LOCUS       NZ_LR135330             1928 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7654 plasmid 7.
ACCESSION   NZ_LR135330
VERSION     NZ_LR135330.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885352
            Assembly: GCF_900639515.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:50:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,874
            CDSs (with protein)               :: 2,874
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 42 of 112
            Pseudo Genes (incomplete)         :: 67 of 112
            Pseudo Genes (internal stop)      :: 21 of 112
            Pseudo Genes (multiple problems)  :: 14 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135330.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7654"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            <1..668
                     /locus_tag="EQB39_RS16040"
     CDS             <1..668
                     /locus_tag="EQB39_RS16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127820655.1"
                     /translation="KFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACD
                     ILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQI
                     VPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDH
                     SEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            868..>1050
                     /locus_tag="EQB39_RS16045"
                     /pseudo=""
     CDS             868..>1050
                     /locus_tag="EQB39_RS16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1631..>1928
                     /locus_tag="EQB39_RS16055"
                     /pseudo=""
     CDS             1631..>1928
                     /locus_tag="EQB39_RS16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     Typing          2..668
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaatttca ccctgacaaa ggtcgtattg attttaatcc aaataaaatt ggcgagtttt
       61 taaaggggtc gatgaaacaa ttcattcatg atttgtttct ggatgcgcat ttctcacgtg
      121 cagatattgc ttgcgatatt ctcggtgttc ctgatgattt tattacccag tatcgaatcg
      181 ttgaccctgt gtcttttaag ccaatttatg gccgttctgg caaattagaa acagcgtact
      241 ggggtagtcg tgccagcgag cgacaagtgc ggatgtacaa caaaaagctt gaacaggaac
      301 gaaaaaaaca gattgtacca aaagaaatcg aaacatggtg gcgtatagaa atgcagctaa
      361 gacgtggcaa agcaacagat tggcatgcga tggttcggga aagtttagat agctttgcaa
      421 gtcctcatta cttgcctgga gatgtaaaac ctgttgatag aattatgata aaagggctga
      481 atcaagatca ttcagagtgg gcgtatatta gcagaaattt gaaatataga ttgcgcaaat
      541 tgttgaaaga agaaagccaa aacgatgagt tgacgaatca tttacgagag acgtttaagg
      601 aatctgcaaa cgacttgaaa atagaattag acacgtggct tttgggtttg gacgtgacag
      661 agaagtgata ccgaataaga aaagtcagat gagtgagagt aaatttacgt caatgtttaa
      721 tgacgtgagt ttacgataag cgattatttt gcgtgaagca aaataaagcg taaaaggagt
      781 tgagagcgtg acacgctttc ggctcctttt ttcgttgtca cttgtgacag cgaataccct
      841 cggagagcat ttggaattgt gacagccatg tcacaattag gaaaatgtta ctctttgtca
      901 aagagtaaca gaaaggttta cactcttttt attagcgaca gcgcgaagga ggggtttaac
      961 ttggggttta gtatttcgtt caaaaaggga acaaaaaata caagcatttc tcataataat
     1021 cgtgagttaa ctgatagcca aaaaatcaat agtttgtctg gtagaaaata attagcagat
     1081 ttacaagtgg gaaataaaaa gctgaaagaa gaaaattgca tttcaaacta cattttctac
     1141 aatagctcta aaatggcata ttttgcgttc tgagcggttt tagcgtttat ttcgtttagt
     1201 tatcggcaca atcggtgaaa cacgttagag gagcgaaaat ggagcctgta aggcgactgg
     1261 tagcggaacg gtcgtgttag attgtgtagc cgatagctga acgaaataaa tgctggcagc
     1321 gccattctat ttcggcagga ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc
     1381 tttttatttt ttctagagga gtgctataat caaagaaaat gagaaaaaag agtaaaaaaa
     1441 tagtcgagca gtgttgacgc actctcgacc tttacggaaa ctacttcacc tagttccgta
     1501 actcaagttc attatacgaa tatttttagg tttcttcaag ctagagattt ttggatgtgg
     1561 atttttagag ttacgtgaac tctggaaatt ctttccaaga atctcttttt tcgttgagga
     1621 ggagctattt ttgagtaaaa aaggcataat cgtaaatgag tgggaaaaac ttaaccgtat
     1681 ttctaacagg cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat
     1741 cgatcgaatt acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg
     1801 agttgtgttt gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa
     1861 gtcggttggt acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat
     1921 cgaaatgt
//
