LOCUS       NZ_LR135354            11424 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E8014 plasmid 4.
ACCESSION   NZ_LR135354
VERSION     NZ_LR135354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885529
            Assembly: GCF_900639575.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 82 of 138
            Pseudo Genes (internal stop)      :: 30 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E8014"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            512..1150
                     /locus_tag="EQB45_RS16345"
     CDS             512..1150
                     /locus_tag="EQB45_RS16345"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_232043711.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGHFNS
                     FFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTLVLLQSFPKNLF"
                     /name="Abi family protein"
     gene            1187..1867
                     /locus_tag="EQB45_RS16350"
     CDS             1187..1867
                     /locus_tag="EQB45_RS16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_127821369.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCHT
                     TIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCAEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            <1922..2008
                     /locus_tag="EQB45_RS16355"
                     /pseudo=""
     CDS             <1922..2008
                     /locus_tag="EQB45_RS16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /name="DUF4180 domain-containing protein"
     gene            complement(2395..3780)
                     /locus_tag="EQB45_RS16365"
     CDS             complement(2395..3780)
                     /locus_tag="EQB45_RS16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="WP_127821372.1"
                     /translation="MNENKKYKVIKAVAENKKQKKRASVELNLSVRQINRLVKDYQTNG
                     KEAFSHKNRGGKQRHGVPDQVKQQVVTIYQSFRVKPNVRHYTEILKEDYDINYSDTTIR
                     KILYDAHILSPKSQRKTRKRINALIRLKTKENSKQSLNQMVQLAKEQLELPEKVHPSRP
                     RMKYKGELIQMDASSYNWFGDQITHLHLAIDDASGNIIGAYFDTQETLKGYYHVLHQIL
                     TKQGIPLAFLTDKRTVFEYKSKNRKTVEEDTFTQFGFACHQLGIEIKTSSIPQAKGRVE
                     RLNGTVQSRLPVELEMAGINSLEEANLFLKKWVTAFNRKFGHKAKESIYEKAPKLSEIN
                     LLLARVAHRQVDSGHHVRYKNNYYLPVEGSEDMYFTRKSKALVIEAFDGEIYLNIAEKI
                     FTTRILEEHEQYSKELDSIPEQKKERRKYIPPQSHPWKLESFKRYLRSIDRTIEEYEAS
                     KIA"
                     /name="ISNCY family transposase"
     gene            3965..4108
                     /locus_tag="EQB45_RS16675"
     CDS             3965..4108
                     /locus_tag="EQB45_RS16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003344326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164852610.1"
                     /translation="MITVFKMLFGKNAKIGNRLADKESFLSFCLLTNGPFSASPLVTIT
                     DR"
                     /name="hypothetical protein"
     gene            4225..5298
                     /gene="cfr(D)"
                     /locus_tag="EQB45_RS16370"
     ARG             4226..5295
                     /name="cfr(D)"
                     /gene="cfr(D)"
                     /gene_name="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(D)"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL/LINCOSAMIDE/OXAZOLIDINONE/PLEUROMUTIL
                     IN/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5382..>6476)
