LOCUS       NZ_LR135337             4374 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7471 plasmid 7.
ACCESSION   NZ_LR135337
VERSION     NZ_LR135337.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885231
            Assembly: GCF_900639495.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,269
            CDSs (total)                      :: 3,181
            Genes (coding)                    :: 3,071
            CDSs (with protein)               :: 3,071
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 45 of 110
            Pseudo Genes (incomplete)         :: 62 of 110
            Pseudo Genes (internal stop)      :: 25 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135337.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7471"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            complement(109..471)
                     /locus_tag="EQB74_RS17020"
     CDS             complement(109..471)
                     /locus_tag="EQB74_RS17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(475..783)
                     /locus_tag="EQB74_RS17025"
     CDS             complement(475..783)
                     /locus_tag="EQB74_RS17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(792..1211)
                     /locus_tag="EQB74_RS17030"
     CDS             complement(792..1211)
                     /locus_tag="EQB74_RS17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(1204..1437)
                     /locus_tag="EQB74_RS17035"
     CDS             complement(1204..1437)
                     /locus_tag="EQB74_RS17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(1440..2408)
                     /locus_tag="EQB74_RS17040"
     CDS             complement(1440..2408)
                     /locus_tag="EQB74_RS17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2855..4261)
                     /locus_tag="EQB74_RS17045"
     CDS             complement(2855..4261)
                     /locus_tag="EQB74_RS17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002317172.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     Typing          complement(1448..2325)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.973"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(2859..4261)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctctccgagg gtattcgctg tcacaagtga caacgaaaaa aggagccgaa agcgtgtcac
       61 gctctcaact ccttttacgc tttattttgc ttcacgcaaa ataatcgctt atcgtaaatt
      121 cacgtcatta aatattgacg taaatttact ctcactcatc tgacttttct tattcggtat
      181 cactccgaat aagccgtcag attcatcggg ataatctcgt tcgcttggcg ctgatttttc
      241 atcagcaagc cactcattat ccactgattt ttcgtgttgc tgatcgtcag aaatcgacga
      301 tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa cgcttgcgaa gagccttgcc
      361 cattgcagca ttttctttct caatcaagcg ttttttctgt tggcgaagtt tcgcttgttc
      421 tttttttaat ttttcttctt gcgctttaat atctgctaaa gtcattccca taaactacat
      481 ctccttttct ttggaattaa taacggaaat ttggtttaac tgcataacat gactcattaa
      541 tttcaaccaa aattcttttt ctgattcatc agttgtttgc gctaaatgct tatgaacaaa
      601 ttcattagtc aaaactgaag attttgctag atcagcattg ataaccgagt tgagaacatc
      661 catcattcgt tcaagcattt cactataatt atcttcatcc ggcaaattca aataatcagc
      721 taaccgttca tacatcaatt caattctatc gactgtttct tctctcaaat caattttatc
      781 catgtcacac ctcacttcca gaattgccac cattttcgat tttttaattc ttgattttct
      841 agtaacaact gatcgttttt ttgttgctga agcaaagtca atttttgttg gtcacgaatc
      901 atttccgtta attcagaaat atgcctatct ttttccgcta attgttcacg caaaaattca
      961 ttttcttttt ctaacgggaa atcattgcct gcatcattga ccggtaaatc accagtcaat
     1021 tgaccacctt ttgaccgata aattgaccgc acaacaccaa tcaattcctt gtctaacatg
     1081 ataaccccat tatctttata ccgagcaagg tcataacctg cattgaccag ttgattgcgc
     1141 actgtatttt tagatatacc caactcatca gcaaggtttt ttatggtcat tttttctggc
     1201 aaatcagtca ttatcttctc cgttactttc agctacccca taaacatatt taacagcatg
