNKRISGSILLENVTQKYEVARDKKKKEQALTQKIEKFTACY
                     EDMSDIFVKQEKRLTDKNMETLTQLYRNLPEKQQKEYQEKYEQVKKDVQYVKDTQTEEA
                     CYDLFSDTIPFSTSEQERKERQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4329..5263
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.385"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca
     1141 atcaaaggtt gctagataag ttgcatgaac atgaacaaaa caacgataaa aatgcttata
     1201 taccaaacaa aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg
     1321 accagcaaca acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggaacaac
     1381 tgcagttgac ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa
     1441 cagaaacaat ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa
     1501 gaaggaaata cttatgacaa aaaaacactt gctattcggt tctatcctac taggaatatt
     1561 aagctttagc ccaatgttat tatcagtggc caacaaagga aataaaaaag gggaactaaa
     1621 actcaatcct aattactaca acagtcaaaa tgaggaagta tttgtagaaa aacttgataa
     1681 ataagaaaag actgctcgta cgagcagtct ttctgaaaga gattatccta ttattaacct
     1741 tagaaaagaa taataaatga atatgagata tgtaaaaaac ataacaaaca caaacaaaaa
     1801 taacaagtat ttaaaaaatg accgatcaat attcacaaag aattagaaag aaacaaccgc
     1861 agaaaaccaa gccttaaaaa gagcttttga cctagaccaa tcgaaaatca caaagtgagc
     1921 ccctgtatcc cttggtagag aggctacttg gcatactcga cattatgtta actaacgcta
     1981 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
     2041 catattgtat attttaatta taggtgtata ccacttaaat ttaacacaaa ggcgtgatta
     2101 ttttgaaacc caaaaagaaa atttatctag gtctcctctt ttccttgatg attggaattt
     2161 ttagtatagg aattacaaat acctatgctt cagaaaattc taatttcaat atcaactccc
     2221 aaccaattga agaacaagca attaattttg cgaaaactga ttttgaacac tccataactt
     2281 caattctttt aatggaaaat aacaccgaaa ctgatattaa ctatactctt ggttcgccat
     2341 ttaaaatgct cgatggagaa aatacggcaa atagttctat tttctttcca gtattttcag
     2401 acgctgataa tacgattaaa tatatttata ccgtcaaaaa aaatacaaat ggagaatatt
     2461 caggaagtat ttcacaattt ttagcaaaag aacttcaaaa tctagtagat agtaatataa
     2521 cagatacaac cttatttttt caagataata gcatttatta ttccgataat ggacaaatta
     2581 aactactttg gaaatcacct ttaagtacag aaaaaaatgg tgtttcttcc tttacagaaa
     2641 aaaatgatct tatatccgta aatataaaaa ataaaattaa aaatttctct aaaaaacaaa
     2701 attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg agataacttt attatgatta
     2761 attggcgtat agcagaaacc caaacaagtt ttccgtggtg tgctgcttac gtagaagctg
     2821 ctatcttatg taacaaaaca gatgtacgtg ctacttcagc tagagccatt atgcattata
     2881 cctatcctaa tttaaacaac agtcaaattg aatcaaaaag tcttaatcaa gatcaaataa
     2941 taaaatatgc aaattactgt ggtgtttatc ctaaaaaagt agcacgaata ctaaaccttt
     3001 cagaggtcca aacacaacta agaaaaggaa atgccattta tatgggaact gctggttatg
     3061 gaaattatag taacagccgt catgcacttg cattatttgg gtgggtcaat caaggtggtt
     3121 atcaaactta ctatgtgtgg aatccttggt ataactaccc aactgtaata agtgcaaatg
     3181 caggaccagt tacaattcct gtacctggag gtggttttac ttgggacaat accattacaa
     3241 attggtaaat aaacccattg ctttagttat gggtctagtg ttagttcttt tcttaactag
     3301 ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt ccttttctag gaacttggca
     3361 aactaaagat cattcagaac gtctagtaat cactaattta aatacttcaa aaaatataga
     3421 acaatatacc atcttagtaa aagagaacaa tcataaaatt caaatcattg ataaaaattt
     3481 aaatataaat gaacaagttt tcatttacaa agcaacaaat aatttactaa cactgcgctc
     3541 taataaaaaa caattagatt acacttttag tttggattct ccaaaaaagc tcgtatttat
     3601 tttgacaacg aatgatggga cagaagggac agctaaacca attgaatttt ttaaagctga
     3661 ttgatgaata gttgtttgat attacaaaac aataatttca ccgaaggtga aacaagcgaa
     3721 actttacaaa aagtggctag catttgctag ccttttttgt attgtggagc gtgggttttg
     3781 agggggattc aagggggagc ttgcgcccct ttgacaagcg attatttgca ccaaatacgt
     3841 aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt tactcattgt
     3901 acctttttag gtacaatgat attgcgaacg atacgtccgt aggacgtact gaaagggtgg