                     /gene="guaA"
                     /locus_tag="EQB45_RS16375"
                     /pseudo=""
     CDS             complement(5382..>6476)
                     /gene="guaA"
                     /locus_tag="EQB45_RS16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051303.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003922 - GMP synthase (glutamine-hydrolyzing) activity
                     [Evidence IEA]; GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /name="guaA"
     gene            6541..7221
                     /locus_tag="EQB45_RS16385"
     CDS             6541..7221
                     /locus_tag="EQB45_RS16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_127821369.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCHT
                     TIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCAEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            7324..7701
                     /locus_tag="EQB45_RS16390"
     CDS             7324..7701
                     /locus_tag="EQB45_RS16390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127821370.1"
                     /translation="MSCYDNPKYKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTII
                     KNDSKSYFPENNLKETQKLYLYGGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPS
                     LEKSNYGFPENWRNKLKIMIV"
                     /name="hypothetical protein"
     gene            complement(8203..8619)
                     /locus_tag="EQB45_RS16395"
     CDS             complement(8203..8619)
                     /locus_tag="EQB45_RS16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(8621..8902)
                     /locus_tag="EQB45_RS16400"
     CDS             complement(8621..8902)
                     /locus_tag="EQB45_RS16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353845.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIENDF
                     DHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(9278..9802)
                     /locus_tag="EQB45_RS16405"
     CDS             complement(9278..9802)
                     /locus_tag="EQB45_RS16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002305828.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(9819..10787)
                     /locus_tag="EQB45_RS16410"
     CDS             complement(9819..10787)
                     /locus_tag="EQB45_RS16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002332718.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIAYFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     Typing          complement(9824..10787)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.764"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaacgtctga aaacgcaaat aagagcgtat gaaacaaaac aataagcaac cgattcagca
       61 gaattccgga ggagccattt tcgtacgtta gcaaaaaagt gctgttgcta aaagctgctt
      121 gcggtgcgga gcgaacaagg tctcctagag cgagacgtgt acaaacaagc ttacaaattc
      181 gtgaaaggca ccggttttgc gccatttcca agcaaaaaaa ggtctatcga gcttgctcga
      241 tagaccgatc gcatttagcc ccccgcattc taacaggggg ggaataatgt gaaaaattat
      301 ggtagtaatg cttgatatat aggcatttct tttggttgca cacttaaaaa aaatcacgat
      361 tttgacaact aaatttttaa ataaaaaagc tttacagttt aattcaaatg tgttttaata
      421 tatctatcag tacttgccac gcctctgatt taatatcatg cgcattatgg tagggctttt
      481 tttttatatt tttttaagaa agaagattct tatgaagaca tcaaatagaa aatataaagt
