LOCUS       NZ_LR135306             3124 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7429 plasmid 10.
ACCESSION   NZ_LR135306
VERSION     NZ_LR135306.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885211
            Assembly: GCF_900639465.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:59:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,289
            CDSs (total)                      :: 3,202
            Genes (coding)                    :: 3,086
            CDSs (with protein)               :: 3,086
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 44 of 116
            Pseudo Genes (incomplete)         :: 73 of 116
            Pseudo Genes (internal stop)      :: 24 of 116
            Pseudo Genes (multiple problems)  :: 20 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135306.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7429"
                     /db_xref="taxon:1352"
                     /plasmid="10"
     gene            <1..514
                     /locus_tag="EQB73_RS17195"
     CDS             <1..514
                     /locus_tag="EQB73_RS17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347621.1"
                     /GO_component="GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="WP_127841146.1"
                     /translation="VLYIARNPVTIESVRKKIKRKLGNNSVAHLEIIDHIKGSYEYLTH
                     ESKDAKEKNKHVYDKKDILHINDFDIDRYVTLDESQKKELRNQLFDLVNHCGIVNMKEL
                     AAFLDWRGQEYGITNQRDVVDVIASNSNLFRMMFDANYQNGFRPQYAKRIDSETGEMIE
                     GVNKHVE"
                     /name="Rep family protein"
     gene            complement(602..1018)
                     /locus_tag="EQB73_RS17200"
     CDS             complement(602..1018)
                     /locus_tag="EQB73_RS17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806812.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNQPKNHQKGTNKTVTSSNFDPTKFLKR"
                     /name="hypothetical protein"
     gene            complement(1021..1305)
                     /locus_tag="EQB73_RS17205"
     CDS             complement(1021..1305)
                     /locus_tag="EQB73_RS17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1670..1894
                     /locus_tag="EQB73_RS17210"
     CDS             1670..1894
                     /locus_tag="EQB73_RS17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225471215.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLKYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            2018..2197
                     /locus_tag="EQB73_RS17215"
     CDS             2018..2197
                     /locus_tag="EQB73_RS17215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322661.1"
                     /translation="MEKDIYKQLTRQIQKEKEKGLLDHLDELELKADEINKNRMSAKEK
                     GIKLKDSSLQSDKS"
                     /name="hypothetical protein"
     gene            2686..2835
                     /locus_tag="EQB73_RS17220"
     CDS             2686..2835
                     /locus_tag="EQB73_RS17220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347622.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKKS
                     EKGN"
                     /name="hypothetical protein"
     gene            2916..>3124
                     /locus_tag="EQB73_RS17225"
     CDS             2916..>3124
                     /locus_tag="EQB73_RS17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415118.1"
                     /GO_component="GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="WP_232044224.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMDE
                     KTNKKDWTSSDVIRNGKHYVKPHY"
                     /name="Rep family protein"
ORIGIN
        1 tgtcctttat attgctcgca atcctgtaac aatcgagagc gtaagaaaga aaatcaagag
       61 aaaattggga aataattcgg ttgctcactt agaaataatt gaccatatca aaggctcgta
