LOCUS       NZ_LR135314             5204 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7240 plasmid 8.
ACCESSION   NZ_LR135314
VERSION     NZ_LR135314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885079
            Assembly: GCF_900639485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,303
            CDSs (total)                      :: 3,206
            Genes (coding)                    :: 3,061
            CDSs (with protein)               :: 3,061
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 55 of 145
            Pseudo Genes (incomplete)         :: 91 of 145
            Pseudo Genes (internal stop)      :: 26 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7240"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(133..549)
                     /locus_tag="EQB75_RS17230"
     CDS             complement(133..549)
                     /locus_tag="EQB75_RS17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(551..832)
                     /locus_tag="EQB75_RS17235"
     CDS             complement(551..832)
                     /locus_tag="EQB75_RS17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(1208..1732)
                     /locus_tag="EQB75_RS17240"
     CDS             complement(1208..1732)
                     /locus_tag="EQB75_RS17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060800956.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(1749..2717)
                     /locus_tag="EQB75_RS17245"
     CDS             complement(1749..2717)
                     /locus_tag="EQB75_RS17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            complement(3887..4330)
                     /locus_tag="EQB75_RS17255"
     CDS             complement(3887..4330)
                     /locus_tag="EQB75_RS17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(4303..4803)
                     /locus_tag="EQB75_RS17260"
     CDS             complement(4303..4803)
                     /locus_tag="EQB75_RS17260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060800863.1"
                     /translation="MNYNKNLLSHIVYFNYIIIGVYHLNLTQRRGYFETQKENLSRSPL
                     FLDDWNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGY
                     GNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTI
                     TNW"
                     /name="hypothetical protein"
     gene            complement(5021..>5204)
                     /locus_tag="EQB75_RS17700"
     CDS             complement(5021..>5204)
                     /locus_tag="EQB75_RS17700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232043854.1"
                     /translation="WGAAPISKYRLFNSVVTQKNRTYSEIKKDCSNEQPIKNNERLFLK
                     KQRAYEALPKIYHIL"
                     /name="hypothetical protein"
     Typing          complement(1754..2717)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aatgcaaaaa gaaggattcg cacaagcgga tttttctttt tttgtgcaag ttaccggtaa
       61 cttgcaaatc ttgctggaag actgatccct aaaaagaaat cgaggaattt ttttagggat
      121 cggtttcttt ttttatcttt ttagaaagtt tgttgggtca aaatttccag aagtaaaggt
      181 ttgattggtt tctttttgac gatttttggg ttgatttcgt cgggaaggat tgagttgatt
