LOCUS       NZ_LR135336             9529 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7471 plasmid 6.
ACCESSION   NZ_LR135336
VERSION     NZ_LR135336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885231
            Assembly: GCF_900639495.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,269
            CDSs (total)                      :: 3,181
            Genes (coding)                    :: 3,071
            CDSs (with protein)               :: 3,071
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 45 of 110
            Pseudo Genes (incomplete)         :: 62 of 110
            Pseudo Genes (internal stop)      :: 25 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7471"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            1..933
                     /locus_tag="EQB74_RS16960"
     CDS             1..933
                     /locus_tag="EQB74_RS16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="EQB74_RS16965"
     CDS             950..1420
                     /locus_tag="EQB74_RS16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1971
                     /locus_tag="EQB74_RS16970"
     CDS             1504..1971
                     /locus_tag="EQB74_RS16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_010729430.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2098..2262
                     /locus_tag="EQB74_RS17300"
     CDS             2098..2262
                     /locus_tag="EQB74_RS17300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002350941.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELSELLTT"
                     /name="hypothetical protein"
     gene            complement(2403..3551)
                     /gene="tnpB"
                     /locus_tag="EQB74_RS16975"
     CDS             complement(2403..3551)
                     /gene="tnpB"
                     /locus_tag="EQB74_RS16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(3568..3972)
                     /gene="tnpA"
                     /locus_tag="EQB74_RS16980"
     CDS             complement(3568..3972)
                     /gene="tnpA"
                     /locus_tag="EQB74_RS16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            4601..5755
                     /locus_tag="EQB74_RS16985"
     CDS             4601..5755
                     /locus_tag="EQB74_RS16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_002348708.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            5728..6171
                     /locus_tag="EQB74_RS16990"
     CDS             5728..6171
                     /locus_tag="EQB74_RS16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            6473..6853
                     /locus_tag="EQB74_RS16995"
     CDS             6473..6853
                     /locus_tag="EQB74_RS16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002348702.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6835..7749
                     /locus_tag="EQB74_RS17000"
     CDS             6835..7749
                     /locus_tag="EQB74_RS17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7709..7987
                     /locus_tag="EQB74_RS17675"
     CDS             7709..7987
                     /locus_tag="EQB74_RS17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            7989..8678
                     /locus_tag="EQB74_RS17010"
     CDS             7989..8678
                     /locus_tag="EQB74_RS17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6836..7746
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatacat taataattaa tactttaatt attatcttta ataaacatag aaacttattt
     1921 cttcacagca tatttccaat tgttttatta atttttctat actttatata aaaagataga
     1981 actatttagt aagataaaaa agtagctaat tgtaattttc gtctaaaact caaaactagt
     2041 aaaaataact ttattgtgtt atttttattt ataaattatg aaaaaggacc tttcgaaatg
     2101 aaaaataata gaatctacaa aaatcagttg aattatgggc ttttattgcc agtttttctg
     2161 ctatgtatca ttggattact ttctttatat gttgctttat atcacgactc ttacactaat
     2221 tcaaaaatca caaaagaatt aagtgaacta ctcaccactt agctaaacct gacggttctt
     2281 aacgcttgaa gtgggagctt cttgggaata gagcatactt gatagcgtat ttctttacta
     2341 acagcggtgt ctcttattta ccaagctatc cccgtagttc ctacggttct tgatgattgg
     2401 atctaagcca atcccataga atgtagtctt agaccttcgg tcagaatatt gatactagca
     2461 ttgatatcac gatcatgatg agtatgacaa acaggacaag tccattctcg aatttcaaga
