LOCUS       NZ_LR135322             4374 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7663 plasmid 6.
ACCESSION   NZ_LR135322
VERSION     NZ_LR135322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885360
            Assembly: GCF_900639525.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:47:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 46 of 111
            Pseudo Genes (incomplete)         :: 62 of 111
            Pseudo Genes (internal stop)      :: 22 of 111
            Pseudo Genes (multiple problems)  :: 15 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7663"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            <1..230
                     /locus_tag="EQB37_RS15740"
     CDS             <1..230
                     /locus_tag="EQB37_RS15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070557124.1"
                     /translation="PDEDNYSEMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTD
                     ESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            234..596
                     /locus_tag="EQB37_RS15745"
     CDS             234..596
                     /locus_tag="EQB37_RS15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            818..2224
                     /locus_tag="EQB37_RS15750"
     CDS             818..2224
                     /locus_tag="EQB37_RS15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002317172.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            2671..3639
                     /locus_tag="EQB37_RS15755"
     CDS             2671..3639
                     /locus_tag="EQB37_RS15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            3642..3875
                     /locus_tag="EQB37_RS15760"
     CDS             3642..3875
                     /locus_tag="EQB37_RS15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            3868..4287
                     /locus_tag="EQB37_RS15765"
     CDS             3868..4287
                     /locus_tag="EQB37_RS15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     Typing          2755..3629
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.032"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          819..2221
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgccggatga agataattat agtgaaatgc ttgaacgaat gatggatgtt ctcaactcgg
       61 ttatcaatgc tgatctagca aaatcttcag ttttgactaa tgaatttgtt cataagcatt
      121 tagcgcaaac aactgatgaa tcagaaaaag aattttggtt gaaattaatg agtcatgtta
      181 tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa ggagatgtag tttatgggaa
      241 tgactttagc agatattaaa gcgcaagaag aaaaattaaa aaaagaacaa gcgaaacttc
      301 gccaacagaa aaaacgcttg attgagaaag aaaatgctgc aatgggcaag gctcttcgca
      361 agcgttttgg gtttgaaact tttgctgatt ttgagcggtg gctatcgtcg atttctgacg
      421 atcagcaaca cgaaaaatca gtggataatg agtggcttgc tgatgaaaaa tcagcgccaa
      481 gcgaacgaga ttatcccgat gaatctgacg gcttattcgg agtgataccg aataagaaaa
      541 gtcagatgag tgagagtaaa tttacgtcaa tatttaatga cgtgaattta cgataagcga
      601 ttattttgcg tgaagcaaaa taaagcgtaa aaggagttga gagcgtgaca cgctttcggc
      661 tccttttttc gttgtcactt gtgacagcga ataccctcgg agagcatttg gaattgtgac
      721 agccatgtca caattaggaa aatgttactc tttgacaaag agtaacagaa aggtttacaa
      781 tctttttatt agcgacagcg cgaaggaggg ttttaacttg gggtttagta tttcatttaa
      841 aaaaggaacg aagaatacaa gtatttctca taataatcgt gagttaactg atcgccagaa
      901 aaccaatgat tggcataagc atattgactt cacgaaatcg gatgaaaata tttatttaga
      961 gcagacgaat ctagcagata aatatgagga attgtttggc gaagtggtag aaaaatataa
     1021 tgcgaaacaa aaaagagccg atagaaaaat cgaggactat ttagcgaaag tgcgcaagga
     1081 taaaaagttg gaacctcaac gagaatttat tgtgcaaata ggcattttag atgatttcca
     1141 gacaaatcga gcagatggtt cttctactgg catatcgaaa caacaagctg aacaaaatcg
     1201 agtgattgcc aataaaattt tggttcagta ttacaaggag tttcaagaga gaaaccctaa
