LOCUS       NZ_LR135315             4374 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7240 plasmid 9.
ACCESSION   NZ_LR135315
VERSION     NZ_LR135315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885079
            Assembly: GCF_900639485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,303
            CDSs (total)                      :: 3,206
            Genes (coding)                    :: 3,061
            CDSs (with protein)               :: 3,061
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 55 of 145
            Pseudo Genes (incomplete)         :: 91 of 145
            Pseudo Genes (internal stop)      :: 26 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7240"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            complement(<1..719)
                     /locus_tag="EQB75_RS17265"
     CDS             complement(<1..719)
                     /locus_tag="EQB75_RS17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127897261.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFA"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(1166..2572)
                     /locus_tag="EQB75_RS17270"
     CDS             complement(1166..2572)
                     /locus_tag="EQB75_RS17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002317172.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(2794..3156)
                     /locus_tag="EQB75_RS17275"
     CDS             complement(2794..3156)
                     /locus_tag="EQB75_RS17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(3160..3468)
                     /locus_tag="EQB75_RS17280"
     CDS             complement(3160..3468)
                     /locus_tag="EQB75_RS17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(3477..3896)
                     /locus_tag="EQB75_RS17285"
     CDS             complement(3477..3896)
                     /locus_tag="EQB75_RS17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(3889..4122)
                     /locus_tag="EQB75_RS17290"
     CDS             complement(3889..4122)
                     /locus_tag="EQB75_RS17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(4125..>4374)
                     /locus_tag="EQB75_RS17295"
                     /pseudo=""
     CDS             complement(4125..>4374)
                     /locus_tag="EQB75_RS17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     Typing          complement(1170..2572)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctagcaaaac tatttaaact ttcatgaacc attgcatgcc aatctgttgc tttcccacgc
       61 cttaactgca attccagtcg ccaccaagtt tctatttctg gtggcacaat tttacgcttg
      121 gtttcctgtt ccaatttttt gttgtatagc cgaatctggc gttcgctggc acgactgccc
      181 caataagctg tttccagctt cccagaccgc ccataaattg gcttgaaaga aacaggatca
      241 accactcgat attgtgtaat aaactcatca ggcacatcga taatatcgca agcaatatcc
      301 gcacgtgaga aatgtggttc tagaaataaa tcatgaatga aatttttcat tgaactagct
      361 aaaaaatgac caatcttatt cgggttaaaa tcaatccgcc ctttgccatc ttgccatttc
      421 aaaatctcaa tataagcaat attttcttcg tattggtcta ataattgcca gccgttattc
      481 ccaaccgctt ttaaatagcc attgccttca ttcacccgca ttaattgctc aaaatctaaa
      541 atcaacacat cattttgagt gtgataataa atattttcct tcagttttcc aacgatagta
      601 attcggtcaa ttgaccactg catttttaag ggggtgtaca tattttgtac atttaaccgc
      661 ctgttagata gggctgttaa attttcccac tcatttacga ttatgccatt tttactcaaa
      721 ttttgttcct cctcaaagaa aaagagactt ctggaaagaa ttttcaaaag ttcacgtaac
      781 tcttaaaaat tcattaccaa aaaatctcta gtcttgaaga aatctaaaaa tattcgtata
      841 atgattaaga agttacggag ctagtgcgat tagtttccgt aaaggtcgag aacgttggta
      901 gcgttgctcg actatttttt tactcttttt tcagttcatt tgtttgctaa ctcaataata
      961 gcacttctct ttagctatgc aaatatccaa aaaataaaac cgcacccttt gaaaagggcg
     1021 cccccaaact tcgctaacgc taggttggcc aaaaaatgga ctggccattt tttagcaaat
     1081 gtcagcttaa taggctagtg gtttagccgt caactcctta tcctcggcac aaaccgcctc
     1141 cggtaccgtt gactgcacat cgtaactacc actcgatctc atcttcagaa cgctctaatt