     1261 cttagggata atgattgggg aatcaatttt attatttttc aaaatcaact catactgcgt
     1321 tacatcaagt aacttaccgg acattttttt gctatccaaa aaaacaacga caacgttatc
     1381 acctgttaaa ttcaccaacc aatttttctt accaaaatgc ttttgttttt ctgccattct
     1441 tattcctcct ccgttacatc taacccttga agccaagtgt ctaattcttt tttcaaaaca
     1501 tctgctgatt ccgaaaatgt ttctcgtaaa tgattcgtca actcatcatt ttgactttct
     1561 cttttcaaaa tatctctcaa tttgtatttc attctacgct ctaaaaaccc ccacatttca
     1621 gggttagcaa tcagtccaaa aatcatcacc ttatcatttg tttttacatc aagtggtaga
     1681 aaatgagggc tagcaaaact atttaaactt tcatgaacca ttgcatgcca atctgttgct
     1741 ttcccacgcc ttaactgcaa ttccagtcgc caccaagttt ctatttctgg tggcacaatt
     1801 ttacgcttgg tttcctgttc caattttttg ttgtatagcc gaatctggcg ttcgctggca
     1861 cgactgcccc aataagctgt ttccagcttc ccagaccgcc cataaattgg cttgaaagaa
     1921 acaggatcaa ccactcgata ttgtgtaata aactcatcag gcacatcgat aatatcgcaa
     1981 gcaatatccg cacgtgagaa atgtggttct agaaataaat catgaatgaa atttttcatt
     2041 gaactagcta aaaaatgacc aatcttattc gggttaaaat caatccgccc tttgccatct
     2101 tgccatttca aaatctcaat ataagcaata ttttcttcgt attggtctaa taattgccag
     2161 ccgttattcc caaccgcttt taaatagcca ttgccttcat tcacccgcat taattgctca
     2221 aaatctaaaa tcaacacatc attttgagtg tgataataaa tattttcctt cagttttcca
     2281 acgatagtaa ttcggtcaat tgaccactgc atttttaagg gggtgtacat attttgtaca
     2341 tttaaccgcc tgttagatag ggctgttaaa ttttcccact catttacgat tatgccattt
     2401 ttactcaaat tttgttcctc ctcaaagaaa aagagacttc tggaaagaat tttcaaaagt
     2461 tcacgtaact cttaaaaatt cattaccaaa aaatctctag tcttgaagaa atctaaaaat
     2521 attcgtataa tgattaagaa gttacggagc tagtgcgatt agtttccgta aaggtcgaga
     2581 acgttggtag cgttgctcga ctattttttt actctttttt cagttcattt gtttgctaac
     2641 tcaataatag cacttctctt tagctatgca aatatccaaa aaataaaacc gcaccctttg
     2701 aaaagggcgc ccccaaactt cgctaacgct aggttggcca aaaaatggac tggccatttt
     2761 ttagcaaatg tcagcttaat aggctagtgg tttagccgtc aactccttat cctcggcaca
     2821 aaccgcctcc ggtaccgttg actgcacatc gtaactacca ctcgatctca tcttcagaac
     2881 gctctaattt acgctgtgag acgttttgac gataaaataa ggttttctcc tgctctaaca
     2941 cttctaattc ctcagaaagc cccatatcct tttctgcaag accaaaacgg atagacaaac
     3001 gagtgatcga tttttgaaat gcgtttttta atcgctctaa acgctggcgt aaagagcgat
     3061 tttcttcttt cagcttttta ttttccactt gtaaatctgc taattgtttt ctaccagaca
     3121 aatgacgctc ttcttccaat gtagctacat actcatttaa ttctgtattt tgctcaagca
     3181 tctgtaaata ttgctcactc atgttatcag catttctctt ggcatttgtt gctctcgtag
     3241 ctcgttgata caaatcaaca aaatcattcc cagataaatg atacgaacca tcaggaagcc
     3301 tcttgaaact ttctaaagga accatatctt tttggaaaat gaccgccatc tcttttttta
     3361 cattcagcat atttttcttc tcttcttcaa tcttcgctct ctcagaagcc atcagagcct
     3421 tatcacgctc gttttcttct ctttgcctag aaatatacct caactcatca gaaacgctct
     3481 cacgctcttt tacagccgtt tggcgcaatt cagaaatttc actcatgatt tctttgtatt
     3541 cgtgaatatc tttaattttg ttggtaccga caatatccct tttccaaccg taatcattca
     3601 ttaatcgttc tatagagtca acttcttgat ttctaaaatt attgaaaagc actcgaccat
     3661 tatctttatc agcgtgtatc ccttgttgaa ccagtgcttt attaaagctc ggctgctttt
     3721 gaacaccccg tttataacct tcagcaaccg gcacaatatt caagtgcagg tgcggactta
     3781 tttcgtcatt atgaataaca gcattataaa cagccaaatt agggtttctc tcttgaaact
     3841 ccttgtaata ctgaaccaaa attttattgg caatcactcg attttgttca gcttgttgtt
     3901 tcgatatgcc agtagaagaa ccatctgctc gatttgtctg gaaatcatct aaaatgccta
     3961 tttgcacaat aaattctcgt tgaggttcca actttttatc cttgcgcact ttcgctaaat
     4021 agtcctcgat ttttctatcg gctctttttt gtttcgcatt atatttttct accacttcgc
     4081 caaacaattc ctcatattta tctgctagat tcgtctgctc taaataaata ttttcatccg
     4141 atttcgtgaa gtcaatatgc ttatgccaat cattggtttt ctggcgatca gttaactcac
     4201 gattattatg agaaatactt gtattcttcg ttcctttttt aaatgaaata ctaaacccca
     4261 agttaaaacc ctccttcgcg ctgtcgctaa taaaaagatt gtaaaccttt ctgttactct
     4321 ttgtcaaaga gtaacatttt cctaattgtg acatggctgt cacaattcca aatg
//