      541 taaaacagtt aatcatagaa ttccacctaa aaaggcatta tgtgtaatta ataattccaa
      601 agtaattaat caagagttaa aaccatattt atccgagcaa gaaatgataa aaaaaataag
      661 taaagatatt tttttaacaa attccgatac aattttatta gaaaagtttt taaagaaaaa
      721 tagttatttt cgatttagta tttatgttaa attaatgaaa aatatagata aagttactat
      781 tctagatgtt attgaaacgt acaaattaga ttcattcatt cgtaatcaat tgcattattt
      841 tgtgaatcaa atagaaattt tttggaaaaa atctctttca gataatatgt gcgtttctta
      901 tgaagaaact agcatatttc cgaagaatca gtgttatttg gataaaaata tttattcaga
      961 tttgaaatgg gctgaagata taattggtca ttttaatagt tttttttatt caaatcaaag
     1021 tcccactttt aaacatcatc atataaaaaa aaatcattgt ttaccaatat gggctttatt
     1081 tgaagaaata acatttggta gtttaacaac attggttctg ttgcaaagtt ttccaaaaaa
     1141 tctattttag tgtaaaattg agaaaaaaga cagagaggac agagtaatga atcattttaa
     1201 aggcaaacaa ttcaaaaaag acgtcattat tgtcgctgtt ggttactacc tgcgttacaa
     1261 tctaagctat cgtgaagttc aggaattgtt atatgatcgt ggaataaatg tttgtcatac
     1321 tacgatttat cgttgggtgc aagagtacag caaagtcctc tattatcttt ggaagaagaa
     1381 aaatagacaa tccttctatt catggaaaat ggacgaaacc tatatcaaaa ttaagggacg
     1441 ttggcattat ctttatcgtg caattgatgc ggacggctta accttagata tctggttacg
     1501 aaagaaacgg gatacgcaag cagcctatgc ttttcttaag cggttagtga agcagtttga
     1561 tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact
     1621 aaaagaatac ggcttttatc aagggacaga acatcgtacc attaaatacc tgaataattt
     1681 gattgaacaa gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
     1741 tgcctctacc acgattaaag gcatggaagc cattcgagga ttatataaga aaacccgaaa
     1801 agaaggcact ctcttcgggt tttcggtctg tgctgaaatc aaggtattat tgggaatccc
     1861 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
     1921 cgattttatt tatgaatgta ataaaggaaa agacatatta tttttgccaa atgaaaaaca
     1981 agctattgaa aagctaagta ttgattagtt cttaaaatat taagtgatgt ttttatattg
     2041 taatttaagt attatttata atcaaataga taaataggaa tttaaatgga gaaatcatat
     2101 gggaaacaaa acagatgcca ttgagataat aacatatgca gaagaaaatg gaattaaaat
     2161 ttggatatat ggtggttgag gaatagatgt atggaaacat acaactgccg ttttcttaaa
     2221 gggtaaggaa ttgtaaaatt ccctacctac tcgaccaagt aaattttact aataaattat
     2281 gtcaatcgaa agttaaaatg tcccaaaatg aaagctcaca ttagcgcaat ctttactgcg
     2341 taaaacttgc actaatgtga ccaaaattgg tagaatatga gacaggtgac tattttaggc
     2401 tattttacta gcctcatatt cttcgatagt acggtcaata cttcgaaggt accttttgaa
     2461 agactcgagt ttccatggat gtgactgtgg aggaatatac ttgcgtcttt cttttttttg
     2521 ttctgggata gaatctaatt ctttggagta ttgttcatgc tcttctagta ttctcgtcgt
     2581 aaagattttt tctgctatat ttagatagat ttccccatca aacgcttcga ttactaatgc
     2641 tttagatttc ctcgtaaaat acatgtcctc acttccttcc acgggaagat agtaattatt
     2701 tttatatcta acatgatgtc cgctatctac ttgtcgatgt gccacacgtg ctaataataa
     2761 attgatttct gatagttttg gtgctttttc ataaatagat tcttttgctt tatgaccaaa
     2821 ttttctgttg aatgctgtca cccatttctt taaaaataaa ttagcttctt ccaatgaatt
     2881 gataccagcc atttctaatt cgactggaag tcgtgattga accgttccgt ttaatctttc
     2941 tacgcgtcct ttagcttgcg ggatagaaga ggtcttaatc tcgattccta attgatgaca
     3001 ggcaaagccg aattgagtaa acgtatcctc ttcaaccgtc ttcctatttt ttgacttgta
     3061 ttcaaaaaca gtccgcttgt cagttaaaaa agctaatgga ataccttgtt tcgtcaggat
     3121 ttgatgaaga acatgatagt agccttttaa tgtttcttgc gtatcgaaat aagcccctat
     3181 aatatttcca gaagcatcat ctatagcaag atgtaaatga gttatttgat caccaaacca
     3241 attataagag ctggcatcca tttggattaa ttctcctttg tatttcattc gaggtctact
