LOCUS       NZ_LR135338             2857 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7471 plasmid 8.
ACCESSION   NZ_LR135338
VERSION     NZ_LR135338.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885231
            Assembly: GCF_900639495.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,269
            CDSs (total)                      :: 3,181
            Genes (coding)                    :: 3,071
            CDSs (with protein)               :: 3,071
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 45 of 110
            Pseudo Genes (incomplete)         :: 62 of 110
            Pseudo Genes (internal stop)      :: 25 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135338.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7471"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            24..305
                     /locus_tag="EQB74_RS17050"
     CDS             24..305
                     /locus_tag="EQB74_RS17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729433.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
                     /name="hypothetical protein"
     gene            307..723
                     /locus_tag="EQB74_RS17055"
     CDS             307..723
                     /locus_tag="EQB74_RS17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729434.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKTEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(884..1111)
                     /locus_tag="EQB74_RS17060"
     CDS             complement(884..1111)
                     /locus_tag="EQB74_RS17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729435.1"
                     /translation="MTEKFPYEYYQNLEKLESAIQLVKLDLLDENLLKYKNITPERKGK
                     LKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
                     /name="hypothetical protein"
     gene            complement(1108..2082)
                     /locus_tag="EQB74_RS17065"
     CDS             complement(1108..2082)
                     /locus_tag="EQB74_RS17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_010729436.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVGT
                     LNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQLRRG
                     KATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYRLRKLL
                     KQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(1148..1982)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.423"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 acaaatgcga aaggagtttt tctatggaca ttttagttcg gaagattgat tcaaaggtta
       61 ttgcaaaaat agacaaacgt tgccaagaac tcacaaataa aactggcaaa aaatggagtc
      121 gaaataaata tctaaaagtt ttaatcgaaa gtgattttga ccgtcaactt acgacttaca
      181 aaaaagacca attcgattta ttactcgaaa aatttatcgc tattcagaca aaaaacactc
      241 aagtactaaa cgactatgtt catacaaata atcaacttat tgaaacactt attggaggaa
      301 aatagcatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag tcacgtggac
      361 gttttagatt ccattatcga aagaaatcca gatatcaaca attactcgga agccattcgt
      421 tttttatgta tgagttatga acctgatacc aacacgacta ctaccagcaa tcaagccaaa
      481 ctaaatgcta tgggaaaaga aatttccatc atgtccgaaa tcattgcaga atttgctgat
      541 ttatatctaa aagatacagg cgttcttatt ggcacaaatt ctaaagtgta tcaagaagtg
      601 aaagaaaaaa ccgaagctaa actaaatccg tcgaatcgaa atcgaccaaa gaatcatcaa
      661 aaagaaacga gtcaaacagt cacttctaga aatttcgacc caacaaattt tctaaaacgc
      721 taaaaaataa aacgagaatt tttttatttc tcgttttatt ttttaggaaa gccggtctta
      781 cagcaagatt tgcaagttac cggtaacttg cggaaaagaa gaagccgctc acgcgacttc
      841 ttctttttgc attttctgaa agctaccaca attcctaact tcgctaatta tattctccat
      901 gaaattcgct ccatgtatca aaaatcacat ctgaaattcc atcttcttca tcaaataatt
      961 gctgcatttt tttcaattta ccctttctct ctggcgtaat atttttgtat tttagtaaat
     1021 tctcatccaa caaatccagc ttgaccaact gaatagcact ctccaatttt tctagatttt
     1081 gataatactc gtatggaaat ttctcagtca tttttcttcc tcctctgtca catctaaacc
     1141 taaaagccat gtatccaatt ctctttttaa ttcgtctgca gactcctcaa atgtttctcg
     1201 taaatgattc gtcaattcat cattttgact ttcttgcttc agcaatttcc ttagccgata
     1261 tttcaagtca cgactaatag ttgaccactc agaatggtca tacaacaacc cctttatcat
     1321 cactctatcc aacgttttta catcttcagg aaaataatgc ggactagcaa aactatccaa
     1381 actttcttga accatctcat accaatcagt cgcttttcca cggcgcaatt gtaactccaa
     1441 acgccaccac gtctcgactt ctttaggtac aatttttcgc tttgcttcct gttccaactt
     1501 cttgttgtac atccgaattt ggcgttcgct ggcacgacta ccccaataag ccgtttctag
     1561 cttaccagag cgtccataaa ttggtttgaa ggacacaggg tcaactacac gatattgtgt
     1621 aataaactca tcaggcacat caataagatc gcaggcaaca tccgctcgtg aaaaatgggg
     1681 ctctaaaaat aaatcatgaa taaatttttt catagagctt gctaaaaatt gactaatttt
     1741 attggggtta aagtcaatcc gccctttacc ctcttgccat ttcaaaattt cgatataggc
     1801 aatattttct tcgtgttgat ctaccaattg ccaaccatta ttcccaacag cttttaaatg
     1861 accattacct tcgttcactc gcattaattg ctcaaaatct aaaatcaaca catcattttg
     1921 agtgtgataa taaatatttt cattcaaagt tccgacaatc gtaattcggt caatcgacca
     1981 atccattttt atcattttta cgctctcacc gacatttaac cgcctgttag agggggctgt
     2041 taatttttcc cactcattca caattgttct ttttttaccc aaattttgtt cctcctcaaa
     2101 gaaaaaaaga gatttttgga aagaatttcc agagttcacg taactctaaa aatccacatc
     2161 caaaaatctc tagcttgaag aaacctaaaa atattcgtat aatgaacttg agttacggaa
     2221 ctaggctagt agtttccgta aaggtcgaga gtgttagcgc actgctcgac tatttttttg
     2281 ctcttttttc tcgttttctt tgattatagc actcctctta aaaaaataaa aagacctcag
     2341 ggaatttcat tccccgaacc ccttgagcct cctcctgccg aaatcatttc acgcttccgc
     2401 tcttcatttc attcagttct cggctttcac aatctaacac gccgtttcgc taccagtcgc
     2461 cttacaggct ccattctcgc ttctcttacg tgttttaccg attgtgccga caactaaacg
     2521 aaatgaacgc taaaacgtct tagaacgcaa aatacacgct tataaaatca tttcatcttg
     2581 attatatgct catcataatc gttcaatttg acatcatttc cgtaaacttt ttttattttt
     2641 ataagcagaa aaaagtcgca catcaacatt tttttaaaga gcataaggaa acgtgtaccc
     2701 actttctaaa aaaagcgggc agataggctg tgcctatcac gtttctaaga aaaaaatttg
     2761 atatcacttt ttcgccattg tgactagctt atgtatagtg tttcagcaaa aaacagctat
     2821 acaaatacta tattcgtatg atatacataa attatac
//