     3961 gacttatgag cgaacaaaat caaaatttgg ctagcgatca ttctaaaaaa tatacctatc
     4021 gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca aagctgaagc
     4081 agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg
     4141 cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg cgcgatttag
     4201 ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac caacacgacg
     4261 ttgctcctgg cgacgcccaa cgcttatccg aaaacctaaa aatcatgcaa aaggagctac
     4321 aaaagatatg gcaacagtta aagtaagtcg aaccacttcc tgtagccgag caattaatta
     4381 cgcagaacca cgagcaaccg tcaaaacagg aataaactgt gacattgatt atgccaaaag
     4441 tgaaatgaaa caaatacgta tgctttacgg aaaagacgat catgtacaag cacacttatt
     4501 gattcagtcc tttcgaccag gagaaattac cgccgaaaaa gccaaccagc taggaaaaga
     4561 atacgctgaa aaaatcgcac cagaacacca aatcgcgatt tatacgcaca cggacaaaga
     4621 ccatatccac aatcacattg tcattaattc ggtcaattta gaaacaggaa aaaagtttca
     4681 agcacatggt caagcattcc tagataagtg ttacgatatc aacgacgaaa tttgtttgac
     4741 tcatggttta agtatcactg aacgaggaaa aaaagaagaa aaacgaacta tgtctgaaat
     4801 caaactaaaa gaaaaaaatg aacctgtttg gaaagacgag attcgctttg ccattgatca
     4861 aaccatgaaa aatccaaaaa ccagaaccta tgaccaattt tgtgattccc tcaaaatatt
     4921 tgggattcat tgcttcaatc gtgggaaaaa ttttacttac gaattaatca aaaaaaagaa
     4981 aaaagtacga tccaacaaat taggaaagga ctatgaaaag gagacaattt tacatgagct
     5041 ggacagacga gaacaaacct attatcgaga acgagaacga gcctatcata gacagcaagc
     5101 cgacgagctt cgaagagctc gagaaagcat tcggaaaatt gctggagaag tacgaagaga
     5161 acaacgaact cttacgtctg aacaaccaga aattagaccg actcaaagaa atcatgaaca
     5221 acacattgaa agaggaacag actaccaaca agaattatcg ctctaaaacg ttacgagacg
     5281 tagaaaacga aataaagaaa caacgtgctt ttcttgctcc ctacacacaa gaaaggatac
     5341 aaactacgat tgatttacaa gttaaaaaac tccatcaaga gctattagat cagcgaaata
     5401 aagaacaaaa aaaggcgctc attcaacgat caatggattt tcttgccccc ttatgttgct
     5461 tagtcctgac actgttcgca ttttggttag tatggggagg ctaaaatggc attttattta
     5521 ccctatctac tcatttttgt tagtatttca ggaagtattt ggctcagctg taaaatttat
     5581 caaacaagac attcattaaa aggctctaaa attcgattta agcgtttttt attgctcgga
     5641 tgtatatttt ccttaataat catttcaagc ggcttattcg gcgttttaga gggcaataag
     5701 cgcatttcag gaagtatttt attagaaaat gtcactcaaa agtatgaagt agcacgtgat
     5761 aaaaagaaaa aagagcaagc attaacgcaa aaaatagaaa aatttaccgc ttgttatgag
     5821 gacatgagtg atatttttgt gaagcaagaa aagcgtttga cagataaaaa tatggaaaca
     5881 ctcactcaac tttatcggaa tttgccagaa aaacagcaaa aagagtacca agaaaaatat
     5941 gaacaagtaa aaaaagacgt gcaatatgtg aaagatacgc aaacggaaga agcgtgttat
     6001 gacttattca gtgatacaat ccctttttca actagcgaac aagaacgaaa agaacgacaa
     6061 caatcagtca catatgaacg ctatgaaatc ttgcttcaac aagcaactaa catgcaaaat
     6121 ccgacaaaaa aagaaaccgc cttaaactat ctaaaaagtg tcaaggaatg gttagatcag
     6181 caacagcaaa attgaatttg atcggcaatc atgttaaact aaacgtgcaa atgatcaaac
     6241 attagataca cgaaagcccc agagaggtcg gattctctgg ggcttttttt caggttgatg
     6301 tcgcctttat tgattttact tcttgtttcg gttattcaac caataactga ataaagagac
     6361 acaaacttct acaagaaggg ggcaaatgaa caattcaaac attagataca cctccttcca
     6421 gtcgcaagac tgtcggatag gcgacaagtt attatatctt aaaaaattaa acttgtgtgg
     6481 tgtttttaag tatcgcttat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     6541 ctttaaagct agataaggat ttttatccaa gactgtctta aaacggctta gataagcatt
     6601 cagaaccgct caaaggcctc tttaccaaca tgtttttagc aaaggagcct ttaggcgact
     6661 gcgcagttca atgtgagagc gtagcgatca cacctttttg gcgaccgtag gaagcaaggt
     6721 tttggattgg cagttttgaa aaatgcgcga acctgtttaa agaaaaaaga agtggttttt
     6781 aggcgggaaa attagccccc ctctttttat atctcttttt aaacctcttt ttaaacctct
     6841 ttaccgtaag ctgtgtcctt actctaccaa gggatacaga atgttatgaa ggacattttg
     6901 tctgtttatg aaggacattt tgtctgttta tgaaggacat tttgtctgtt tatgaaggac
     6961 attttgtctg tttgaacgtc aaatatgaat gtcttgacat ttaaaaaatg tcattcataa
     7021 ttggattgag gtgattat
//