     3301 aggatgtact ttttcaggta attctagttg ttctttggct agctgaacca tctggtttag
     3361 cgattgttta ctgttttctt ttgttttcaa tctaattaag gcgttgattc ttttacgtgt
     3421 ttttctttgt gattttggtg aaagaatatg agcatcgtac aaaatttttc taatcgttgt
     3481 atctgagtag ttgatatcat agtcctcttt taaaatttca gtgtagtgcc tcacgttagg
     3541 tttcactcga aaagattggt agatggtaac cacctgttgt ttgacttgat caggcacgcc
     3601 atgcctttgt ttacctcctc tgtttttatg tgaaaatgct tcctttccat tcgtttgata
     3661 gtctttaacg agtcgattga tttgcctcac tgataagttg agttccacac ttgctcgttt
     3721 cttttgtttt ttattttcag ctactgcttt tatgacttta tattttttat tttcgttcat
     3781 cgttaacata atccttttca tcgtgatagt ttatcatttt gggacatttt atgctttgac
     3841 ctacttaaga cattatcacg ttcgaatgat actatatcat aaaattaact tctcatacct
     3901 ttactttctt aatctttcat gttaaaatac tcaataatca attcaaatca gggaggtgtg
     3961 agtaatgata accgttttta aaatgttatt tggaaaaaat gcaaaaatcg gcaaccgttt
     4021 agcagacaaa gagagctttc tgtctttttg tctgctaact aacggtccct tcagtgcttc
     4081 acctcttgta actataacgg atcgttaatc ttagcgcctt atataataag aagcacggaa
     4141 tcttgtctgt gcttttttgt ttaccttaaa tgacagatca ctctcattac tcataaaaaa
     4201 agagaacgtg agagggttta taatatgtta caaaaacaat taactaaata tcaacaaatt
     4261 gaaaaagttt taaaagagtg tcaacaacca aactaccgaa tgaagcaaat tcttcattgt
     4321 atttttaaag agaaaaaaac agatttcaat gagatgtctg tattacctaa aaatttacga
     4381 gatactttaa caacagaaat aggaacatca cttctaacag ttgagcccct tattgaacag
     4441 aagtcgcaac aagtgaggaa agtactcttt aatctttctg gagataatcg cattgaaaca
     4501 gtcaacatga cctatcaagc tggctgggaa tctttttgta tttcatctca atgtggctgc
     4561 aatctaggtt gtcaattttg cgctactgga aaaattggct taagaaaaaa tttaacggct
     4621 gaggaaataa cggatcaagt tctctacttt cttttaaaag gacaccaaat tgatagtatt
     4681 tcttttatgg gtatgggaga accacttgct aaccctcatt tttttgaggc tttagatatt
     4741 tttatgaatc ctgatatgtt tcaactgagc cctaggcgtt tatctgtttc aacgattggt
     4801 gtgattccta aaataaaacg actaacagct gaatatccac aagttcattt aactttttct
     4861 cttcactcac cttttgatga agagagaagt cagttaatgc cgattaataa aagttttccc
     4921 cttttaaaag tgatggatac tttagatgaa catataaaag taacttcaaa aaaagtttac
     4981 attgcttata ttttactacc tgatgtgaac gattctttga ctcatgcagt tgctttagca
     5041 gatttacttc gctctcggta taaaaaagga aagctgtatc atgtcaatct catcaggtac
     5101 aatcctactt ttgatgcacc aagaacgttc aagcaagtag atgaaaagca agttaaactg
     5161 ttctatcaaa ccttaatatc taaaggcata aatgtcacta ttagaagtca gtttggaatt
     5221 gaaattgatg ctgcctgtgg ccaattatat ggaaattatg agattaaaaa caaaaaaaca
     5281 atccaagtaa atgactagat tgtttctcat ttaattaaat tagttggata actaaaaagt
     5341 accgaataat ccataattag attattcggt acttttttaa attattccca ctctactgtt
     5401 gcaggtggtt tactcgtaat gtcatacacg atacggttaa cgtgagccac ttcgtttacg
     5461 atacgaactg agattttttg taataagtcc caatcaatac gagcgaagtc agcagtcata
     5521 ccgtcaattg atgtaatcgc acggatacca acagtgtagt cataagtacg accgtctccc
     5581 ataacaccca ctgagcggat gcctggcaga actgtaaagt attgccagat gtcgcggtct
     5641 aagccagcct tagcaatttc ttcacgtaaa atcgcgtcac tttcgcggac gatttctaat
     5701 ttttcttctg taatttcacc aagaacacgg atacctaatc caggtcctgg gaatggttgg
     5761 cgccatacta atgattcagg cattccaagc tcaattccta aagcacgtac ttcatcttta
     5821 aataaagtgt ttaatggttc gattaattcg aactccatgt cttcaggtaa gccaccaacg
     5881 ttatgatgtg atttaataac ttcagctgta tcagtaccac tttcgataac gtcagtgtat
     5941 aaagtacctt gagctaagaa atcaatacca tctaatttag ctgcttcatc atcgaataaa
