LOCUS       NZ_LR135312             7985 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7240 plasmid 6.
ACCESSION   NZ_LR135312
VERSION     NZ_LR135312.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885079
            Assembly: GCF_900639485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,303
            CDSs (total)                      :: 3,206
            Genes (coding)                    :: 3,061
            CDSs (with protein)               :: 3,061
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 55 of 145
            Pseudo Genes (incomplete)         :: 91 of 145
            Pseudo Genes (internal stop)      :: 26 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135312.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7240"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            <1..492
                     /locus_tag="EQB75_RS17125"
     CDS             <1..492
                     /locus_tag="EQB75_RS17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="WP_145987362.1"
                     /translation="GGTTGGSDIIARILEQNYGITMGRSLLAFDIIVLLASLTYIDLKR
                     MMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTGLQRGVTFLSGEGAF
                     SGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQGFTYLKPQTRLLKKL
                     "
                     /name="YitT family protein"
     gene            complement(574..1155)
                     /locus_tag="EQB75_RS17130"
     CDS             complement(574..1155)
                     /locus_tag="EQB75_RS17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="WP_002289878.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVHF
                     VEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFENA
                     WQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALEDGDR
                     DAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
                     /name="ReoY family proteolytic degradation factor"
     gene            complement(1161..2420)
                     /locus_tag="EQB75_RS17135"
     CDS             complement(1161..2420)
                     /locus_tag="EQB75_RS17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_002289879.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELLA
                     IGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQSL
                     LAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYETLLA
                     SGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQLKENQ
                     QAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELYHLASE
                     NAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQMEETANPY
                     AEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRAQELLTYY
                     LTLEPGDMEALSLLDDLTER"
                     /name="tetratricopeptide repeat protein"
     gene            complement(2422..3468)
                     /locus_tag="EQB75_RS17140"
     CDS             complement(2422..3468)
                     /locus_tag="EQB75_RS17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002294410.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDLF
                     ADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIMDL
                     AGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQTIS
                     ERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFYQLEA
                     ENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFFVWLDL
                     LGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRTMTVFQQ
                     IVSEIRGN"
                     /name="hypothetical protein"
     gene            3704..4999
                     /locus_tag="EQB75_RS17145"
     CDS             3704..4999
                     /locus_tag="EQB75_RS17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="WP_002297185.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTC
                     RNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFL
                     VTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLIKVR"
                     /name="ISL3-like element ISEfa5 family transposase"