     2521 gatttcttgc catctttatg tccgcactct gaacaaatct gactagacgg aaaccattta
     2581 tctactttga tgatttttcg tccataccag tcagccttgt attgtaattt agtcacaaac
     2641 tttgaccaag aaacatcaga aatactttta gctaatttat gatttcgcaa catacctttt
     2701 gtgtttaagt cttcaataca gataatatcg tgatttttga tcatttctgt actcaactta
     2761 ttcagaaaat cagtacgttg attcattact ttttcatgca atctagctac tttgcgtttt
     2821 tgtttttgat aatttttagc ttcaaataga ttggtacctt tatttttagc caataaagca
     2881 cgcctagaca atttgcgttg ttcacgcttt agtttctttt ccattttgga cgtgaattta
     2941 ttattatcaa ttttttgtcc atcagaaaga atcgcaaagt ccataatacc taagtcaata
     3001 ccaattgtgg aattagtttt aggtaattcg ataatttctt ctttacacaa caaagaaata
     3061 tagtatttac cgctagaatg acgtgatatt gtagcggatt tgataatccc ttttggttgt
     3121 ctatgaagct taattcttac taatgacttc aatttaggaa ctttgatgaa tttactatca
     3181 atcaaagcaa ctgtaccatt ttgattattc gttgtataac tctgaacagg atttttctta
     3241 cttttgaaac gtggaaatcc aacggattta tcccgaaaaa aattcttgta tgctttatct
     3301 aaattaagtt gggcattggc taaagcaagg ctatcaattt ctttcaaaaa cggaaattct
     3361 ttcttgtatt tagctggtgt cggaaaagtc atttttttag aagaatcatt tttaacttct
     3421 tcatacgcct tttttcggtc atcaagcatt agattgtaga ccttacggac acaaccaaaa
     3481 gatttggcaa agaagatttc ttgttcctct gttggataga ttctgaattt gtatgctttt
     3541 agttgttcca taaagttcac ctactttcta tttatgattg tttccttgat tttggatata
     3601 cttcttaatc acatcaattg gtgcgccacc agttgtcaga agacaaaaac ttttagacca
     3661 gaacatttct ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga
     3721 gctggcactt ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa
     3781 cataatatga acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc
     3841 tgaaattctt tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct
     3901 acgatatttc gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt
     3961 atctaattcc atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa
     4021 ataaaaaggc aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc
     4081 gaacggcatt catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatt
     4141 caaattatta caacagtcct aacgaagaat tgtttgtaaa acaaacggat aaataataaa
     4201 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     4261 acgcgcttat gaagcgctac caaaaattta tcatatttta tgaaaaataa caccccagta
     4321 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caactgcaga aaatcaagcc
     4381 ttaaaaagag cttttgacct agaccaatga aaaatcacaa agtgagcccc tgtatccctt
     4441 ggtagagagg ctacttggca tagtcgacat tatgttaact aacgctataa ataacgatta
     4501 tgttcccaaa acaatatttt atgaactata acaaaaattt gttatcgcat attgtatatt
     4561 ttaattatag gtgtatacca cttaaattta acacaaaggc gtgattattt tgaaacccaa
     4621 aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta gtataggaat
     4681 tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac caattgaaga
     4741 acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa ttcttttaat
     4801 ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta aaatgctcga
     4861 tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg ctgataatac
     4921 gattaaatat atttataccg tcaaaaaaaa tacaaatgga gaatattcag gaagtatttc
     4981 acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag atacaacctt
     5041 attttttcaa gataatagca tttattattc cgataatgga caaattaaac tactttggaa
     5101 atcaccttta agtacagaaa aaaatggtgt ttcttccttt acagaaaaaa atgatcttat
     5161 atccgtaaat ataaaaaata aaattaaaaa tttctctaaa aaacaaaatt ttcctgcggt
     5221 taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt ggcgtatagc
     5281 agaaacccaa acaagttttc cgtggtgtgc tgcttacgta gaagctgcta tcttatgtaa
     5341 caaaacagat gtacatgcta cttcagctag agccattatg cattatacct atcctaattt
     5401 aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa aatatgcaaa
     5461 ttactgtggt gtttatccta aaaaagtagc acgaatacta aacctttcag aggtccaaac
     5521 acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa attatagtaa
     5581 cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc aaacttacta
     5641 tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag gaccagttac
     5701 aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt ggtaaataaa
     5761 cccattgctt tagttatggg tctagtgtta gttcttttct taactagttg ttcaaaaaaa
     5821 caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac taaagatcat
     5881 tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca atataccatc
     5941 ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa tataaatgaa