      241 ttcaattttt tctttcactt cttgatacac tttagaattt gtaccgataa gaacaccagt
      301 atcttttaga tataaatcag caaattctgc catgatttct gtcatgatag aaatttcttt
      361 tcccatggca ttgagttttg cttggttact ggttgtggtt gtattggtat caggttcata
      421 actcatacat aaaaaacgaa tggcttctga ataattgttg acgtctggat ttttttcgat
      481 gatggaatct aaaacgtcaa cgtgactggg ataaagggtt acgtgttttt gaacagaagt
      541 ttttctcatg ttatattcct ccgataagtg tttcaatgag ttggttgttt gtgtgaacat
      601 agtcattcag tatgttcgta ttttctgttt gaatggcaat aaatttttcg agtaataaat
      661 cgaattgatc ttttttgtaa ttcataagtt gacggtcaaa atcactttcg attagaactt
      721 ttagatattt atttcgactc cattttttgc ccgttttggt tgtgagttcc tgacaacgtt
      781 tatctattct tgcaataatt ttgggattga tatttcgaac taaaatgtcc atggaaattt
      841 tcctttcgta tcagcgtata atttatgtat accatacaaa tatagtattt gtatagctat
      901 ttttcgccaa aacactatac atagcctagc cacaatggca aaaaagtgat agcaaatttt
      961 tttctgagaa acgtgatagg cacagcctat ctagcacctt tcgtaagaaa gtgataccac
     1021 gtttccttat gctctttcaa aattcagaaa aatcagctgt aaaacgagtg cagtccgagt
     1081 aagattcaac gaaaaaacgg ggattggggc gccaccaaaa tatcgcaaca tgggtccatg
     1141 ttgctctgct cgcaaaagca aaaagaagaa taaaaccagc aatttttatt aatttaaatt
     1201 gctggtttta ttgccagaat tgccaccatt ttttctccgt ctttgattgt ttttctatcg
     1261 attctgtttc ttcagaaaga gtcgttgatt tattttccga agattcttcc gtataagtca
     1321 actgtagttg ttcctgcagt ttctcgattt gttggttggc ttgcagggtg agttgttgtt
     1381 ggtggtcgag taatttttgc atttgtttta tttgctcgtc tttttcttgt agttgcatat
     1441 ctttttgatt taactgttgt tctaggagag aaaggacttc taattcggga acttggttgg
     1501 cgtttttgtg ggtttcggtt ttcgtttttt cgccaactag ttgttgattt gagttttcgt
     1561 tttcgttaaa aaacattgat tttatagcct tttgtccggt ttcattaatc acaaacttgt
     1621 ttccgatttt cgaaaaccaa agttttcgat tttcgtcaga aacttttttg ttaattgctg
     1681 ttttactaac tcctaattct tcagcaagtt ctttgattgt tttttctgtc ataaaatcta
     1741 aaaacccctc aatctttgaa taagttttga agattgtcta ataagtcttt ttttagttct
     1801 gaaggaatat cggaatttat ctctgatttt tgtgcattat tttctttatt ttgggtattt
     1861 tgttctcttt ctaacttttc taactgttgt aaaagttcca attttcgctt ttgttctttg
     1921 attttttctt gtaatgaagt aggtgctttt tcagattgaa cttgttggtt agctagttct
     1981 aatgctttgt tttgttcttc ttttgttggg gcatgtttgt taaagtcgtc tgattttttt
     2041 atctcaggtt taaatgtaaa ttgatagcca atgacaggct ttccccgtcc ttttccgtat
     2101 ttctttttga ttgttaaacc tttaaagatt gcggttagtt cttgtcgtat tggattgaac
     2161 acacgttgat caatcgtacc ttggttgtaa cttttaggaa tatcaagtaa ttcatgaaag
     2221 tcttcttttg aaaaataagc gaaacctttg gttctgtatt gttttagtaa tcgaaacatt
     2281 gtttttgaat aactgctttc taattcattg aattgttgga gagaatagcg cacccattct
     2341 tcaagattat taagtaaggg taaggctttt tcatgaactt gtatttcagc aaaaggaata
     2401 tcagattttc cgtcgatttt aaattgatta aataaaacaa aacgttcacg ttgcaaaccg
     2461 tcagcacttc ttctaccaaa acgtaaatcc attaatttgt catacgttgt ttctaaatca
     2521 tctatgaatc gaacatttgc agttgcttta taattgctta aatcttttaa ttgctcgaat
     2581 gtaaattgta cttttttatc acctacatca cgcattcttg atacgataga aaagaacaaa
     2641 ttcatttcga ctggtgtaaa ttttcgcaac ggaattgtat ttaattcatg atgatacttt