     1261 tttggctgtt tataatgctg ttattcataa tgacgaaata agtccgcacc tgcacttgaa
     1321 tattgtgccg gttgctgaag gttataaacg gggtgttcaa aagcagccga gctttaataa
     1381 agcactggtt caacaaggga tacacgctga taaagataat ggtcgagtgc ttttcaataa
     1441 ttttagaaat caagaagttg actctataga acgattaatg aatgattacg gttggaaaag
     1501 ggatattgtc ggtaccaaca aaattaaaga tattcacgaa tacaaagaaa tcatgagtga
     1561 aatttctgaa ttgcgccaaa cggctgtaaa agagcgtgag agcgtttctg atgagttgag
     1621 gtatatttct aggcaaagag aagaaaacga gcgtgataag gctctgatgg cttctgagag
     1681 agcgaagatt gaagaagaga agaaaaatat gctgaatgta aaaaaagaga tggcggtcat
     1741 tttccaaaaa gatatggttc ctttagaaag tttcaagagg cttcctgatg gttcgtatca
     1801 tttatctggg aatgattttg ttgatttgta tcaacgagct acgagagcaa caaatgccaa
     1861 gagaaatgct gataacatga gtgagcaata tttacagatg cttgagcaaa atacagaatt
     1921 aaatgagtat gtagctacat tggaagaaga gcgtcatttg tctggtagaa aacaattagc
     1981 agatttacaa gtggaaaata aaaagctgaa agaagaaaat cgctctttac gccagcgttt
     2041 agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt ttgtctatcc gttttggtct
     2101 tgcagaaaag gatatggggc tttctgagga attagaagtg ttagagcagg agaaaacctt
     2161 attttatcgt caaaacgtct cacagcgtaa attagagcgt tctgaagatg agatcgagtg
     2221 gtagttacga tgtgcagtca acggtaccgg aggcggtttg tgccgaggat aaggagttga
     2281 cggctaaacc actagcctat taagctgaca tttgctaaaa aatggccagt ccattttttg
     2341 gccaacctag cgttagcgaa gtttgggggc gcccttttca aagggtgcgg ttttattttt
     2401 tggatatttg catagctaaa gagaagtgct attattgagt tagcaaacaa atgaactgaa
     2461 aaaagagtaa aaaaatagtc gagcaacgct accaacgttc tcgaccttta cggaaactaa
     2521 tcgcactagc tccgtaactt cttaatcatt atacgaatat ttttagattt cttcaagact
     2581 agagattttt tggtaatgaa tttttaagag ttacgtgaac ttttgaaaat tctttccaga
     2641 agtctctttt tctttgagga ggaacaaaat ttgagtaaaa atggcataat cgtaaatgag
     2701 tgggaaaatt taacagccct atctaacagg cggttaaatg tacaaaatat gtacaccccc
     2761 ttaaaaatgc agtggtcaat tgaccgaatt actatcgttg gaaaactgaa ggaaaatatt
     2821 tattatcaca ctcaaaatga tgtgttgatt ttagattttg agcaattaat gcgggtgaat
     2881 gaaggcaatg gctatttaaa agcggttggg aataacggct ggcaattatt agaccaatac
     2941 gaagaaaata ttgcttatat tgagattttg aaatggcaag atggcaaagg gcggattgat
     3001 tttaacccga ataagattgg tcatttttta gctagttcaa tgaaaaattt cattcatgat
     3061 ttatttctag aaccacattt ctcacgtgcg gatattgctt gcgatattat cgatgtgcct
     3121 gatgagttta ttacacaata tcgagtggtt gatcctgttt ctttcaagcc aatttatggg
     3181 cggtctggga agctggaaac agcttattgg ggcagtcgtg ccagcgaacg ccagattcgg
     3241 ctatacaaca aaaaattgga acaggaaacc aagcgtaaaa ttgtgccacc agaaatagaa
     3301 acttggtggc gactggaatt gcagttaagg cgtgggaaag caacagattg gcatgcaatg
     3361 gttcatgaaa gtttaaatag ttttgctagc cctcattttc taccacttga tgtaaaaaca
     3421 aatgataagg tgatgatttt tggactgatt gctaaccctg aaatgtgggg gtttttagag
     3481 cgtagaatga aatacaaatt gagagatatt ttgaaaagag aaagtcaaaa tgatgagttg
     3541 acgaatcatt tacgagaaac attttcggaa tcagcagatg ttttgaaaaa agaattagac
     3601 acttggcttc aagggttaga tgtaacggag gaggaataag aatggcagaa aaacaaaagc
     3661 attttggtaa gaaaaattgg ttggtgaatt taacaggtga taacgttgtc gttgtttttt
     3721 tggatagcaa aaaaatgtcc ggtaagttac ttgatgtaac gcagtatgag ttgattttga
     3781 aaaataataa aattgattcc ccaatcatta tccctaagca tgctgttaaa tatgtttatg
     3841 gggtagctga aagtaacgga gaagataatg actgatttgc cagaaaaaat gaccataaaa
     3901 aaccttgctg atgagttggg tatatctaaa aatacagtgc gcaatcaact ggtcaatgca
     3961 ggttatgacc ttgctcggta taaagataat ggggttatca tgttagacaa ggaattgatt
     4021 ggtgttgtgc ggtcaattta tcggtcaaaa ggtggtcaat tgactggtga tttaccggtc
     4081 aatgatgcag gcaatgattt cccgttagaa aaagaaaatg aatttttgcg tgaacaatta
     4141 gcggaaaaag ataggcatat ttctgaatta acggaaatga ttcgtgacca acaaaaattg
     4201 actttgcttc agcaacaaaa aaacgatcag ttgttactag aaaatcaaga attaaaaaat
     4261 cgaaaatggt ggcaattctg gaagtgaggt gtgacatgga taaaattgat ttgagagaag
     4321 aaacagtcga tagaattgaa ttgatgtatg aacggttagc tgattatttg aatt
//