     gene            complement(5193..5648)
                     /locus_tag="EQB75_RS17150"
     CDS             complement(5193..5648)
                     /locus_tag="EQB75_RS17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002290187.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSEF
                     VEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPDLV
                     YFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
                     /name="hypothetical protein"
     gene            complement(5802..7010)
                     /locus_tag="EQB75_RS17155"
     CDS             complement(5802..7010)
                     /locus_tag="EQB75_RS17155"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="WP_002294414.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIHD
                     VDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDHVE
                     FVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDALRM
                     MRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIHAFIE
                     TECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLKSWKCS
                     NQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDAVGESYD
                     RLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQVLDFLSKE
                     KLI"
                     /name="CCA tRNA nucleotidyltransferase"
     gene            complement(7122..7454)
                     /locus_tag="EQB75_RS17160"
     CDS             complement(7122..7454)
                     /locus_tag="EQB75_RS17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="WP_002290045.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELARE
                     VNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDHNR
                     FERKDE"
                     /name="nucleotide pyrophosphohydrolase"
     gene            7602..>7985
                     /locus_tag="EQB75_RS17165"
     CDS             7602..>7985
                     /locus_tag="EQB75_RS17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="WP_145987363.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLIL
                     RALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEINLQ
                     HDLLIVSLLAGLVAGVGSGIVYRM"
                     /name="YitT family protein"
ORIGIN
        1 ggtggaacaa caggcggcag tgatattatc gctcggattt tagaacaaaa ttacggcatc
       61 actatggggc gttccctatt ggcatttgat atcatcgtct tacttgcttc attaacctat
      121 atcgacttga aacgtatgat gtatacgttg attgtttcgt atgttttcag tcgtgtgatt
      181 gattcgatct tagatggcgg atattctgca aaaggtctac ttgtcgtctc caacaaaagc
      241 gaagagattg caccactact gatgaccggt ctgcaaagag gtgtgacttt cctttctgga
      301 gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa
      361 ttaactgaag ctaagaggat cgtccacgag attgacgaaa aagctttcct ttcaatcatc
      421 aatgtgcatg aagtggaagg gcaaggcttt acttatttga agccacagac aagattactg
      481 aaaaaactat aagaaaaaag acaaagatag gctatgagga aagattccac atccagctga
      541 cctttgtctt ttttagtact atactatttt taactaattg ttatgttcac ttacccggtt
      601 acttttcttt tgtgtctgca aaaggatctt tcggttaact tcatcagata atttcaaaaa
      661 agcatcccta tctccatctt ctagtgcttg atcgatttga gcataaagca tttttagttt
      721 tgcctcttct tctttttctt gaaaatatcg ttcaattaat tcagtatctt cttcactgac
      781 tgaagaatcc caagaagaaa atggattgtc ttctaagata gatagatatt ctcttgtctg
      841 ccaagcattt tcgaatatac attccacata taaaggcttt ttccaattca aacggatttc
      901 atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc
      961 cccttcaaca gatagatcac tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac
     1021 aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga taattcaaga tccaaaaaac
     1081 ttctcttcgg ctaaaagata catgattgac catccacgtt aaaaaattct tcttttcccg
     1141 tacatcaatt agcatttttc tcacctctct gttaggtcat ctaataatga cagtgcctcc
     1201 atatctcctg gttcaagtgt taaataataa gtcaataatt cttgcgctct tttcagctgc
     1261 ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg tgatagttca
     1321 tgataagcct gttcatagtg tacagctgca acggtaaagt cttctaattc attataagca
     1381 tgggctaggt tccattctgc ataaggatta gcagtttctt ccatctgatt gatcacttgt