     6001 caagttttca tttacaaagc aacaaataat ttactaacac tgcgctctaa taaaaaacaa
     6061 ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt gacaacgaat
     6121 gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg atgaatagtt
     6181 gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     6241 tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg gggattcaag
     6301 ggggagcttg cgcccctttg aaaagcggtt atttgcacca aatacgtagg tatttggcta
     6361 gcaaatatag agcttgccaa gcccttttgg tgagctttac tcattgtgcc tttttaggta
     6421 caatggtatt gcgaacgata cgtccgtagg acgtactgaa agggtgggac ttatgagcga
     6481 acaaaatcaa aatttggcta gcgatcattc taaaaaatat acctatcgat ccaagccaaa
     6541 acaaatcagt tttcgagtga gcgaatctga atttgcaaag ctgaagcagt cagctgaagc
     6601 tttgcaaatg agtgtgccgg ctttcgtgaa agccaaggca caaaacgcgc gtctggtgac
     6661 accgaaagtg gcgccagaca ttgcccaggc aatcgcgcgc gatttagcca aagctggagg
     6721 gaatatcaat caaattgcca agtggtgcaa tacacaccaa catgacgttg ctcctggaga
     6781 cgcccaacgt ttatccgaaa acctaaaaat catgcaaaag gagctacaaa agatatggca
     6841 acagttaaag taagtcgaac cacttcctgt agccgagcaa tcaattacgc agaaccacga
     6901 gcaaccatta aaacaggaat aaactgtgac gtcgattatg ccaaaagtga aatgaaacaa
     6961 attcgcatgc tgtatggaaa agacgaccgt gtacaggcac acttattgat tcaatctttt
     7021 cgaccaggag aaattaccgc tgaaaaagct aatcaactag gggcagagta tgcaaaaaaa
     7081 atagcccccg atcaccaaac agctatttac acacatacag acaaagacca tattcataat
     7141 cacattgtca ttaactccgt taatctggag actggaaaaa aatttcaagc tcatggtcaa
     7201 gcatttctaa acaaatgtta tgacctaaat gacgagattt gtcttgcaca taacttacca
     7261 atcactgagc gaggaaagaa accagaaaaa cgaaccatgt ctgaaataaa attaaaagaa
     7321 aaaaatgaac ctgtttggaa agacgaaatt cggattgcga tcgatcaaac aatgaaaaac
     7381 aaaaaaacca gaacctatga ccaattttgt aattccctca aaacattggg gattcattgc
     7441 ttcaatcgcg ggaaaaattt cacttacgaa ttgatccaca aaaagaaaaa agtacgatcc
     7501 aacagactag gaaaggacta cgaaaaggag acgattttac atgagctgga cagacgagaa
     7561 cgaacctata accaacagcg aaccaacgag ctacgaagag ctcgagctag cattcgcgaa
     7621 actgcagaag aagtacgaag agaacaacga actcttacgc ctgaacaacc ggaaattaga
     7681 ccggctcgaa gaaaccatga acaacacgtt gaaagaggaa cagaccacca acaagaatta
     7741 tcgctctaaa acgttaaaag acatagaaaa cgaaataaag aaacaacgtg cttttctcgt
     7801 tccttatacg caagaaagga tacaaactac gattgattta caagttaaaa aactccatca
     7861 agagctatta gaccagcgaa ataaggaaca gaaaaaggtg cttattcaac gatcattgga
     7921 ttttcttgcc cccttatgtt gcttagtcct gacactgttc gcattttggt tagtatgggg
     7981 aggctaaaat ggcattttat ttaccctatc tactcatttt tgttagtatt tcaggaagta
     8041 tttggctcat ctataaaatt tttcaaacaa gatattcatt aaaaggctct aaaattcgat
     8101 ttaagcgatt tttctttctt ggctgtatat tttccttgat aatcgtttca agcggcttat
     8161 tcggcgtttt agagggaaat aagcgcgttt ctagaagtat tttattagga aatgtcactc
     8221 aaaagtatga atcagcgcgt gataaaaaga aaaaagagca agcattagca caaaaaatgg
     8281 aagagtttac cacttgttat gaggaaatga acgatatttt tgtgaatcaa gaaaaacgtt
     8341 tgacggataa aaacatggaa aaactcactc gtctttatca aaacttgcca gaaaaacagc
     8401 aaaaagaggt tcaagacaac tatgagcgaa ccaaaaaaga cgtgcaatat gtgaaagata
     8461 cgaaagtgga agaagcttgt agtgatttat ttggagatac aattcctttt tcaactagcg
     8521 aacaagaacg aaaagaacga caacaatcag tcacatatga acgctatgaa atcttgcttc
     8581 aacaagcaat taacatacaa aatctgacta aaaaagaaac agctttaaat tatttaaaaa
     8641 gcgtcaagga atggttagat cagcaacagc aaaattgaat ttgattgaaa ttcatgttaa
     8701 actaaacatg caaatgttca aacattagat acacgaaagc cccagagagg tcggattctc
     8761 tggggctttt tttaggttga tgtcgccttt actgatttta cttcttgttt cggttattca
     8821 accaataact gaatacagag atacaaactc ctacaagaag ggggcaaata aacagttcaa
     8881 acatcagata cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatacc
     8941 ctacaaaatt aaatctgtgt ggcaatttta agtatctctt atttccgggt tgttagggct
     9001 tgccctgacc gcctgtaggg cgctttaaat tccagataaa gttttttata ctaagactgt
     9061 cttaaaacgg cttagacaag ctcttagagc ctcaaccctc ctttttgaac aatggtttta
     9121 gcaaaggagc tggaggcgac tgcgcaggta taatgtgaga gcgtagcgat cacacctttt
     9181 tggcgaccgt agggagcgag gttttgaatt agcagtttag aaaagttcgc gaacctgttt
     9241 aaagaaaaaa taattgtttt tttaggtggg aaattttgtc tccctctttt ataatctttt
     9301 tttaaacctc tttttaaacc tctttaacgt cggctgtgtc cttactcccc caaggtatac
     9361 aaaattttat aggcatgaaa ttatctgttt ataggcatga aattatctgt ttataggcat
     9421 gaaattatct gtttataggc atgaaattat ctgttttagg tattgttttt agacataata
     9481 tgtcctaaaa tgatgtcaat aaaatcattt agactggagt aacaactaa
//
