LOCUS       NZ_LR135316             4074 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7240 plasmid 10.
ACCESSION   NZ_LR135316
VERSION     NZ_LR135316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885079
            Assembly: GCF_900639485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,303
            CDSs (total)                      :: 3,206
            Genes (coding)                    :: 3,061
            CDSs (with protein)               :: 3,061
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 55 of 145
            Pseudo Genes (incomplete)         :: 91 of 145
            Pseudo Genes (internal stop)      :: 26 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7240"
                     /db_xref="taxon:1352"
                     /plasmid="10"
     gene            <1..577
                     /locus_tag="EQB75_RS17300"
     CDS             <1..577
                     /locus_tag="EQB75_RS17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_145987364.1"
                     /translation="EPHFSRADIACDIIDVPDEFITQYRVVDPVSFKPIYGRNGKLETA
                     YWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRGKATDWHAMVYESLDS
                     FASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLLKQESQNDELTNHLRE
                     TFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            574..807
                     /locus_tag="EQB75_RS17305"
     CDS             574..807
                     /locus_tag="EQB75_RS17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            878..1225
                     /locus_tag="EQB75_RS17310"
     CDS             878..1225
                     /locus_tag="EQB75_RS17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171346404.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYSE
                     MLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGV
                     IYRNKSKGKEL"
                     /name="hypothetical protein"
     gene            1229..1591
                     /locus_tag="EQB75_RS17315"
     CDS             1229..1591
                     /locus_tag="EQB75_RS17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303404.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKKSQ
                     MSESKFTSMFNDVSLR"
                     /name="hypothetical protein"
     gene            1813..3228
                     /locus_tag="EQB75_RS17320"
     CDS             1813..3228
                     /locus_tag="EQB75_RS17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002303405.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREKNEREK
                     ALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADFVDLYQK
                     ASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSAVVAENKK
                     LKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKTLASQQNAP
                     ERNIGRSENEMEW"
                     /name="plasmid recombination protein"
     gene            3677..>4074
                     /locus_tag="EQB75_RS17325"
                     /pseudo=""
     CDS             3677..>4074
                     /locus_tag="EQB75_RS17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     Typing          3756..4074
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.77e-166"
     Typing          1721..3225
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.207"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaacctcac ttttcacgtg cagatattgc ttgcgacatt atcgatgtac ctgatgagtt
       61 tattacacaa tatcgggtag ttgatccagt ttcttttaag cctatttatg gacggaatgg
      121 aaaattagag acggcatatt ggggatctcg ctcttctgaa cgtcaaattc ggatgtataa
      181 taaaaaactg gaacaagaga agaaaagaaa aattgtgcca aaagaaatcg tgagttggtg
      241 gcggttagaa ttacaattaa gacgagggaa agcgactgat tggcatgcta tggtttatga
      301 aagcttagat agttttgcta gtcctcatta cttgcctgct gatacaagtg tagctgacaa
      361 aatgatgatt actgctttaa caacagaaca tgattattgg gggcaaatta atcgaaagac
      421 aaaatataaa tatcgaaatt tgctgaagca agaaagtcaa aatgatgaat tgacgaatca
      481 tttacgagaa acctttgcag aatcggcaga cgatttaaaa aaagaattag acacatggct
      541 tctagggcta gacgtaacag aggaggaaga aaaatgacag aaaaacaaaa gtattttggt
      601 aagaaaaatt ggttggtaaa tttaacaggt gattacgttg tggttgtttt tttggatagc