     6001 taaatgaatt cattacctat gattttacgt tttgtttctg gatcagaaac gccagctaat
     6061 ttgcctaaga aacgatcttt agcattcaca cggatgatat ttaagccgaa tttaccagct
     6121 aaactatcca ttacttgatc gccttcgtct ttacgtaata aaccgtggtc tacgaagata
     6181 caagttaatt gatcgccaat tgctttttgt aaaagaacac caacgacact tgaatccacg
     6241 ccaccagaaa gagctagcaa gacttttttg tcgccaactt gttcacgaat tttgttgatt
     6301 tcaacatcaa tgaagctttc cattgaccaa tcacctttag cgccacaaat atcaaaagca
     6361 aaatgacgta gaacgtcgtt accatgttca gagtgacgga cttctggatg gaattgaacg
     6421 gcataaaggc ctttttcttg gttttccatc gctgaaatag ggcagctggt tctgttgcaa
     6481 agttttccaa aaaatctatt ttagtgtaaa attgagaaaa aagacagaga ggacagagta
     6541 atgaatcatt ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc tgttggttac
     6601 tacctgcgtt acaatctaag ctatcgtgaa gttcaggaat tgttatatga tcgtggaata
     6661 aatgtttgtc atactacgat ttatcgttgg gtgcaagagt acagcaaagt cctctattat
     6721 ctttggaaga agaaaaatag acaatccttc tattcatgga aaatggacga aacctatatc
     6781 aaaattaagg gacgttggca ttatctttat cgtgcaattg atgcggacgg cttaacctta
     6841 gatatctggt tacgaaagaa acgggatacg caagcagcct atgcttttct taagcggtta
     6901 gtgaagcagt ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt
     6961 gcctttaaga aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa
     7021 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
     7081 cgaagtttac gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat
     7141 aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtgctga aatcaaggta
     7201 ttattgggaa tcccagctta aatcatagat accgtaaggg attttattct ttatttaaaa
     7261 ctttgcaaca gaaccacaac atttattaat caacttaata cagagtatta taataattgg
     7321 gtcatgagct gttatgataa tcctaaatac aaaaaagcta tacgaagttg ggcaaacgta
     7381 attagagctt atagaaataa gatagctcat cattcaagaa tatatgcatt taaagttact
     7441 gacgctccaa caatcataaa aaatgattcc aaaagttatt ttcctgaaaa taacttaaaa
     7501 gaaacccaaa agttatattt gtatggagga ttatacgtta taaaacatct gttaatatat
     7561 gaagatagca tgacacaaac tttatggaat aaatttctaa tagatttaga aaaaaagatc
     7621 gataaaatcc cttccttaga aaaatccaat tatggttttc cggaaaattg gagaaacaag
     7681 ttaaaaataa tgattgttta attgttattt aaattgttag tagacataat gtacatacta
     7741 ccaagtagcc cctggatcaa ccgatccagg ggaagacttt gtgattttca attggtctat
     7801 ctaaaacatc tttttaaaag cttgattctt cgctgttggc ttttttcgat tctttgtgaa
     7861 tattcagtga ttattttttt actacttgtt attttccgag ggatttgtta aaagtttaat
     7921 agcgtttctc agtaagggac gtcgtttttt cattttccta tgtatggact gctcgaacga
     7981 gcagcctttt tttattccag ggaacgtcct attctttgag tttccatgct attgaataac
     8041 cgggacttag ggattagggc agcgccccaa aatgaaaaaa gaaaaatccg cttctgcgaa
     8101 ttcttctttt ttttcgcaag ttggtaccaa cttgcaaatc ttgctggaag accgatccct
     8161 aaaaagaaat cgaggaattt ttttgttttt cggtttcttt ttttatcgtt ttagaaaatt
     8221 tgttgggtcg aaatttctag aagtgactgt ttgactggtt tctttttgat gattctttgg
     8281 tcgatttcga tttgatggat ttagtttagc ttcgattttt tctttcactt cttgatatac
     8341 tttagaattt gtgccaataa gaacgcctgt atcttttaga tataaatcag caaattctgc
     8401 gatgatttca gtcatgatgg aaatttcttt tcccatagca tttagttttg cttgattgct
     8461 ggtagtggtc gtgttggtat caggttcata actcatacat aaaaagcgaa tggcttccga
     8521 gtaattgttg atatctggat ttttttcgat gatggaatct aaaacgtcaa cgtgactggg
     8581 ataaagggta acgtgttttt gaacagaagt ttttctcatg ttatattcct ccgataagtg
     8641 tttcaatgag ttgattattt gtgtgaacat agtcatttag tatgttcgta ttttctgctt