     2701 acgatttcat tagacatatt taaaatagct cctttcttac tcgcacaaga gatacacgtt
     2761 atgaaataca aaatgcctgt ttatctacat cttatattaa catcgtcgat gtactaattc
     2821 aatgtacaaa cagacaaaac gatgtagata aacagacaaa acgatgtaga taaacagaca
     2881 aaacgatgta gataaacaga caaaacgatg tagataaaag cgtcattccc ttgggagagt
     2941 aaggatatag ccgacggtaa agaggtttaa aaagaggttt aaaaagagat tataaaagag
     3001 ggggacaaaa tttcccgcct aaaaaaccac ttcttttttc tccaaacaga ttcgcgcatt
     3061 tttctcaact gggaatctta ctccctacag tcgcttaaaa aggtgtgatc gctctgctct
     3121 cacattatac tgcgcagtcg cttccggctc ctttgctaaa aatgttcata aggaactctt
     3181 tgagaagcta tgagccttcg tttgagacgt tttaagacag tcttagtata aatcccttat
     3241 cttgttctta aagcgcctta caggcggtca gggcaagccc taacaaccct aaagttgtct
     3301 gaatgccctc taaaattcga tttaagcact cttaaaccta ctcttatata tttatatact
     3361 tagacgtttc aaacgtctta aaacgcaaat tagagcttga taaagatatt tgctcgtgtg
     3421 ccgacttttt taaaagtcta ggcacacgcc atgacaaagc tgaaataaac tgcttcagct
     3481 ttgtcatggc ggattcgctc acttgaaaaa gtgttcattt cgctcgatta ttagcatata
     3541 ttttttaact ttttttgcta gccaaatttt gcattagttc gctcataaat cccacccttt
     3601 cagtacgtcc tacggacgta tcgttcgtaa tgtgaaagta cctttaaagg tactttctac
     3661 aaaactcact aaagtgtttg gcaagctcta tatttgctag ccaaatacct acgtatttgg
     3721 tgcaaataac cgcttgtcaa aggggcgcaa gctccccctt gaatccccct caaaaaccac
     3781 gctccacaat acaaaaaagg ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt
     3841 tcaccttcgg tgaaattatt gttttgtaat atcaaacaac tattcatcaa tcagctttaa
     3901 aaaattcaat tggtttagct gtcccttctg tcccatcatt cgttgtcaaa ataaatacga
     3961 gcttttttgg agaatccaaa ctaaaagtgt aatctaattg ttttttatta gagcgcagtg
     4021 ttagtaaatt atttgttgct ttgtaaatga aaacttgttc atttatattt aaatttttat
     4081 caatgatttg aattttatga ttgttctctt ttactaagat ggtatattgt tctatatttt
     4141 ttgaagtatt taaattagtg attactagac gttctgaatg atctttagtt tgccaagttc
     4201 ctagaaaagg aatcagatca gtcttttgta tttcttgttt ttttgaacaa ctagttaaga
     4261 aaagaactaa cactagaccc ataactaaag caatgggttt atttaccaat ttgtaatggt
     4321 attgtcccaa gtaaaaccac ctccaggtac aggaattgta actggtcctg catttgcact
     4381 tattacagtt gggtagttat accaaggatt ccacacatag taagtttgat aaccaccttg
     4441 attgacccac ccaaataatg caagtgcatg acggctgtta ctataatttc cataaccagc
     4501 agttcccata taaatggcat ttccttttct tagttgtgtt tggacctctg aaaggtttag
     4561 tattcgtgct acttttttag gataaacacc acagtaattt gcatatttta ttatttgatc
     4621 ttgattaaga ctttttgatt caatttgact attccaatca tcaaggaaaa gaggagacct
     4681 agataaattt tctttttggg tttcaaaata accacgcctt tgtgttaaat ttaagtggta
     4741 tacacctata attatataat taaaatatac aatatgcgat aacaaatttt tgttatagtt
     4801 cataaaatat tgttttggga acataatcgt tatttatcga gctagttaac ataatgtcga
     4861 ctagcccaag ttgcccctct accaacgggt gcagggacgg actttgtgat ttctcattgg
     4921 tctagttaaa acacacgtac aaaagcttga ttttccgcta ttgtctgttt acgattcttt
     4981 gtgaatctta accgattcct ttttagaaac ttgttatttt tcataaaata tggtaaattt
     5041 ttggcagcgc ttcataagcg cgctgctttt tcaagaataa acgctcatta ttttttattg
     5101 gctgctcgtt agagcagtct ttttttattt cagagtacgt tctatttttt tgagttacca
     5161 cgctattaaa taaccgatat ttggagattg gggcagcgcc ccaa
//