     1201 tacgctgtga gacgttttga cgataaaata aggttttctc ctgctctaac acttctaatt
     1261 cctcagaaag ccccatatcc ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg
     1321 atttttgaaa tgcgtttttt aatcgctcta aacgctggcg taaagagcga ttttcttctt
     1381 tcagcttttt attttccact tgtaaatctg ctaattgttt tctaccagac aaatgacgct
     1441 cttcttccaa tgtagctaca tactcattta attctgtatt ttgctcaagc atctgtaaat
     1501 attgctcact catgttatca gcatttctct tggcatttgt tgctctcgta gctcgttgat
     1561 acaaatcaac aaaatcattc ccagataaat gatacgaacc atcaggaagc ctcttgaaac
     1621 tttctaaagg aaccatatct ttttggaaaa tgaccgccat ctcttttttt acattcagca
     1681 tatttttctt ctcttcttca atcttcgctc tctcagaagc catcagagcc ttatcacgct
     1741 cgttttcttc tctttgccta gaaatatacc tcaactcatc agaaacgctc tcacgctctt
     1801 ttacagccgt ttggcgcaat tcagaaattt cactcatgat ttctttgtat tcgtgaatat
     1861 ctttaatttt gttggtaccg acaatatccc ttttccaacc gtaatcattc attaatcgtt
     1921 ctatagagtc aacttcttga tttctaaaat tattgaaaag cactcgacca ttatctttat
     1981 cagcgtgtat cccttgttga accagtgctt tattaaagct cggctgcttt tgaacacccc
     2041 gtttataacc ttcagcaacc ggcacaatat tcaagtgcag gtgcggactt atttcgtcat
     2101 tatgaataac agcattataa acagccaaat tagggtttct ctcttgaaac tccttgtaat
     2161 actgaaccaa aattttattg gcaatcactc gattttgttc agcttgttgt ttcgatatgc
     2221 cagtagaaga accatctgct cgatttgtct ggaaatcatc taaaatgcct atttgcacaa
     2281 taaattctcg ttgaggttcc aactttttat ccttgcgcac tttcgctaaa tagtcctcga
     2341 tttttctatc ggctcttttt tgtttcgcat tatatttttc taccacttcg ccaaacaatt
     2401 cctcatattt atctgctaga ttcgtctgct ctaaataaat attttcatcc gatttcgtga
     2461 agtcaatatg cttatgccaa tcattggttt tctggcgatc agttaactca cgattattat
     2521 gagaaatact tgtattcttc gttccttttt taaatgaaat actaaacccc aagttaaaac
     2581 cctccttcgc gctgtcgcta ataaaaagat tgtaaacctt tctgttactc tttgtcaaag
     2641 agtaacattt tcctaattgt gacatggctg tcacaattcc aaatgctctc cgagggtatt
     2701 cgctgtcaca agtgacaacg aaaaaaggag ccgaaagcgt gtcacgctct caactccttt
     2761 tacgctttat tttgcttcac gcaaaataat cgcttatcgt aaattcacgt cattaaatat
     2821 tgacgtaaat ttactctcac tcatctgact tttcttattc ggtatcactc cgaataagcc
     2881 gtcagattca tcgggataat ctcgttcgct tggcgctgat ttttcatcag caagccactc
     2941 attatccact gatttttcgt gttgctgatc gtcagaaatc gacgatagcc accgctcaaa
     3001 atcagcaaaa gtttcaaacc caaaacgctt gcgaagagcc ttgcccattg cagcattttc
     3061 tttctcaatc aagcgttttt tctgttggcg aagtttcgct tgttcttttt ttaatttttc
     3121 ttcttgcgct ttaatatctg ctaaagtcat tcccataaac tacatctcct tttctttgga
     3181 attaataacg gaaatttggt ttaactgcat aacatgactc attaatttca accaaaattc
     3241 tttttctgat tcatcagttg tttgcgctaa atgcttatga acaaattcat tagtcaaaac
     3301 tgaagatttt gctagatcag cattgataac cgagttgaga acatccatca ttcgttcaag
     3361 catttcacta taattatctt catccggcaa attcaaataa tcagctaacc gttcatacat
     3421 caattcaatt ctatcgactg tttcttctct caaatcaatt ttatccatgt cacacctcac
     3481 ttccagaatt gccaccattt tcgatttttt aattcttgat tttctagtaa caactgatcg
     3541 tttttttgtt gctgaagcaa agtcaatttt tgttggtcac gaatcatttc cgttaattca
     3601 gaaatatgcc tatctttttc cgctaattgt tcacgcaaaa attcattttc tttttctaac
     3661 gggaaatcat tgcctgcatc attgaccggt aaatcaccag tcaattgacc accttttgac
     3721 cgataaattg accgcacaac accaatcaat tccttgtcta acatgataac cccattatct
     3781 ttataccgag caaggtcata acctgcattg accagttgat tgcgcactgt atttttagat
     3841 atacccaact catcagcaag gttttttatg gtcatttttt ctggcaaatc agtcattatc
     3901 ttctccgtta ctttcagcta ccccataaac atatttaaca gcatgcttag ggataatgat
     3961 tggggaatca attttattat ttttcaaaat caactcatac tgcgttacat caagtaactt
     4021 accggacatt tttttgctat ccaaaaaaac aacgacaacg ttatcacctg ttaaattcac
     4081 caaccaattt ttcttaccaa aatgcttttg tttttctgcc attcttattc ctcctccgtt
     4141 acatctaacc cttgaagcca agtgtctaat tcttttttca aaacatctgc tgattccgaa
     4201 aatgtttctc gtaaatgatt cgtcaactca tcattttgac tttctctttt caaaatatct
     4261 ctcaatttgt atttcattct acgctctaaa aacccccaca tttcagggtt agcaatcagt
     4321 ccaaaaatca tcaccttatc atttgttttt acatcaagtg gtagaaaatg aggg
//