     8701 gaatggcaat aaatttttcg aataataaat cgaattgatc ttttttgtaa ttcataagtt
     8761 cgtggtcaaa atcgttttcg attaaaattt ttaggtattg atttcgactc cattttttgc
     8821 ccgttttggt tgttagttcc tgacaacgtt tatctattct tgtaataatt ttgggattga
     8881 tatttcgaac taaaatgtcc atggaaattt tcctttcgta tcagtgtata atttatgtat
     8941 atcatacaaa tatagtattt gtatagctat ttttcgccaa aacactatac atagcctagc
     9001 cacaatggca aaaaagtgat agcaaatttt tttctgagaa acgtgatagg cacagcttat
     9061 ctagcacctt tcgtaagaaa gtgataccac gtttccttat gctctttcaa aattcagaaa
     9121 aatcagctgt aaaacgagtg cagtccgagt aagattcaac gaaaaaacgg ggattggggc
     9181 gccaccaaaa tatcgcaaca tgggcccatg ttgctctgct cgcaaaagaa aaaagaagaa
     9241 taaaaccagc aatttttatt aatttaaatt gctggtttta ttgccagaat tgccaccatt
     9301 ttttctccgt ctttgattgt ttttctatcg attctgtttc ttcagaaaga gtcgttgatt
     9361 tattttccga agattcttcc gtataagtca actgtagttg ttcctgcagt ttctcgattt
     9421 gttggttggc ttgcagggtg agttgttgtt gttggtcgag taatttttgc atttgtttta
     9481 tttgctcgtc tttttcttgt agttgcatat ctttttgatt taactgttgt tctaggagag
     9541 aaaggacttc taattcggga acttggttgg cgtttttgtg gttttcggtt ttagtttttt
     9601 cgccaactag ttgttgattt gagttttcgt tttcgttaaa aaacattgat tttatagcct
     9661 tttgtccggt ttcattaatc acaaacttgt ttccgatttt cgaaaaccaa agttttcgat
     9721 tttcgtcaga aacttttttg ttaattgctg ttttactaac tcctaattct tcagcaagtt
     9781 ctttgattgt tttttctgtc ataaaatcta aaaacccctc aatctttgaa taagttttga
     9841 agattgtcta ataagtcttt ttttagttct gaaggaatat cggaatttat ctctgatttt
     9901 tgtgcattat tttctttatt ttgggtattt tgttctcttt ctaacttttc taactgttgt
     9961 aaaagttcca attttcgctt ttgttctttg attttttctt gtaatgaagt aggtgctttt
    10021 tcagattgaa cttgttggtt agctagttct aatgctttgt tttgttcttc ttttgttggg
    10081 gcatgtttgt taaagtcgtc tgattttttt atctcaggtt taaatgtaaa ttgatagcca
    10141 atgacaggct ttccccgtcc ttttccgtat ctcttttgga ttgtcagacc tttgaagatt
    10201 gcggttagtt cttggcgaat aggctttata atttttttat ttatatcact ttctctataa
    10261 cttttaggga tatcaagtaa ttcatgaaag tcttcttttg aaaaataggc aattcctttg
    10321 gttctgtatt gttttaataa tctaaacatg gttttggaat aactgctttc taattcattg
    10381 aattgttgga gagaatatcg aacccattct tcaagattat taagtaaggg taaggctttc
    10441 tcatggactt gtatttcagc aaaaggaata tcagcttttc catcgatttt aaattgatta
    10501 aataaaacaa atcgttcacg ttgcaaaccg tcagcacttc ttctaccaaa acgtaaatcc
    10561 attaatttgt catacgttgt ttctaaatca tctatgaatc gaacatttgc agttgcttta
    10621 taattgctta aatcttttaa ttgttcgaat gtaaattgta cttttttatc gcctacgtca
    10681 cgcattctag atactataga gaagaataaa ttcatttcta ctgacgtaaa tttgcgtaaa
    10741 ggaatcgtat ttaattcatg gtggtatttc acaatttcat ttgccataaa atcctccttc
    10801 aagattaact acttaacaaa attataaaga gctttctaat aggggtcaag cccctaatta
    10861 aagaccccta aaaacagaca aaaagacccc caaaaacaga caaaaagacc cctaaaaaca
    10921 gacaaaaaga cccctaaaaa cagacaaaaa gacccccaaa agattctgca caccttggga
    10981 gagtaagcat acagccgacg gtaaagaggt ttaaaaagag gtttaaaaag agattataaa
    11041 agaggagagg ggcaaaattt cccgcctaaa aattttctgc ttttttagcc aaacaggttc
    11101 gcgaatttta ctaaactggg aaacttgctc cctctggtcg ctaaaaaggt gtgagggaaa
    11161 aacaccctca cattgtacct gcgcagtcgc ctaaaggctc ctttgctaaa aacgttgttt
    11221 aaagagattt ttttagaggc tctgcgcgct cgtttaagcc gttttaagac agtttagtat
    11281 ggataaccct tatcttgttc ttaaagcacc ttacaggcgg tcagggcaag ccctaacaac
    11341 ccttaaagag cataaaacca tcctaaaatt cgatttaatc tgtcttatct atgttcttgt
    11401 atattttata taccttgggt agta
//