     1441 atcacatctt catatctttc atcatccaag taaagattac ttaaagtaag taatgtttca
     1501 tcttgtttgt ctcctacttc taaagctttc agtaaaagtt cttctgctcg ctgtctatca
     1561 tgcagacgga aagcattttc cgaggccagg tgataaagtt cgacttggta aggattttct
     1621 ttgattcctt cttctaacac tgtttgtgct tcttcaatca tttcttcttc ttgaagagct
     1681 tgtcctagat atagatataa cgattgataa tgcggattaa cttctcgaag ttcttccaga
     1741 tagttgattg cctgctgatt ttctttcaat tgaagattcg taaaagctag ctggaacagt
     1801 cgatcatctg tcctttcttc tttcagtgct tcttctaaag gtacgatagc tgcttcgaat
     1861 tctccttgca tacttaagga ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca
     1921 tttatcccgc ttgccaacaa tgtttcataa accactgctg cctctttgaa acgatcaact
     1981 gaaaaataca attcagctag cgcaaactgg atcaatggtt catcaggaag caacccagct
     2041 gcttctttca attttgcttc actcacttca gggattccga taacttggta caaatcagct
     2101 atcgccaata agctttgcgg atagtaatca ctttgcttat ccactttttc caggtattca
     2161 aaagctaagt cgatctcatt atcttcgatt gcaatttctg ctaacgggat gttcagtccg
     2221 tcattttctg ggtattggtt gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag
     2281 ccaatagcca gcaattcttc gcctagttca gctaatatat ctgctgggtc ttcttttaat
     2341 gctttttcta aaaagatttg tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt
     2401 ttttcgctat ttgtattcat ttcaattccc tcttatctca ctgacgattt gttgaaatac
     2461 cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg tatccgtcta ttatacctat
     2521 tttttctaaa acaagtattt ttttgctttt ttctgccaaa aaaagaactt tttccaaata
     2581 ctccatcttt gaaatctgtt ccagccagtg gattggatag ccaagcaagt ccagccacac
     2641 aaaaaagttt tttagttgga aatcaaaagg atatgcttgg agattccaca cgaaatgaaa
     2701 catcattcct aaaaattttt tcatactagc agaaagtaga tggccatttt cggtcagata
     2761 aaaagcttgt tcaaacagtt taccataatt tgacaggttt tctgcctcta attgataaaa
     2821 atagatcatt tcttcgatca tcccggcaaa cggccgagtc gttaattgct ttttatttgg
     2881 atagttttta tacagatttt gcaaaaaacg atgatcacaa atcattccgc aacagataaa
     2941 agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg ctgattgttt gatcataaac
     3001 gatgcgttct ggcagattga tggtttgcag cactacacga tttggtagct gcaaaatcgt
     3061 ccgctgcggt gttaatgctc ggcttagaga acggatcgat acagggatca tccatagtga
     3121 tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga tccatgatcc cttcatttcc
     3181 aaatccgatg ataaacatgg attgattttc gggatagcgt ttgacgaaat caagcaggtt
     3241 ctttaattct gttaaatgat tgcattgagt gttggcacaa atataccagt cattgtttgc
     3301 ttgatttgca aataacgggt tgatcttatc tgcaaacaaa tcataatagc gctgattcgt
     3361 taaaaataag attggctgat caatggaaag ttctcttagc tgactgacta gcgtttctcc
     3421 gtaaaacact ttcgtcaacg catcttctac ttgataatca agttccatac gcatccctcc
     3481 tcttcttatt tttattttac cattagtaag caaattctag aaaagataag agcaaagctc
     3541 acctcataac ttggaaataa ataaaaaggc tctataattt atgggactga tgaatcagaa
     3601 ttgattcttc agccccattt ttattttagg tattaaaaaa catatcgttc tctagtatga
     3661 ttaaatcacg acaaaataaa caactgagag gacgatatgc tatatgaatg attctatcaa
     3721 aaaaatgctg cgattaatag agaaagattt gatgattaca gaggtctctt acgagacctt
     3781 tcagaagaaa aagacgttga tcgtcgatgc tgttttctca cctgctcccc atacctgtag
     3841 aaattgtggt tctactgtgg tagatggaaa cgggaaagta attgtagtga aaaatgggaa
     3901 gaaggaaacg attgtccgct ttgaacaata caatcacatg cctttggtta tgcgcttaaa
     3961 aaagcagcgc tatacctgta aaaactgtcg aacccattgg acggctcaaa gttattttgt
     4021 tcaacccaga cattcaatcg cgaatcatgt cagatataaa attgcttctt tactgactga
     4081 aaaagtatct ttatctttta ttgcgaaaaa ttgtcaggta tctttgacca ccgttattcg
     4141 tacgttgaaa gagtttaaaa gctacttacc aaagcaatct aagaggattc tccccagagt
     4201 attgatggtt gatgaatttc gttcgcacgc ttccatagaa gataaaatga gctttatttg
     4261 cgcagatggc gaaacaggac aattaattga tgtcttgcct acgcgtaaat tgcctcgatt
     4321 aacaagctat ttcttaggct gcgctaatcc tgaagaagta aaatttttag tcacagacat
     4381 gaacgccgcc tacttccagc ttaccaaacg tattctgcca aatgcgaaag tagtgattga
     4441 tcggtttcat attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat
     4501 gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg
     4561 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
     4621 tcgagcacca aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt
     4681 ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga
     4741 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg
     4801 ctttcgggaa aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat
     4861 ctatccttac accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg
     4921 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
     4981 attaatcaaa gtaagataac aaaaaatctg agccagaatg aggttcattc tgacccagat
     5041 ttgattttgt acaacccatc agtccttatt gacaaagagc ccaaaaatct tgtcacagtc
     5101 ccttattctg aataaacggt gtggaagaga cagcctatca gtagtaataa gacacatctc
     5161 tttcacagcc tcatttttgc gtaaaaatcg atttattcga aaaattcccc gtatacatct
     5221 tctacacgtt gatctggatt gatcacgata tacaagttac ctgttttttc gctgattttt
     5281 gttgattgat attgattgtc taatccttct gttcctttat ctagatatgg gaagtacaca
     5341 agatcaggtg tgccatttcg gtaaaggatt tccattcgct cgatatcttc gtatttcaat
     5401 gcgcgattaa acatgcccat ttccagtccg cctaggttga tatcatgtgt cgcaactgcg
     5461 tccccttcat ggaagatttc gattttaaat ccagcacacg gatggatttc aacaaattca
     5521 ctgccattga ttcgtccaaa acttgttgtg atttgtttga tccaaagatc gccgatatga
     5581 cggcggttga tcgtccaggt ttctccattt ctaaaataaa attttagcgc tgtcatttct
     5641 ttactcatta aaaagactcc tttatgatgt aatttattct tgtactacct cagtttagca
     5701 caaatttatg taagtggtca catcttatgt tttttcttct aatgacgtat ctgcccactt
     5761 taagtagcca acaaagctga gcagatacag tctagtgttt tttaaatcaa tttttctttc
     5821 gataaaaaat ccaaaacctg ttttttatca ttttttattc tttgttctac gacggctttt
     5881 tcagctaatt gcaaagcttc actaacccac ttaccaggtt tttttccagt ttcttctagc
     5941 aagattttac catctactgc taattcgctt aatgacttga taggtaaacg atcataggat
     6001 tctcctacgg catccatctg aggtgtttcg tcaaaataga tcatcaattc ttctacttgc
     6061 agtgcatgtt ttttgcctaa gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt
     6121 tgtcgtagtc cttggacaag tgcttgaacg tctttaatca tttggttaga acacttccac
     6181 gatttcaaaa aggaacgaat ctcattttct tttagatcca gttggctgat caataatgcc
     6241 catgcttggc tctcttcttt caatgggacg gatggtaatt cagcaaaacg taaaagtgct
     6301 tcgccttctt ctttcaaacc gggacagcaa atgtagcact ctgtttcaat aaatgcatgg
     6361 attcccgcgg cccgttcatt tcccaagagc atcttgatga attccacatt tatacgttct
     6421 acagatattt tacctaatag ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct
     6481 aatttgaacc ctagttgact gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca
     6541 tggaaccgct catgaggatt gcctacagct cgcaagatct gttcttctag atcttgcaga
     6601 ccatcgaaca aatcgatgat ttctccatct tctttcaaag caaacgcatt gatcgtaaaa
     6661 tctcttcttt tcaaatcttc tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg
     6721 tagtcctgat aagttgattc cgtccgaaat gtggtgatct catattgttc atcattttct
     6781 aataccaaca cagttccatg ttcgatccca atatctatcg tttttggaaa aagctgtttg
     6841 atttcttctg gaaatgcgct tgttgcgata tccacatcat ggattggctg gttcagcaag
     6901 atatcacgaa cacttccacc tacaaaataa gcttcatatc ctgcattctc taattttttc
     6961 agaactggaa ttgccttttg gtattccaga ggtaattgtt ttaatttcat ttttccaacc
     7021 tttcctgcgt gtttcttgtc ctgagataaa gactcagttg catggtgact gtttgacaag
     7081 tccgtttcag agtcttcaag gccaggacca cttcatcttg tttactcgtc cttacgctca
     7141 aagcgattat ggtctctttg attgaatttt tccatgtttt tttcaaaaac ttcagttaag
     7201 tcgatattca atgaattagc catgatcatc gtaacgaata aaacatcacc aagttcttct
     7261 gctaccgagt taactggttc agaagccttt ttgctttttt ctccataagt gtggttgact
     7321 tctcttgcca attcacctac ttcttcagtt aatcgagcca tctgagctaa tggagagaaa
     7381 taccccacct tgaattgctg gatatattca tccacttcct gctgcatact gtataaagaa
     7441 cgctttttat ccatcttttt ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct
     7501 agataagagc aattgagaaa atcgaatttc catgctacat ttaggaagga atttctaaat
     7561 ggaaattctg tgaaaataca taattgaaag ctggaattca tatggtcaaa cgaaaaacag
     7621 taaaagaagt tctttttatc attataggaa caagtattta tgcatttggt cttgtttact
     7681 tgaacatcgc taatcattta gcagaagggg gagtctcagg gatcaccttg atcttaagag
     7741 ctttatttgg tattgatcca gcttatacga ctttattaat caacatccca ttgattttac
     7801 taggaggaaa gatcctcggt aaacgttcct tggcctatac tgttctaggt acactttctt
     7861 tgtcattctt tttatggatc tggcaaaggg ttccactaga aatcaattta cagcatgatc
     7921 tactgatcgt ttctctactt gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc
     7981 gcatg
//