      661 aaaaaaatgt ccggtaagtt acttgatgta acgcaatatg aattgatttt gaaaaataat
      721 aaaattgact ctccgattat tatccctaag cacgccgtta aatatattta tgcggtagct
      781 gaaagtagtg gagaagctaa tgactgattt gtcagaaaag atgaccataa aaaatcttgc
      841 tgatgaatta ggtgtatcga aaattaaaga atcggaaatg gtggcagttt tggaaatgag
      901 gtgtaacatg gataaaattg atttaagaga agagacagtt gatagaattg aattgatgta
      961 tgaacgatta gctgattatt tgaatttgcc ggatgaagat aattatagtg aaatgcttga
     1021 gagaatgatg gacgttctca attctacaat caatgtaggc ctagcaaatt cttctttgat
     1081 tactgataaa tttgtttacg gtaaaatagc cgaaacagaa gatgaatctg aaaaggaatt
     1141 ttggctgaat attttaactt atgttatgca attaaatcaa attggtgtta tttatcgaaa
     1201 taaatcaaaa ggcaaggagc tgtaaatctt gggaatgact ttagcagata ttaaagcaca
     1261 agaagaaaaa ttgaaaaaag aacaggcaaa acttcgtcaa cagaaaaaac gattgattga
     1321 gaaggaaaat gctgaaatgg gtaaggcact tcggaagcat tttggatttg aatcttttga
     1381 tgattttgag cggtggttat cgtcaatttc tgacgatcag caacacgaaa aatcagtgga
     1441 taatgagtgg cttgctgatg aaaaatcagc gccaagcgaa cgagattatc ccaatgaatc
     1501 tgacggctta ctcggagtga taccgagtaa aaaaagtcag atgagtgaga gtaaatttac
     1561 gtcaatgttt aatgacgtga gtttacgata agcgattatt ttgcgtgaag caaaataaag
     1621 cgtaaaagga gttgagagcg tgacacgctt tcggctcctt ttttcgttgt cacttgtgac
     1681 agcgaatacc ctcggagagc atttggaatt gtgacagcca tgtcacaatt aggaaaatgt
     1741 tactctttga caaagagtaa cagaaaggtt tacactcttt ttattagcga cagcgcgaag
     1801 gagggtttta acttggggtt tagtatttca tttaaaaaag gaacgaagaa tacaagtatt
     1861 tctcataata atcgtgagtt aactgatagc cagaaaacca atgattggca taagcatatt
     1921 gacttcacga aatcggatga aaatatttat ttagagcaga cgaatctagc agataaatat
     1981 gaggaattgt ttggcgaagt ggtagaaaaa tataatgcga aacaaaaaag agccgataga
     2041 aaaatcgagg actatttagc gaaagtgcgc aaggataaaa agttggaacc tcaacgagaa
     2101 tttattgtgc aaataggcat tttagatgat ttccagacaa atcgagcaga tggttcttct
     2161 actggcatat cgaaacaaca agctgaacaa aatcgagtga ttgccaataa aattttggtt
     2221 cagtattaca aggagtttca agagagaaac cctaatttgg ctgtttataa tgctgttatt
     2281 cataatgacg aaataagtcc gcacctgcac ttgaatattg tgccggttgc tgaaggttat
     2341 aaacggggtg ttcaaaagca gccgagcttt aataaagcac tggttcaaca agggatacac
     2401 gctgataaag ataatggtcg agtgcttttc aataatttta gaaatcaaga agttgactct
     2461 atagaacgat taatgaatga ttacggttgg aaaagggata ttgtcggtac caacaaaatt
     2521 aaagatattc acgaatacaa agaaatcatg agtgaaattt ctgaattgcg ccaaacggct
     2581 gtaaaagagc gtgagagcat ttctgcggaa atgaggtata tttctaagca aagagaaaaa
     2641 aacgagcgtg agaaggctct ggtggcttct gagaaggcga aaattgaaga agagaaaaaa
     2701 tcgattatcg atttggaaaa agagctggct gttgtatttc aaaaagatcc tgtgccactc
     2761 aatagttttg aaaaattatc agatggttct tatcgcttat ctagtgctga ttttgtagat
     2821 ttgtatcaaa aagcgtctaa tgctacgaac agcaaaaaat atgctgatat ggcgaatgaa
     2881 tcgcttggaa atacattaga taaaaatatc gctttatttg ataaaaacat ggcgttagaa
     2941 caagaaaatt cttttttgaa aaatcaacaa aaggttgatg tgtcagcggt tgttgctgaa
     3001 aacaaaaagc tgaaggaaga aaatcgttct ttgcgtcggc ggttaactca actgaaaaac
     3061 gcatttcaaa aatcaatcac tcgtttatca attcgttttg ggcttgtaga aaaagatatg
     3121 ggaatatctg aagagctaaa aatattagat caagagaaaa cgttggctag tcagcagaat
     3181 gccccagaac gtaatatagg gcgttctgag aatgagatgg agtggtagtt atacggtgca
     3241 gtcaacggta ccggaggcgg tctgtgccga ggataaggag ttgacggcta aaccactaat
     3301 ccctgaagct gacatttgct aaaaaatggc aagtccattt tttggccaac ttagcgttag
     3361 cgaagtttgg gggcgccctt ttcaaagggt gcggttttat gtttttggat atttgcatag
     3421 ctaaagagaa gtgctattat tgagttatca aataaataaa ctgaaaaaag agcaaaaaaa
     3481 taaagtcgag caacgctgga cacgttctcg acctttacgg aaactaatca cactagctcc
     3541 gtaactcaaa ttcattatac gaataatttc aggtttcttc aagactagag attgctggtt
     3601 atgaattttt tagagttacg tgaactctgg aaaattcttt ccaccaatct cttttttctt
     3661 tgaggaggaa ctaaatttga gtaaaaaagg cacgatcata aatgagtggg aaaaattaac
     3721 agccccctct aacaggcggt taagtgtccc ttttatggac acccccctaa aaattcagtg
     3781 gtcaatcgac cgaattacca ttgttggaaa tcttaaagag aatatctatt atcacactac
     3841 acatgatgtg ttgattttag attttgaaca gcttatgcgc ctaaatgaag gtaacggtta
     3901 tttaagatct gttagtaata atggttggca gttgttagac caacatgaag agaatattgc
     3961 ttatattgaa attttaaaat ggcaagaggg gaaagggcgt atagacttca atccgaataa
     4021 gattagtcaa ttcttagcta gttccatgaa aaattttatt catgatttgt ttat
//