      121 tgagtatctt acgcatgaat caaaagatgc aaaagaaaag aataaacatg tatacgataa
      181 aaaagatatc ttgcatatta atgattttga tattgatcgt tatgtaactt tggacgagag
      241 tcaaaagaaa gagttgagaa accaattgtt tgatcttgtt aatcattgtg gaatagtcaa
      301 tatgaaagag ttagctgcat ttcttgactg gagaggtcaa gagtatggaa tcactaatca
      361 aagagatgtt gttgatgtca tagcttcaaa cagcaatctt ttccgtatga tgtttgatgc
      421 taattaccag aacggatttc ggccgcaata tgcaaaaagg atagattctg aaactggaga
      481 aatgattgaa ggcgtgaata aacacgtcga ataaatatcg aaaacttccg ttaactgctt
      541 ggaatgaaaa aatagaatat tgggaaataa aaaacagata ggaataactc ctatctgttt
      601 tttatcgttt tagaaattta gttgggtcga aattgctaga agtgactgtt ttgtttgttc
      661 ctttttgatg attctttggt tgatttcgat ttgatggatt tagtttagct tcgatttttt
      721 ctttcacttc ttgatatact ttagaatttg tgccgataag aacgcctgta tcttttagat
      781 ataaatcagc gaattctgcg atgatttcag tcatgatgga aatttctttt cccatagcat
      841 ttagttttgc ctgattgctg gtagtggtcg tgttggtatc gggttcataa ctcatacata
      901 aaaagcgaat ggcttccgag tagttattga tatctggatt tttttcgatg atggaatcta
      961 aaacgtcaac atgactaggg tacaaagtaa catgtttttg gacagaagtt tttctcatgg
     1021 ttatatcatt cctccgataa gcatttcaat gatttgatta tttgtgtaga catactcgct
     1081 taatatttga gtattttctg tttggatagc aataaattta tcgagcaata aatcgaattg
     1141 gtcttttttg taagtcgtaa gttgacggtc aaaatcactt tcgattaaaa cttttagata
     1201 tttatttcga ctccattttt ctcctgtttt attcgtgagt tcttggcaac gtttgtctat
     1261 tgttgcaaca aactttggat caatattccg aactaaaatg tccataaaaa atctcctttt
     1321 gcaattatgt ataatttatg tataccatac aaatactata tttgtatagc tgttttttgc
     1381 caaaatacta tacataagct atccacgatg gcgaaaaagt gatatcaaat ttttttcaaa
     1441 gaaacgtgat aggcacagcc tatctagcac ctttcgtaag aaagtgatac cacgtttcct
     1501 tatgctcttt aaaattcggg gggagtgctg cacgtgtgat cgataaccaa atttaaaaat
     1561 gcacgatcaa aaatttattt agtttttcaa acgacatttt ttttgagagt atagacggca
     1621 aagaagtgat tttatttcta gaaaaattgt gagactggag aaagaaaaaa tggaaaaatt
     1681 tccttacgaa tattttcaaa acttagaaaa attagagcaa gcaattcaag caatcaagtt
     1741 cgatttaaca gacgaaaaat tgttgaaata taaaaatatg acgcccgaaa gaaaacgatc
     1801 gttggaaaaa ttaatgcaac tagtgaacga agacggcgga atttcagacg cagcttttga
     1861 cgcatggagc gaatttcatg gagaatataa ttaagtgggt ttaaatggct ctaaaatcgc
     1921 ttctaagagt tttttagctt ggaccaatga aatacaccaa aaactaaata aaacgtctta
     1981 aatcgaataa taatagcttt taaggaggcg atgggggatg gaaaaagata tttataaaca
     2041 attgacccga caaattcaaa aggaaaaaga aaaaggctta ctagatcatt tagatgaatt
     2101 agaactaaaa gctgatgaaa tcaataaaaa cagaatgtca gcaaaagaaa aaggtataaa
     2161 acttaaagat tcgtcgttgc aaagcgataa aagctagata acgtgcggtc aacggtaaat
     2221 cgaattagag acgcttagag aggctaataa gccctcttag cgtctctttg aggctttaag
     2281 gagttgacgg ctataaacaa aacccaaaag ctggaaacac aagaaggtga ccatagggaa
     2341 cgttcttgac gccttaacgg cgagtggaaa gtttttgggg tgcctttttt caaaaaggta
     2401 cggtcttgat ttgccgagcg atagcgctgg aaaatttttg gaaaaaattt ggaatttgga
     2461 aaatctgggg ggggctacta cgaccccccc catagtgtgc cgagtgccaa tttttgtgcc
     2521 aaaaagtgct ttatccttac tgtaccaatg gatagagcct ttttttttcg accgccattt
     2581 gaccgccgtt ttgaccgccg ttttgaaaaa tagaaaaaaa tattgacaat acagttgtca
     2641 aaagtttacc atgatattaa caattaacta aaggggctga aaggcatggc ggaatcagaa
     2701 agactggtag taattttacc gagtgaaatt aagcgaaaac ttgaggaaaa agcaaaagac
     2761 aaaagtttga gtatgtcgac atatgttaaa ttgctagtaa cagaagactt gaaaaaatca
     2821 gagaaaggaa attgagcgca aaaaaagagc agaaacatat gtgttggcgc accgtttccg
     2881 cttaactgat agtgttcaat tgaggtaagt atatcatgaa taagaatata acgaaagcta
     2941 gacattttgg ttttttacta tatcccgaat ctattccaga gaattggaga gaacagcttg
     3001 agagcttagg agtatcaatg gcggttagtc cgttacacga tatggacgaa aaaacaaata
     3061 aaaaggattg gactagtagt gatgttatca gaaacggtaa gcattatgta aagccacatt
     3121 atca
//
